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Logo of neuroscibullNeuroscience Bulletin
Neurosci Bull. 2008 June; 24(3): 150.
Published online 2008 July 19. doi:  10.1007/s12264-008-1702-1
PMCID: PMC5552545

Language: English | Chinese

Effect of resuscitation after selective cerebral ultraprofound hypothermia on expressions of nerve growth factor and glial cell line-derived neurotrophic factor in the brain of monkey




To investigate the expression of nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) in monkeys of resuscitation after selective cerebral ultraprofound hypothermia and blood flow occlusion.


The monkeys were immediately removed brain after death in operation of group A (identical temperature perfusion group) and group B (ultraprofound hypothermia perfusion group). Immunohistochemical technique was used to determine frontal cellular expression of NGF and GDNF. Statistics were analyzed by ANOVA analyses with significance level at P < 0.05.


The expressions of NGF and GDNF in the group B were significantly higher than those in the group A (P < 0.05).


NGF and GDNF increased significantly in the monkeys of resuscitation after selective cerebral ultraprofound hypothermia and blood flow occlusion. It may be a protective mechanism for neuron survival and neural function recovery.

Keywords: nerve growth factor, glial cell line-derived neurotrophicfactor, ultraprofound hypothermic circulatory arrest, resuscitation, monkey brain



观察常温缺血10 min后选择性超深低温断血流复苏后猴脑中神经生长因子(nerve growth factor, NGF)和胶质细胞源性神经营养因子(glial cell line-derived neurotrophicfactor, GDNF)表达的变化。


等温组及超深低温组实验猴于灌注或复苏死亡后立即开颅取脑, 用NGF和GDNF抗体进行免疫组化染色;对额叶恒定视野内NGF和GDNF 的阳性细胞记数求阳性率, 并统计学分析。


等温组2 只实验猴额叶NGF 和GDNF 有微量表达, 超深低温组4 只实验猴额叶NGF 和GDNF 表达明显上调, 与等温组比较差异均极显著(P < 0.01)。


猴脑选择性超深低温断血流复苏实验可引起NGF和GDNF表达上调, 这可能是防止脑缺血的重要保护机制之一。

关键词: 神经生长因子, 胶质细胞源性神经营养因子, 超深低温断血流, 复苏, 猴脑


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