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In-vitro metabolite and drug detection rely on designed materials-based analytical platforms, which are universally used in biomedical research and clinical practice. However, metabolic analysis in bio-samples needs tedious sample preparation, due to the sample complexity and low molecular abundance. A further challenge is to construct diagnostic tools. Herein, we developed a platform using silver nanoshells. We synthesized SiO2@Ag with tunable shell structures by multi-cycled silver mirror reactions. Optimized nanoshells achieved direct laser desorption/ionization mass spectrometry in 0.5μL of bio-fluids. We applied these nanoshells for disease diagnosis and therapeutic evaluation. We identified patients with postoperative brain infection through daily monitoring and glucose quantitation in cerebrospinal fluid. We measured drug distribution in blood and cerebrospinal fluid systems and validated the function of blood-brain/cerebrospinal fluid-barriers for pharmacokinetics. Our work sheds light on the design of materials for advanced metabolic analysis and precision diagnostics.
In-vitro metabolic diagnosis relies on designed materials-based analytical platforms for detection of selected metabolites in biological samples, which has a key role for disease detection and therapeutic evaluation in clinics1, 2. Currently, both inorganic and organic materials have been used for metabolic analysis by selected spectroscopy methods, including nuclear magnetic resonance spectroscopy, biochemical analyzers, and mass spectrometry (MS) et al.1–5. In contrast with other methods, MS enjoys unique advantages of high accuracy, sensitivity, resolution, and throughput. However, the efficacy of MS and other spectroscopy methods is determined by rigorous sample pre-treatment procedures for enrichment or purification2, 6–9, due to the high sample complexity and low molecular abundance in biological samples. To date, MS-based analysis of metabolites in bio-fluids is still unsatisfactory in terms of selectivity, speed, costs, and reproducibility for clinical use. Therefore, to address the challenges in the sample pre-treatment, a designer material platform is in urgent demand for advanced in-vitro metabolic diagnosis.
Matrix-assisted laser desorption/ionization (MALDI) MS has displayed great superiority considering its fast analysis of analytes in seconds, accurate mass measurement for identification, simple sample preparation for broad application, and high sensitivity with low costs for practical use1, 2, 5. Inorganic particles (e.g., silicon10, 11, carbon12, 13, metal14–16, and metal oxide17, 18) have been preferred matrices in LDI MS for small metabolites as reported by research groups globally including ours. These particles can control the analytical variations and background signals in the mass region below m/z of 500, achieving the qualitative analysis of small metabolites, such as carbohydrates and amino acids6, 7, 19. Further coupling LDI MS with isotopic quantification, designed particles enabled metabolic analysis of rare cells, e.g., circulating tumor cells17. Notably, the LDI performance of particles is far from ideal for selective detection of small metabolites, dealing with complex bio-fluids in real case. As a result, the application of particle-assisted LDI MS for clinical diagnostics is very challenging and needs rational design of materials for use.
Plasmonic particles consist of noble metals including but not limited to Au, Ag, Pt, and Pd, which provide unique surface plasmon resonance and hot carriers20, 21 under laser irradiation ideal for matrix use. Despite that there have been many reports on the application of plasmonic particles for LDI MS6, 7, 14, 15, 19, most of current work are focused on the use of solid plasmonic particles in diverse shapes and there are very few reports on plasmonic nanoshells. Plasmonic Au nanoshells with nanoscaled surface roughness and higher hot carriers production over solid Au particles16, 22 have been demonstrated to be better candidates for LDI MS detection of small metabolites, whereas their application is very limited in complex bio-fluids for diagnostic purpose23. Notably, the analytical performance of plasmonic nanoshells depends on the selection of noble metals to adsorb ultraviolet (UV) laser and optimized shell structure for LDI MS detection. Designer plasmonic nanoshells would achieve efficient LDI MS for metabolic analysis in real case for clinical applications but have not been developed so far.
In this work, we report a platform based on designer plasmonic silver nanoshells for direct detection of small metabolites in clinical in-vitro metabolic diagnostics. We first synthesized a series of SiO2@Ag core-shell particles with tunable nanoshell structures through multi-cycled silver mirror reactions on the surface of SiO2 nanoparticles. The optimized plasmonic silver nanoshells as new matrices allowed fast, multiplex, sensitive, and selective LDI MS detection of small metabolites in 0.5μL of bio-fluids without enrichment or purification. Furthermore, coupling with isotopic quantification of selected metabolites, we demonstrated the use of these silver nanoshells for disease detection and therapeutic evaluation in clinics. For disease detection, we identified patients with postoperative brain infection through glucose quantitation and daily monitoring by cerebrospinal fluid (CSF) analysis. For therapeutic evaluation, we investigated drug distribution in blood and CSF systems and validated the function and permeability of blood–brain/CSF-barriers, during therapeutic treatment of patients with cerebral edema for pharmacokinetics study. Our work sheds light on the design of materials for high-performance metabolic analysis and precision diagnostics in real cases.
