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Commun Integr Biol. 2017; 10(4): e1338990.
Published online 2017 July 5. doi:  10.1080/19420889.2017.1338990
PMCID: PMC5595415

Keep it on the edge: The post-mitotic midbody as a polarity signal unit

ABSTRACT

The maintenance of the epithelial architecture during tissue proliferation is achieved by apical positioning of the midbody after cell division. Consequently, midbody mislocalization contributes to epithelial architecture disruption, a fundamental event during epithelial tumorigenesis. Studies in 3D polarized epithelial MDCK or Caco2 cell models, where midbody misplacement leads to multiple ectopic but fully polarized lumen-containing cysts, revealed that this phenotype can be caused by 2 different scenarios: the loss of mitotic spindle orientation or the loss of asymmetric abscission. In addition, we have recently proposed a third cellular mechanism where the midbody mislocalization is achieved through cytokinesis acceleration driven by the cancer-promoting phosphatase of regenerating liver (PRL)-3. Here we critically review these findings, and we furthermore present new data indicating that midbodies themselves might act as signal unit for polarization since they can infer apical characteristics to a basal membrane.

KEYWORDS: cancer, cell polarity, cytokinesis, epithelia, midbody, PRL-3, PTP4A3

The epithelium is a fundamental tissue in metazoans that acts as a selective permeable membrane for most of the animal organs. It consists of a layer of tightly attached polarized cells that line from the entire outside of the organism until internal hollow small lumens. Its characteristic architecture must be strictly regulated since defects on the specific architecture are the source of almost 90% of all human cancers.1-3 Thus, epithelial tissue polarization and organization have been extensively studied in 3-dimensional (3D) cellular models like single hollow lumen-containing 3D MDCK (Madin-Darby Canine Kidney) or Caco-2 (caucasian colon adenocarcinoma) cysts and in branched tubular MCF-7 (caucasian breast adenocarcinoma) structures.4-10

In MDCK cysts, a lumen is formed de novo by cord hollowing and starts during the first mitosis where vesicles containing apical markers move along the mitotic spindle microtubules to the midbody,4,11-13 a structure created within the cleavage furrow near the end of cytokinesis.14,15 Then, the Apical Membrane Initiation Site (AMIS) is formed, where the apical membrane will finally be positioned and the lumen will be opened.8,12,13 This process is independent of a preexisting apical-basal axis since the AMIS is formed around the midbody.8,12 Therefore, in subsequent cell divisions the midbody must be placed in the already specified apical membrane to ensure that a central single lumen is maintained.8 The apical positioning of the midbody is driven through a 2-step process. First, the mitotic spindle is oriented perpendicular to the apical-basal axis generating a radial cleavage furrow. Then, this furrow ingresses from the basal and the apical membrane asymmetrically toward the lumen to finally locate the midbody at the apical membrane at the end of the abscission (Fig. 1, pathway on the top).5,8,12,13,16 Disruption of any of these steps would result in mislocalized midbodies that would open ectopic lumens leading to the disturbance of the epithelial architecture (Fig. 1).8,16 Two mechanisms had been previously proposed in the literature: 1) the loss of the perpendicular spindle orientation, which leads to lateral instead of apico-basal furrow ingression (Fig 1, process labeled ‘1’); and 2) the loss of asymmetric abscission, where the midbody stays in the lateral membrane after cytokinesis (Fig. 1, process labeled ‘2’). The loss of spindle orientation has been extensively studied,8,13 but the symmetric furrow ingression remains as a theoretical scenario empirically suggested by forced recruitment of E-Cadherin to the basal membrane.8,13,17 Recently, we have suggested a third alternative cellular mechanism caused by the accelertion of the cytokinetis pace, leading to abscission taking place before the midbody reaches the apical membrane, and lateral retention of the midbody (Fig. 1, process labeled ‘3’).18 Here, we provide a critical review of that work and cast light on the relevance of the midbody in cell polarity by proposing it as a polarity signal unit.

Figure 1.
Mechanisms for ectopic lumen formation through post-mitotic midbody mispositioning. Pathway on the TOP: At early cystogenesis stage, apical components-containing vesicles are recruited around the midbody during the first cell division to form the AMIS, ...

