|Home | About | Journals | Submit | Contact Us | Français|
The p16/RB1 tumor suppressor pathway is inactivated in the vast majority, if not all, human cancers. The current paradigm is that p16 and RB1 function in a linear pathway to suppress tumorigenesis; however p16 is preferentially lost in human cancers suggesting that p16 has critical tumor suppressive functions not mediated through RB1. Carcinomas arise from transformed epithelial cells and account for 80% of adult malignancies highlighting the need to understand p16/RB1 pathway function in organ epithelia. Lung cancer is the leading cause of cancer deaths and is associated with p16/RB1 pathway deregulation. We demonstrate that p16 is upregulated in the lung epithelium after Rb1 ablation in genetically engineered mouse models. In contrast to fibroblasts, loss of RB1 family proteins, p107 or p130, did not result in p16 induction, demonstrating that p16 suppression is a unique RB1 pocket protein function in the lung epithelium in vivo. p16 upregulation did not induce cellular senescence but rather promoted survival of RB1-deficient lung epithelial progenitor cells. Mechanistic studies show that p16 protects RB1-deficient cells from DNA damage. Consequently, additional loss of p16 led to genetic instability and increased susceptibility to cellular immortalization and transformation. Mice with combined RB1/p16-deficient lungs developed lung tumors including aggressive metastatic lung cancers. These studies identify p16 loss as a molecular event that causes genetic instability and directly demonstrate that p16 protects against DNA damage in the absence of RB1 function providing an explanation for why p16 is preferentially targeted in human cancers.
The tumor suppressor proteins p16 and RB1 function in a common pathway that is deregulated in the vast majority of human cancers.1, 2 The current paradigm is that p16 and RB1 function in a linear pathway wherein p16 blocks cell cycle progression by maintaining RB1 in a functionally active state by inhibiting Cyclin D/Cyclin-dependent kinase 4/6 dependent phosphorylation.3 As p16 loss and Rb1 mutations both lead to loss of RB1 function, it is unclear why p16 and Rb1 are targeted in specific cancers and why p16 loss occurs much more frequently and in a wider variety of cancer types than does Rb1 mutation. Deletion of the p16 locus is the most frequent copy number alteration across 12 commonly occurring cancers types, and p16 is among the genes most frequently silenced by methylation.1 In contrast, Rb1 mutations are only frequently detected in retinoblastoma and small cell lung cancer (SCLC).4, 5 The markedly increased frequency of p16 as compared to RB1 loss in human cancers suggests that p16 has critical tumor suppressive functions that are not mediated through RB1.
The p16/RB1 tumor suppressor pathway is deregulated in virtually all lung cancers providing strong evidence that loss of p16/RB1 pathway function is required for lung carcinogenesis.4, 6, 7, 8 Lung cancer is the leading cause of cancer related deaths and has a dismal overall 5 year survival rate of <20%.9 Lung cancers are divided into non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC) with p16 loss being detected in up to 80% of NSCLC and biallelic Rb1 loss being obligatory for development of SCLC.4, 6, 7, 8 Previous studies by us and others demonstrate that RB1 loss targeted to the murine lung epithelium results in neuroendocrine cell hyperplasia with additional Trp53 loss being sufficient for progression to SCLC, an aggressive neuroendocrine malignancy.10, 11, 12 These results in mouse models are in accordance with the obligatory loss of RB1 and TP53 in human SCLC providing evidence that genetic mechanisms underlying lung carcinogenesis are conserved between mice and humans.4 Despite the frequent loss of p16 in human NSCLC, however, p16 or RB1 loss alone or in combination with Trp53 in genetically engineered mice is not sufficient for development of NSCLC. We previously demonstrated that p16 is induced after RB1 ablation in lung epithelial progenitor cells, namely Club and type II cells, believed to serve as cells of origin for NSCLC.11, 13, 14 Increased p16 expression is also reported after knockdown of RB1 or its family members, p107 (RB1l1) or p130 (RB1l2), in human fibroblasts in culture as well as being a hallmark of human papilloma virus-driven cervical and head and neck cancers wherein RB1 family function is lost due to E7 viral oncoprotein expression.15, 16, 17 Induction of p16 promotes cellular senescence to limit tumorigenesis with maintenance of senescence believed to be heavily reliant on active hypophosphorylated RB1.18 However, RB1 loss in the thyroid induces cellular senescence with additional loss of p16 promoting tumor progression.19 These results suggest that p16 associated cellular senescence antagonizes RB1-deficient carcinogenesis and provide evidence that p16 has tumor suppressive functions that are not mediated through RB1. In the current study, genetically engineered mouse models were used to determine the regulation and biologic significance of p16 induction in RB1-deficient lung epithelial cells that give rise to lung cancer; a common epithelial derived malignancy. We demonstrate that p16 suppression in the lung epithelium in vivo is a unique RB1 function, differing from the shared p107 and p130 function in fibroblasts.15 We also show that unlike in murine and human fibroblasts, RB1 loss in lung epithelial progenitor cells is sufficient to enhance growth providing evidence that p16/RB1 pathway function is distinct in epithelial cells.20, 21, 22 Importantly, p16 induction after RB1 loss was not associated with cellular senescence but rather protected lung epithelial progenitor cells from DNA damage and development of aggressive lung cancers. Together these studies directly demonstrate that p16 has tumor suppressive functions that are not mediated through RB1 and are critical for protecting against carcinogenesis.