We synthesized silver nanoshells (also denoted SiO2@Ag) through a hard templating method (Fig. 1a), and the shell structures can be controlled using multi-cycled silver mirror reactions for LDI MS (Fig. 1b). The reaction temperature and time were critical and optimized in the synthesis process (Supplementary Fig. 1, see also “Methods” for details). We prepared mono-dispersed silica particles by the Stöber method with an average size of 181.8±12.53nm and polydispersity index (PDI) of 0.203±0.112 as the core materials (Supplementary Table 1; Supplementary Fig. 2). After the first cycle of silver mirror reaction, we anchored the silver nanoparticles with a size of ~2–4nm onto the surface of silica particles (SiO2@Ag-1, Fig. 1c) and high-resolution transmission electron microscopy (HRTEM) demonstrated the typical inter planar spacing of 2.04Å for silver composites along [2, 0, 0] direction (inset of Fig. 1c)24, 25. After multiple cycles (two cycles for SiO2@Ag-2, three cycles for SiO2@Ag-3, and four cycles for SiO2@Ag-4) of silver mirror reactions, we observed the gradual increase of shell thickness and silver nanoparticle size for SiO2@Ag-2 (Fig. 1d), SiO2@Ag-3 (Fig. 1e), and SiO2@Ag-4 (Fig. 1f), due to the seed-mediated growth process26, 27. We characterized the morphologies of SiO2@Ag, showing controlled nanoscaled surface roughness and nano-crevices under scanning electron microscopy (SEM) by the multi-cycled silver mirror reactions (Fig. 1c–f). As the electricity/heat insulating materials, the silica cores of SiO2@Ag contributed to produce more hot carriers20–22, 28 and retained the heat during LDI process, whereas the silver shell can be excited under 355nm of laser22, 23, 28. Moreover, as shown in the elemental mapping analysis (Supplementary Fig. 3)29, 30, the distribution of carbon (from the small metabolites), silver (from the shell, affording similar silver nanoparticles densities on surface to Fig. 1e with silver loading ratio of 22.80% by weight), and silicon (from the core) in the nanoshells–metabolites hybrids demonstrated the small molecules were trapped by nano-crevices on the surface of silver nanoshells, which would be crucial for efficient LDI of small metabolites.
We also examined the important structural parameters for LDI MS matrix use including the dispersity, zeta potential, surface area, composition, and light-absorbance of all prepared SiO2@Ag. In the dynamic light scattering (DLS) experiments, all SiO2@Ag showed fine dispersity in water with PDI of 0.105±0.064–0.313±0.044 (Supplementary Table 1), and the particle sizes increased from 185.8±0.611nm to 289.7±74.19nm (Fig. 1g) with multi-cycled silver mirror reactions, consistent with transmission electron microscopy (TEM) and SEM results confirming the controlled synthesis of SiO2@Ag. We obtained similar results for three batches of materials in parallel and demonstrated the reproducibility of the synthetic method with no significant difference (p<0.05, Supplementary Fig. 4a). SiO2@Ag particles were negatively charged with zeta potentials between −37.4±1.760mV and −22.2±1.130mV (Supplementary Table 1; Supplementary Fig. 5), beneficial for the formation of ion layers on the surface towards Na+/K+/Ag+ adduction. In the nitrogen adsorption analysis, we obtained type IV isotherms (Supplementary Fig. 6)31–34, suggesting a stacking mesoporous structure on the material surface. We calculated surface area of SiO2@Ag based on the multipoint Brunauer, Emmett, and Teller (BET) model31, 35 and SiO2@Ag enjoyed larger surface area (20.72±0.115–24.20±0.069m2g−1) compared with the bare SiO2 particles (18.58±0.095m2g−1) because of the increased surface roughness and introduction of silver (p<0.05, Supplementary Table 1). The increasing average size of silver nanoshells did not correlate to a similar increase in surface area due to the change of density similar to previous reports36, 37. To investigate the composition of materials, we recorded corresponding energy dispersive X-Ray (EDX) spectra (Fig. 1h; Supplementary Fig. 7), yielding an increasing silver loading ratio (by weight) of 0% for SiO2, 13.6% for SiO2@Ag-1, 19.75% for SiO2@Ag-2, 23.73% for SiO2@Ag-3 (consistent with Supplementary Fig. 3e), and 33.31% for SiO2@Ag-4.
