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Mitochondrial health is critical for skeletal muscle function and is improved by exercise training through both mitochondrial biogenesis and removal of damaged/dysfunctional mitochondria via mitophagy. The mechanisms underlying exercise-induced mitophagy have not been fully elucidated. Here, we show that acute treadmill running in mice causes mitochondrial oxidative stress at 3–12h and mitophagy at 6h post-exercise in skeletal muscle. These changes were monitored using a novel fluorescent reporter gene, pMitoTimer, that allows assessment of mitochondrial oxidative stress and mitophagy in vivo, and were preceded by increased phosphorylation of AMP activated protein kinase (Ampk) at tyrosine 172 and of unc-51 like autophagy activating kinase 1 (Ulk1) at serine 555. Using mice expressing dominant negative and constitutively active Ampk in skeletal muscle, we demonstrate that Ulk1 activation is dependent on Ampk. Furthermore, exercise-induced metabolic adaptation requires Ulk1. These findings provide direct evidence of exercise-induced mitophagy and demonstrate the importance of Ampk-Ulk1 signaling in skeletal muscle.
It has been known since antiquity that exercise training improves physical performance and health. It was not until 50 years ago that the scientific community started to understand the importance of skeletal muscle biochemical and cellular adaptations in response to exercise1, 2. Skeletal muscle protein turnover (i.e., the culmination of protein synthesis and breakdown processes), particularly of mitochondrial proteins, plays a central role in mediating the health benefits of exercise training3–6 (Drake et al., 2015). The role of exercise training and the mechanisms controlling the synthesis of new mitochondria (i.e., mitochondrial biogenesis) have been extensively studied3, 7, 8, however, our understanding of how exercise promotes the removal of damaged and dysfunctional mitochondria is still in its infancy9.
Autophagy is a catabolic process that involves the sequestration of long-lived or damaged proteins and cytoplasmic organelles by a double-membrane structure called autophagosome10. During autophagy, an autophagosome then fuses with a lysosome to execute the degradation of the engulfed contents11. A low degree, basal level of autophagy appears to be responsible for maintaining cellular homeostasis and normal cell function12. Autophagy can, however, be upregulated under conditions of stress, such as in response to starvation and exercise13, 14. While it is generally believed that autophagy plays an important role in remodeling of skeletal muscle in response to exercise7, 14–16, the underlying molecular and signaling mechanisms remain obscure.
Four previous studies in mouse skeletal muscle showed that a single bout of exercise increased levels of the microtubule associated protein, light chain 3-II (Lc3-II; the phosphatidylethanolamine conjugated form of Lc3-I), a marker for autophagosome assembly. Altogether with decreased p62 content, a cargo receptor for ubiquitinated substrates that is also degraded during autophagy14, 17–19, it is suggestive of increased autophagy flux20 following exercise. Recently, we16 and others21 demonstrated that the autophagy machinery and markers for autophagy flux are also increased following endurance exercise training, suggesting increased basal autophagy flux and autophagy capacity. Of particular interest, we observed enhanced Bcl-2/adenovirus E1B 19KD interacting protein 3 (Bnip3) expression with exercise training16, which localizes to the outer mitochondrial membrane and presumably interacts with Lc3 to initiate mitochondria-specific autophagy (mitophagy)22. Finally, Vainshtein and colleagues demonstrated increased Lc3-II association with mitochondria and increased autophagy flux following exhaustive treadmill running in mice19.
Using a novel mitochondrial reporter gene, called pMitoTimer, we have shown that long-term high-fat diet induces mitochondrial oxidative stress and accumulation of damaged mitochondria in mouse skeletal muscle23, which might be due to a combination of increased lipid overload in mitochondria and stalled mitophagy. These findings illustrate the utility of MitoTimer in monitoring mitochondrial health in vivo. Additionally, the formation of pure red MitoTimer puncta co-localize with the mitochondrial protein Cox4 and the lysosomal protein Lamp1, indicative of mitochondria being selectively degraded through mitophagy23. Given that there is currently no direct evidence that exercise activates mitophagy or what the initial signals are that trigger mitophagy’s induction in response to exercise, we reasoned that pMitoTimer would allow for novel in vivo dissection of these outstanding questions.
The 5′-AMP-activated protein kinase (Ampk) has been extensively studied and highly implicated in skeletal muscle adaptation in response to exercise training24, 25. The link of Ampk to autophagy/mitophagy in skeletal muscle comes from data on muscle-specific Ampk knockout mice that have impaired induction of autophagy/mitophagy-related signaling in response to fasting13 and develop more pronounced mitochondrial abnormalities with aging13, 26. In fact, Ampk and a downstream regulator of autophagy/mitophagy, unc-51 like autophagy activating kinase 1 (Ulk1), have been shown to play critical roles in mitophagy in primary hepatocytes and erythrocytes27, 28. Ampk activates Ulk1 through direct physical interaction29 and phosphorylation at multiple sites30–32, including serine 555, which is important for activation, protein–protein interactions, and translocation of Ulk1 to the mitochondria28, 31, 33, 34. Furthermore, studies in mice and humans have shown that acute exercise stimulates both AMPK and Ulk1 phosphorylation18, 31, 35, 36. Altogether, these findings support an association between Ampk and Ulk1 in skeletal muscle, however, there has been no direct evidence, or mechanistic dissection of the signaling mechanism for Ampk and Ulk1 in control of mitophagy in skeletal muscle in response to exercise. Elucidation of Ampk-Ulk1 signaling in control of mitophagy could unveil one of the most important steps in exercise-mediated improvement in skeletal muscle mitochondrial health: removal of damaged/dysfunctional mitochondria.