Individual knockdown of RB1, p107 or p130 in cultured human fibroblasts results in p16 induction.15 In contrast, we demonstrate that suppression of p16 expression in the lung epithelium in vivo is unique to RB1. p16 protein and messenger RNA levels were increased in RB1-deficient lungs in a conditional mouse model wherein Rb1 ablation was targeted to the lung epithelium, but were not induced in p107−/− or p130−/− lungs (Figures 1a–c).11, 12 Increased p16 expression occurred by 4–5 weeks of age with elevated p16 protein levels being maintained in 8–9-month-old lungs (Figure 1b). Previous studies localized p16 protein expression to conducting airway Club cells and distal alveolar epithelial type II cells; two progenitor cell types believed to be cells of origin for NSCLC.11, 13, 14 p16 message was induced ninefold in RB1-deficient primary type II cell isolates as compared to RB1-proficient control cells consistent with RB1 mediating suppression of p16 at the transcriptional level (Figure 1c). In contrast, induction of p16 messenger RNA was not seen in p107−/− or p130−/− primary type II cells (Figure 1c). Together, these data demonstrate that p16 repression is a unique RB1 function in the lung epithelium and identify a fundamental difference between p16/RB1 pathway regulation in lung epithelial cells in vivo and fibroblasts in culture. Given that RB1, but not p107 or p130, is a bona fide tumor suppressor, we tested the hypothesis that the unique p16 upregulation after RB1 loss functioned to regulate epithelial cell growth and protect against carcinogenesis.
Similar to activated oncogenes, loss of tumor suppressor expression activates senescence to limit tumorigenesis in vivo.23 Senescent cells rely heavily on persistent p16 expression to maintain the senescent state.18 RB1 loss in the thyroid results in cellular senescence with p16 induction being important for antagonizing thyroid carcinogenesis.19 However, in contrast to the thyroid, cellular senescence was not induced in RB1-deficient lung epithelial cells despite robust p16 expression. Senescence-associated β-galactosidase activity, the most widely accepted biomarker of cellular senescence, was not detected in the RB1-deficient lung epithelium (Figure 1d) differing from the detection of this biomarker in the RB1-deficient thyroid.18, 19, 23 Exit from the cell cycle is the central characteristic of cellular senescence defined as a stress-induced irreversible proliferative arrest with resistance to mitogenic and oncogenic stimuli.18, 23 Consistent with p16 induction not being associated with cellular senescence, RB1-deficient lung epithelial cells proliferated in primary cultures and after cytotoxic injury in vivo to regenerate the pulmonary epithelium (Figure 1e).24 Taken together, these data indicate that p16 induction is not sufficient to induce cellular senescence in the RB1-deficient lung epithelium, and unlike in the thyroid is not associated with a cellular senescence state.