To explore the light absorbance of particles, we performed ultraviolet-visible (UV-Vis) spectroscopy analysis in Fig. 1i and Supplementary Fig. 4b. The pure silica particles did not have any UV-Vis absorption peaks similar to reference reports23, 38, whereas the SiO2@Ag-1/2/3 particles displayed the absorption peak at ~436±2.45nm due to the Mie plasmon resonance excitation from the silver nanoparticles38, 39. After the fourth reduction cycle, the absorption peak red shifted to ~443±1.63nm for SiO2@Ag-4 (p<0.05), due to the enhanced plasmon coupling and aggregation of excess silver on the surface of silica core particles (agreed to TEM and SEM, Fig. 1f). The specific increase in the maximum absorption peak can be observed in Supplementary Fig. 4b for three batches of silver nanoshells in parallel. Notably, the absorption band of silver is close to 355nm (the wavelength for Nd:YAG laser used for LDI MS), beneficial for matrix use compared with gold nanoparticles16, 23, 40. The light absorbance increased and the plasmon resonance peak became broader with higher loading ratio of silver, agreed to TEM, SEM, DLS, and EDX characterizations demonstrating the adjustable properties of silver nanoshells through multi-cycled silver mirror reactions. Compared to previous literatures mostly focused on core-shell particles either with dispersed Ag nanoparticles (~4nm) on surface41 or thick (~10–80nm) densely packed shell structures39, 42 through surface coating techniques39, 41–44, our work was featured with SiO2@Ag affording thin (~2–10nm), discontinuous, and tunable silver nanoshells (Fig. 1c–e).
The surface chemistry of silver nanoshells can be adjusted considering the binding affinity between thiol groups and silver45, 46. We employed thiol-aptamers to modify the silver nanoshells and observed the new UV-Vis absorption peak at ~254nm for nucleic acid composites (Supplementary Fig. 8a), indicating the successful functionalization of aptamers14, 23, 46. We validated the surface chemistry of modified materials by Fourier transform infrared (FTIR) spectroscopy (Supplementary Fig. 8b). The appearance of typical peaks for –CH2– (at 2925cm−1 and 2851cm−1), –PO2 (at 1147cm−1 and 1249cm−1), and –C=O (at 1738 cm−1) confirmed the functional surface of silver nanoshells after modification with aptamers23, 34, which may allow target enrichment and selective detection of specific molecules.
To select the optimized materials, we applied the SiO2@Ag as new matrices in analysis of small metabolites, through mixing SiO2@Ag particles with different analytes solutions and drying the mixture for direct LDI MS. Notably, we obtained strong silver ions-adducted molecular peaks for all the spectra in Fig. 2, distinct from matrices composed of other noble metals6, 7, 14, 15, 19. We observed m/z of 289.26 [M+107Ag]+ and 291.26 [M+109Ag]+ for mannitol (Fig. 2a), m/z of 287.23 [M+107Ag]+ and 289.23 [M+109Ag]+ for glucose (Fig. 2b), and m/z of 256.20 [M+107Ag]+ and 258.20 [M+109Ag]+ for methionine (Fig. 2c). The peak intensities of [M+107Ag]+ and [M+109Ag]+ were close, evidencing the even isotope abundance of silver in nature. In the production of [M+Ag]+ ions, competitive Ag+ ions can cationize molecules containing π-bonds by the Dewar model47, 48 and polar functional groups (like hydroxyl group) through the ion–dipole interaction49, 50.
Specifically, SiO2@Ag-3 afforded the highest mean signal intensities in triplicate experiments, superior to SiO2 (Supplementary Fig. 9), SiO2@Ag-1, SiO2@Ag-2, and SiO2@Ag-4 (p<0.05, Fig. 2a–c, Supplementary Table 2). In addition, we viewed molecular peaks with Na+ adduction as well in Supplementary Fig. 10, including m/z of 205.28 [M+Na]+ for mannitol (Supplementary Fig. 10a), m/z of 203.26 [M+Na]+ for glucose (Supplementary Fig. 10b), and m/z of 172.21 [M+Na]+ for methionine (Supplementary Fig. 10c). We summarized typical m/z values of identified molecular peaks in Supplementary Table 3. In the above selection of optimized materials, the shell structure of SiO2@Ag was the key parameter and the differences in the physical and chemical properties of various small metabolites can lead to the different ionization efficiency as observed, even using the given SiO2@Ag in LDI MS analysis. SiO2@Ag-3 with a thin packed silver layer (Fig. 1) afforded structural stability, specific nano-gaps, and surface plasmon resonance, leading to significantly higher performance over the SiO2 and SiO2@Ag-1/2/4. Overloading of silver on the silica (SiO2@Ag-4) reduced the analytical efficiency of SiO2@Ag-3, owing to the surface aggregation of excess silver on the silica core (Fig. 1f, i; Supplementary Fig. 4b), rather than clumping of SiO2@Ag particles (Fig. 1g; Supplementary Fig. 11). Insufficient loading of silver (SiO2 and SiO2@Ag-1/2) also affected the analytical efficiency of SiO2@Ag-3 (Fig. 1e), due to more surface area from silica cores exposed to metabolites and less specific silver nano-crevices. Notably, the unique silver peaks (Ag1-3 +) can be used for mass calibration in each spectrum for accurate analytes identification.