We took advantage of a whole mount muscle imaging system using pMitoTimer in combination with the lysosomal marker gene, pLamp1-YFP (Lysosome Associated Membrane Protein 1)23, to obtain direct evidence of mitophagy in skeletal muscle in response to exercise. Furthermore, using mice with genetic alteration of the Ampk-Ulk1 signaling axis, including models with muscle-specific over-expression of either dominant-negative (α2 isoform) or constitutively-active (γ1 isoform) Ampk and muscle-specific knockout of Ulk1, we rigorously investigated the role of Ampk in activation of Ulk1 in response to exercise, as well as their individual functional roles in exercise-induced mitophagy. We found that Ampk is required for exercise-induced, site-specific phosphorylation of Ulk1 and this is required for the resolution of exercise-induced mitochondrial damage through mitophagy during recovery. Importantly, Ulk1 is required for metabolic adaptation to chronic exercise training. The findings have important implications for the roles of mitophagy and skeletal muscle mitochondrial quality control in the health and metabolic benefits of exercise training.
We assessed mitochondrial oxidative status and mitophagy in pMitoTimer-transfected adult mouse flexor digitorum brevis (FDB) skeletal muscle in a 24-h time course following a single bout (90min) of treadmill running. MitoTimer is an oxidation sensitive fluorescent protein, called Timer37, that targets to the mitochondria23, hence the name MitoTimer. MitoTimer fluoresces green when it is newly synthesized and shifts fluorescence to the red spectrum upon oxidation23, 37. Simultaneously imaging the green (488/518nm) and red (543/572nm) channels via confocal microscopy allows for assessment of overall oxidative status of the mitochondrial reticulum, reported as a red:green ratio23. Furthermore, using MitoTimer, the number of pure red mitochondrial puncta can be determined as an indication of degenerated mitochondria undergoing mitophagy, since we previously demonstrated that pure red puncta co-localize with both Cox4 immunostaining and Lamp1-YFP23. Here, we found that the red:green ratio of the mitochondrial reticulum was significantly shifted towards red fluorescence at 3, 6, and 12h following acute exercise (Fig. 1a, b, Supplementary Fig. 1A). This time-dependent shift toward red fluorescence, indicative of oxidative stress, is further supported by an increase in the lipid peroxidation marker 4HNE at 3 and 6h following acute exercise in mitochondria-enriched fractions from gastrocnemius muscle (Supplementary Fig. 2). In addition, the number of pure red puncta per fiber area was significantly increased only at 6h following acute exercise (Fig. 1c). We co-transfected WT mouse FDB skeletal muscle with pMitoTimer and the lysosome marker pLamp1-YFP, subjected them to treadmill running, and harvested FDB muscles 6h post-exercise, corresponding with the peak of mitochondrial oxidative stress and mitophagy (Fig. 1a–c, Supplementary Fig. 1). Importantly, we observed co-localization of pure red MitoTimer puncta with Lamp1-YFP 6h post-exercise (Fig. 1d). This is considered evidence of autophagosome-engulfed mitochondria that have fused with the lysosome, strongly suggesting that the increase in red puncta during recovery from acute exercise is due to selective degradation of mitochondria through mitophagy. Altogether, these findings provide the first direct evidence of exercise-induced mitophagy associated with increased mitochondrial oxidative stress.
To determine if these findings were specific to the mitochondrial reticulum, we transfected the contralateral FDB muscle with ER-Timer, an endoplasmic reticulum (ER) reporter analogous to pMitoTimer, but instead containing an ER targeting sequence fused to the Timer reporter gene. We found no change in red:green ratio or number of pure red puncta in the contralateral FDB muscle (Fig. 1e–g, Supplementary Fig. 1B). Furthermore, we found no change in protein levels of the ER-content marker Sec61a (Supplementary Fig. 3A, E). Altogether, these data support that the exercise-induced changes of pMitoTimer are organelle-specific.
Both the red:green ratio and number of pure red puncta (Fig. 1a–c, Supplementary Fig. 1A) in pMitoTimer transfected FDB muscles as well as levels of 4HNE in isolated mitochondria (Supplementary Fig. 2) returned to control levels by 24h post-exercise. These findings suggest acute exercise induces a transient mitochondrial oxidative stress along with a transient induction of mitophagy. It is reasonable to believe that activated mitophagy in response to exercise functions to remove certain parts of the mitochondrial reticulum that are severely stressed/damaged, aiding in the restoration of a healthy mitochondrial population within 24h of the exercise bout.
