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ISME J. 2017 September; 11(9): 1943–1948.
Published online 2017 April 14. doi:  10.1038/ismej.2017.56
PMCID: PMC5563948

Soil aggregates as massively concurrent evolutionary incubators

Introduction

Soil aggregation, a key component of soil structure, has mostly been examined from the perspective of soil management and the mediation of ecosystem processes such as soil carbon storage. However, soil aggregation is also a major factor to consider in terms of the fine-scale organization of the soil microbiome. For example, the physico-chemical conditions inside of aggregates usually differ from the conditions prevalent in the bulk soil and aggregates therefore increase the spatial heterogeneity of the soil. In addition, aggregates can provide a refuge for microbes against predation since their interior is not accessible to many predators. Soil aggregates are thus clearly important for microbial community ecology in soils (for example, Vos et al., 2013; Rillig et al., 2016) and for microbially driven biogeochemistry, and soil microbial ecologists are increasingly appreciating these aspects of soil aggregation. Soil aggregates have, however, so far been neglected when it comes to evolutionary considerations (Crawford et al., 2005) and we here propose that the process of soil aggregation should be considered as an important driver of evolution in the soil microbial community.

There are several features that make soil aggregates specifically interesting, and perhaps even unique, in terms of a setting for microbial evolution (Table 1). Soil aggregation is a continuous and dynamic process in which the formation and disintegration of individual micro- and macroaggregates are separated in time by periods of relative stability. Each individual soil aggregate may provide a unique environmental compartmentalization of the soil microbial community that is, to a large extent, isolated from its surroundings and that can be thought of as an ‘incubator’ for microbial evolutionary change. Because of their isolation, different aggregates can be regarded as ‘concurrent incubators’ that allow enclosed microbial communities to pursue their own independent evolutionary trajectories during their lifetime (‘incubation period’). The huge number of aggregates that exists at any moment in time validates their conceptualization as ‘massively concurrent incubators’ for microbial evolutionary change (Figure 1). Upon disintegration of soil aggregates (‘incubation cycle ends’), formerly enclosed microbial communities are released and allowed to interact with the microbial community of the soil at large.

Figure 1
Evolutionary change in soil aggregates: the life cycle of a soil aggregate microbial ‘incubator’ (meaning a compartmentalized microbial habitat). (a) Depicts the dynamics of the soil aggregate life cycle, with the stage formation, stabilization/incubation ...
Table 1
Qualitative comparison of various microbial habitat types in terms of features that are conducive to the formation of an evolutionary incubator for communities

This combination of features (isolation, large number and relative stability) sets soil aggregates apart from other microbial habitats that may also provide temporary isolation of microbial communities, such as the animal intestinal tract and other parts of the animal body (see also Cordero and Datta, 2016), leaves, roots, and many aquatic habitats (Table 1). However, these habitats do not provide the same combination of extent of isolation, duration of isolation and number of concurrent ‘incubators’ as soil aggregates do.

We discuss these specific characteristics of soil aggregates next, before describing how evolutionary change in aggregates can occur and explaining how this system can be tackled empirically.

Characteristics of soil aggregates important for microbial evolution

Number of aggregates

Soil aggregates are exceptionally numerous. They are relatively small (microaggregates are <250 μm, macroaggregates range from 250 μm to a few mm in diameter) and soil essentially consists of packed aggregates and pore spaces. Thus, per square meter of soil surface in any terrestrial ecosystem, there is an enormous number of soil aggregates of various sizes. This is important, because each one of these aggregates can be viewed as an independent evolutionary ‘incubator’, having encased its own microbial community during its formation.

Relative isolation

Soil aggregate interiors are quite isolated from the surrounding matrix. This is illustrated by very steep gradients in gas concentrations, for example, oxygen, from the inside to the outside of aggregates (Sexstone et al., 1985). This means that even the exchange of gases, let alone cells, is highly limited. Bacterial cells do possess mobility, and can move towards, and perhaps to a limited extent, also into aggregates (Dechesne et al., 2010), but the vast majority of the aggregate interior is blocked from dispersal. This degree of isolation is important for the trajectories of evolutionary changes that can occur inside of the aggregates (Vetsigian et al., 2011), which are largely unencumbered by migration.