In addition to the bare silver nanoshells, surface functionalization with molecular probes can lead to specific capture of molecules. As a demonstration, we enriched kanamycin using the aptamers-functionalized silver nanoshells and detected the molecular peaks at m/z of 507.18 [M+Na]+, 591.16 [M+107Ag]+, and 593.16 [M+109Ag]+ at low concentrations (from 200nM to 20μM, Supplementary Fig. 12). These results suggested that the silver nanoshells may serve as a volatile platform for selective and sensitive of capture and detection of target molecules.
We further studied the salt tolerance and protein endurance of designer silver nanoshells (SiO2@Ag-3) for small metabolites detection at low abundance, which can be fundamentally important for real-case applications in complex bio-fluids. Dealing with a mixture of four small metabolites (2nM each) in highly concentrated NaCl solution (0.5M) in Fig. 2d, we obtained Na+-adducted signals at m/z of 169.26 [M+Na]+ for lysine, 172.21 [M+Na]+ for methionine, 188.22 [M+Na]+ for phenylalanine, and 197.25 [M+Na]+ for arginine. In parallel, we obtained K+ adducted signals in 0.5M KCl solution (Supplementary Fig. 13). In a complex sample containing proteins (5mgmL−1 BSA) and salts (KCl solution, 0.5M), we can still observe clear silver ions-adducted signals (Fig. 2e) at m/z of 238.22 [M+107Ag]+ and 240.22 [M+109Ag]+ for leucine, 256.19 [M+107Ag]+ and 258.19 [M+109Ag]+ for methionine, 281.27 [M+107Ag]+ and 283.27 [M+109Ag]+ for arginine, and 287.22 [M+107Ag]+ and 289.22 [M+109Ag]+ for glucose, demonstrating the fine salt tolerance and multiplexity of SiO2@Ag-3 for practical use. The nano-crevice of silver nanoshells can trap small analytes and transfer laser energy for selective desorption/ionization process, whereas mass spectrometry served as the ideal tool to screen diverse metabolites simultaneously with high throughput.
The incidence of postoperative central nervous system infection after neurosurgical procedures is ~5–7 % globally and even higher if antibiotic prophylaxis is not applied in time, which significantly endangers the human health by damaging the nerve systems51, 52. CSF detection is decisive to identify patients with postoperative brain infection and glucose level in CSF has been affirmed as diagnosis criteria52, 53. However, present measurement glucose level in CSF for diagnostics uses the traditional biochemical method, encountering many problems including tedious sample pre-treatment, long time of biochemical reaction, large sample consumption (~200μL), and so forth. Using the designer silver nanoshells (within minutes for the overall experiments), we directly detected the Na+-adducted glucose at m/z of 203.26 (Fig. 3a) and Ag+-adducted glucose at m/z of 287.23 and 289.23 (Fig. 3b), together with mannitol (m/z of 205.28, 289.23, and 291.23) and other small metabolites (Supplementary Table 4), consuming 0.5μL of native CSF only. Besides the accurate mass measurement, we also performed MS/MS analysis of these signals and compared these results with tandem mass spectra from standards for identification (Supplementary Figs. 14–17).
It should be mentioned that LDI MS is usually used for qualitative or at most semi-quantitative analysis, and the MS peak intensity cannot be used for quantification owing to the non-predictable analyte-dependent ion production behavior. Thus, we coupled isotopic quantification to measure the concentration of glucose for diagnostic purpose in CSF, affording consistent calibration curves with coefficient of determination (R 2) of 0.99 using isotopes with six 13C (Fig. 3c) and using one 13C (Supplementary Fig. 18). Figure 3d showed one typical mass spectrum of analyte from five experiments (Supplementary Fig. 19 showing the other four) and its isotope with analyte/isotope (A/I) ratio of 1/1. Notably, compared to the biochemical method based quantification, the sample volume was reduced from 200 to 0.5μL and the experiment time was reduced from hours to several minutes by the LDI MS. The isotopic quantification afforded the average recovery of ~131% with coefficient of variation (CV) within 6% (Fig. 3c), which was comparable to the biochemical method that afforded the average recovery of ~107% with CV within 14% (Supplementary Fig. 20). We concluded that the silver nanoshells (SiO2@Ag-3)-assisted LDI MS achieved fast, sensitive, accurate, multiplex, quantitative, and reproducible detection of small metabolites in native CSF without any enrichment or purification.
