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Logo of actae2this articlesearchopen accesssubmitActa Crystallographica Section E: Crystallographic CommunicationsActa Crystallographica Section E: Crystallographic Communications
 
Acta Crystallogr E Crystallogr Commun. 2017 September 1; 73(Pt 9): 1368–1371.
Published online 2017 August 21. doi:  10.1107/S2056989017011902
PMCID: PMC5588582

Crystal structures of hibiscus acid and hibiscus acid dimethyl ester isolated from Hibiscus sabdariffa (Malvaceae)

Abstract

The biologically active title compounds have been isolated from Hibiscus sabdariffa plants, hibiscus acid as a dimethyl sulfoxide monosolvate [systematic name: (2S,3R)-3-hy­droxy-5-oxo-2,3,4,5-tetra­hydro­furan-2,3-di­carb­oxy­lic acid dimethyl sulfoxide monosolvate], C6H6O7·C2H6OS, (I), and hibiscus acid dimethyl ester [systematic name: dimethyl (2S,3R)-3-hy­droxy-5-oxo-2,3,4,5-tetra­hydro­furan-2,3-di­carboxyl­ate], C8H10O7, (II). Compound (I) forms a layered structure with alternating layers of lactone and solvent mol­ecules, that include a two-dimensional hydrogen-bonding construct. Compound (II) has two crystallographically independent and conformationally similar mol­ecules per asymmetric unit and forms a one-dimensional hydrogen-bonding construct. The known absolute configuration for both compounds has been confirmed.

Keywords: crystal structure, natural products, hibiscus, lactone acids, hydrogen bonding

Chemical context  

Lactone acid producing plants, including Hibiscus sabdariffa (Malvaceae), have been documented to have significant potential in the traditional treatment of various diseases. H. sabdariffa Linn is a species of hibiscus from the Malvaceae family, commonly known as ‘Karkade’ or ‘red sorrel’. It is used in traditional medicine in the form of herbal teas or cold drinks for its hypotensive and diuretic effects and to lower body temperature and blood viscosity (Ali et al., 2005  ; Da-Costa-Rocha et al., 2014  ). Little attention has been paid to organic acids from H. sabdariffa, specifically hibiscus acid. However, studies have documented the activity of hibiscus acid and hibiscus acid methyl ester. These report an inhibitory effect against enzymes, such as α-amylase and α-glucosidase (Hansawasdi et al., 2000  , 2001  ). As these compounds are not available commercially and to enable a study of their biological activities, we report on the extraction of hibiscus acid and hibiscus acid dimethyl ester from H. sabdariffa (Malvaceae), and on their purification and characterization. The crystal structures of the acid, as the dimethyl sulfoxide monosolvate, (I), and the diester, (II), are reported herein.

Structural commentary  

The crystal structures of the 1:1 dimethyl sulfoxide (DMSO) solvate of hibiscus acid, (I), and of hibiscus acid dimethyl ester, (II), are shown in Figs. 1  and 2  . The COOR (R = H or Me) groups lie in equatorial positions on their rings and the absolute configuration of both species is confirmed by the Flack parameter values (Parsons et al., 2013  ), for arbitrarily named atoms in (I) [C2(R),C1(S), 0.00 (4)] and both arbitrarily named equivalent atoms in (II) [C3(R),C4(S) and C11(R),C12(S), 0.08 (17)] (Table 1  ). The absolute configuration found thus agrees with that originally proposed by Boll et al. (1969  ) for hibiscus acid. The structure of garcinia lactone, an epimer of hibiscus acid, has been reported (Mahapatra et al., 2007  ). The comparable mol­ecular geometries of (I) and its epimer are similar. The five-membered ring of (I) adopts an envelope conformation, with the OH-bearing C2 atom 0.582 (6) Å out of the plane defined by the other four atoms.

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Object name is e-73-01368-scheme1.jpg

Figure 1
The mol­ecular structure of compound (I), with the atom labelling and 50% probability displacement ellipsoids.
Figure 2
The mol­ecular structures of the two independent mol­ecules comprising the asymmetric unit of (II), with the atom labelling and 50% probability displacement ellipsoids.
Table 1
Experimental details

The structure of (II) contains two crystallographically independent mol­ecules (A and B) (Z′ = 2), whose mol­ecular geometries differ only by small deviations in torsion angles, for example, C3—C5—O5—C6 in A is 175.1 (4)°, whilst the equivalent angle in B (C11—C13—O12—C—14) is 180.0 (4)°. As with structure (I), the five-membered rings adopt envelope conformations, with the OH-bearing C atoms lying out of the plane of the other four atoms, here by 0.505 (5) and 0.530 (5) Å for mol­ecules A and B, respectively.

