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Patients receiving darunavir are advised to take it concomitantly with food. The objectives of the present cross‐sectional study were to evaluate the actual concomitant food intake of patients visiting an HIV outpatient clinic.
Sixty participants treated with darunavir/ritonavir once daily were subjected to a food recall questionnaire concerning their last concomitant food intake with darunavir. Darunavir trough concentrations were calculated.
The median food intake was 507 (0–2707) kcal; protein intake, 20 (0–221)g; carbohydrate intake, 62 (0–267)g; fat intake: 14 (0–143)g; and dietary fibre: 4 (0–30)g. Twenty‐five patients (42%) ingested their drug with between‐meal snacks. No relationship was found between food intake and trough concentrations.
Clear advice on the optimal caloric intake is needed, to avoid high caloric intake in patients who already have an increased risk of cardiovascular disease due to their HIV infection.
Darunavir (DRV) is a protease inhibitor (PI) that is administered with low‐dose ritonavir (RTV) to provide a pharmacokinetic boost by inhibiting drug metabolism, thereby enhancing plasma concentrations over time 1. Although DRV is considered to be a safe and efficacious drug, a considerable pharmacokinetic variability has been observed 2.
The observed variability may be partly explained by a food effect. In a prior study assessing the food effect on the bioavailability of DRV 400 mg (with RTV) in healthy volunteers, the bioavailability increased by 30% with food intake compared with the fasting state, and no significant differences were observed between the different diets tested 3.
Partly because of this food effect study, patients using DRV are advised to ingest their drug concomitantly with food. However, there is no clear‐cut advice on how much nutritional content (e.g. number of calories, and amount of fat, protein and carbohydrate) a meal should contain. The patient product brochures seem to focus more on caloric intake than on a healthy diet, as meals with a high caloric value are often recommended 4, 5, 6. As earlier studies were conducted in controlled settings, little attention was paid to the clinical implications in a real‐life outpatient setting. The primary objective of the present study was to determine how people living with HIV, using once‐daily DRV/RTV cope with the concomitant food intake advice given by their care givers and patient product brochures. Same‐day DRV trough concentrations (Ctrough) were measured and compared with cut‐off values used in clinical care.
In the HIV outpatient clinic of the University Medical Center Groningen (UMCG), patients with an appointment between 23 April 2014 and 28 July 2014 were asked to participate if they were using DRV/RTV 800/100 mg once daily. Consenting participants were subjected to a validated and structured food recall questionnaire, filled in by a trained researcher (A.D.) to record participants’ food intake (±30 min) with the last DRV administration before their appointment and the time of DRV ingestion. After performing diagnostic tests for standard care, residual blood was analysed to determine the DRV plasma concentration. The blood was drawn from the patient on the same day that the food intake questionnaire was obtained. Data concerning patient characteristics, blood chemistry (e.g. renal and hepatic function) and disease‐specific results (CD4+ cell count and viral load) were extracted from the medical records. We aimed to enrol 60 patients within the study period, as this sample size was estimated to provide a representative overview of the concomitant food intake. Written informed consent was obtained from all participants prior to any study procedure. The ethical review board of the UMCG reviewed the study and concluded that it was in accordance with Dutch law (METc 2014.115).
The validated food recall questionnaire consisted of the time of food intake, food preparation and an accurate description of the food and drink consumed. To assess the food quantity, the researcher used household measures and photographic tools, using the double‐check method on products such as milk, sugar and spices that are known to be under‐reported in such questionnaires. The Dutch National Food Consumption Survey 2012–2016 was used as a reference, in addition to the questionnaire used, to determine the type of meal (breakfast, lunch or dinner, or a between‐meal snack 7. The food recall questionnaire was analysed by D.D. using EvryDietist, 188.8.131.52 (Nevo 2011 data, Evry bv, Alphen aan den Rijn, Netherlands). The following nutritional values were calculated: energy (kcal), protein (g), carbohydrate (g), total fat (g) and dietary fibre (g). During the second half of the study, four questions were added to the food recall questionnaire in order to optimize the interpretation of the food intake. The first three additional questions asked were:
In the fourth question, we asked if patients changed their food pattern as a consequence of DRV and its concomitant food intake advice. We disregarded this question; interpretation of the answer was not possible without information on the antiretroviral therapy taken before the start of the DRV administration.
Participants were asked at which time point DRV was ingested. The time of blood sampling was recorded. The concentrations of DRV in human plasma were analysed using a validated liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. All analyses were performed on a Thermo Fisher Scientific Inc. (San Jose, CA, USA) triple quadrupole LC–MS/MS with a FinniganTM Surveyor® LC pump and a FinniganTM Surveyor® autosampler. The mobile phase consisted of an aqueous buffer (containing ammonium acetate 5 g l–1, acetic acid 35 ml l–1 and trifluoroacetic anhydride 2 ml l–1 water), water and acetonitrile and had a flow rate of 0.3 ml min–1. The calibration curves were linear within the concentration range 0.335–33.5 mg l–1 for DRV and had a correlation coefficient (R2) of 0.999. The lower limit of quantification (LLOQ) for DRV was 0.27 mg l–1. This method is precise and accurate: within‐day precision ranged between 2.2% and 3.2% for DRV, and between‐day precision from 3.0% to 5.2%. The calculated accuracy ranged from 0.0% to 11.8%.