In order to further characterize mitochondrial degradation following acute exercise, we performed western blot analysis for mitochondria-specific proteins; the mitochondrial quality control protein, PTEN-induced putative kinase 1 (Pink1) (Supplementary Fig. 3A, D), as well as voltage dependent anion channel (Vdac) and translocase of outer membrane 20 (Tom20) (Supplementary Fig. 3A–C). We did not see changes in abundance of the above proteins in whole muscle lysates (Supplementary Fig. 3). We also quantified mitochondrial DNA (mtDNA) relative to nuclear DNA to obtain a measure of mitochondrial content. We did not find significant differences in mtDNA levels between sedentary and 6h post exercise (Supplementary Fig. 3F, G). No change in mitochondrial content is likely due to the relative small number of degenerated mitochondria undergoing mitophagy (as assessed by MitoTimer) in relation to the entire reticulum, and thus the above assays are not sensitive enough to detect such small changes.
Finally, in mitochondria-enriched fractions from gastrocnemius muscle, we observed increased Lc3-II 3h following treadmill running (Fig. 1h, i) but no change in Lc3-I at any time point, which is in line with previous reports19. The timing of Lc3-II recruitment is important since Lc3 should be degraded by lysosomal hydrolases following fusion of autophagosome and lysosome. Therefore, recruitment of Lc3 to mitochondria prior to MitoTimer co-localization with Lamp1-YFP supports the chronological order of mitophagy mechanisms and definitively shows exercise-induced mitophagy in skeletal muscle. This finding further highlights the utility of MitoTimer as a sensitive tool to detect individual organelle dynamics.
It is well known that Ampk can be activated by an acute bout of exercise38, 39. We found that treadmill running increased phosphorylation of Ampk at tyrosine (T) 172 and its downstream substrate Acetyl-CoA Carboxylase (Acc) at serine (S) 79 in gastrocnemius muscle immediately following the exercise bout (Fig. 2a–c). This effect was transient and not observed at later time points during the recovery period. Concurrently, acute exercise increased phosphorylation of Ulk1 only at the activating S555 site28 (Fig. 2a, d). The inhibitory phosphorylation sites of S467 or S75728 were unaltered (Fig. 2a, e, f). These findings suggest that exercise leads to a transient phosphorylation of Ampk and Ulk1 on activating sites, prior to mitophagic degradation of targeted mitochondria during recovery from exercise (Fig. 1a–c, Supplementary Fig. 1A).
Mitochondrial fission is essential to separate damaged/dysfunctional areas of mitochondria from the mitochondrial reticulum and thus facilitates mitophagy. The dynamin-related protein 1 (Drp1) is an important GTPase that promotes mitochondrial fission when phosphorylated at S61640. We found that phosphorylation of Drp1 at this site (S616) was transiently increased immediately following treadmill running (Fig. 2a, g). We also found that phosphorylation at site S637 was elevated following acute exercise, reaching significance 3 and 6 hours after the cessation of exercise (Fig. 2a, h). S637 phosphorylation has been shown to increase fission in response to Ca2+ signaling41, yet can also inhibit Drp1 activity under conditions of protein kinase A (Pka)-dependent phosphorylation42–44, and has been suggested as an Ampk target45, 46. This discrepancy in the literature highlights the importance of context specificity of molecular signaling. Finally, we found no alterations in the mitochondrial fusion protein Mfn2 across the 24h time course following acute exercise (Fig. 2a, i).
To determine whether Ampk is required for exercise-induced activation of Ulk1, we subjected muscle-specific transgenic mice of a dominant-negative form of the catalytic α2 subunit of Ampk (dnTG)47 to acute exercise. These mice were able to complete the exercise bout to the same capacity as the wild type littermates. Ampk phosphorylation at T172 was blunted in dnTG mice in response to exercise (two-way analysis of variance (ANOVA) interaction effect p=0.044, F=4.45, DF=1) (Fig. 3a, b); however, exercise-induced phosphorylation of Acc was not disturbed (Fig. 3a, c), as has previously been reported for this Ampkα mouse model and others48–50. Importantly, Ulk1 S555 phosphorylation was completely ablated in dnTG skeletal muscle following exercise (two-way ANOVA interaction effect p=0.015, F=6.71, DF=1) (Fig. 3a, d). Phosphorylation of Ulk1 at the inhibiting site S757 was unchanged by exercise in both WT and dnTG mice, however, there was a genotype effect of lower phosphorylation in the dnTG (Fig. 3a, e). Consistently, muscle-specific transgenic mice expressing a constitutively active form of the regulatory γ1 subunit of Ampk (caTG) showed an increased level of Ampk T172 phosphorylation (Fig. 3f, g)51 as well as a significant increase in Ulk1 phosphorylation at S555, but not S757, under sedentary conditions (Fig. 3f, i, j). These mice also displayed elevated Acc phosphorylation (Fig. 3f, h), providing additional evidence of increased Ampk activity. Altogether, these data demonstrate that Ampk activation is sufficient and necessary for exercise-induced Ulk1 S555 phosphorylation.
