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To investigate the cell proliferation and differentiation in the developing brain of mouse.
C57/BL6 mice were divided into 3 groups at random. Bromodeoxyuridine (BrdU) was injected into the brains in different development periods once a day for 7 d. The brains were retrieved 4 weeks after the last BrdU injection. Immunohistochemical and immunofluorescent studies were carried out for detecting cell proliferation (BrdU) and cell differentiation (NeuN, APC, Iba1, and S100β), respectively.
The number of BrdU labeled cells decreased significantly with the development of the brain. Cell proliferation was prominent in the cortex and striatum. A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus.
These results demonstrated that cell proliferation, differentiation and survival were age and brain region related.
C57/BL6 小鼠分别于出生后10 天 (P10)、 17 天 (P17) 、 24 天 (P24) 不同脑发育期, 每天注射新生细胞标记物5-溴脱氧尿嘧啶核苷(BrdU), 连续注射7 天, 并分别于末次注射后四周将小鼠处死、 取脑。 采用免疫组化染色及免疫荧光染色分别检测细胞增生 (BrdU) 与细胞分化 (NeuN、 APC、 Iba1 和S100 β)。
细胞增生随着脑组织发育快速下降, 并以皮层和纹状体区细胞增生最显著。 皮层在发育早期以及纹状体和海马 CA区在发育各期检测到极少数为新生神经元细胞, 多数分化为胶质细胞; 海马齿状回以神经元细胞再生为主; 胶质细胞的再生随脑组织发育的成熟而逐渐减少。
研究证实小鼠脑组织细胞的增生、 分化以及存活与发育时期、 脑组织区域相关。