We applied the silver nanoshells-assisted LDI MS to monitor the glucose level in patients CSF for diagnosis of postoperative brain infection. Different from blood systems, glucose level in CSF is more stable as an energy source for brain and has a vital role in neurotransmitter synthesis and synaptic neurotransmission54, 55. Postoperative brain infection causes low glucose levels in CSF due to the metabolism of operation introduced bacteria53, 56, and the threshold of glucose level is ~2.8–3.9mM in continued drained CSF as demonstrated in the earlier reports56, 57. As displayed in Fig. 3e for a patient with brain infection, the glucose levels were 2.83, 2.88, 3.16, and 2.71mM for consecutive 4 days after an operation, suggesting postoperative brain infection. For comparison, the as measured glucose levels of a patient without infection were 4.66, 6.12, 5.45, and 5.09mM using silver nanoshells-assisted LDI MS also for consecutive 4 days (Fig. 3f). Further, we detected 17 CSF samples from infected patients and 21 CSF samples from uninfected controls by our approach (Fig. 3g), which showed consistence with the current biochemical method used in hospitals affording coefficient of determination (R 2) of 0.92 (Supplementary Fig. 21, see also Supplementary Table 5 for details). We obtained the optimized diagnostic sensitivity of 81.6% with specificity of 88.2% based on the cut-off value of 2.85mM according to the receiver operating characteristic (ROC) curve (area under curve of ROC (AUC) of 0.961, Fig. 3h). Previous LDI MS approaches were mostly limited to standard analysis, spiked experiments, or imaging tests, considering the difficulties in sample pre-treatment of complex bio-fluids for enrichment and purification. In the above demonstration, we demonstrated clinical diagnosis of patients with postoperative brain infection as well as daily monitoring by advanced LDI MS analysis of CSF, using the silver nanoshells with designer structural parameters.
Pharmacokinetic study guides the overall therapeutics and has been applied universally in clinics during diverse medical treatment. It is instrumental to understand the distribution and metabolism of selected drugs for pharmacokinetic study. For instance, mannitol, a small metabolite, has been long employed for reducing intracranial pressure, and increasing cerebral perfusion and blood flow during clinical treatments58, 59. Nevertheless, monitoring concentrations of small metabolites/drugs (e.g., mannitol) in serum and CSF is difficult by traditional electrospray ionization (ESI) MS techniques due to the high samples complexity and low molecular abundance, which requires enrichment or separation (such as, chemo-selective extraction and liquid/gas chromatography) and is time-consuming, labor-intensive, and very expensive for large-scale use60–62. With 0.5μL of native serum, we directly obtained the Na+-adducted mannitol at m/z of 205.28 (Fig. 4a) and Ag+-adducted mannitol at m/z of 289.23 and 291.23 (Fig. 4b), together with glucose (m/z of 203.26, 287.23, and 289.23) and other small metabolite (Supplementary Table 6), based on the optimized silver nanoshells. We compared tandem mass spectra of these signals from bio-samples and standards, in addition to the accurate mass measurement for identification (Supplementary Figs. 14–17). As control experiments, we observed no signals by LDI MS without any matrix due to low LDI efficiency (Supplementary Fig. 22a). We obtained overwhelming background noises with few peaks from small metabolites using organic matrix (e.g., α-cyano-4-hydroxycinnamic acid, CHCA) in Supplementary Fig. 22b and can only recognize glucose signal using inorganic matrix (gold nanoparticles) in Supplementary Fig. 22c, both of which demonstrated the advantages of silver nanoshells over current matrices in bio-analysis.
For diagnostic purpose, we also applied isotopic quantification to measure the concentration of mannitol in serum. The calibration curve afforded a coefficient of determination (R 2) of 0.95 in a dynamic range of 100–600ngμL−1 (Fig. 4c) and high reproducibility in serum detection with a standard deviation of 1.311% for five independent tests (Supplementary Fig. 23). We also examined the stability of silver nanoshells for serum detection and found that SiO2@Ag can be capable of LDI MS analysis for at least 5 months (Supplementary Fig. 24). Notably, despite the high sample complexity of serum, we achieved efficient LDI MS analysis of small metabolites without any sample pre-treatment similar to that in CSF detection and validated the performance of the silver nanoshells (SiO2@Ag-3) in complex bio-fluids.
