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Data Brief. 2017 October; 14: 629–634.
Published online 2017 August 31. doi:  10.1016/j.dib.2017.08.022
PMCID: PMC5587882

Dataset on continuous passages of Trypanosoma brucei in different laboratory animals

Abstract

Scientist in developing countries maintain trypanosomes in laboratory animals for in vivo experiments. We generated data on the adaptation of Trypanosoma brucei (NITR201 strain) in balb/c mice (forty-five, 18–23 g), wistar rats (fifteen, 180–220 g) and New Zealand white and chinchilla rabbits (fifteen, 2.8–3.0 kg) in a controlled experimental system. The weight, haematological parameters, course of parasitaemia, temperature, mean survival time and survival proportions of laboratory animals in groups A–E were collected and analysed for differences in response to the same challenge of quantity, strain and species of T. brucei. Trypanosome pleomorphism of long, intermediate to short-stumpy forms were among the dataset counts for parasitaemia. Statistical data after analysis were summarised in the supplementary file to show the differences and corresponding reaction of multiple passages.

Keywords: Trypanosoma brucei, Survival time, Laboratory animals, Virulence

Specifications Table

Table thumbnail

Value of the data

  • • The haematological data and survival time varied among different laboratory animals, and it will be useful in carefully selecting the appropriate host to maintain Trypanosoma species for any experimental design.
  • • Understanding the physiological responses of the laboratory animal hosts could suggest different human responses to Trypanosoma brucei infection.
  • • Different percentage weight gain and loss including severe pathologic responses could discourage further use of some animal models in large quantity.
  • • Differences in the physiology of individual laboratory animal could affect the pathogenicity and virulence of trypanosome organism during passages.
  • • This data allows other researchers to extend statistical analysis on experiments related to trypanosomes in the laboratory as this is becoming increasingly needful in developing countries.

1. Data

The dataset of this article provides an extensive information on physiologic and haematologic parameters associated with trypanosome continuous passaging in three laboratory animals. Analysed data on differences of the animal physiology to trypanosome is in Fig. 1, Fig. 2, Fig. 3, Fig. 4. Table 1, Table 2 show mean survival and temperature of each group of animals respectively. Analyses were done with Tukey multiple comparison test using GraphPad Prism version 5 for Windows. Survival proportion analysis was done by Log-rank Mantel-Cox Test.

Fig. 1
Shows the haematological parameters of laboratory animals passaged with T. brucei. A. Detailed packed cell volume data, B. white blood cell data and C. red blood cell data from five passages.
Fig. 2
Percentage weight difference of laboratory animals at the peak of parasitaemia for different passages.
Fig. 3
Course of parasitaemia over five passages of the groups. Each point represents the mean±SEM.
Fig. 4
Log-rank Mantel-Cox Test of survival proportions for laboratory animals infected with Trypanosoma brucei.
Table 1
Mean survival time of infected laboratory animals.
Table 2
Average temperature (°C) at parasitaemia peak.

2. Experimental design, materials and methods

Datasets were generated with the permission and guidelines of the University of Ibadan animal ethics committee (UI-ACUREC/App/2015/019). Three groups of laboratory animals A, B and C with fifteen rabbits, fifteen rats and fifteen mice respectively, with each group divided into five treatments of three animals each in a treatment per passages (i.e. A1–A5 representing first to the fifth passages). Each treatment was kept in a transparent netted cage (30 × 45 cm) which allows proper ventilation. All the laboratory animals were fed ad-libitum with pelletized rodent feed and clean water, they were acclimatized for two weeks. Infective blood with Trypanosoma brucei was obtained from an infected donor Swiss albino mouse (from the Institute of Advanced Malaria Research, College of Medicine, University of Ibadan, Nigeria). After the proper dilution factor (1:10), 0.2 mL containing 105 of trypanosomes was inoculated intraperitoneally to infect the laboratory animals.

At peak parasitaemia of first passage, trypanosomes were assessed and inoculated into the next sets of animals for the second passage, until the fifth passage was achieved. Groups D and E contains fifteen mice each, these were infected with T. brucei from rabbits (Group A) and rats (Group B) respectively. Group A (rabbits) at the peak of parasitaemia were inoculated into group D (i.e. D1 – first passage from A1 to D5 – fifth passage from A5), while group E is produced from successive passages from group B (rat) in the same manner. Wet films of the blood from the T. brucei infected mice were made under a 7 × 22 mm cover glass. The films were examined under ×400 magnification, and a field was chosen in which the cells were evenly distributed. Giemsa-stained thin smears of blood were also prepared to observe diverse types of pleomorphic trypanosomes and white blood cells present. Parasitaemia was monitored daily from equivalent log number of organisms per millimeter of blood as described by Herbert and Lumsden [1].

Haematological analysis was based on method described [2], and Packed cell volume (PCV) was measured using micro haematocrit method. White blood cell count (WBC) and red blood cells (RBC) were determined in a coulter counter (Cyan Haemocytometer, Belgium), and temperature monitored using digital thermometer. The weight of all the animals have been included in the dataset supplementary files for assessment. Percentage weight difference dataset was necessary to compare the differences between separate groups (Fig. 2).

Acknowledgements

We appreciate the experimental animal unit at University of Ibadan for making the laboratory animals available at due time.

Footnotes

Transparency documentTransparency document associated with this article can be found in the online version at http://dx.doi.org/ 10.1016/j.dib.2017.08.022.

Appendix ASupplementary data associated with this article can be found in the online version at http://dx.doi.org/ 10.1016/j.dib.2017.08.022.

Transparency document. Supplementary material

Transparency document

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Appendix A. Supplementary material

Supplementary material

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References

1. Herbert W.J., Lumsden W.H. Trypanosoma brucei: a rapid "matching" method for estimating the host's parasitaemia. Exp. Parasitol. 1976;40:427–431. [PubMed]
2. Jain N. 4th ed. Lea and Febiger; Philadephia: 1986. Schalm’s Veterinary Hematology; pp. 610–612.

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