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Logo of ppjE-SubmissionArchivesInstructions to AuthorsThis ArticleThe Plant Pathology Journal
 
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Published online 2017 August 1. doi: 10.5423/PPJ.OA.03.2017.0047

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Subcellular localization of 126 kDa and movement protein of Pepper mild mottle virus (PMMoV) as influenced by the proportion of strain KBPF-004 supernatant ratio. Binary vector construct expressed from Agrobacterium was maintained at an equal concentration with each dilution of strain KBPF-004 culture supernatant. Four quadrants of each N. benthamiana leaf were infiltrated with mixtures of 1:1:0 (1 ml of binary vector expressed Agrobacterium (OD600: 0.6): 1 ml of ATCC 8062 supernatant: 0 ml of strain KBPF-004 supernatant), 1:0.8:0.2, 1:0.6:0.5, and 1:0:1, respectively. Confocal microscopy using a Zeiss LSM 710 detected green fluorescence with excitation at 488 nm from an argon laser, and emission monitored at 500–520 nm at 36 h after agroinfiltration. Scale bars = 50 μm.

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