We monitored the concentration changes of mannitol in serum and CSF, and demonstrated distribution of mannitol in blood and CSF systems during therapeutic treatment of three patients with cerebral edema. Notably, the blood and brain/CSF systems are divided by the blood–brain/CSF-barriers, which can only allow specific molecules to pass63–65. In CSF samples analysis of three patients based on the silver nanoshells-assisted LDI MS, mannitol concentrations showed minor increase by ~29.41–128.96% before and 30min after intravenous injection during the therapeutic treatment (Fig. 4d; Supplementary Table 7, p<0.05). In contrast, the mannitol concentrations after drug delivery were significantly higher than untreated ones, showing ~45.23–53.35-folds increase in serum samples of the patients (Fig. 4e; Supplementary Fig. 25; Supplementary Table 7, p<0.05). The blood–brain/CSF-barriers are fundamentally important to maintain the normal physiological activity of living systems, whereas the molecular permeability of the barrier guides the use of drugs in clinical practice63–65. Our results demonstrated the application of silver nanoshells as matrix for LDI MS to investigate the function of blood–brain/CSF-barriers in dividing blood and brain/CSF systems, as well as the permeability enabling the pass of mannitol66, 67. Considering the advantages of silver nanoshells-assisted LDI MS over ESI MS that required sample pre-treatment61, 62 and LDI MS using other matrices (Supplementary Fig. 22), we anticipate our approach to engage the advanced pharmacokinetic study of various small metabolites and drugs for large-scale clinical use.
In summary, we introduced silver nanoshells (SiO2@Ag) as matrices for direct LDI MS detection of small metabolites in bio-fluids, and further developed a platform technology for metabolic analysis-based disease detection and therapeutic evaluation. We synthesized series of silver nanoshells with controlled structures by multi-cycled silver mirror reactions and selected the designer silver nanoshells with optimized analytical performance. We revealed the mechanism for efficient LDI process associated with structural parameters of materials, and demonstrated the application in complex bio-fluids in real cases. Furthermore, coupling with isotopic quantification of selected metabolites (e.g., glucose and mannitol), we not only identified patients with postoperative brain infection by CSF analysis, but also monitored the drug concentrations in both CSF and serum to investigate the blood–brain/CSF-barriers and for pharmacokinetics study. Our work contributes to the design of materials for high-performance metabolic analysis towards precision medicine and initiates the development of diverse advanced diagnostic tools involving various metabolic biomarkers.
Ammonium hydroxide (28–30%), tetraethyl orthosilicate (TEOS, 96%), ethanol absolute (99.7%), silver nitrate (99.5%), sodium chloride (99.5%), potassium chloride (99.5%), and trifluoroacetic acid (TFA, 99%) were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd (Beijing, China). Acetonitrile (ACN, 99%), α-cyano-4-hydroxycinnamic acid (CHCA, 99%), dithiothreitol (DTT, 99%), cetyltrimethylammonium chloride (CTAC, 99%), albumin from bovine serum (BSA, 98%), polyvinylpyrrolidone (PVP, MW=40,000), D-glucose (99.5%), L-methionine (99%), D-mannitol (99%), L-lactic acid (98%), L-arginine (99.5%), L-tryptophan (98%), uric acid (99%), and DL-phenylalanine (99%) were purchased from Sigma, USA. Tris base and phosphate-buffered saline (PBS, 10×, pH 7.4, cell-culture grade) were purchased from Yeason Biotechnology Co., Ltd (Shanghai, China). The isotopic internal standards of glucose (labeled with six 13C or one 13C) and mannitol (labeled with one 13C) were purchased from Cambridge Isotope Laboratories (CIL, USA). Thiol-aptamers were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). All aqueous solutions were prepared using deionized water (18.2MΩcm, Milli-Q, Millipore, GmbH) throughout the experiments.
SiO2@Ag core-shell particles were prepared through controlled surface coating of silver on silica nanoparticles. Smooth and uniform silica nanoparticles were synthesized as the hard templates using the Stöber method68. To perform the surface coating, the silver mirror reaction was used. Overall, 1.80g of the prepared silica nanoparticles was dispersed in 90mL of ethanol solution. In a typical reaction cycle, freshly prepared [Ag(NH3)2]+ ion solution (0.59M, 10mL) was quickly added to the above particles dispersion and sonicated at room temperature for 30min. Then the particles were mixed with 300mL of PVP ethanol solution (0.5mM). During the experiment, PVP was employed as both the stabilizer and reductant38. The mixtures were stirred at 30/50/70°C for 2/4/5/7h for the formation of silver nanoshells (SiO2@Ag). The structures of silver nanoshells can be adjusted by conducting the above reactions towards at the optimized condition (at 70°C for 7h, further increased temperature or time may cause unwanted precipitation) for 1–4 rounds to obtain SiO2@Ag-1/2/3/4. The resulting products were washed with 50mL of ethanol and deionized water and centrifuged at 10,000×g for 10min for three times. The final SiO2@Ag particles were dried at 60°C and stored as powders. The UV-Vis spectra of particles were checked and the qualified particles would show the absorption peak at ~436nm without red shift (suggesting no overloading of silver).