To directly assess the biologic significance of p16 induction in RB1-deficient epithelial cells, we generated mice with RB1-deficient lung epithelium in a p16−/− background and compared the growth of combined RB1/p16-deficient primary epithelial type II progenitor cells to control RB1/p16-proficient cells as well as cells lacking RB1 or p16 alone. RB1 loss resulted in increased epithelial cell growth as compared to RB1 and p16-proficient control cells (Figure 1e). This is in direct contrast to murine and human fibroblasts wherein RB1 family proteins have redundant functions with loss of all three pocket proteins being required to increase fibroblast growth.20, 21, 22
Based on the well-established growth suppressive functions of p16, it was expected that p16 loss would enhance RB1-deficient cell growth. Surprisingly, loss of p16 suppressed growth of RB1-deficient epithelial cells demonstrating that p16 was required for growth of RB1-deficient lung epithelial cells. Cells from littermate p16−/− lungs lacking one or both transgenes required for Rb1 ablation had growth rates similar to control cells indicating that p16 loss alone did not alter lung epithelial cell growth. Taken together these data identify a novel growth-enhancing function for p16 in RB1-deficient cells. Moreover, the data demonstrate fundamental differences in p16/RB1 pathway mediated control of epithelial versus fibroblast cell growth highlighting the importance of defining p16 functions in epithelial cells that give rise to cancer.
To identify the mechanism underlying p16-dependent promotion of RB1-deficient epithelial cell growth, cell cycle analysis was performed on primary lung epithelial type II cells of the defined genotypes. RB1 loss was associated with a slight increase in proliferating S-phase cells that did not reach statistical significance (Figure 2a) indicating that enhanced proliferation alone did not account for the increased growth of RB1-deficient epithelial cells (Figure 1e). Instead, enhanced RB1-deficient cell growth was due to increased cell survival that was dependent upon p16 (Figure 2b). Accordingly, additional loss of p16 led to increased death of RB1-deficient cells back to control levels (Figure 2b). p16 loss alone has no effect on lung epithelial cell survival but resulted in an altered cell cycle profile with a decreased proportion of cells in S-phase and more cells in G1 and G2/M (Figures 2a and b). The cell cycle profile alterations in p16−/− cells were independent of RB1 status and likely reflect changes in cell cycle kinetics given that p16 loss alone did not alter lung epithelial cell growth as assessed by cell number (Figure 1e). Taken together, the data demonstrate that RB1 and p16 loss have distinct effects on lung epithelial cell growth and that p16 functions to enhance growth of RB1-deficient lung epithelial cells. Moreover, the studies identify enhanced cellular survival as the mechanism by which RB1 loss and p16 promote lung epithelial cell growth.
Primary epithelial type II progenitor cells that serve as cells of origin for lung cancer were isolated from RB1/p16 deficient, p16 deficient, RB1 deficient and control RB1/p16-proficient lungs and serially passaged in culture using a modified 3T3 protocol to determine the role of RB1 and p16 in suppressing epithelial immortalization. Despite decreased growth of combined RB1/p16-deficient cells in short-term primary cultures, p16 loss resulted in increased immortalization of RB1-deficient lung epithelial progenitor cells in long-term culture. Twenty-five immortalized cell populations were established from RB1/p16-deficient cells, whereas only one immortalized RB1-deficient cell population that retained p16 expression was established, and no immortalized cell populations were obtained from p16−/− or control RB1/p16-proficient cells (Figure 3a). Primary type II cells of all genotypes underwent a progressive decline in growth within the first 2–3 successive transfers with the first sign of emergence as established lines being an increase in growth rate resulting in cell numbers exceeding that in the original culture. The growth rate has remained constant or continued to increase in the 10 cell populations that have been propagated for >10 passages to date, including two established cell lines propagated for >60 passages. Recombination of the floxed Rb1 allele and expression of the lung epithelial cell marker, Nkx2-1, was detected in all established cell populations confirming the lung epithelial cell lineage (Figures 3b and c). Interestingly, 80% (4/5) of the immortalized cell populations established from Rb1-deficient lungs lacked p16 messenger RNA and protein expression (Figure 3a) providing further evidence that p16 is critical for suppressing immortalization of RB1-deficient epithelial cells.