Supra­molecular features  

Despite containing two carb­oxy­lic acid functionalities, the structure of (I) does not feature the classic An external file that holds a picture, illustration, etc.
Object name is e-73-01368-efi1.jpg(8) carb­oxy­lic acid dimer motif. Instead, each of the three potential hydrogen-bond donors of the acid mol­ecule form inter­actions with a total of three separate neighbouring mol­ecules (Fig. 3  ). The H atom of the carb­oxy­lic acid group (O3—H) adjacent to the ether forms a bifurcated hydrogen bond that is accepted by the ROH and C=O functions (i.e. O4i and O6i) of one neighbour, whilst the other two donors, the second carb­oxy­lic acid (O5—H) and the hy­droxy group (O4—H), form hydrogen bonds with atoms O8ii and O8 of DMSO solvent mol­ecules, respectively (Table 2  ). These inter­actions combine to give a two-dimensional hydrogen-bonded layered structure, with DMSO and acid layers alternating along the c-cell direction (Fig. 4  ).

Figure 3
Hydrogen-bonding contacts in (I).
Figure 4
The crystal packing of compound (I), viewed along the a axis.
Table 2
Hydrogen-bond geometry (Å, °) for (I)

Both independent mol­ecules in the structure of (II) donate single hydrogen bonds through their OH groups, but only one mol­ecule (A) acts as a hydrogen-bond acceptor (O3—H(...)O4i and O10—H(...)O2ii; Table 3  ). That a total of four carbonyl O atoms do not act as acceptors is probably related to the low ratio of classic hydrogen-bond donors to acceptors in this compound. In (II), the hydrogen bonding combines to give a four-mol­ecule-wide one-dimensional ribbon of linked mol­ecules that propagates parallel to the a axis (Fig. 5  ).

Figure 5
A section of the extended structure of (II), with the hydrogen-bonded polymer extending left and right parallel to the a axis.
Table 3
Hydrogen-bond geometry (Å, °) for (II)

Database survey  

A search of the Cambridge Structural Database (Version 5.37, searched June 2017; Groom et al., 2016  ) yielded few relevant structures. For hibiscus acid, only the structures of a Ca salt form (Glusker et al., 1972  ) and of the diastereomer mentioned previously (Mahapatra et al., 2007  ) have been reported. The closest relative of (II) to have been structurally described is a derivative with additional OH and Me substituents on the five-membered ring (Evans et al., 1997  ).

Synthesis and crystallization  

Dried H. sabdariffa calyces were crushed to a powder (500 g) and extracted in a Soxhlet apparatus using 2500 ml each of hexane, ethyl acetate and methanol. The methanol extract was dried and concentrated at 313 K by rotatory evaporation, yielding about 125 g (25%) of crude extract. The methanol extract (2 g) was dissolved in about 2 ml of methanol and subjected to gel filtration chromatography (GFC) using a glass column packed with a wet slurry of 30 g of Sephadex LH20 in methanol. Vials were collected (5 ml each) after elution with 100% methanol, which led to isolation of pure hibiscus acid (0.5%). Crystals of (I) were obtained by recrystallisation from DMSO. For nonsolvated material, 1H NMR [OC(CD3)2]: 5.31 (1H, s), 3.23 (1H, d, J = 17.19 Hz), 2.77 (1H, d, J = 17.18 Hz). HRMS: found 189.0000; calculated 189.0035.

Hibiscus acid dimethyl ester, (II), was obtained from the methanol extract (20 g) using vacuum liquid chromatography (VLC) eluted with solvent systems in different ratios to increase the polarity. The ethyl acetate portion was evaporated and a thick paste was obtained. A pure precipitate of the compound (5%) was obtained by addition of propan-2-ol to the dried ethyl acetate fraction. 1H NMR [OC(CD3)2]: 5.35 (1H, s), 3.23 (1H, d, J = 17.28 Hz), 2.77 (1H, d, J = 17.31 Hz), 3.87 (3H, s), 3.76 (3H, s). HRMS: found 218.000; calculated 218.035.

Refinement  

Crystal data, data collection and structure refinement details are summarized in Table 1  . For all structures, C-bound H atoms were placed in their expected geometrical positions and treated as riding, with C—H = 0.95–0.99 Å and U iso(H) = 1.5U eq(C) for methyl C atoms and 1.2U eq(C) for the other H atoms. The absolute configuraion was determined for the mol­ecules in both acid (I) for arbitrarily named atoms [C2(R),C1(S), Flack parameter 0.00 (4)] and both arbitrarily named equivalent atoms in (II) [C3(R),C4(S) (mol­ecule A) and C11(R),C12(S) (mol­ecule B), Flack parameter 0.08 (17)] (Parsons et al., 2013  ).