Gold nanoparticles were synthesized using the in-solution seeding approach16. The seeding solution was firstly prepared by vigorous mixing of 10mL of aqueous CTAC solution (0.1M) and 515mL of HAuCl4 (4.86mM) with 450mL of NaBH4 solution. Then the solution was aged for at least 1h in a hot bath and then was diluted for ten times. Next, 10mL of CTAC solution (0.1M) was mixed with 515mL of HAuCl4 (4.86mM) and 75mL of ascorbic acid (0.04M). Overall, 100mL of diluted seed solution was added into the above mixed solution under sonication and kept in darkness for 2 days to obtain the final gold nanoparticles after centrifugation and washing with water.
To modify silver nanoshells with aptamers, we used a post-grafting method23, 46. Briefly, 48μL of PBS buffer was added into 1 OD of SH-modified kanamycin aptamers (5′-SH-(CH2)6-TGGGGGTTGAGGCTAAGCCGA-3′) to a final concentration of 100μM. 0.48μL of DTT solution (10μM) was mixed with aptamer solution and incubated at 37°C for 1h. Then 200μL of SiO2@Ag-3 (0.5mgmL−1) was added to the system and gently shaken at room temperature for 24h with addition of NaCl (0.1M) after 12h. After incubation, the mixture was centrifuged for 30min at 13,000×g and re-dispersed in PBS.
TEM images, HRTEM images, and elemental mapping images were collected using a JEOL JEM-2100F instrument. Normally, ~8–10μL of ethanol suspension of materials was deposited onto a copper grid before observation. During sample preparation for elemental mapping, 1mgmL−1 of water dispersions of SiO2@Ag-3 were mixed with 10μgμL−1 glucose (1/1, V/V) for 30min and supernatant was discarded by centrifugation at 10,000×g for 10min. The precipitates were washed and re-dispersed in water and put onto micro-grids for analysis. The inter planar space was measured by Digital Micrograph version 2.5 software (Gatan). SEM images and EDX spectra were recorded on Hitachi S-4800 by dropping the materials suspensions on the aluminum foil. Room temperature optical absorption spectra of the materials were obtained on an AuCy UV1900 spectrophotometer. Fourier transform infrared spectroscopy (FTIR) was performed on Nicolet iN10 MX (Thermo Scientific, USA). Nitrogen adsorption isotherms were obtained on Micromeritics ASAP 2020M and the samples were degassed in vacuum before tests. Zeta potential and dynamic light scattering size measurements were performed on a Nano-ZS90 instrument in water at 25°C (Malvern, Worcestershire, UK).
For LDI MS detection of metabolites, standard small molecules (glucose, mannitol, methionine, tryptophan, uric acid, lactic acid, arginine, and phenylalanine) were dissolved in deionized water by step-wise dilutions with the concentration ranging from 1μgμL−1 to 1ngμL−1. Standard molecules were mixed with salts (NaCl, KCl, 0.5M) and proteins (BSA, 5mgmL−1) to explore the detection efficiency in complex samples. In a typical LDI MS experiment, SiO2@Ag particles and gold nanoparticles were dispersed in water at a concentration of 0.5mgmL−1 for matrix use. CHCA was dissolved in 0.1% TFA buffer (water/ACN, 50/50, v/v) at a concentration of 4mgmL−1. Then 500nL of matrix slurry was deposited with 500nL of analytes solution on a stainless steel target plate and dried for LDI MS analysis. Mass spectra were collected in the reflection mode employing delayed extraction on 5800 Proteomics Analyzer (Applied Biosystems, Framingham, MA, USA) with the Nd:YAG laser (1kHz 355nm). The repetition rate and an acceleration voltage were set as 200Hz and 20kV, respectively. The delay time for this experiment was optimized to 200ns. The number of laser shots was 200 per analysis for all LDI MS experiments. Only MS signals with signal-to-noise ratio over 10 were used for identification of molecules. Mass calibration was conducted by background substrate mass peaks of [Ag]n + (n=1–3) in each spectrum for accurate mass measurement. MS/MS of selected molecular peaks of glucose and mannitol (from bio-samples and standards) were performed and compared for identification purpose in addition to the accurate mass measurement. The mass accuracy was within 50ppm, which was comparable to previous reports69. No smoothing procedures were applied and all spectra were directly used for analysis.
