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Logo of neuroscibullNeuroscience Bulletin
 
Neurosci Bull. 2007 May; 23(3): 180–184.
Published online 2008 February 3. doi:  10.1007/s12264-007-0027-9
PMCID: PMC5550634

Language: English | Chinese

Effect of nitric oxide on the expression of apelin receptor mRNA in rat caudate nucleus

大鼠尾核内一氧化氮对apelin 受体 mRNA 表达的影响

Abstract

Objective

To investigate the effect of nitric oxide (NO) on the expression of apelin receptor mRNA, as well as their correlation, in the caudate nucleus of rat.

Methods

L-Arginine (L-Arg), N G-nitro-L-arginine methyl ester (L-NAME) and normal saline (NS) was separately microinjected into rat caudate nucleus. Expressions of neuronal NO synthase (nNOS) mRNA and apelin receptor mRNA were detected by RT-PCR at 4, 8, 12, 24 and 48 h after microinjection, and their correlation was determined.

Results

The expressions of nNOS mRNA and apelin receptor mRNA were both significantly increased after microinjection of L-Arg, but significantly decreased after microinjection of L-NAME compared with the NS control group. The nNOS mRNA had a positive correlation with the expression of apelin receptor mRNA after microinjection of L-Arg and L-NAME.

Conclusion

The activity of NOS in the central nervous system, especially in the caudate nucleus, is one of the key factors for NO to exert many kinds of biological actions, such as modulation of central pain, as a neurotransmitter. The neurobiological action of NO in rat caudate nucleus may be associated with apelin receptors.

Keywords: nitric oxide, apelin receptor, pain, caudate nucleus, reverse transcription polymerase chain reaction

摘要

目的

研究大鼠尾核内一氧化氮(nitric oxide, NO)的作用机理及其与apelin 受体mRNA 表达的相关性。

方法

大鼠尾核内微量注射NO前体L-精氨酸(L-Arg)、 N G-硝基-L-精氨酸甲酯(N G-nitro-L-arginine methyl ester, L-NAME)和生理盐水(NS), 应用逆转录-聚合酶链反应(RT-PCR)检测大鼠尾核给药4、 8、 12、 24 及 48 h 后一 氧化氮合酶(neuronal nitric oxide synthase, nNOS) mRNA 和 apelin 受体 mRNA 表达的变化及二者的相关性。

结果

注射 L-Arg 后大鼠尾核nNOS和apelin受体的mRNA表达明显升高, 注射 L-NAME 后大鼠尾核nNOS和apelin 受体mRNA表达明显降低, 且均有统计学意义。 注射L-Arg 和L-NAME 后, nNOSmRNA 和 apelin 受体 mRNA 的表达呈正相关。

结论

在中枢神经系统, 尤其是在尾核中, NOS的活性是 NO 作为神经递质或调质参与包括 中枢痛觉调制作用在内的多种生物学作用的关键因素之一, 尾核内NO的神经生物学作用可能与apelin受体作用相关。

关键词: 一氧化氮, apelin 受体, 痛觉, 尾核, 逆转录-聚合酶链反应

References

[1] Ciani E., Guidi S., Bartesaghi R., Contestabile A. Nitric oxide regulates cGMP-dependent cAMP-responsive element binding protein phosphorylation and Bcl-2 expression in cerebellar neurons: implication for a survival role of nitric oxide. J Neurochem. 2002;82:1282–1289. doi: 10.1046/j.1471-4159.2002.01080.x. [PubMed] [Cross Ref]
[2] Haley J.E. Gases as neurotransmitters. Essays Biochem. 1998;33:79–91. [PubMed]
[3] Masri B., Knibiehler B., Audigier Y. Apelin signalling: a promising pathway from cloning to pharmacology. Cell Signal. 2005;17:415–426. doi: 10.1016/j.cellsig.2004.09.018. [PubMed] [Cross Ref]
[4] Jaszberenyi M., Bujdoso E., Telegdy G. Behavioral, neuroendocrine and thermoregulatory actions of apelin-13. Neuroscience. 2004;129:811–816. doi: 10.1016/j.neuroscience.2004.08.007. [PubMed] [Cross Ref]
[5] Liu Y.H., Liu W.Y., Liu H.Q., Bai B. The involvement of nitric oxide in pain modulation in rat caudate nucleus. Chin J Pain Med. 2006;12:163–166.
[6] Pellegrino L. J. A stereotaxic atlas of rat brain. 2. New York: Plenum Press; 1979. pp. 18–21.
[7] Schmittgen T.D., Zakrajsek B.A. Effect of experimental treatment on housekeeping gene expression: validation by realtime, quantitative RT-PCR. J Biochem Biophys Methods. 2000;46:69–81. doi: 10.1016/S0165-022X(00)00129-9. [PubMed] [Cross Ref]
[8] Meller S.T., Pechman P.S., Gebhart G.F., Maves T.J. Nitric oxide mediates the thermal hyperalgesia produced in a model of neuropathic pain in the rat. Neuroscience. 1992;50:7–10. doi: 10.1016/0306-4522(92)90377-E. [PubMed] [Cross Ref]
[9] Lowenstein C.J., Glatt C.S., Bredt D.S., Snyder S.H. Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme. Proc Natl Acad Sci USA. 1992;89:6711–6715. doi: 10.1073/pnas.89.15.6711. [PubMed] [Cross Ref]
[10] Xu J.Y., Hill K.P., Bidlack J.M. The nitric oxide/cyclic cGMP system at the supraspinal site is involved in the development of acute morphine antinociceptive tolerance. Pharmacol Exp Ther. 1998;284:196–201. [PubMed]
[11] Chan M.H., Chien T.H., Lee P.Y., Chen H.H. Involvement of NO/cGMP pathway in toluene-induced locomotor hyperactivity in female rats. Psychopharmacology (Berl) 2004;176:435–439. doi: 10.1007/s00213-004-1900-0. [PubMed] [Cross Ref]
[12] Losano G., Penna C., Cappello S., Pagliaro P. Activity of apelin and APJ receptors on myocardial contractility and vasomotor tone. Ital Heart J Suppl. 2005;6:272–278. [PubMed]
[13] Tatemoto K., Takayama K., Zou M.X., Kumaki I., Zhang W., Kumano K., et al. The novel peptide apelin lowers blood pressure via a nitric oxide-dependent mechanism. Regul Pept. 2001;99:87–92. doi: 10.1016/S0167-0115(01)00236-1. [PubMed] [Cross Ref]
[14] Szokodi I., Tavi P., Foldes G., Voutilainen-Myllyla S., Ilves M., Tokola H., et al. Apelin, the novel endogenous ligand of the orphan receptor APJ, regulates cardiac contractility. Circ Res. 2002;91:434–440. doi: 10.1161/01.RES.0000033522.37861.69. [PubMed] [Cross Ref]
[15] Charles C.J., Rademaker M.T., Richards A.M. Apelin-13 induces a biphasic haemodynamic response and hormonal activation in normal conscious sheep. J Endocrinol. 2006;189:701–710. doi: 10.1677/joe.1.06804. [PubMed] [Cross Ref]

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