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To investigate the expression of nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) in monkeys of resuscitation after selective cerebral ultraprofound hypothermia and blood flow occlusion.
The monkeys were immediately removed brain after death in operation of group A (identical temperature perfusion group) and group B (ultraprofound hypothermia perfusion group). Immunohistochemical technique was used to determine frontal cellular expression of NGF and GDNF. Statistics were analyzed by ANOVA analyses with significance level at P < 0.05.
The expressions of NGF and GDNF in the group B were significantly higher than those in the group A (P < 0.05).
NGF and GDNF increased significantly in the monkeys of resuscitation after selective cerebral ultraprofound hypothermia and blood flow occlusion. It may be a protective mechanism for neuron survival and neural function recovery.
观察常温缺血10 min后选择性超深低温断血流复苏后猴脑中神经生长因子(nerve growth factor, NGF)和胶质细胞源性神经营养因子(glial cell line-derived neurotrophicfactor, GDNF)表达的变化。
等温组及超深低温组实验猴于灌注或复苏死亡后立即开颅取脑, 用NGF和GDNF抗体进行免疫组化染色;对额叶恒定视野内NGF和GDNF 的阳性细胞记数求阳性率, 并统计学分析。
等温组2 只实验猴额叶NGF 和GDNF 有微量表达, 超深低温组4 只实验猴额叶NGF 和GDNF 表达明显上调, 与等温组比较差异均极显著(P < 0.01)。