Supplementary Material

Crystal structure: contains datablock(s) I, II, global. DOI: 10.1107/S2056989017011902/zs2386sup1.cif

Structure factors: contains datablock(s) I. DOI: 10.1107/S2056989017011902/zs2386Isup2.hkl

Structure factors: contains datablock(s) II. DOI: 10.1107/S2056989017011902/zs2386IIsup3.hkl

CCDC references: 1569231, 1569230

Additional supporting information: crystallographic information; 3D view; checkCIF report

Acknowledgments

We thank the College of Pharmacy, University of Misan, and the Ministry of Higher Education, Iraq, for funding AZ.

supplementary crystallographic information

(2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylic acid dimethyl sulfoxide monosolvate (I)   Crystal data

C6H6O7·C2H6OSF(000) = 280
Mr = 268.24Dx = 1.608 Mg m3
Monoclinic, P21Cu Kα radiation, λ = 1.5418 Å
a = 5.4258 (2) ÅCell parameters from 2057 reflections
b = 8.9491 (3) Åθ = 6.3–72.8°
c = 11.4365 (3) ŵ = 2.94 mm1
β = 94.092 (3)°T = 123 K
V = 553.90 (3) Å3Fragment from a square plate, colourless
Z = 20.30 × 0.15 × 0.05 mm

(2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylic acid dimethyl sulfoxide monosolvate (I)   Data collection

Oxford Diffraction Gemini S CCD diffractometer1640 reflections with I > 2σ(I)
Radiation source: sealed tubeRint = 0.054
ω scansθmax = 72.8°, θmin = 3.9°
Absorption correction: multi-scan (CrysAlis PRO; Oxford Diffraction, 2010)h = −6→6
Tmin = 0.554, Tmax = 1.000k = −10→8
4397 measured reflectionsl = −14→14
1854 independent reflections

(2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylic acid dimethyl sulfoxide monosolvate (I)   Refinement

Refinement on F2Hydrogen site location: mixed
Least-squares matrix: fullH atoms treated by a mixture of independent and constrained refinement
R[F2 > 2σ(F2)] = 0.047w = 1/[σ2(Fo2) + (0.0678P)2] where P = (Fo2 + 2Fc2)/3
wR(F2) = 0.113(Δ/σ)max < 0.001
S = 1.05Δρmax = 0.44 e Å3
1854 reflectionsΔρmin = −0.25 e Å3
169 parametersAbsolute structure: Flack x determined using 698 quotients [(I+)-(I-)]/[(I+)+(I-)] (Parsons et al., 2013)
4 restraintsAbsolute structure parameter: 0.00 (4)

(2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylic acid dimethyl sulfoxide monosolvate (I)   Special details

Geometry. All esds (except the esd in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell esds are taken into account individually in the estimation of esds in distances, angles and torsion angles; correlations between esds in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell esds is used for estimating esds involving l.s. planes.
Refinement. Refined as a 2-component inversion twin

(2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylic acid dimethyl sulfoxide monosolvate (I)   Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å2)

xyzUiso*/Ueq
S10.90564 (19)1.26444 (16)0.85370 (9)0.0235 (3)
O10.6239 (6)0.7220 (4)0.8066 (3)0.0233 (9)
O21.0711 (6)0.6125 (5)0.7467 (3)0.0285 (9)
O30.9028 (6)0.5501 (5)0.5682 (3)0.0276 (8)
O40.8575 (6)0.9112 (5)0.6333 (3)0.0232 (8)
O50.3551 (6)0.7366 (5)0.4714 (3)0.0265 (9)
O60.6572 (6)0.8844 (5)0.4153 (3)0.0254 (8)
O70.4156 (6)0.8927 (5)0.9015 (3)0.0300 (9)
O80.8239 (7)1.1798 (5)0.7411 (3)0.0295 (9)
C10.6534 (8)0.6840 (7)0.6856 (4)0.0231 (11)
H10.51850.61490.65570.028*
C20.6288 (8)0.8370 (6)0.6206 (4)0.0219 (11)
C30.4303 (8)0.9097 (7)0.6897 (4)0.0236 (11)
H3A0.44661.01980.69010.028*
H3B0.26270.88230.65670.028*
C40.4814 (8)0.8461 (7)0.8109 (4)0.0244 (11)
C50.9026 (8)0.6125 (7)0.6737 (4)0.0221 (11)
C60.5504 (8)0.8209 (6)0.4898 (4)0.0216 (10)
C71.2345 (8)1.2784 (8)0.8549 (4)0.0273 (12)
H7A1.27881.34170.78970.041*
H7B1.30531.17860.84650.041*
H7C1.29971.32270.92920.041*
C80.8906 (10)1.1285 (8)0.9679 (4)0.0309 (13)
H8A0.98981.04110.94980.046*
H8B0.71841.09810.97390.046*
H8C0.95521.17201.04250.046*
H2H0.308 (11)0.729 (8)0.396 (3)0.032 (17)*
H1H1.043 (7)0.509 (7)0.555 (5)0.026 (15)*
H3H0.865 (17)0.987 (7)0.682 (6)0.07 (3)*