TP53 mutations are commonly detected in human cancers, including lung cancer, and spontaneous cellular immortalization is frequently associated with loss of TP53 and/or p19Arf.25 In contrast to other cell types, immortalization of RB1/p16-deficient cells was not associated with loss of Trp53 or p19Arf (Figure 3a and Supplementary Figure). Activated Trp53 as assessed by serine 15 phosphorylation was detected in 23 independently derived immortalized cell populations demonstrating maintained RB1/p16-deficient lung epithelial progenitor cell growth even in the presence of Trp53 activation (Supplementary Figure). Trp53 exome sequencing of 23 distinct cell populations detected a single missense G1187C mutation in exon 10 in one cell population resulting in the amino acid change, A344P (A347P in human; see Supplementary Figure and Supplementary Table). This deleterious missense mutation inhibits tetramer formation and lacks transcriptional activity but is infrequently mutated in human tumors being reported in only a rare liver cancer in the International Agency for Research on Cancer TP53 database.26, 27 Taken together, these data demonstrate that p16 suppresses immortalization of RB1-deficient lung epithelial progenitor cells, and that epithelial immortalization after p16 loss does not require loss of p19Arf or Trp53.
A unifying mechanism whereby p16 could enhance cell growth and protect from immortalization is by maintaining DNA integrity. Accumulation of DNA damage after p16 loss would lead to genetic instability resulting in cellular growth arrest or apoptosis if unrepaired while also predisposing to immortalization by increasing risk for acquiring additional oncogenic mutations. Indeed, p16 loss led to increased DNA damage in primary and immortalized RB1-deficient lung epithelial progenitor cells. Expression of the robust DNA damage marker, γ-H2AX, was significantly increased in combined RB1/p16-deficient primary lung epithelial type II cell isolates (Figure 4a).28 In addition, p16-proficient lung epithelial cells incurred less DNA damage than p16-deficient cells when grown in culture as well as after treatment with the DNA damaging chemotherapeutic drug, bleomycin, as assessed by γ-H2AX expression and comet assay (Figures 4b–d).
Protection from DNA damage is expected to enhance cell growth and suppress cellular transformation. Indeed, p16-proficient cells had markedly reduced sensitivity to bleomycin-induced growth arrest as compared to p16-deficient cells (Figures 5a–d). Bleomycin treatment inhibited growth of p16-deficient cell populations in a dose-dependent manner, whereas p16-proficient cells had markedly less growth suppression at the same bleomycin doses, indicating reduced sensitivity to chemotherapy-induced DNA damage. Enhanced growth of p16-proficient cells resulted from increased cell cycle progression as indicated by p16 loss leading to decreased S-phase and increased G2/M phase cells without significant alterations in apoptotic cell death (Figures 5c and d). Thus, p16 promotes growth of RB1-deficient lung epithelial progenitor cells by protecting against DNA damage resulting in enhanced cellular proliferation and growth.
To test whether increased DNA damage in p16-deficient cells predisposes to cellular transformation, p16-deficient and -proficient RB1 ablated lung epithelial progenitor cells were subcutaneously injected into the flanks of nude mice and assessed for tumor growth. Tumors developed in 22% (7/32) of sites injected with p16-deficient cells whereas no tumors developed at sites injected with cells that retained p16 expression. Tumors developed in four distinct mice representing two independently derived p16-deficient cell populations, and arose after 9–28 week latency periods consistent with the tumors arising as a result of randomly acquired oncogenic mutations. The tumors were comprised of highly pleomorphic epithelioid, spindled and giant cells with aggressive histologic features including invasion of surrounding adipose tissue and skeletal muscle with focal tumor necrosis (Figures 6a and b). The p16-deficient tumors were morphologically similar to human sarcomatoid lung carcinomas, a poorly differentiated NSCLC subtype associated with a worse prognosis.29
