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To investigate the expressions of Galectin-3 (Gal-3), Bcl-2 and Bax in human pituitary adenomas, and to explore the interrelation among them.
RT-PCR and immunohistochemistry were applied to detect the mRNA and protein expressions of Gal-3, Bcl-2 and Bax in surgically excised human pituitary adenoma tissues, including invasive and non-invasive pituitary adenomas, and the correlation analysis was performed.
The Gal-3 and Bcl-2 expressions in the invasive pituitary group were significantly higher than those in the non-invasive group, and the expression of Bax had no significant difference between the two groups. Pearson’s correlation analyses showed that the Gal-3 expression was positively correlated with Bcl-2, but was not correlated with Bax, which was inversely correlated with expression of Bcl-2.
Gal-3 may function through a cell death inhibition pathway involving Bcl-2 to enhance cell proliferation, which result in the invasive growth of pituitary adenoma. These results indicate that Gal-3 has an important role in pituitary tumor cell proliferation and may serves as a possible therapeutic target in treatment of pituitary tumors.
探讨垂体腺瘤中半乳糖凝集素-3(Galectin-3, Gal-3)和凋亡相关基因 Bcl-2、 Bax 的内在联系。
RT-PCR检测20例垂体腺瘤Gal-3、 Bcl-2和Bax mRNA的表达; 免疫组化染色检测78例垂体腺瘤中Gal-3、 Bcl-2和 Bax 蛋白表达, 分析侵袭性和非侵袭性垂体腺瘤中Gal-3、 Bcl-2 和Bax 表达的差异及其相关关系。
侵袭组垂体腺瘤Gal-3和Bcl-2 mRNA和蛋白的表达水平较非侵袭组显著增高, 而Bax mRNA 和蛋白在两组中的表达水平无显著差异。 等级相关分析显示Gal-3 蛋白与Bcl-2 蛋白之间有正直线相关关系(r = 0.291, P = 0.01); Gal-3 蛋白与Bax蛋白之间无直线相关关系(r = −0.023, P > 0.05); Bcl-2 蛋白与Bax蛋白表达之间的Pearson相关系数为r = −0.267, P < 0.05(双侧), 呈负相关关系。
Gal-3 可能主要是通过与Bcl-2 协同作用, 发挥其抗凋亡效应, 相对促进, 体腺瘤细胞增殖, 导致垂体腺瘤的侵袭性生长。这些结果提示Gal-3 在垂体肿瘤细胞增殖过程中发挥重要作用, 有望作为垂体腺瘤的一个治疗靶点。