(2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylic acid dimethyl sulfoxide monosolvate (I)   Atomic displacement parameters (Å2)

U11U22U33U12U13U23
S10.0241 (5)0.0207 (7)0.0254 (5)0.0013 (5)0.0002 (4)−0.0021 (5)
O10.0228 (14)0.024 (3)0.0232 (15)0.0022 (13)0.0016 (11)0.0007 (13)
O20.0237 (16)0.030 (3)0.0320 (17)0.0050 (15)0.0000 (13)0.0007 (16)
O30.0213 (14)0.032 (3)0.0299 (16)0.0032 (15)0.0023 (12)−0.0049 (16)
O40.0187 (14)0.021 (2)0.0299 (17)−0.0025 (14)0.0029 (12)−0.0026 (15)
O50.0248 (14)0.030 (3)0.0243 (14)−0.0046 (15)−0.0009 (11)0.0008 (14)
O60.0258 (15)0.024 (2)0.0267 (15)−0.0024 (15)0.0046 (12)0.0010 (15)
O70.0308 (16)0.033 (3)0.0274 (17)0.0032 (16)0.0073 (13)−0.0037 (17)
O80.0329 (18)0.025 (3)0.0296 (17)0.0006 (17)−0.0058 (14)0.0030 (17)
C10.019 (2)0.027 (3)0.024 (2)0.000 (2)0.0022 (16)−0.001 (2)
C20.0178 (19)0.018 (3)0.030 (2)−0.0008 (19)0.0023 (16)0.001 (2)
C30.0184 (19)0.023 (3)0.030 (2)−0.0014 (19)0.0027 (16)−0.003 (2)
C40.019 (2)0.024 (3)0.030 (2)−0.0040 (19)0.0025 (16)−0.002 (2)
C50.023 (2)0.017 (3)0.028 (2)0.0014 (19)0.0073 (18)0.005 (2)
C60.019 (2)0.019 (3)0.027 (2)0.0031 (18)0.0028 (16)−0.0004 (19)
C70.0204 (18)0.031 (4)0.030 (2)−0.001 (2)0.0012 (16)0.001 (2)
C80.032 (2)0.033 (4)0.027 (2)−0.004 (2)0.0027 (18)0.006 (2)

(2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylic acid dimethyl sulfoxide monosolvate (I)   Geometric parameters (Å, º)

S1—O81.532 (4)C1—C51.511 (6)
S1—C71.788 (5)C1—C21.559 (8)
S1—C81.791 (6)C1—H11.0000
O1—C41.356 (7)C2—C31.525 (6)
O1—C11.445 (6)C2—C61.532 (6)
O2—C51.194 (6)C3—C41.505 (7)
O3—C51.329 (6)C3—H3A0.9900
O3—H1H0.87 (3)C3—H3B0.9900
O4—C21.406 (6)C7—H7A0.9800
O4—H3H0.87 (3)C7—H7B0.9800
O5—C61.306 (6)C7—H7C0.9800
O5—H2H0.89 (3)C8—H8A0.9800
O6—C61.206 (6)C8—H8B0.9800
O7—C41.195 (6)C8—H8C0.9800
O8—S1—C7105.7 (2)C2—C3—H3B111.2
O8—S1—C8104.6 (3)H3A—C3—H3B109.1
C7—S1—C898.0 (3)O7—C4—O1121.5 (5)
C4—O1—C1109.3 (4)O7—C4—C3128.3 (5)
C5—O3—H1H113 (4)O1—C4—C3110.1 (4)
C2—O4—H3H116 (6)O2—C5—O3125.8 (5)
C6—O5—H2H112 (4)O2—C5—C1125.6 (5)
O1—C1—C5110.3 (4)O3—C5—C1108.6 (4)
O1—C1—C2103.8 (4)O6—C6—O5125.7 (4)
C5—C1—C2112.1 (4)O6—C6—C2122.1 (5)
O1—C1—H1110.2O5—C6—C2112.1 (4)
C5—C1—H1110.2S1—C7—H7A109.5
C2—C1—H1110.2S1—C7—H7B109.5
O4—C2—C3113.3 (4)H7A—C7—H7B109.5
O4—C2—C6109.0 (4)S1—C7—H7C109.5
C3—C2—C6112.9 (4)H7A—C7—H7C109.5
O4—C2—C1108.7 (4)H7B—C7—H7C109.5
C3—C2—C199.6 (4)S1—C8—H8A109.5
C6—C2—C1113.0 (5)S1—C8—H8B109.5
C4—C3—C2103.0 (4)H8A—C8—H8B109.5
C4—C3—H3A111.2S1—C8—H8C109.5
C2—C3—H3A111.2H8A—C8—H8C109.5
C4—C3—H3B111.2H8B—C8—H8C109.5
C4—O1—C1—C5148.2 (4)C2—C3—C4—O7161.1 (5)
C4—O1—C1—C227.9 (5)C2—C3—C4—O1−17.9 (5)
O1—C1—C2—O482.0 (4)O1—C1—C5—O2−13.6 (8)
C5—C1—C2—O4−37.1 (5)C2—C1—C5—O2101.5 (6)
O1—C1—C2—C3−36.8 (4)O1—C1—C5—O3166.6 (4)
C5—C1—C2—C3−155.9 (4)C2—C1—C5—O3−78.3 (6)
O1—C1—C2—C6−156.8 (3)O4—C2—C6—O6−10.2 (7)
C5—C1—C2—C684.1 (5)C3—C2—C6—O6116.7 (5)
O4—C2—C3—C4−83.0 (5)C1—C2—C6—O6−131.2 (5)
C6—C2—C3—C4152.4 (5)O4—C2—C6—O5172.0 (4)
C1—C2—C3—C432.3 (5)C3—C2—C6—O5−61.2 (6)
C1—O1—C4—O7174.3 (5)C1—C2—C6—O550.9 (5)
C1—O1—C4—C3−6.6 (5)