The role of p16 in suppressing spontaneous tumor formation in RB1-deficient lungs was investigated in vivo by generating double transgenic Scgb1a1-rtTA/tetCre mice wherein RB1 ablation was targeted to the lung epithelium in a p16+/− and p16−/− background (Figure 1a). The Scgb1a1 promoter was chosen for these studies to avoid the high incidence of bilateral medullary thyroid carcinomas that develop in mice with Sftpc driven RB1 ablation due to the targeting of a subset of thyroid cells.30 The incidence of spontaneous lung tumors was nearly doubled in p16-deficient mice with tumors having a more aggressive phenotype as indicated by increased cytologic atypia as well as invasive growth and metastases which were not seen in p16-proficient mice (Table 1 and Figures 6c–j). Multifocal lung tumors of multiple histologic subtypes developed in p16-deficient mice including SCLC which were never seen or reported in mice with RB1 or p16 loss alone or in RB1/p16-proficient controls (Table 1).11, 24, 31, 32, 33 Aggressive sarcomatoid carcinoma with chest wall invasion and metastatic nodules involving the diaphragm developed in a p16-deficient mouse that was morphologically indistinguishable from human sarcomatoid NSCLC and the subcutaneous tumors that developed from RB1/p16-deficient cells (Figures 6a–e). The incidence of multifocal tumors was increased in p16-deficient mice with two mice developing extensive multifocal adenomas and adenocarcinomas that nearly completely replaced the lung lobes with one developing metastasis to a mediastinal lymph node (Table 1 and Figures 6g–j). In contrast, tumors that developed in p16-proficient mice consisted of one or two localized tumors that lacked significant cytologic atypia and did not metastasize (Figures 6k–n). Small cell tumors only developed in combined RB1/p16-deficient lungs (Table 1). Two mice developed metastatic SCLC with mediastinal, chest wall, diaphragm and/or thoracic soft tissue metastases (Figure 6f). Small cell tumors were positive for the neuroendocrine marker, calcitonin related peptide alpha/calcitonin gene-related peptide (CALCA/CGRP), and negative for non-neuroendocrine type II and Club cell markers, SFTPC and SCGB1A1, respectively (Figure 6o). In contrast, the non-small cell lung tumors were positive for SFTPC and negative for CALCA and SCGB1A1, indicating a type II cell phenotype (Figure 6o). Lung tumors were present in mice analyzed at 17–21 months of age indicating a relatively long latency period consistent with p16 loss leading to sporadic accumulation of somatic oncogenic mutations as a result of increased susceptibility to DNA damage. Taken together, these data demonstrate that p16 induction after RB1 loss protects against DNA damage, cellular immortalization and transformation as well as development of aggressive lung tumors.
Loss of RB1 function is considered requisite for cancer development leading to intensive study of the roles and regulation of RB1 in controlling cell cycle progression.34 The RB1 family proteins, RB1, p107, and p130, have overlapping and compensatory functions in cell-cycle control. Like RB1, both p107 and p130 associate with E2F transcription factors to transcriptionally suppress genes required for cell cycle progression, interact with tumor virus oncoproteins, and induce cell-cycle arrest when overexpressed.35 Yet, despite the structural and functional similarities among the RB1 family proteins, somatic mutations in human cancers are almost exclusively found in Rb1 providing evidence that RB1 has unique functions in epithelial cells critical for suppressing carcinogenesis.34 Importantly, our studies demonstrate that RB1 loss alone is sufficient to drive lung epithelial progenitor cell growth which is in marked contrast to fibroblasts wherein loss of all three RB1 family proteins is required to promote fibroblast growth.20, 21, 22 Suppression of RB1, but not p107 or p130, also extends the proliferative lifespan of primary mammary epithelial cells providing further evidence that RB1 has unique growth suppressive functions in epithelial cells that are not shared in fibroblasts.36 We also show that p16 suppression is a non-redundant RB1 function in lung epithelial cells in vivo that again is distinct from fibroblasts in culture wherein all three RB1 family proteins suppress p16 expression.15 Thus, these studies identify critical differences in RB1 family protein function and p16 regulation in epithelial cells and fibroblasts, highlighting the importance of defining p16/RB1 tumor suppressive functions in epithelial cells that give rise to 80% of human adult malignancies.