Cytokinesis acceleration as a new mechanism to alter the apical midbody position in the epithelium

We discovered the new mechanism by studying the cancer promoting phosphatase of regenerating liver -3 (PRL-3).18,19 It is a dual specificity phosphatase (DSP) and belongs to the family of the PRLs (PRL-1, -2 and -3). All of them are implicated in promoting cancer progression.19 Few protein and non-protein PRL-3 substrates have been suggested, but none is thoroughly established yet.19 PRL-3 is known to promote epithelial tumor progression and metastasis but the molecular mechanism behind this clinical outcome had not been investigated before in the context of epithelial architecture and cell polarity.19 Therefore, we recently studied if PRL-3 activity would induce a cancer-relevant phenotype in polarized epithelial cells using MDCK, Caco-2 and MCF-7 epithelial cell 3D models.18 Indeed, we showed that MDCK and Caco-2 cysts overexpressing the wild type version of PRL-3 were frequently abnormal with fully polarized multiple lumens compared with single central lumen in parental MDCK and Caco-2 cysts while the cell polarity was not disrupted. Moreover, we corroborated this finding in MCF-7 3D structures. The branch ends of these complex structures contained multiple lumens when PRL-3 was present in parental MCF-7 cells, but showed single-lumen containing branch ends upon PRL-3 knockdown.18 PRL-3 substrate candidates related to epithelial polarity like ezrin or phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) were analyzed to uncover the molecular mechanism behind this observed phenotype, but no change in the phosphorylation status or localization was observed. These conclusions were drawn upon observation of static fixed confocal pictures of the 3D cysts, and therefore phosphorylation variation during dynamic processes were not possible to monitor due to physical and technical challenges impossible to solve with the state-of-art technology. Therefore, improvements in 3D cyst image acquisition would be required to further investigate the PRL-3 phenotype in this context.

Further investigation on the cellular process that underlies the ectopic lumen formation upon PRL-3 aberrant expression showed that they arise from mispositioned midbodies. However, PRL-3 did not disrupt the spindle orientation and asymmetric furrow ingression, the two already postulated mechanisms,8 suggesting that a third alternative mechanism might exist.18 Since cytokinesis is fundamental for midbody positioning and PRL-3 was enriched in the cytokinetic bridge, we studied the role of PRL-3 in this cellular process.18 Indeed, we showed that PRL-3 expression accelerates abscission timing.18 Therefore, since the midbody is a structure derived from cell division, and spatial disturbance of cytokinesis leads to midbody mispositioning in the epithelial context, a temporal alteration of the abscission would likely infer also an alteration in the final position of the midbody. For that reason, we proposed a third scenario where the acceleration of cytokinesis timing by PRL-3 might be the cause of the ectopic lumen phenotype observed in 3D epithelial cysts (Fig. 1, process labeled ‘3’). Since it is highly challenging with the currently available technologies to follow the apical or lateral positioning of the midbody by life-cell imaging in the 3D cysts, an alternative mechanism where the midbody reaches the apical membrane after cytokinesis and then it is located back in the lateral membrane by the action of PRL-3 cannot be excluded. However, this is an unlikely scenario given that PRL-3 does not influence cell polarity itself,18 that it is enriched in the cytokinetic bridge,18 and that cytokinesis timing and midbody positioning are intertwined.13,18

Future work on PRL-3's role in cytokinesis promises to reveal the molecular mechanism behind this phenotype. Proposed PRL-3 substrates like PI(4,5)P2 or ezrin, which where initially not studied in the context of cytokinesis,18 or PRL-3 interacting proteins like CNNM magnesium transporters,20,21 of which CNNM4 does not co-localize with PRL-3 in MDCK cysts during interphase (own unpublished data), can still play a role in PRL-3's effect on cytokinesis and with that in the disruption of epithelial architecture. Nevertheless, studying cytokinesis in 3D polarized cells remains a challenge as these systems cannot be synchronized, and the event of cytokinesis is rather rare and difficult to detect in statistically relevant numbers using microscopy techniques.

