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Logo of neuroscibullNeuroscience Bulletin
 
Neurosci Bull. 2007 May; 23(3): 175–179.
Published online 2008 February 3. doi:  10.1007/s12264-007-0026-x
PMCID: PMC5550633

Language: English | Chinese

Localization of vesicular glutamate transporters in the peripheral vestibular system of rat

囊泡膜谷氨酸转运体在前庭外周系统中的分布

Abstract

Objective

To examine the vesicular glutamate transporters (VGluTs: VGluT1-VGluT3) in the peripheral vestibular system.

Methods

The vestibular structures, including Scarpa’s ganglion (vestibular ganglion, VG), maculae of utricle and saccule, and ampullary cristae, from normal Sprague-Dawley rats were processed immunohistochemically for VGluTs, by avidin-biotinylated peroxidase complex method, with 3-3′-diaminobenzidine (DAB) as chromogen.

Results

(1) VGluT1 was localized to partial neurons of VG and to the putative primary afferent fibers innervating vestibular end-organs. (2) Intense VGluT3 immunoreactivity was detected in large number of sensory epithelia cells, and weak labeling of VGluT3-positive afferent fibers was in the maculae and ampullary cristae. (3) No or very weak VGluT2 immunoreactivity was observed in the VG and acoustic maculae.

Conclusion

These results provide the morphological support that glutamate exists in the peripheral vestibular system, and it may play an important role in the centripetal vestibular transmission.

Keywords: vesicular glutamate transporter, acoustic maculae, Scarpa’s ganglion, immunohistochemistry, rat

摘要

目的

检查囊泡膜谷氨酸转运体(vesicular glutamate transporter, VGluT)在前庭外周系统中的分布特征。

方法

采用ABC (avidin-biotinylated peroxidase complex)免疫组织化学方法, 二氨基联苯作为染色剂, 观察 VGluT1-3在正常成年SD大鼠前庭外周系统, 包括球囊、 椭圆囊、 壶腹嵴和前庭神经节 (Scarpa神经节)的表达。

结果

(1) VGluT1样免疫阳性产物位于前庭神经节和传入纤维支配的前庭外周终末感受器。 (2)大部分感觉上皮细 胞表达高密度的VGluT3 样免疫阳性产物, 但在球囊斑和椭圆囊斑, VGluT3 样免疫阳性传入纤维表达较弱。 (3) 在上述部位, 没有或仅有很弱的VGluT2样免疫反应。

结论

VGluT1和VGluT3参与了初级前庭传入纤维和毛细胞中将谷氨酸转运入囊泡的过程, 可能在调节前庭终末感受器通过前庭核向大脑传递信息的传导通路中具有重要作用。

关键词: 囊泡膜谷氨酸转运体, 位觉斑, 前庭神经节, 免疫组织化学, 大鼠

References

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Articles from Neuroscience Bulletin are provided here courtesy of Springer