The current linear paradigm that p16 functions by activating RB1 would predict that p16 induction in RB1-deficient cells is biologically inconsequential. Our results, however, demonstrate that p16 is biologically active in RB1-deficient lung epithelial cells. Notably, induction of p16 in RB1-deficient lung epithelium did not induce senescence but rather promoted cell survival and protected against DNA damage. Our data in combination with reports in the literature clearly demonstrate that p16 function in RB1-deficient epithelial cells is dependent upon cellular context. Similar to lung epithelial cells, p16 is critical for survival of RB1-deficient human papilloma virus-positive cervical cancer cell lines.37 However in contrast to the lung, p16 induction in the RB1-deficient thyroid is associated with cellular senescence and decreased epithelial cell growth. Interestingly, p16 protects against genetic instability to enhance survival and proliferation of hematopoietic progenitor cells demonstrating that this p16 function is not restricted to the lung.38 Loss of the telomere DNA-binding protein, protection of telomere 1 (POT1), results in DNA damage leading to fatal bone marrow failure.38 Induction of p16 in hematopoietic progenitor and stem cells in POT1 deficient mice protected against dysfunctional telomere induced DNA damage resulting in increased lifespan due to increased hematopoietic progenitor cell survival and proliferative capacity. Consequentially, loss of p16 resulted in genetic instability and ectopic apoptotic cell death similar to that seen in the current studies. Thus, unlike in the thyroid and in aging tissues wherein p16 accumulation results in cellular senescence, p16 accumulation in the setting of oncogenic events like RB1 loss, human papilloma virus infection and telomere dysfunction has important cytoprotective functions.39, 40, 41, 42 Together, these findings highlight the importance of taking into account the cellular context when interpreting physiologic functions of p16. In addition, the data directly demonstrate that p16 is biologically active in RB1-deficient lung epithelial cells, functioning to protect against DNA damage and cell death through mechanisms that do not require RB1.
p16 positively regulates all three Rb family proteins raising the possibility that p16 effects in RB1-deficient cells are mediated through p107 and/or p130. The phenotypes of RB1/p16 lungs in the current studies, however, differ markedly from phenotypes in combined RB1 family deficient lungs demonstrating that p16 effects cannot be explained by loss of RB1 family function alone. Ablation of all three RB1 family proteins in the lung epithelium leads to development of neuroendocrine tumorlets that do not progress to SCLC and no NSCL tumors.43 Thus, additional oncogenic events are required for development of the SCLC and NSCL tumors present in RB1/p16-deficient lungs. Combined RB1/p130 ablation using the same murine model as in the current studies was not sufficient to induce lung tumors.11 In addition, combined RB1/p107 loss led to an increased incidence of NSCL tumors that lacked the aggressive metastatic phenotype of RB1/p16-deficient tumors and was not sufficient to induce SCLC.11 Taken together these studies indicate that p16 effects in RB1-deficient cells cannot be solely explained by loss of RB1 family function. Thus, the current genetically engineered mice represent a tractable model to identify these critical p16 tumor suppressive functions that are not mediated through RB1.
While loss-of-function RB1 mutations are universally detected in retinoblastoma and SCLC, RB1 is much more frequently inactivated in human tumors by p16 loss providing evidence that p16 also has critical tumor suppressive functions that are not mediated through RB1 in humans. In the lung, the p16 locus is inactivated in 43% of adenocarcinomas and 72% of squamous cell carcinomas that comprise the vast majority of NSCLC.7, 8 Molecularly targeted therapies have dramatically improved treatment for NSCLC patients whose tumors have somatically activated oncogenes such as epidermal growth factor receptor (EGFR), translocated anaplastic lymphoma kinase (ALK), and serine/threonine-protein kinase B-Raf (BRAF).13, 44 However, there are currently no targeted therapies for the frequent tumors with p16 inactivation highlighting the need to define critical p16 tumor suppressive functions in the lung epithelium to guide rationale development of new therapeutic strategies. We demonstrate that p16 functions to protect against DNA damage in lung epithelial progenitor cells that give rise to NSCLC. Genetic instability, as was seen in lung epithelial cells after p16 loss, is a defining characteristic of cancer that promotes tumor initiation and disease progression.45 These findings are of direct relevance to human lung cancers wherein p16 loss occurs as an early event in carcinogenesis and is positively associated with dose and duration of exposure to tobacco smoke, a potent mutagen.46 Over 80% of lung cancers are associated with smoking and thus occur in a mutagenic environment wherein susceptibility to genetic instability would significantly increase cancer risk. SCLC and NSCLC are both associated with smoking and developed after p16 loss providing evidence that p16 functions to suppress tumorigenesis through RB1 independent mechanisms in epithelial cell lineages that give rise to both neuroendocrine and non-neuroendocrine cancers.13, 47, 48 Although p16 loss is not detected in human SCLC, these studies indicate that mechanisms underlying p16-dependent protection against DNA damage and carcinogenesis are shared in epithelial cells that give rise to both NSCLC and SCLC. Thus the molecular mechanisms underlying p16 protection of genetic stability may have general relevance to lung carcinogenesis and response to DNA damaging therapeutics.