The post-mitotic midbody might act as a signal unit in for apical specificity

Midbody remnants can participate in non-cytokinetic functions.17,22,23 Accordingly, our results confirm that midbodies have essential functions in de novo lumenogenesis and lumen growth during cytokinesis and right after abscission,8,12,16,18 but their fate beyond that time line remained previously unexplored. In the process of our published research, we realized that midbody remnants persist long after lumen opening where they delineate the edge of the central and also ectopic lumens instead of being degraded,18 suggesting a role long after cell division in polarity maintenance or specification. One hypothesis, based on the midbody being the center for the delivery of apical components,11-13 is that the midbody might support the stability or maintenance of apical polarity by inferring apical polarity to membranes in polarized systems.

Here, we wanted to further investigate this hypothesis. To this end, we delivered midbody remnants purified from non-polarized 2-dimensional (2D) culture (Fig. 2A,,BB),24 to the top (apical membrane) or bottom (basal membrane) side of a 2D polarized monolayer of MDCK cells. Ectopic midbodies delivered to the bottom induced a partial shift of the localization of apical (actin and ζPKC) and basal (integrin-α6) markers 24 hours after being delivered (Fig. 2C-G). However, other polarity markers like ZO-118or the cis-Golgi matrix protein GM13025 did not show any changes in localization (Fig. 2H,,I)I) suggesting a selective shift of polarity.26 As control we treated the cells in the same manner with samples obtained through the same purification procedure using non-synchronized cells, which did not contain midbodies (Fig. 2A,,B).B). Cells treated in this manner did not show a shift of polarity markers (Fig. 2C-I), showing the dependence on the presence of the midbodies for the polarity shift. To discard the possibility that the actin shift could be due to an engulfment of the midbody in a phagocytotic manner,27 ectopic midbodies purified from YFP-MKLP-1 overexpressing MDCK cells (Fig. 2J) were added on top or to the bottom of MDCK 2D polarized cell cultures. Since we observed no engulfment of the YFP-tagged midbodies (Fig. 2K), we confirmed that the observed actin enrichment is not due to phagocytosis.

Figure 2.
The post-mitotic midbody infers apical specificity to basal membranes. (A) Representative confocal images of midbodies purified from synchronized cells (+ nocodazole) spinned down over poly-l-lysine coverslips and stained with Cep-55 (magenta), MKLP-1 ...

Taken together, these results indicate that midbodies might act as a signal unit containing apical specifiers needed for apical polarity, suggesting an active role of the post-mitotic midbody in cell polarity. This signal unit seems to be beneficial or required for apical stability, as the midbodies are not degraded over time.18 However, other factors are important as well since the apical-basal axis orientation is not completely shifted by the midbodies in a pre-established polarized structure.

Conclusion

In our previous study, which we have reviewed and discussed here, we suggested a novel mechanism for the loss of epithelial organization in cancer where cytokinesis timing might be involved in causing the misplacement of midbody remnants without altering the mitotic spindle orientation or asymmetric abscission. We identified PRL-3 as an enzyme involved in this process. Moreover, in the process of our research, we also observed that midbody remnants stay on the edge of the lumen, indicating a role in polarity maintenance. Here, we cast light on the fate of midbody remnants after cytokinesis suggesting that they might act as polarity signal unit for apical membrane specificity. This short communication is a step toward proving this hypothesis, and further work is required to address how PRL-3 affects cytokinesis timing, if this temporal disruption is undoubtedly the direct cause of midbody misplacement, and what the components within the midbody are that allow for apical specification. A large part of these studies will however require advancements in the suitable technology.

Methods

2D MDCK polarized cultures

4 × 104 MDCK cells were plated on Transwell filters (12 mm, 0.4 μm pore size; Corning CoStar) and grown in Minimum Essential Medium (MEM) containing 10% fetal bovine serum (FBS), (both from Gibco, Life Technologies), 100 U/mL penicillin and 100 μg/mL streptomycin (Sigma) and 2 mM L-glutamine (Sigma) for 3 d before the incubation with purified midbodies over 24 h.

