Magnetic techniques for the isolation and purification of proteins and peptides

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Published online 2004 November 26. doi: 10.1186/1477-044X-2-7

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Table 9

Examples of peptides purified by magnetic techniques

Purified peptide	Source	Magnetic carrier	Affinity ligand	Further details	Reference
Biotinylated peptides	Model mixtures	Dynabeads M-280 streptavidin	Streptavidin	Used in MALDI-TOF mass analysis	[159]
(His)₆-Ala-Tyr-Gly	Synthetic peptide	Dynabeads M-280 tosylactivated	Aminocaproic nitrilotriacetic acid charged with Ni²⁺	Elution with imidazole solution	[160]
Synthetic pentapeptides against fructose-1,6-biphosphate aldolase	Synthetic mixture	Streptavidin-coated magnetic beads	Biotin labelled fructose-1,6-biphosphate aldolase of T. brucei	Pentapeptides were anchored on polystyrene beads	[161]
Tryptic digest products of cytochrome c	Trypsin digested cytochrome c	Au@magnetic particles	-	Ion-exchange separation followed by MALDI MS analysis	[31]
Glutathione		Gold and iron oxide nanocomposites			[162]
Nisin Z	Lactobacillus lactis	EDC activated magnetic beads	Anti-nisin antibody	Elution with 6 M urea	[163]

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