In summary, the current studies directly demonstrate that p16 suppresses carcinogenesis through mechanisms that do not require RB1 function. Induction of p16 in RB1-deficient lung epithelial cells is not associated with cellular senescence as reported in other tumors and with cellular aging, but rather is critical for maintaining genomic integrity. Consequently, p16 loss results in epithelial cell death while also predisposing to cellular immortalization and transformation after a long latency, consistent with p16 protecting against DNA damage and subsequent accumulation of oncogenic mutations. The RB1 independent tumor suppressive functions of p16 are physiologically relevant in vivo as demonstrated by the development of aggressive lung cancers upon p16 loss in RB1-deficient lungs of genetically engineered mouse models. Together, these studies identify p16 loss as a molecular event that causes genetic instability in addition to inactivating RB1 function thus providing an explanation for why p16 is preferentially targeted in human cancers.
Sftpc-rtTA/tetCre Rb1f/f, Scgb1a1-rtTA/tetCre Rb1f/f and p16−/− mice were mated to generate the indicated genotypes. RB1 ablation was induced by doxycycline treatment and genotypes confirmed as previously described.11, 12, 24, 32
Primary type II cell cultures were isolated from lungs of 5–6-week-old mice, plated on transwell clear polyester membrane inserts covered with Cultrex basement membrane (Trevigen, Gaithersburg, MD, USA) in 6-well plates (Corning, Corning, NY, USA) and grown in Bronchial Epithelium Growth Medium (BulletKit, Lonza, Walkersville, MD, USA) supplemented with Penicillin/Streptomycin 50 × solution (1:100).49 Media was changed each day for the cell growth and cell cycle profile studies. Cells were removed with Dispase (BD Pharmingen, San Jose, CA, USA). Immortalization was assessed using a modified 3T3 protocol plating 3.5 × 103 cells per well in 12-well plates and passaging every 3 weeks with trypsin.50 The entire cell population was plated in subsequent passages if total cell count was 3.5 × 103 cells and media was changed without trypsinization in wells with 20 cells on visual inspection. Cells were grown in RPMI-1640 media supplemented with 10nM Hydrocortisone, 0.005mg/ml Insulin, 0.01mg/ml Transferrin, 10nM Beta-estradiol, 30nM Sodium selenite (HITES), 10 nM HEPES, 2nM L-glutamine, 100U/ml Penicillin, 0.1 mg/ml Streptomycin and 5% FBS for 33 weeks. Media was changed every week or more frequently if needed. Cells from an individual well were considered independently derived immortalized cell populations when cells emerged with constant or rising growth after a marked initial decline in growth that in all cases except one resulted in total cell numbers below the initial 3.5 × 103 cells plated. Immortalized cell populations could also be preserved by freezing with continued propagation after reestablishment in culture. Cellular transformation was assessed by subcutaneous injection of 4.1–5.3 × 106 cells into the flanks of <15 week-old female athymic NCr-nu/nu mice (Comprehensive Mouse and Cancer Core at Cincinnati Children’s Hospital Medical Center) and monitoring for tumor formation. Cell growth was determined by WST1 assays (Millipore, Bedford, MA, USA). Bleomycin treatments consisted of seeding 96-well plates (5 × 103 cells/well) and treating with bleomycin the following day. Untreated or dimethyl sulfoxide vehicle-treated cells served as controls.
Western blot analysis was performed as described11 with nitrocellulose membranes probed for p16 (m156, 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phosphorylated p53 (9284, 1:2000, Cell Signaling, Danvers, MA, USA), total p53 (CM5, 1:2000, Leika, Biosystems, Richmond, IL, USA), phospho-histone γ-H2AX (1:1000, Upstate, Cell Signaling Solutions, Temecula, CA, USA), p19ARF (sc-32748, 1:500, Santa Cruz Biotechnology), TUBA1B (DM 1A, 1:2500, Sigma-Aldrich Chemical Company, St Louis, MO, USA), GAPDH (8884, 1:10000, Cell Signaling) and/or ACTB (A5060, 1:1000, Sigma-Aldrich Chemical Company).