Antibodies and immunolabelling

Primary mouse antibodies used were anti-γ-Tubulin (1:100; Sigma, clone GTU-88) and anti-GM130 (1:100; BD Bioscience). Primary rabbit antibodies used were anti-MKLP1 (1:1000, Santa Cruz Biotechnology, clone N-19), anti-ZO-1 (1:200; Invitrogen) and anti-PKCζ (1:200;Santa Cruz Biotechnology). Primary rat antibody used was anti-integrin α6 (1:100; Millipore). Primary goat antibody used was anti-Cep55 (1:100; Santa Cruz Biotechnology). Alexa fluorophore-conjugated secondary antibodies (1:1000 for all secondary antibodies; Invitrogen) or rhodamine-phalloidin (1:1000; Invitrogen) and Hoechst to label nuclei (10 μg/mL) were used. The procedure for the immunofluorescence staining and image data acquisition and analysis was described previously in detail.18

Isolation of midbodies24

Monolayer cultures of MDCK cells or MDCK stable expressing YFP-MKLP1 were synchronized in metaphase by adding nocodazole (Sigma) to a final concentration of 100 ng/mL for 16 h. Mitotically-arrested cells were harvested by shaking the culture flask. Cells were washed and resuspended with fresh medium and incubate at 37°C with gentle shaking for 25 min. Cells were subsequently pelleted at 200 g for 3 min and gently resuspended in 25 volumes of a hypotonic solution consisting of 1M hexylene glycol (2-methyl-2,4-pentanediol), 20 μM MgCl2, and 2 mM piperazine-N-N'-bis(2-ethane sulfonic acid) (PIPES), pH 7.2, at room temperature. Cells were immediately pelleted at 200 × g for 3 min and vigorously resuspended in 50 volumes of a lysing solution consisting of 1 M hexylene glycol, 1 mM EGTA, 1% Nonidet P-40, 2 mM PIPES, pH 7.2, at 37°C. Disruption of the cells and release of midbodies were completed by vigorous vortexing for 1 min in this solution. Midbodies were then stabilized by chilling on ice and by adding to the lysate 0.3 volume of cold 1M hexylene glycol, 50 mM 2-(N-morpholino) ethane sulfonic acid (MES), pH 6.3, to lower the pH. After centrifugation at 250 × g for 10 min to remove large debris, the supernatant was layered over a cushion of 40% glycerol (w/v) in 50 mM MES, pH 6.3, and centrifuged for 40 min at 2800 × g. This pellet was resuspended in 50 mM MES, pH 6.3, and centrifuged again through 40% glycerol and subsequently resuspended in MES buffer. Finally, the pellet obtained from a 10 cm culture dish untreated (no midbodies) or treated with nocodazole (with midbodies) was washed twice with fresh MDCK growth medium, and added to the 2D polarized live cell culture resuspended in cell medium. The controls were treated exactly the same, except the cells were not synchronized. Midbody purification was confirmed by SDS-PAGE and immunobloting against MKLP1 and γ–tubulin. The whole pellet obtained from a 10 cm culture dish untreated (no midbodies) or treated with nocodazole (with midbodies) was loaded in the acrylamide gel. Moreover, 250 μL of purified midbodies were spinned down at 4°C at 2000 g during 20 min in a 24-well for image acquisition.

Data analysis

Ordinary 2-tailed one-way ANOVA and Tukey's multiple comparison test were performed to study statistical significance: (***) p ≤ 0.001 and (****) p ≤ 0.0001. Statistical analysis and graphs were performed with Prism (GraphPad).

Disclosure of potential conflicts of interest

No potential conflicts of interest were disclosed.

Acknowledgments

We thank Martin Jechlinger for discussions for providing apico-basal polarity markers. We thank Pierre Neveu for discussions. We thank the Advanced Light Microscopy Facility at EMBL Heidelberg for support.

Funding

T.R. thanks EMBL and Marie Curie Actions for the EMBL Interdisciplinary Postdoc (EIPOD) fellowship. P.L. and G.V. thank the EMBL International PhD Program for support.

References

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