(2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylic acid dimethyl sulfoxide monosolvate (I)   Hydrogen-bond geometry (Å, º)

D—H···AD—HH···AD···AD—H···A
O3—H1H···O4i0.87 (2)2.42 (4)2.996 (4)124 (3)
O3—H1H···O6i0.87 (2)1.98 (3)2.805 (4)158 (4)
O4—H3H···O80.87 (2)1.87 (3)2.714 (5)160 (7)
O5—H2H···O8ii0.89 (2)1.73 (2)2.603 (4)167 (5)

Symmetry codes: (i) −x+2, y−1/2, −z+1; (ii) −x+1, y−1/2, −z+1.

Dimethyl (2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylate (II)   Crystal data

C8H10O7F(000) = 456
Mr = 218.16Dx = 1.519 Mg m3
Monoclinic, P21Cu Kα radiation, λ = 1.5418 Å
a = 9.3057 (6) ÅCell parameters from 3289 reflections
b = 7.6934 (6) Åθ = 3.4–72.8°
c = 13.4012 (11) ŵ = 1.20 mm1
β = 96.243 (7)°T = 123 K
V = 953.74 (12) Å3Platey fragment, colourless
Z = 40.30 × 0.20 × 0.04 mm

Dimethyl (2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylate (II)   Data collection

Oxford Diffraction Gemini S CCD diffractometer2976 reflections with I > 2σ(I)
Radiation source: sealed tubeRint = 0.036
ω scansθmax = 73.4°, θmin = 3.3°
Absorption correction: multi-scan (CrysAlis PRO; Oxford Diffraction, 2010)h = −11→11
Tmin = 0.747, Tmax = 1.000k = −8→9
8046 measured reflectionsl = −16→14
3506 independent reflections

Dimethyl (2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylate (II)   Refinement

Refinement on F2Hydrogen site location: mixed
Least-squares matrix: fullH atoms treated by a mixture of independent and constrained refinement
R[F2 > 2σ(F2)] = 0.044w = 1/[σ2(Fo2) + (0.0568P)2 + 0.1462P] where P = (Fo2 + 2Fc2)/3
wR(F2) = 0.121(Δ/σ)max < 0.001
S = 1.10Δρmax = 0.23 e Å3
3506 reflectionsΔρmin = −0.22 e Å3
281 parametersAbsolute structure: Flack x determined using 1098 quotients [(I+)-(I-)]/[(I+)+(I-)] (Parsons et al., 2013)
3 restraintsAbsolute structure parameter: 0.08 (17)

Dimethyl (2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylate (II)   Special details

Geometry. All esds (except the esd in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell esds are taken into account individually in the estimation of esds in distances, angles and torsion angles; correlations between esds in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell esds is used for estimating esds involving l.s. planes.

Dimethyl (2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylate (II)   Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å2)