To investigate whether mitochondrial dynamics were regulated through Ampk, we also measured Drp1 phosphorylation and Mfn2 protein in dnTG and caTG Ampk mice. As observed in WT exercised mice, phosphorylation of Drp1 was significantly enhanced following acute exercise in Ampk dnTG mice at both S616 and S637 (Fig. 4a–c). In fact, phosphorylation of S637 was induced to a level almost twofold higher than that of WT mice by acute exercise (two-way ANOVA interaction effect p=0.007, F=8.69, DF=1) (Fig. 4c). Furthermore, under sedentary conditions, caTG mice displayed similar levels of Drp1 phosphorylation to their WT littermates (Fig. 4e–g) and there were no changes in Mfn2 protein, regardless of genotype or exercise (Fig. 4a, d, e, h). These findings suggest that exercise-induced phosphorylation of Drp1 occurs independently of Ampk in skeletal muscle.
Since Ampk is required for activation of Ulk1 by exercise (Fig. 4), we investigated whether exercise-induced mitophagy is dependent on Ampk. We performed co-transfection in FDB muscles with pMitoTimer and pLamp1-YFP in dnTG mice. As already described, dnTG mice were subjected to treadmill running and the FDB was harvested 6h post-exercise, corresponding with the peak of mitochondrial oxidative stress and mitophagy (Fig. 1, Supplementary Fig. 1). Consistent with our previous experiment, we observed an overall increase of red:green ratio 6h following exercise in WT mice (Fig. 5a, b). In contrast, dnTG mice showed moderately elevated oxidative stress, but there was no further increase following exercise (two-way ANOVA interaction effect p=0.037, F=4.92, DF=1) (Fig. 5a, b). The increase in MitoTimer pure red puncta 6h post-exercise observed in WT mice was completely abolished in dnTG mice (two-way ANOVA interaction effect p<0.0001, F=36.26, DF=1) (Fig. 5a, c), demonstrating that exercise-induced mitophagy is blocked in dnTG mice. Importantly, acute exercise caused an increase in Lamp1 puncta in WT mice, indicative of increased lysosomal biogenesis (Fig. 5a, d). This effect of exercise on lysosomal biogenesis was absent in dnTG mice (two-way ANOVA interaction effect p=0.019, F=6.35, DF=1) (Fig. 5a, d), which is in agreement with a recent report that Ampk deficiency dramatically reduces expression of the master gene of lysosomal biogenesis, Tfeb52. Consistent with the notion that Ampk is required for exercise-induced mitophagy, co-localization of Lamp1 puncta with pure red mitochondrial puncta was abolished in dnTG mice (two-way ANOVA interaction effect p=0.0002, F=20.08, DF=1) (Fig. 5e). This effect was also severely blunted when co-localized Lamp1 puncta were assessed as a percentage of total Lamp1 puncta (Fig. 5f). Altogether, these findings provide the first direct evidence that Ampk is required for exercise-induced mitophagy in skeletal muscle.
Since Ulk1 is a critical regulator of autophagy and is activated by acute exercise (Fig. 2), Ulk1 may be important for mitophagy to remove damaged/dysfunctional mitochondria during recovery in response to acute exercise (Fig. 1, Supplementary Fig. 1). To address this question, we again co-transfected FDB muscles with pMitoTimer and pLamp1-YFP in muscle-specific Ulk1 knockout (MKO) mice. As described above, mice were then subjected to acute exercise, and FDB muscles were harvested 6h later (Fig. 1, Fig. 6, and Supplementary Fig. 1). There was no difference in basal red:green ratio of MitoTimer between the WT and MKO mice (Fig. 6a, b). Furthermore, the overall increase of the red:green ratio 6h after exercise was similar between genotypes (Fig. 6a, b), indicating that deletion of Ulk1 did not alter the responsiveness of mitochondria to exercise with regard to oxidative stress. In WT mice, there was an increase in MitoTimer pure red puncta after exercise (Fig. 6a, c), which was concurrent with both increases in Lamp1 puncta (Fig. 6d) and co-localization between the two signals (Fig. 6e, f). These findings consolidate those from our previous experiments (Fig. 1 and Fig. 5), therefore, we are confident that mitophagy is increased during recovery in response to exercise. Furthermore, there was no increase in pure red puncta in skeletal muscle in MKO mice (two-way ANOVA interaction effect p=0.039, F=4.62, DF=1) (Fig. 6a, c) even though there was a significant increase of Lamp1 puncta above WT levels with exercise (Fig. 6d). These findings indicate that Ulk1 is not required for lysosomal biogenesis. Importantly, the co-localization of Lamp1 puncta with MitoTimer pure red puncta was dramatically impaired in MKO mice compared with WT mice (Fig. 6e, f). This was evident in both the co-localization of Lamp1 puncta per fiber area (two-way ANOVA interaction effect p=0.059, F=4.01, DF=1) (Fig. 6e) and percentage of co-localization to total Lamp1 puncta (two-way ANOVA interaction effect p=0.10, F=2.81, DF=1) (Fig. 7f). Altogether, these findings strongly demonstrate that Ulk1 is required for exercise-induced mitophagy and has an important role in targeting the lysosome to mitochondria selected for degradation.