Tissues were analyzed using established protocols11 and antibodies CALCA (1:10000; Sigma-Aldrich Chemical Company), SFTPC (pro-SPC; 1:3000, Seven Hill; Cincinnati, OH, USA), SCGB1A1 (1:2000, Santa Cruz, Biotechnology).
RNA was isolated using Qiagen RNeasy plus Mini Kit. Real-time reverse transcription PCR was performed and analyzed as described.11 Taqman primers included assay probe set Nkx2-1, Mm004923451_m1, and p16 specific custom set Forward-CAACGCCCCGAACTCTTTC, Reverse-AAGAGCTGCTACGTGAACGT and Probe-CCCGATTCAGGTGATGAT.
Lungs were infused with a 1:1 mix of OCT compound and 30% sucrose/PBS/2mm MgCl2. Lung lobes were bisected, immersed in OCT and flash frozen in cryomolds. Frozen 5μm sections were fixed in 0.2% paraformaldehye in PBS for 10min, washed thrice and immersed into SA-ß-gal staining solution for 18h at 37°C. Slides were washed with PBS/2mm MgCl2 and counterstained with nuclear fast red. SA-ß-gal staining solutions: 40mm citric acid/Na2HPO4 buffer (adjusted accordingly for pH 6.0 or pH 4.0), 150mm NaCl, 2mm MgCl2, 5mm Potassium Ferricyanide, 5mm Potassium Ferrocyanide and 1mg/ml X-gal.
Cell cycle profiles were assessed by flow cytometry after labeling for 18h (primary cells) or 3h (immortalized cells) with 10μm bromodeoxyuridine (BrdU, BD Pharmingen) and staining with fluorescein isothiocyanate-conjugated anti-BrdU antibody and 7-AAD (BD Pharmingen). Cell death was determined by quantifying subG1 fractions and fluorescein isothiocyanate Cleaved caspase 3-stained cells (BD Pharmingen). Analysis was performed on a BD FacsCanto and data analyzed on FlowJo software.
Cells were trypsinized, resuspended in 4% FBS in PBS (3 × 105 cells/ml), embedded in liquefied 0.75% low melting point agarose and transferred onto slides. After solidification at 4°C, cells were placed in lysis (2.5m NaCl, 100mm EDTA, 10mm Tris Base and 1% sodium lauryl sulfate) and alkali (200mm NaOH and 1mm EDTA) solutions followed by electrophoresis in alkali solution for 15min at 1V/cm. After staining with SyBR Green solution, comet tails were photographed using Olympus IX60 System Microscope (Olympus, Pittsburgh, PA, USA) and Image ProPlus 7 software and measured using CometScore software. Tail moments were defined as percentage DNA in the comet tail multiplied by tail length.
Student t-test and one-way ANOVA followed by Tukey’s multiple comparisons or Dunnett’s test was used to analyze statistical significance between two groups or multiple groups, respectively. Fisher’s exact test was used for analyzing genotypic differences in cellular immortalization and transformation. Significance was indicated by P<0.05. Statistical tests were performed using GraphPad Prism version 6.07 for Windows and GraphPad Software (La Jolla, CA, USA).
We thank Xiaolan Ma for assistance with animal studies, Melissa Callahan for assistance with genotyping, Eric Smith and Susanne Wells for technical expertise and reagents for comet assays, Veterinary Services for excellent animal care, and the Pathology Research Core, Research Flow Cytometry Core in the Division of Rheumatology supported in part by National Institutes of Health (NIH) grants AR-47363, NIH DK78392 and NIH DK90971, Comprehensive Mouse and Cancer Core, and DNA Sequencing and Genotyping Facility at Cincinnati Children’s Hospital Medical Center for technical expertise and assistance.
MS, DSS and KAW-B designed the research; MS, NA, AR, ALM and DSS performed the experiments; MS, NA, AR, ALM, DSS and KAW-B analyzed the data; and MS and KAW-B wrote the manuscript with review and suggestions provided by the remaining authors.
Supplementary Information accompanies this paper on the Oncogenesis website (http://www.nature.com/oncsis)
This work was supported by NIH grants HL079193 (to KAW-B) and F30 HL097609 (to DSS), and American Cancer Society grant RSG-10-194-01-TBG (to KAW-B).