xyzUiso*/Ueq
O10.0127 (3)0.3894 (4)0.4143 (2)0.0393 (7)
O2−0.0438 (4)0.4647 (5)0.2547 (2)0.0502 (8)
O30.3364 (3)0.3776 (4)0.4119 (2)0.0399 (7)
H1H0.407 (4)0.349 (7)0.458 (3)0.048*
O40.4351 (3)0.0487 (4)0.4527 (2)0.0460 (8)
O50.2148 (3)−0.0633 (4)0.4061 (2)0.0432 (7)
O60.1333 (3)0.5084 (4)0.5929 (2)0.0460 (7)
O70.2626 (3)0.2625 (4)0.6205 (2)0.0431 (7)
O80.5725 (3)0.4161 (4)−0.0594 (2)0.0391 (7)
O90.6406 (3)0.5063 (5)−0.2045 (2)0.0484 (8)
O100.8715 (3)0.4023 (4)0.0508 (2)0.0388 (7)
H2H0.904 (5)0.376 (7)0.1128 (16)0.047*
O110.9307 (3)0.0730 (4)0.1173 (2)0.0437 (7)
O120.7678 (3)−0.0374 (4)−0.0028 (3)0.0467 (8)
O130.5379 (3)0.5087 (4)0.1312 (2)0.0443 (7)
O140.6548 (3)0.2682 (4)0.1911 (2)0.0414 (7)
C10.0312 (5)0.3802 (6)0.3161 (3)0.0408 (9)
C20.1501 (5)0.2555 (7)0.3003 (3)0.0424 (10)
H2A0.21160.30120.25050.051*
H2B0.11060.14120.27710.051*
C30.2350 (4)0.2408 (6)0.4034 (3)0.0366 (9)
C40.1125 (4)0.2751 (6)0.4721 (3)0.0377 (9)
H40.06370.16380.48700.045*
C50.3083 (5)0.0651 (6)0.4238 (3)0.0389 (9)
C60.2722 (5)−0.2380 (6)0.4145 (4)0.0477 (11)
H6A0.3464−0.25210.36850.072*
H6B0.1940−0.32160.39710.072*
H6C0.3151−0.25870.48350.072*
C70.1678 (4)0.3672 (6)0.5685 (3)0.0378 (9)
C80.3289 (6)0.3351 (8)0.7144 (4)0.0564 (13)
H8A0.39600.42790.70030.085*
H8B0.38190.24370.75380.085*
H8C0.25370.38260.75240.085*
C90.6617 (5)0.4150 (6)−0.1329 (3)0.0393 (9)
C100.7821 (4)0.2878 (6)−0.1067 (3)0.0396 (9)
H10A0.87500.3340−0.12520.048*
H10B0.76160.1755−0.14140.048*
C110.7864 (4)0.2667 (6)0.0067 (3)0.0352 (9)
C120.6230 (4)0.2963 (6)0.0191 (3)0.0358 (9)
H120.56940.18400.00900.043*
C130.8388 (4)0.0905 (6)0.0475 (3)0.0370 (9)
C140.8023 (5)−0.2154 (7)0.0250 (4)0.0478 (11)
H14A0.8702−0.2623−0.01920.072*
H14B0.7137−0.28510.01820.072*
H14C0.8465−0.21930.09470.072*
C150.5978 (4)0.3732 (6)0.1191 (3)0.0365 (9)
C160.6380 (6)0.3211 (7)0.2932 (4)0.0504 (12)
H16A0.68570.43340.30710.076*
H16B0.68200.23380.34020.076*
H16C0.53490.33190.30120.076*

Dimethyl (2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylate (II)   Atomic displacement parameters (Å2)

U11U22U33U12U13U23
O10.0391 (13)0.0337 (18)0.0436 (16)0.0050 (13)−0.0023 (11)−0.0018 (13)
O20.0564 (18)0.046 (2)0.0456 (17)0.0136 (15)−0.0086 (14)−0.0029 (14)
O30.0377 (14)0.0332 (18)0.0481 (16)−0.0031 (13)0.0012 (12)0.0019 (13)
O40.0439 (16)0.040 (2)0.0519 (17)0.0044 (14)−0.0055 (13)−0.0016 (13)
O50.0441 (16)0.0288 (17)0.0561 (18)−0.0004 (13)0.0023 (13)0.0014 (13)
O60.0513 (16)0.0371 (19)0.0477 (17)0.0081 (14)−0.0031 (14)−0.0090 (14)
O70.0528 (16)0.0314 (18)0.0429 (16)0.0050 (14)−0.0048 (13)0.0029 (13)
O80.0386 (14)0.0344 (18)0.0431 (15)0.0009 (12)−0.0014 (11)0.0040 (12)
O90.0510 (17)0.049 (2)0.0441 (17)−0.0017 (15)0.0000 (14)0.0089 (14)
O100.0405 (14)0.0341 (19)0.0404 (15)−0.0032 (13)−0.0021 (11)−0.0002 (12)
O110.0484 (16)0.0373 (18)0.0439 (16)0.0042 (14)−0.0015 (13)−0.0001 (13)
O120.0433 (16)0.0281 (19)0.066 (2)0.0007 (13)−0.0051 (14)−0.0063 (14)
O130.0493 (16)0.039 (2)0.0428 (16)0.0079 (14)−0.0027 (13)−0.0032 (14)
O140.0492 (15)0.0353 (18)0.0400 (15)0.0056 (14)0.0063 (12)0.0045 (13)
C10.044 (2)0.033 (3)0.043 (2)0.0020 (19)−0.0042 (17)−0.0038 (18)
C20.049 (2)0.036 (3)0.040 (2)0.0044 (19)−0.0029 (17)−0.0028 (18)
C30.041 (2)0.027 (2)0.041 (2)−0.0035 (17)0.0022 (16)−0.0005 (16)
C40.0380 (19)0.031 (2)0.043 (2)−0.0024 (17)−0.0014 (16)0.0003 (18)
C50.043 (2)0.037 (3)0.036 (2)0.0028 (18)0.0024 (16)−0.0034 (17)
C60.055 (3)0.032 (3)0.056 (3)0.002 (2)0.005 (2)0.002 (2)
C70.0351 (18)0.039 (3)0.039 (2)−0.0013 (17)0.0045 (15)0.0006 (18)
C80.069 (3)0.050 (3)0.046 (3)0.004 (2)−0.015 (2)0.002 (2)
C90.045 (2)0.036 (3)0.037 (2)−0.0065 (18)−0.0001 (16)−0.0026 (17)
C100.041 (2)0.037 (3)0.040 (2)−0.0018 (18)0.0036 (16)−0.0003 (17)
C110.0377 (19)0.029 (2)0.039 (2)0.0002 (16)0.0020 (15)−0.0029 (17)
C120.039 (2)0.026 (2)0.042 (2)−0.0024 (17)−0.0012 (16)0.0026 (17)
C130.0368 (19)0.031 (2)0.043 (2)−0.0014 (17)0.0050 (17)−0.0033 (17)
C140.042 (2)0.036 (3)0.065 (3)0.000 (2)0.007 (2)−0.004 (2)
C150.0339 (17)0.031 (2)0.044 (2)−0.0025 (17)0.0017 (15)0.0001 (17)
C160.060 (3)0.048 (3)0.043 (2)0.005 (2)0.006 (2)0.008 (2)