Since we reported that Ulk1 is critical for acute exercise-induced mitophagy, we reasoned that it would also play a critical role in exercise training adaptation and endurance capacity. Therefore, we subjected MKO and WT mice to 6 weeks of voluntary wheel running and performed an exhaustive exercise test at the cessation of exercise training. Unexpectedly, daily voluntary running distance was similar between genotypes, and both genotypes improved their endurance running capacity after 6 weeks of voluntary running compared to sedentary controls (Fig. 7a, b), suggesting that Ulk1 is not required for enhanced physical performance in response to exercise training. In order to further validate these findings and rule out developmental compensation to loss of Ulk1 from early life, we generated tamoxifen-inducible, skeletal muscle-specific Ulk1 knockout (iMKO) mice and subjected them to the same exercise training protocol after 5 days of tamoxifen injection (to delete the Ulk1 gene in skeletal muscle). Consistently, iMKO mice demonstrated similar daily voluntary running distance compared with WT mice, as well as similar improvement in endurance capacity following 6 weeks of voluntary exercise training (Fig. 7d, e). Blood lactate concentration changes with exercise were consistent with exhaustion and improved metabolic adaption with exercise in both genetic mouse models (Fig. 7c, f); however, iMKO mice had significantly higher blood lactate than wild type upon exhaustion (Fig. 7f, Supplementary Fig. 5).
To further investigate the importance of skeletal muscle Ulk1 in exercise training-induced adaptation, we performed glucose tolerance tests (GTTs) in WT and iMKO mice following 4 weeks of voluntary wheel running. WT mice that performed exercise training displayed significantly better glucose tolerance compared to their sedentary counterparts as well as exercise trained iMKO mice, as indicated by improved blood glucose clearance during GTT and area under the glucose curve (two-way ANOVA interaction effect p=0.001, F=19.52, DF=1) (Fig. 7g–i). In contrast, iMKO mice showed no improvement in glucose tolerance following 4 weeks of exercise training (Fig. 7h, i). Altogether, these data suggest that while skeletal muscle Ulk1 may not contribute to improvements in endurance capacity, Ulk1 in skeletal muscle is important to confer the metabolic benefits of exercise training.
Our data provides the first direct evidence that a single bout of exercise induces mitophagy in skeletal muscle during recovery. We observed increased mitochondrial oxidative stress between 3–12h with increased MitoTimer pure red puncta 6h following acute exercise, suggesting that upstream signaling mechanisms were initiated prior to the mitophagy events. Consistent with this notion, we observed temporal activation of Ampk and Ulk1 immediately after acute exercise and demonstrated that Ampk was both necessary and sufficient for phosphorylation of Ulk1 at S555 in skeletal muscle. Importantly, we have obtained comprehensive evidence that both Ampk and Ulk1 are required for exercise-induced mitophagy during recovery. While Ampk is involved in Ulk1 activation and lysosomal biogenesis, our data does not support the notion that Ampk promotes mitochondrial fission through Drp1. Finally, we demonstrate the novel finding that Ulk1 is required for mitophagy at the step of lysosomal targeting of damaged/dysfunctional mitochondria (Fig. 8). Hence, we show for the first time that mitophagy during recovery from exercise in skeletal muscle is dependent upon the transient activation of Ulk1 through Ampk.
Mitochondria are the main source of reactive oxygen species (ROS), primarily through leak of single electrons from the electron transport chain during oxidative phosphorylation53, 54. During exhaustive exercise, free radical (e.g., reactive oxygen species, or ROS) concentrations within the mitochondria increase55, disrupting redox homeostasis and causing oxidative stress56. Oxidation of mtDNA, respiratory chain complexes, and/or lipids are thought to be the primary targets of free radicals, resulting in mitochondrial dysfunction and initiating mitochondrial maintenance adaptation55, 57, such as through their removal and degradation7. In the current study, the overall shift in MitoTimer red:green ratio is indicative of an increase in mitochondrial oxidative stress 6h post exercise. In line with this observation, 4HNE, a common marker of oxidative stress, was also increased in mitochondria-enriched fractions at 3 and 6h post exercise. These data are consistent with the notion that during recovery from exercise, continued oxidative phosphorylation and dramatic reduction of ATP consumption dominate the excessive production of mitochondrial ROS. These observations are important for development of future interventions to improve muscle recovery from an acute exercise bout.
It is worth mentioning that acute exercise did not lead to oxidation of the Timer protein when it was targeted to the endoplasmic reticulum (ER-Timer). Although others have shown that acute endurance exercise activates ER-stress in skeletal muscle58, 59, we must highlight that ER-Timer detects only oxidation of the Timer protein and not specific activation of the unfolded protein response (UPR). Although changes in cellular redox can indeed cause ER-stress, aberrant Ca2+ regulation and glucose deprivation have also been implicated in initiating the UPR60. Since ER oxidative status was not measured in studies reporting exercise-induced ER-stress58, 59, we caution against the over interpretation of our findings with ER-timer as counter to the current literature.
