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The transcriptome sequencing of melanoma cells from two mouse models differing in the expression level of the scaffold protein Receptor for activated C kinase (RACK1) are presented. Primary melanoma cells were harvested from Tyr:NRasQ61K; Pax3GFP/+ mice, with or without the Tyr:Rack1-HA transgene. Cells were cultured and infected with scramble shRNA or Rack1-targeting shRNA, on technical triplicates of viral infection. Libraries were prepared by selecting polyadenylated mRNAs and RNA Sequencing (RNASeq) was performed. Samples are described in the SRA portal (SRP096162) and FASTQ files have been deposited in Sequence Read Archive (accession numbers: SRR5150106 to SRR5150117). The interpretation of these data is presented in the following research article: “RACK1 cooperates with NRASQ61K to promote melanoma in vivo” (Campagne et al., 2017, doi: 10.1016/j.cellsig.2017.03.015) .
Data provided in this article correspond to the FASTQ files obtained after RNA sequencing of melanoma cells from two mouse models, treated with scramble shRNA or Rack1-targeting shRNA.
Melanoma cells were sampled from primary tumors of Tyr:NRasQ61K; Pax3GFP/+ mice with or without Tyr:Rack1-HA transgene. Cells were cultured as previously described , and RNA interference was performed with scramble shRNA (sequence GTCACTCACCCTTCGGTTATT) or a shRNA targeting exon 2 of Rack1 (sequence GGTCACTCCCACTTCGTTATT). Transduction was performed at 0.45 ng/µl of lentiviral titer in the presence of polybrene. RNAs were collected on the third day.
RNA samples were sequenced in an Illumina HiSeq. 2500 following manufacture׳s recommendations. In brief, libraries were prepared by selecting polyadenylated mRNA using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA). Samples were tagged with barcode sequences for subsequent identification, amplified by PCR and quantified by qPCR using the QPCR Library Quantification Kit (Agilent Technologies, Santa Clara, CA). 4 libraries were sequenced in paired-ends 2×100 bp in three Illumina lanes. The full data sets have been submitted to NCBI Sequence Read Archive (SRA) under Accession.
This work was funded by a grant from Institut National de la Recherche Agronomique (IPRM-RACK) (INRA- Crédits incitatifs du département de génétique animale) to GE.
Transparency documentTransparency data associated with this article can be found in the online version at doi:10.1016/j.dib.2017.07.006.