Dimethyl (2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylate (II)   Geometric parameters (Å, º)

O1—C11.347 (5)C2—H2B0.9900
O1—C41.442 (5)C3—C51.526 (6)
O2—C11.210 (5)C3—C41.562 (6)
O3—C31.410 (5)C4—C71.514 (6)
O3—H1H0.880 (14)C4—H41.0000
O4—C51.208 (5)C6—H6A0.9800
O5—C51.320 (5)C6—H6B0.9800
O5—C61.446 (6)C6—H6C0.9800
O6—C71.189 (6)C8—H8A0.9800
O7—C71.334 (5)C8—H8B0.9800
O7—C81.451 (6)C8—H8C0.9800
O8—C91.355 (5)C9—C101.501 (6)
O8—C121.439 (5)C10—C111.524 (6)
O9—C91.188 (5)C10—H10A0.9900
O10—C111.401 (5)C10—H10B0.9900
O10—H2H0.876 (14)C11—C131.522 (6)
O11—C131.204 (5)C11—C121.565 (5)
O12—C131.328 (5)C12—C151.506 (6)
O12—C141.446 (6)C12—H121.0000
O13—C151.202 (5)C14—H14A0.9800
O14—C151.324 (5)C14—H14B0.9800
O14—C161.452 (6)C14—H14C0.9800
C1—C21.497 (6)C16—H16A0.9800
C2—C31.519 (6)C16—H16B0.9800
C2—H2A0.9900C16—H16C0.9800
C1—O1—C4110.4 (3)H8A—C8—H8B109.5
C3—O3—H1H108 (4)O7—C8—H8C109.5
C5—O5—C6116.8 (3)H8A—C8—H8C109.5
C7—O7—C8114.6 (4)H8B—C8—H8C109.5
C9—O8—C12110.5 (3)O9—C9—O8121.5 (4)
C11—O10—H2H110 (4)O9—C9—C10128.9 (4)
C13—O12—C14119.2 (4)O8—C9—C10109.6 (4)
C15—O14—C16116.1 (4)C9—C10—C11103.9 (3)
O2—C1—O1120.7 (4)C9—C10—H10A111.0
O2—C1—C2129.0 (4)C11—C10—H10A111.0
O1—C1—C2110.3 (4)C9—C10—H10B111.0
C1—C2—C3103.7 (3)C11—C10—H10B111.0
C1—C2—H2A111.0H10A—C10—H10B109.0
C3—C2—H2A111.0O10—C11—C13111.5 (3)
C1—C2—H2B111.0O10—C11—C10107.1 (3)
C3—C2—H2B111.0C13—C11—C10115.2 (4)
H2A—C2—H2B109.0O10—C11—C12111.0 (3)
O3—C3—C2107.1 (3)C13—C11—C12111.6 (3)
O3—C3—C5111.4 (3)C10—C11—C1299.8 (3)
C2—C3—C5114.0 (4)O8—C12—C15109.2 (3)
O3—C3—C4110.6 (4)O8—C12—C11105.0 (3)
C2—C3—C4100.6 (3)C15—C12—C11113.5 (3)
C5—C3—C4112.6 (4)O8—C12—H12109.6
O1—C4—C7108.2 (3)C15—C12—H12109.6
O1—C4—C3104.8 (3)C11—C12—H12109.6
C7—C4—C3112.3 (3)O11—C13—O12125.7 (4)
O1—C4—H4110.5O11—C13—C11123.5 (4)
C7—C4—H4110.5O12—C13—C11110.8 (3)
C3—C4—H4110.5O12—C14—H14A109.5
O4—C5—O5125.5 (4)O12—C14—H14B109.5
O4—C5—C3123.5 (4)H14A—C14—H14B109.5
O5—C5—C3111.0 (3)O12—C14—H14C109.5
O5—C6—H6A109.5H14A—C14—H14C109.5
O5—C6—H6B109.5H14B—C14—H14C109.5
H6A—C6—H6B109.5O13—C15—O14125.9 (4)