For enrichment and detection of kanamycin, 50μL of aptamers modified silver nanoshells slurry was centrifuged and the precipitate was re-dispersed in tris–HCl buffer (pH 7.5, 20mM). Overall, 1mL of kanamycin with different concentrations (from 200nM to 20μM) was mixed with the dispersion above and incubated at 37°C for 1h. The mixture was centrifuged to discard supernatant and re-dispersed in 0.5μL of 0.1% TFA buffer (water/ACN, 20/80, v/v) for LDI MS analysis as established above.
The isotopes of selected metabolites were introduced as the internal standard for quantification use17. Standard solutions of small molecules with different concentrations were employed to plot the calibration curves. The isotopes were dissolved in water with concentrations of 250ngμL−1 and 100ngμL−1 for glucose and mannitol, respectively. Typically, the isotope solution was mixed with analyte solution with a volume ratio of 1:1. After dropping 500nL of mixture solution on the plate, 500nL of matrix solution was deposited onto it and dried for LDI MS analysis. The influence of naturally occurring 13C (12C/13C, 98.89%/1.11%) was eliminated by subtracting corresponding signal intensity during the quantification process using isotopes with one 13C. For quantification purpose, the relative intensity ratio of analytes/isotopes from five independent experiments was recorded with data shown as the mean±s.d. (n=5).
Glucose level in 200μL of native cerebrospinal fluid samples were detected using the traditional biochemical approach by the hexokinase/glucose-6-phosphate dehydrogenase assay (Abbott Diagnostics, Maidenhead, UK), where the glucose concentrations were obtained after enzymatic catalysis of glucose according to the colorimetric method.
All statistical analysis in this work (including materials characterizations and bio-analytical results) was performed based on the SPSS software (version 19.0, SPSS Inc., Chicago) to calculate the p-value for statistical demonstration, including independent sample t-test, paired t-test, one-sided t-test, and Wilcoxon signed-rank test.
CSF samples were donated by patients in Children’s Hospital of Shanghai. The CSF samples for brain infection monitoring were collected in four consecutive days during operation. For diagnostic application, 17 CSF samples were obtained from infected patients and 21 samples were obtained from uninfected controls. All the investigation protocols in this study were approved by the institutional ethics committees of the Children’s Hospital of Shanghai and School of Biomedical Engineering, SJTU. Informed consents from patients had been obtained since the project started. All samples were harvested in tubes by continued drainage without tedious lumbar punctures according to the standard clinical procedures and stored at −80°C until use9, 17, 70. The CSF samples were directly detected by the LDI MS using the silver nanoshells as matrix following the previous protocols in standard analysis and five independent experiments were performed with data shown as the mean±s.d. (n=5) for diagnostic use.
For pharmacokinetic study, both blood and CSF samples were donated by three patients with cerebral edema before and 30min after rapid intravenous injection of mannitol (20% infusion solution, Shandong Qidu Pharmaceutical Co., Ltd., China) during a surgical operation. All of the investigation protocols in this study were approved by the institutional ethics committees of the Children’s Hospital of Shanghai and School of Biomedical Engineering, SJTU. Informed consents from patients had been obtained since the project started. The harvesting of serum samples was completed by vein blood draws based on the established method9, whereas the sampling of CSF was the same as brain infection diagnosis experiments. The serum and CSF samples were directly detected by LDI MS using the silver nanoshells as matrix following the previous protocols in standard analysis and five independent experiments were performed with data shown as the mean±s.d. (n=5) for pharmacokinetic study.
The data that support the findings of this study are available from the corresponding author upon reasonable request.
We gratefully thank the financial support from Project 81401542 and 81650110523 by National Natural Science Foundation of China (NSFC), Project 16441909300 by Shanghai Science and Technology Commission. This work is also sponsored by the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning (TP2015015).
K.Q. foresaw this work and designed the overall approach with R.C., J.W., and L.H. L.H. carried out experiments and wrote the manuscript. L.H. and J.W. contributed equally to this work. X.W., Y.L., J.H., X.S., R.Z., D.D.G., and V.V. analyzed data and contributed to synthesis, functionalization, and characterization of materials and assisted the analytical experiments. J.W. and Y.L. supervised the MS analysis. All authors joined in the critical discussion and edited the manuscript.
The authors declare competing financial interest. The authors have filed patents for both the technology and the use of the technology to detect bio-samples.
Electronic supplementary material
Supplementary Information accompanies this paper at doi:10.1038/s41467-017-00220-4.
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