The DRV Ctrough was defined as the plasma concentration at 24 h after intake of the dose. To estimate the Ctrough, we used a DRV iterative two‐stage Bayesian population pharmacokinetic model using the software package MWPharm Research version 3.82 (Mediware, Groningen, the Netherlands) 8. The model for DRV is a one‐compartment model with input and elimination from the central compartment. Parameters for this model are: a volume of distribution of the central compartment of 2 l kg–1 [standard deviation (SD) 0.5 l kg–1], total body clearance of 6.3 l h–1 1.85 m–2 (SD 1.57 l h–1 1.85 m–2), first‐order absorption constant of 1 h–1 (SD 0.25 h–1) and a bioavailability of 0.8 (in combination with RTV). This model was built in‐house and derived from data provided in the literature 9. A median population pharmacokinetic curve was used as a cut‐off value for follow‐up as in standard care 10, 11. A DRV Ctrough below 1.07 mg l–1 is an indication for follow‐up, in accordance with the treatment protocol. The median population pharmacokinetic curve is seen as a cut‐off value for the once‐daily dosage and not as the minimally effective concentration.
Further, the medical records of all participants were studied for medication potentially influencing the DRV concentrations.
Ctrough levels vs. the calculated kcal, carbohydrate, protein, total fat and dietary fibre values were presented in a scatter plot. Curve estimation tests were performed to find the best fit. All descriptive analyses were performed using SPSS for Windows, version 22.0 (IBM SPSS, Chicago, IL, USA).
Sixty patients were enrolled, of whom 50 were male. Participant demographic characteristics are presented in Table 1. Forty‐seven per cent of the participants were overweight [body mass index (BMI) ≥25 kg m–2], of whom 13% were classified as obese (BMI ≥30 kg m–2).
The medians of the calculated nutritional values for the meal concomitantly ingested with DRV are shown in Table 2. Eleven participants ingested DRV with breakfast, even with lunch, 14 with dinner and three without concomitant food, and 25 participants took their DRV with a between‐meal snack.
Twenty‐eight (85%) of the participants interviewed about the advice received at start of the treatment confirmed that the care provider advised them to eat concomitantly with the ingestion of DRV. Twenty‐four participants (73%) indicated that they did not know the amount of food intake recommended with DRV ingestion.
The median (interquartile range) DRV Ctrough for the 60 participants was 2.3 (1.51–3.67) mg l–1. Seven participants (12%) had a DRV Ctrough below the used cut‐off value of 1.07 mg l–1. No pattern could be detected in the DRV Ctrough and the caloric intake. A biologically expected S‐curve did not fit the data (P = 0.260), as presented in Figure 1. A linear model fitted slightly better compared with the other curve estimations but still showed no correlation (rho = −0.178, P = 0.173). Similar results were found for the other nutritional values (protein, carbohydrate, total fat and dietary fibre; not shown). The medical records of the participants did not show use of medication interacting with DRV/RTV.
To the best of our knowledge, this was the first study to evaluate the concomitant food intake in people using the once‐daily DRV dosage in a real‐life setting. Our findings showed that people using DRV often have a unnecessarily high caloric intake and that a large number of the patients take DRV with high caloric between‐meal snacks.
Patients using DRV are advised to ingest their drug concomitantly with food, although detailed advice on the type of food and number of calories is not given. This is reflected in the current study as the concomitant food intake among participants varied greatly and no relationship was found with DRV Ctrough. The high BMI (>25 kg m–2) of participants in the present study may partly be a consequence of the between‐meal snacks and subsequent high caloric intake. The use of antiretroviral therapy has been associated with a higher risk of cardiovascular and metabolic disorders, such as hyperlipidaemia, insulin resistance, metabolic syndrome and diabetes 13, 14, 15, 16. Therefore, it is important to ensure that the advice on concomitant food intake while using DRV does not lead to an unnecessarily higher caloric intake. Based on the findings by Sekar et al. 3 and our findings, we suggest that much of the food advice shown in the DRV patient brochures can be adapted to healthier dietary advice 4, 5.
Due to the observational nature of the study, it is possible that a recall bias on food intake was introduced, despite taking a careful history using a validated food recall questionnaire. Furthermore, the Ctrough was estimated using one blood sample, which could have given a distorted view. However, repeated blood samples would alter the actual (cross‐sectional) study design, and the use of Bayesian estimation in combination with patient characteristics, dosage and time of ingestion is a widely accepted method in daily practice to interpret drug level results 8, 17. Despite potential weaknesses, the results of the present study provide a good insight into the daily concomitant food intake in patients.
A controlled food effect study in patients is needed to optimize recommendations on the minimal amount of concomitant food intake to prevent unnecessary high‐caloric and high‐fat food intake in a patient group with already increased risks for cardiovascular and metabolic diseases.
There are no competing interests to declare.
A.D., W.F.W.B., J.G.W.K., J.W.C.A. and Y.S. were responsible for the concept and design of the study. The acquisition of laboratory and clinical data was performed by A.D., D.D., D.A.W. and T.S.W. The data was analysed by A.D. and Y.S. Both the drafting and the later critical revision of the article was conducted by A.D., T.S.W., J.W.C.A. and Y.S. The final approval of the manuscript was done by all authors.
Daskapan A., Dijkema D., de Weerd D. A., Bierman W. F. W., Kosterink J. G. W., van der Werf T. S., Alffenaar J.‐W. C., and Stienstra Y. (2017) Food intake and darunavir plasma concentrations in people living with HIV in an outpatient setting. Br J Clin Pharmacol, 83: 2325–2329. doi: 10.1111/bcp.13366.