O5—C6—H6C109.5O13—C15—C12125.4 (4)
H6A—C6—H6C109.5O14—C15—C12108.6 (4)
H6B—C6—H6C109.5O14—C16—H16A109.5
O6—C7—O7126.2 (4)O14—C16—H16B109.5
O6—C7—C4125.9 (4)H16A—C16—H16B109.5
O7—C7—C4107.9 (4)O14—C16—H16C109.5
O7—C8—H8A109.5H16A—C16—H16C109.5
O7—C8—H8B109.5H16B—C16—H16C109.5
C4—O1—C1—O2179.0 (4)C12—O8—C9—O9−179.1 (4)
C4—O1—C1—C2−0.5 (5)C12—O8—C9—C100.3 (5)
O2—C1—C2—C3160.9 (5)O9—C9—C10—C11158.1 (5)
O1—C1—C2—C3−19.7 (5)O8—C9—C10—C11−21.2 (5)
C1—C2—C3—O3−86.2 (4)C9—C10—C11—O10−84.8 (4)
C1—C2—C3—C5150.1 (4)C9—C10—C11—C13150.5 (4)
C1—C2—C3—C429.3 (5)C9—C10—C11—C1230.9 (4)
C1—O1—C4—C7139.9 (4)C9—O8—C12—C15142.4 (3)
C1—O1—C4—C319.9 (4)C9—O8—C12—C1120.3 (4)
O3—C3—C4—O182.7 (4)O10—C11—C12—O881.3 (4)
C2—C3—C4—O1−30.2 (4)C13—C11—C12—O8−153.6 (3)
C5—C3—C4—O1−152.0 (3)C10—C11—C12—O8−31.4 (4)
O3—C3—C4—C7−34.5 (5)O10—C11—C12—C15−37.9 (5)
C2—C3—C4—C7−147.5 (4)C13—C11—C12—C1587.1 (4)
C5—C3—C4—C790.8 (4)C10—C11—C12—C15−150.6 (4)
C6—O5—C5—O4−5.2 (6)C14—O12—C13—O11−1.2 (7)
C6—O5—C5—C3175.1 (4)C14—O12—C13—C11180.0 (4)
O3—C3—C5—O46.5 (6)O10—C11—C13—O118.2 (6)
C2—C3—C5—O4127.9 (5)C10—C11—C13—O11130.5 (4)
C4—C3—C5—O4−118.4 (5)C12—C11—C13—O11−116.6 (4)
O3—C3—C5—O5−173.8 (3)O10—C11—C13—O12−173.0 (3)
C2—C3—C5—O5−52.4 (5)C10—C11—C13—O12−50.6 (5)
C4—C3—C5—O561.3 (5)C12—C11—C13—O1262.3 (4)
C8—O7—C7—O6−1.3 (6)C16—O14—C15—O132.1 (6)
C8—O7—C7—C4178.3 (4)C16—O14—C15—C12−178.9 (4)
O1—C4—C7—O61.0 (6)O8—C12—C15—O133.7 (5)
C3—C4—C7—O6116.1 (5)C11—C12—C15—O13120.6 (5)
O1—C4—C7—O7−178.6 (3)O8—C12—C15—O14−175.2 (3)
C3—C4—C7—O7−63.4 (4)C11—C12—C15—O14−58.4 (4)

Dimethyl (2S,3R)-3-Hydroxy-5-oxo-2,3,4,5-tetrahydrofuran-2,3-dicarboxylate (II)   Hydrogen-bond geometry (Å, º)

D—H···AD—HH···AD···AD—H···A
O3—H1H···O4i0.88 (1)2.36 (5)2.951 (4)125 (4)
O10—H2H···O2ii0.88 (1)2.03 (3)2.802 (4)147 (5)

Symmetry codes: (i) −x+1, y+1/2, −z+1; (ii) x+1, y, z.

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Articles from Acta Crystallographica Section E: Crystallographic Communications are provided here courtesy of International Union of Crystallography