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Logo of arthrestherBioMed Centralbiomed central web sitesearchsubmit a manuscriptregisterthis articleArthritis Research & Therapy
 
Arthritis Res Ther. 2004; 6(5): R404–R414.
Published online Jul 19, 2004. doi:  10.1186/ar1205
PMCID: PMC546278
A proinflammatory role for Fas in joints of mice with collagen-induced arthritis
Hoang Tu-Rapp,corresponding author1 André Hammermüller,1 Eilhard Mix,2 Hans-Jürgen Kreutzer,3 Roland Goerlich,4 Hansjürgen Köhler,1 Horst Nizze,3 Hans-Jürgen Thiesen,1 and Saleh M Ibrahim1
1Department of Immunology, University of Rostock, Rostock, Germany
2Department of Neurology, University of Rostock, Rostock, Germany
3Department of Pathology, University of Rostock, Rostock, Germany
4Institute of Biology VII, RWTH Aachen, Aachen, Germany
corresponding authorCorresponding author.
Hoang Tu-Rapp: hoang.tu-rapp/at/med.uni-rostock.de; André Hammermüller: andre.hammermueller/at/epost.de; Eilhard Mix: eilhard.mix/at/medizin.uni-rostock.de; Hans-Jürgen Kreutzer: hans-juergen.kreutzer/at/medizin.uni-rostock.de; Roland Goerlich: goerlich/at/molbiotech.rwth-aachen.de; Hansjürgen Köhler: hansjuergen.koehler/at/medizin.uni-rostock.de; Horst Nizze: horst.nizze/at/medizin.uni-rostock.de; Hans-Jürgen Thiesen: hans-juergen.thiesen/at/med.uni-rostock.de; Saleh M Ibrahim: saleh.ibrahim/at/med.uni-rostock.de
Received February 11, 2004; Revisions requested March 3, 2004; Revised April 30, 2004; Accepted June 7, 2004.
Abstract
Collagen-induced arthritis (CIA) is a chronic inflammatory disease bearing all the hallmarks of rheumatoid arthritis, e.g. polyarthritis, synovitis, and subsequent cartilage/bone erosions. One feature of the disease contributing to joint damage is synovial hyperplasia. The factors responsible for the hyperplasia are unknown; however, an imbalance between rates of cell proliferation and cell death (apoptosis) has been suggested. To evaluate the role of a major pathway of cell death – Fas (CD95)/FasL – in the pathogenesis of CIA, DBA/1J mice with a mutation of the Fas gene (lpr) were generated. The susceptibility of the mutant DBA-lpr/lpr mice to arthritis induced by collagen type II was evaluated. Contrary to expectations, the DBA-lpr/lpr mice developed significantly milder disease than the control littermates. The incidence of disease was also significantly lower in the lpr/lpr mice than in the controls (40% versus 81%; P < 0.05). However DBA-lpr/lpr mice mounted a robust immune response to collagen, and the expression of local proinflammatory cytokines such as, e.g., tumor necrosis factor α (TNF-α) and IL-6 were increased at the onset of disease. Since the contribution of synovial fibroblasts to inflammation and joint destruction is crucial, the potential activating effect of Fas on mouse fibroblast cell line NIH3T3 was investigated. On treatment with anti-Fas in vitro, the cell death of NIH3T3 fibroblasts was reduced and the expression of proinflammatory cytokines TNF-α and IL-6 was increased. These findings suggest that impairment of immune tolerance by increased T-cell reactivity does not lead to enhanced susceptibility to CIA and point to a role of Fas in joint destruction.
Keywords: apoptosis, Fas, rheumatoid arthritis, tolerance
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