|Home | About | Journals | Submit | Contact Us | Français|
Scientist in developing countries maintain trypanosomes in laboratory animals for in vivo experiments. We generated data on the adaptation of Trypanosoma brucei (NITR201 strain) in balb/c mice (forty-five, 18–23 g), wistar rats (fifteen, 180–220 g) and New Zealand white and chinchilla rabbits (fifteen, 2.8–3.0 kg) in a controlled experimental system. The weight, haematological parameters, course of parasitaemia, temperature, mean survival time and survival proportions of laboratory animals in groups A–E were collected and analysed for differences in response to the same challenge of quantity, strain and species of T. brucei. Trypanosome pleomorphism of long, intermediate to short-stumpy forms were among the dataset counts for parasitaemia. Statistical data after analysis were summarised in the supplementary file to show the differences and corresponding reaction of multiple passages.
Value of the data
The dataset of this article provides an extensive information on physiologic and haematologic parameters associated with trypanosome continuous passaging in three laboratory animals. Analysed data on differences of the animal physiology to trypanosome is in Fig. 1, Fig. 2, Fig. 3, Fig. 4. Table 1, Table 2 show mean survival and temperature of each group of animals respectively. Analyses were done with Tukey multiple comparison test using GraphPad Prism version 5 for Windows. Survival proportion analysis was done by Log-rank Mantel-Cox Test.
Datasets were generated with the permission and guidelines of the University of Ibadan animal ethics committee (UI-ACUREC/App/2015/019). Three groups of laboratory animals A, B and C with fifteen rabbits, fifteen rats and fifteen mice respectively, with each group divided into five treatments of three animals each in a treatment per passages (i.e. A1–A5 representing first to the fifth passages). Each treatment was kept in a transparent netted cage (30 × 45 cm) which allows proper ventilation. All the laboratory animals were fed ad-libitum with pelletized rodent feed and clean water, they were acclimatized for two weeks. Infective blood with Trypanosoma brucei was obtained from an infected donor Swiss albino mouse (from the Institute of Advanced Malaria Research, College of Medicine, University of Ibadan, Nigeria). After the proper dilution factor (1:10), 0.2 mL containing 105 of trypanosomes was inoculated intraperitoneally to infect the laboratory animals.
At peak parasitaemia of first passage, trypanosomes were assessed and inoculated into the next sets of animals for the second passage, until the fifth passage was achieved. Groups D and E contains fifteen mice each, these were infected with T. brucei from rabbits (Group A) and rats (Group B) respectively. Group A (rabbits) at the peak of parasitaemia were inoculated into group D (i.e. D1 – first passage from A1 to D5 – fifth passage from A5), while group E is produced from successive passages from group B (rat) in the same manner. Wet films of the blood from the T. brucei infected mice were made under a 7 × 22 mm cover glass. The films were examined under ×400 magnification, and a field was chosen in which the cells were evenly distributed. Giemsa-stained thin smears of blood were also prepared to observe diverse types of pleomorphic trypanosomes and white blood cells present. Parasitaemia was monitored daily from equivalent log number of organisms per millimeter of blood as described by Herbert and Lumsden .
Haematological analysis was based on method described , and Packed cell volume (PCV) was measured using micro haematocrit method. White blood cell count (WBC) and red blood cells (RBC) were determined in a coulter counter (Cyan Haemocytometer, Belgium), and temperature monitored using digital thermometer. The weight of all the animals have been included in the dataset supplementary files for assessment. Percentage weight difference dataset was necessary to compare the differences between separate groups (Fig. 2).
We appreciate the experimental animal unit at University of Ibadan for making the laboratory animals available at due time.