While we have demonstrated here that Ampk and Ulk1 are indeed required for mitophagy in skeletal muscle within the context of exercise, the mechanism of how the mitochondrial damage signal and activation of mitophagic machinery is coordinated has yet to be fully elucidated. Mitochondrial damage causes the accumulation of Pink1 on the mitochondrial outer membrane, recruiting Parkin. Parkin subsequently ubiquitinates mitochondrial proteins61, 62, initiating the separation of that region of the reticulum through fission upon accumulation, then leading to degradation through mitophagy62, 63. While exercise training increases Pink1 mRNA in human skeletal muscle64 and Parkin protein content deceases following exercise in mice65, these findings are far from conclusive. We did not find significant changes in Pink1 protein in skeletal muscle following acute exercise. We cannot rule out a role for Pink1 in exercise-induced mitophagy. In our hands, it is just as likely that we did not detect changes in Pink1 using western blotting because of the small fraction of “ear marked” red mitochondrial puncta. Whether Ampk/Ulk1 interact with Pink/Parkin to facilitate mitophagy in response to exercise or through another, unknown mechanism, to distinguish damaged mitochondria remains an outstanding question.
We noticed that the pure red puncta are always dissociated from the mitochondrial reticulum in skeletal muscle (Figs. 1, ,5,5, ,66 and Supplementary Fig. 1). This is consistent with the notion that selective mitophagy in regions of the mitochondria that are dysfunctional require mitochondrial fission. Interestingly, Ampk has also been shown to promote fragmentation of the mitochondrial network and directly phosphorylate the mitochondrial fission factor (Mff), which is a Drp1 receptor required to recruit Drp1 to the mitochondrial membrane66. In support of this concept, Vainshtein and colleagues reported increase mitochondrial associated Drp1 immediately following exhaustive exercise in mice19. We also found that phosphorylation of Drp1 was initiated immediately following acute exercise in the skeletal muscle, supporting the notion of targeted mitochondrial fission prior to mitophagic degradation. However, whether Drp1 was specifically associated with damaged or dysfunctional mitochondrial remains unknown. In contrast to some reports45, 46, in our hands Ampk does not appear to be responsible for phosphorylation of Drp1 at either S616 or S637 since we observed significant increases in phosphorylation levels after acute exercise in dnTG mice. However, one could speculate that Ampk plays a role in fine-tuning of Drp1 and mitochondrial fission after exercise since phosphorylation of S637 in dnTG mice was further enhanced above WT levels after exercise.
Importantly, in mice lacking functional Ampk (dnTG) in skeletal muscle we observed a tendency for elevated MitoTimer red:green ratio, even at the basal level. These observations may be reflective of Ampk’s role in regulation of antioxidant defense67, 68, and suggests a basal elevation of mitochondrial oxidative stress. However, there was no further increase in the red:green ratio following acute exercise suggesting that mitochondrial ROS production plateaued at the basal level in dnTG skeletal muscle and the acute exercise caused no further elevation. In the MKO mice, however, there was no elevation of MitoTimer red:green ratio, at the basal level, suggesting that deletion of the Ulk1 gene, unlike inhibition of Ampk, does not cause mitochondrial oxidative stress.
On the other hand, MKO mice displayed a similar phenotype of lacking increased pure red puncta in response to exercise. This impaired mitophagy does not necessarily indicate that there are no damaged mitochondria, particularly in light of elevated overall MitoTimer red:green ratio after exercise. Instead, these observations are indicative of defective targeting of the autophagic machinery to the specific damaged mitochondria. This notion is supported by studies that identified a critical interaction between Ulk1 and the scaffold protein Htt, which is required for selective autophagy (including mitophagy), but not non-selective autophagy69. Rui and colleagues show that the physical interaction of Htt with Ulk1 is required to guide Ulk1 to the site of autophagosome formation, and the loss of Htt causes impaired autophagosome sequestration of selective cargo69. These observations are consistent with our current findings when Ulk1 was specifically depleted in skeletal muscle. Therefore, in the absence of Ulk1, the selective targeting of Htt to the site of autophagosome formation may either be impaired or ineffective since the role of Ulk1 in regulating autophagosome formation may not be realized. The acute consequence of which is the defective sequestration of selective autophagic/mitophagic targets and potential accumulation of damaged organelles in the cell. Indeed, it appears that Ulk1 may play an important role in exercise training-induced metabolic adaptation since iMKO mice showed no improvement in glucose tolerance following 4 weeks of voluntary wheel running and limited adaptation in lactate metabolism during an exhaustive running test. Therefore, Ulk1 could be a critical regulator for proper metabolic regulation during other metabolic challenges (e.g., resistance exercise, high-fat diet, and aging).