Stability

Stability is important to allow time for evolutionary changes. By their very nature, aggregates are a structural component and thus a certain stability is inherent in their very existence. Aggregate stability is a property routinely measured in soil science. Soil science has also been concerned with describing and understanding the binding agents, and biotic players that mediate this stability. It is thus well known that these habitats can be stable at any given time, when examined at the level of entire aggregate populations. The dynamic nature of habitat turnover is less well understood, but the few measurements using tracers, most notably rare earth element oxides, have indicated that microaggregates are stable for months and macroaggregates for weeks to months (De Gryze et al., 2006). Most likely the range of aggregate stability times, depending on the site conditions, extends up to years.

Breakdown

The eventual breakdown of these evolutionary ‘incubators’ is also important, because this releases the entrapped microbial populations. Once aggregates are formed and stabilized by a variety of biota and chemical binding agents, they are subject to disintegration (Six et al., 2004). Disintegration can occur via a number of disintegrating forces, including water, tillage and others (for example, earthworms, pressure exerted by plant roots), and by soil organic matter decomposition. When an aggregate falls apart, the interior soil microbial community, with the evolutionary changes having occurred following the period of sequestration, is liberated and then this community is free to interact with the surrounding soil community (Rillig et al., 2016). This latter point is important, because now any evolutionary changes can become part of the ‘mainstream’ soil community, for example, via replacement of phylotypes or by horizontal gene transfer.

Evolutionary change in soil aggregates

We define microbial evolution in soil aggregates as any change in the relative allele frequencies or phylotype frequencies of the microbial populations enclosed within an aggregate; a change in allele frequency can arise by natural selection, migration, non-random mating (for example, outbreeding in heterothallic fungi) or genetic drift. In addition, de novo changes, for example, via horizontal gene transfer or by mutation, can occur within a lineage during the aggregate ‘incubation’ period. We divide our discussion of evolutionary forces into the three phases of the aggregate life cycle: aggregate formation, the stable aggregate period and the eventual disintegration (Figure 1a).

Aggregate formation: founder effects

During the formation of a soil aggregate, populations of microbial phylotypes present in the bulk soil are enclosed within the aggregate structure (Figure 1b). Although actual initial sizes of the enclosed microbial communities are unknown, a size range of several thousands to several millions of individuals is plausible depending on aggregate size and assuming a passive enclosure of microorganisms present in the bulk soil from which the aggregate was formed (Whitman et al., 1998). In any case, the enclosed population of any one phylotype is expected to be founded by a small number of individuals compared to the size of the source population in the bulk soil and founder effects are therefore likely. Such effects occur when only a fraction of the genetic diversity of the source population is represented within the newly founded population (genetic bottleneck) and the initial relative allele frequencies within the founded population are different from the frequencies in the source population. This may lead to low levels of genetic diversity within populations enclosed in new aggregates and high levels of genetic differentiation between populations enclosed in neighboring aggregates.

Inside the aggregate interior: genetic drift, natural selection and limited gene flow

Once formed, the aggregate constitutes an environment that is significantly different and isolated from the surrounding soil. Differences include altered availability of terminal electron acceptors, depletion of sources of organic carbon and nutrients, and potential accumulation of antibiotics or toxins (Vos et al., 2013). In addition, aggregate interiors are spatially heterogeneous environments as a consequence of the hierarchical nature of their assembly: macroaggregates contain microaggregates, which either formed inside the macroaggregate or existed before and were integrated when the aggregate was formed, and pieces of particulate organic matter as well as interior pore spaces (Figure 1a). Selection pressures due to the different environmental conditions in the aggregate interiors and strong species interactions, especially competition (Barraclough, 2015), may lead to a decrease of genetic diversity in enclosed populations. In addition to natural selection, genetic drift will likely be a significant evolutionary force that further reduces genetic diversity (Figure 1b). Isolation of the enclosed microbial community prevents gene flow from countering the negative effects of natural selection and genetic drift on its genetic diversity. However, spatial heterogeneity within the aggregate and increased mutation rates due to elevated stress levels (for example, due to nutrient depletion and toxin accumulation) may, to a certain extent, maintain or increase genetic diversity within the aggregate. For example, in Escherichiacoli genomes mutation rates can drastically increase and this generates variants including those with diverse metabolic capabilities (for example, Saint-Ruf et al., 2014). In addition, selection pressures may be highly temporally and spatially dynamic among aggregate interiors within a population of aggregates due to differences in the aggregation process and the particular founding subsample of the microbiome included in the aggregate.