In summary, we demonstrate for the first time that acute exercise induces mitophagy through Ampk-dependent activation of Ulk1 in skeletal muscle. Furthermore, deletion of the Ulk1 gene in skeletal muscle inhibits mitophagy without affecting lysosome formation in response to exercise, which suggests to us a novel role for Ulk1 in targeting of lysosomes to damaged/dysfunctional mitochondria that has implications for metabolic adaptations to exercise training.
All experimental procedures were approved by the University of Virginia, Institutional Animal Care and Use Committee. For post-exercise time course experiments, male C57BL/6J mice (10–12 weeks old) were obtained commercially (Jackson Laboratories). For assessing mitophagy-related signaling basally, immediately post-exercise, and 6h post exercise, male muscle-specific, dominant-negative (dnTG) Ampk α2 (12–13 weeks old)47 and male constitutively-active (caTG) Ampk γ1 (13–15 weeks old)51 were bred and raised in house at the University of Virginia. MKO and iMKO mice were obtained by crossbreeding between Ulk1 f/f 27 and MCK-Cre (Jackson Laboratory) and between Ulk1 f/f and HAS-MerCreMer mice (generous gift of Dr Karyn Esser), respectively. For examining mitophagy 6h post exercise and following exercise training, MKO mice (12–13-weeks old) and iMKO mice (18–20-weeks old) were bred in house. Prior to use, iMKO were injected i.p. with tamoxifen (1mg/25mg body weight) once daily for 5 days to induce knock out of the Ulk1 gene. All mice were housed in temperature-controlled (21°C) quarters with 12:12-h light-dark cycle and ad libitum access to water and chow (Purina). We chose to study the FDB muscle located in the base of the hindlimb foot. This decision was primarily based on the practical advantages of the location and size of the muscle for ease of plasmid transfection and whole mount imaging. In addition, the FDB muscle is known to be recruited during exercise and adapts to exercise training23 and displays increased Ampk activity in response to ex vivo contraction70, 71. The FDB is composed of mostly type IIa fibers, which are fast, oxidative and contain an intermediate sized mitochondrial pool and autophagy protein abundance is also intermediate, between type I and IIb muscle types16. Therefore, it is an ideal muscle for the purposes of this study.
We designed and validated pMitoTimer as a useful tool for measuring mitochondrial health and damage as previously published23. pLamp1-YFP was purchased from Addgene (Plasmid #1816). Plasmid constructs were transfected into the FDB muscle by somatic gene transfer as previously published23, 72. Briefly, the mice were anesthetized (isofluorane) and the base of each hind limb foot, where the FDB is located, was injected with 10μl of hyaluronidase (0.36mg/ml; Sigma) subcutaneously. One hour later the mice were anesthetized a second time and the FDB injected with 20μg of plasmid DNA. In cases where pMitoTimer and pLamp1-YFP were co-transfected, they were mixed in a 1:1 ratio and 20μg of total plasmid DNA were injected. Ten minutes later a pair of gold-plated acupuncture needles were inserted under the skin at the heel and base of the toes parallel to each other and perpendicular to the long axis of the foot. Ten pulses with 20ms duration at 1hz and 75V/cm were applied to each foot. To allow for the mice to recover and plasmid expression to be stable we allowed 10 days before performing any experiments.
At the time of muscle harvest, the FDB was immediately fixed in 4% paraformaldehyde for 20min and whole mounted on gelatin coated slides with 50% glycerol in phosphate-buffered saline with a coverslip. Images were acquired at ×100 magnification under a confocal microscope (Olympus Fluoview FV1000) using the green (excitation/emission 488/518nm) and red (excitation/emission 543/572nm) channels with identical pre-determined acquisition parameters for all samples to ensure no saturation of the signals and similar intensity of the green and red channels in control samples. Lamp1-YFP images were acquired using the yellow channel (excitation/emission 515/527). MitoTimer signals were analyzed using our custom-designed Matlab-based algorithm as previously published23. Co-localization of pure red MitoTimer puncta and yellow Lamp1 puncta was quantified by an updated algorithm.
Mice were acclimatized to the treadmill for 3 consecutive days prior to the experimental day. Acclimatization consisted of 10min of treadmill running at 0% incline and speed of 13m/min each day. On the day of acute exercise, mice allocated to perform treadmill running were subject to 5% incline and 10min at 13m/min, 10min at 16m/min, 50min at 19m/min, and then 20min at 21m/min. Mice were sacrificed at the indicated time points following treadmill running.
Ulk1 MKO, iMKO and WT littermates were housed individually in cages with running wheels and sedentary mice we kept together in cages not equipped with running wheels. Exercising mice were allowed free access to the running wheel and all mice were provided food and water ad libitum. Daily running distance was recorded via computer monitoring. In iMKO mice, after 4 weeks of voluntary wheel running, running wheels were locked for 24h and mice were subjected to a GTT. Following a 6h fast, blood glucose from the tail vein was measured prior to and following i.p. injection of sterilized d-glucose (2mg/g) at 30, 60, and 120min. Running wheels were subsequently unlocked and one week later mice were subjected to an exhaustive exercise test. Mice were acclimatized to the treadmill as described above. On the day of testing the treadmill was set to 5% incline and run at 13m/min for 30min. Running speed was increased by 3m/min every 30min until perceived exhaustion. Blood lactate was measured prior to and at the time of exercise cessation.
Total protein or mitochondrial-enriched lysates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membrane. Mitochondrial-enriched lysates were isolated via differential centrifugation from frozen gastrocnemius muscle. Briefly, muscles were pulverized by mortar and pestle and placed in 1ml fractionation (FRAC) buffer [20mM HEPES, 250mM Sucrose, 0.1mM EDTA, plus protease (Roche Diagnostics) and phosphatase (Sigma) inhibitors] then sonicated. Whole-tissue lysates were then spun at 800×g for 10min at 4°C. Supernatants were then spun at 9000×g for 10min at 4°C. The resulting mitochondrial pellets were resuspended in FRAC buffer then spun at 11,000×g for 10min at 4°C. Resulting mitochondrial-enriched pellets were resuspended in 50μl of lamelli buffer, boiled for 5min at 97°C, then frozen at −80°C until further analysis.
Membranes were probed with the following primary antibodies at 1:1000 dilution, targeting Lc3A/B (CST #4108), T172 Ampk (CST #2535), Ampk α1/2 (CST #2532), Ampk γ1 (Abcam #32508), S79 Acc (CST #3661), Acc (CST #3662), S555 Ulk1 (CST #5869), S757 Ulk1 (CST #6888), S467 Ulk1 (CST #4634), Ulk1 (Sigma-Aldrich #A7481), S616 Drp1 (CST #3455), S637 Drp1 (CST #4867), Drp1 (CST #8570), Vdac (CST #4866), Tom20 (CST #42406), Mfn2 (CST #56889), Pink1 (Santa Cruz #33796), Sec61a (CST #14867), 4HNE (Abcam #48506), α-Tubulin (mouse, Abcam #11304), and Gapdh (CST #2118). All antibodies are sourced from rabbit unless otherwise noted above. All blots were cut at desired kDa ranges depending upon the predicted location for proteins of interest after Ponseau-S staining to maximize the utility of each blot by probing for multiple proteins, with the same source, at once. secondary antibodies were goat anti-mouse IR680 and goat anti-rabbit IR800 (LICOR). Membranes were scanned using the Odyssey infrared imaging system (LICOR). Proteins were analyzed in comparison to a common protein standard loaded on gel, prior calculating phospho:total ratio. All uncropped scans can be found in Supplementary Figs. 5–11.
DNA was isolated from gastrocnemius and plantaris muscles using standard DNA isolation procedures, and real-time qPCR was used to quantify mtDNA (Cytochrome B) and expressed relative to nDNA (Gapdh). Sequences of primers used are as follows; Cytochrome B: (f) 5′—ACG TCC TTC CAT GAG GAC AA—3′; (r) 5′—GGA GGT GAA CGA TTG CTA GG—3′ and Gapdh: (f) 5′—ACC CAG AAG ACT GTG GAT GG—3′; (r) 5′—CAC ATT GGG GGT AGG AAC AC—3′.
Data are presented as the mean±SEM. Time course experiments are analyzed via one-way ANOVA with Newman-Keuls post-hoc analysis. Where two variables are present, data was analyzed using two-way ANOVA with Tukey post-hoc analysis where appropriate. Where one variable is present, data was analyzed using Student’s t-test. Data with significantly unequal variance was transformed prior to statistical analysis. Statistical significance was established a priori as p<0.05.
The data generated in this study are available from the corresponding author upon reasonable request.
This work was supported by NIH (R01-AR050429) to Z.Y., AHA post-doctoral fellowship (14POST20450061) to R.C.L., NIH (T32 HL007284-38) and ADA post-doctoral fellowship (1-16-PDF-030) to J.C.D. NIH (T32 HL007284-37), AHA pre-doctoral fellowship (114PRE20380254) to R.J.W, NIH (R01-DK101043) to L.J.G., and NIH (5P30 DK36836) to Joslin Diabetes Center. We thank Dr Karyn Esser for the generous gift of HAS-MerCreMer mice. We also thank Dr David F. Kashatus, the members of the Yan and Hoehn labs for critical feedback and discussion.
R.C.L. designed the study, performed experiments, analyzed data, and wrote the manuscript, J.C.D. designed the study, performed experiments, analyzed data, and wrote the manuscript. R.J.W. performed experiments, provided technical assistance, and edited the manuscript, V.A.L. designed the study and analyzed data, K.A.R. and J.J.S. designed the MATLAB program and provided expert computational advice, M.Z. performed experiments and provided technical assistance, B.M.L. and C.C.F. provided technical assistance, L.J.G. and M.K. provided mouse models, expert advice, and critiqued the manuscript, and Z.Y. designed the study, analyzed data, and wrote the manuscript.
The authors declare no competing financial interests.
Rhianna C. Laker and Joshua C. Drake contributed equally to this work.
Electronic supplementary material
Supplementary Information accompanies this paper at doi:10.1038/s41467-017-00520-9.
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