Aggregate disintegration: natural selection and hybrid genotypes

Upon disintegration of soil aggregates (Figure 1b), the enclosed microbial communities are released and subjected to environmental conditions that differ from the aggregate interior. In addition, they will interact with the microbial community of the bulk soil including released communities of neighboring aggregates that disintegrated. Merging of these differentiated communities (genetic admixture) may increase the overall genetic diversity of the soil microbiome and, especially in the case of sexually reproducing species, has the potential to generate hybrid genotypes with higher levels of fitness (heterosis or hybrid vigor) that may spread through the soil.

Overall evolutionary consequences for soil biota diversity: aggregates as the cradle of soil biodiversity?

It should be clear from the discussion above that the continuous formation and disintegration of vast amounts of soil aggregates catalyzes evolutionary processes in the soil microbial community and may shape its evolutionary pathway. Evolutionary processes within each single aggregate are thereby likely to a decrease the genetic diversity of the enclosed microbial community. However, we hypothesize that the effect of having soil aggregates—compared to a well-mixed habitat or even a statically spatially heterogeneous habitat—will lead to the generation of higher levels of genetic diversity in the soil biota as a whole. The main reason is the concurrent nature of a great diversity of starting conditions, founder effects and subsequent independent evolutionary trajectories pursued by the microbial populations inside of aggregates.

How to study microbial evolution in soil aggregates

Empirical as well as modeling approaches can be used to study the evolutionary aspects of soil aggregation. Computational modeling allows to study systems for which experimental solutions are not readily available in microbial ecology (Widder et al., 2016). However, basic features will likely have to be first explored by generating empirical data.

Whole-genome sequencing has opened paths to the study of evolution of pathogenic bacteria within the human body, as recently reviewed by Didelot et al. (2016) and Biek et al. (2015). These methods have recently become feasible because of the greatly reduced sample analysis costs. Such approaches and the toolbox developed for studies in a more clinical/epidemiological context (that is, isolation, genome sequencing, assembly and comparative genome analysis) could also be brought to bear on the situation in soil aggregates. Specifically, strains of a particular target species could be isolated from various co-occurring soil aggregates (similarly to Vetsigian et al. (2011) or Scheinin et al. (2015)) and then subjected to whole-genome sequencing. In addition, the recently developed maximum-depth sequencing approach and error-corrected high-throughput sequencing method could be employed (Jee et al., 2016). Often isolation of microbes will not be possible; in such cases, single-cell genomics or metagenomics techniques would be the method of choice, coupled with the ever-increasing arsenal of omics techniques for functional characterization (for example, proteomics, metabolomics). Ideally, such sequencing should be accompanied by assessments of microbial community composition in aggregates, and a monitoring of environmental microsite conditions to facilitate a better understanding of the mechanisms underlying such changes.

In addition to purely observational sequencing-based approaches, also direct experimental approaches could be pursued. Such experiments should start with using model bacterial systems with well-characterized players (for example, in terms of reference genomes) that occur in soils, such as Bacillus subtilis or Pseudomonas aeruginosa. Aggregates could be artificially formed in the lab, encapsulating starting cultures of such strains with known characteristics, and then evolution with and without aggregate association could be directly compared. Given the absence of other microbes, strains could be easily re-isolated for whole-genome sequencing. Such systems could then used as a starting point to increase complexity in a stepwise fashion, for example by incrementally including other members of the microbial community, including other bacteria and also fungi, and also representatives of other trophic levels. Eventually, questions of a more complex or applied nature, or in the context of global change could be tackled (Table 2).

Table 2
Examples of questions arising from the study of soil aggregates as unique environmental compartmentalizations for microbial evolution

Conclusions

There is a great general need to build a predictive understanding of the ecological and evolutionary dynamics of microbial communities (Widder et al., 2016). For soils, this is especially true, given the importance of soil microbes in global biogeochemical cycles, human and ecosystem health (Wall et al., 2015), for agricultural sustainability and the risks in store in the future in terms of extinctions of soil microbial taxa (Veresoglou et al., 2015). Clearly, in microbial systems, it is important to consider evolutionary processes when studying communities due to the frequent coincidence of ecological and evolutionary timescales. Explicitly focusing on soil aggregates holds great promise of uncovering a possible major element mediating evolutionary change within soil microbial communities.

Acknowledgments

MCR acknowledges funding from Deutsche Forschungsgemeinschaft (DFG) and from the ERC Advanced Grant Gradual Change. We thank the reviewers for their helpful comments.

Footnotes

The authors declare no conflict of interest.

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