PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of biomagnBioMed Central web siteReference to the article.Search.Manuscript submission.RegistrationJournal front page.
 
From:
Published online Nov 26, 2004. doi: 10.1186/1477-044X-2-7
Table 8
Examples of other proteins purified by magnetic techniques
Purified proteinSourceMagnetic carrierAffinity ligandFurther detailsReference
AprotininBovine pancreatic powderMagnetic chitosan particlesTrypsinElution with low pH buffer[134]
Concanavalin AJack bean extractMagnetic particlesDextran[68,135]
Solanum tuberosum lectinPotato tuberMagnetic chitosanElution with low pH buffer[136]
Green fluorescent protein (histidine tagged)Magnetic nanoparticlesNi-NTAElution with imidazole containing buffer[137]
SIRT2 protein (recombinant, histidine tailed)HumanMagnetic agarose beadsNi-NTAElution with imidazole containing buffer[139]
Elongation factor (recombinant, histidine tailed)Caenorhabditis elegansMagnetic agarose beadsNi-NTAElution with imidazole containing buffer[140]
Protein ARecombinant Escherichia coliMagnetic EudragitHuman IgGMagnetic two-phase system[141]
Tumor necrosis factor (TNF)Dynabeads M-280Mouse monoclonal antibodySolid phase immunoassay[142]
Anti-MUC1 diabody fragmentRecombinant Escherichia coliMagnetic agarose beadsSpecific peptide[143]
MHC class II moleculesMDCK cellsDynabeads M-450 rat anti-mouse IgG1Specific antibodiesElution with SDS-PAGE buffer[144]
Lamin B3Xenopus egg extractsDynabeadsSpecific antibodiesElution with 6 M urea[44]
6x-His-tagged proteinsHuman fibroblastsMagnetic agarose beadsNi-NTAElution with imidazole containing buffer[145]
Estrogen receptorAdipose tissueDynabeads M-280 streptavidinBiotinylated monoclonal mouse anti-human estrogen receptor antibodyIndirect method[146]
Thiol-reactive chromatin restriction fragmentsMouse fibroblastsMercurated agarose magnetic beadsp-HydroxymercuribenzoateElution with 0.5 M NaCl and 20 mM dithiothreitol[147]
L1 coat proteinHuman papillomavirusMagnetic polyglutaraldehyde particlesIminodiacetic acid charged with Cu2+Elution with imidazole containing buffer[41]
Insulin receptorRat muscle or liver extractDynabeads M-450Anti-P5 antibodySDS PAGE analysis[148]
Stat3DER cellsDynabeadsBiotinylated tyrosine phosphorylated peptidesSDS PAGE analysis[149]
Transferrin receptorHumanDynabeads M-450 sheep anti-mouse IgG1Anti-human transferrin receptor monoclonal antibodySDS analysis[151]
Prion protein PrPScBrain extractDynabeads M-280 tosyl activatedPlasminogenSDS analysis[39,40]
Biotinylated proteins from extracellular matrixBipolaris sorokinianaDynabeadsStreptavidinSDS analysis[152]
CryoprotectinLeaves of cold-acclimated cabbage (Brassica oleracea)Dynabeads-protein ASpecific antibody[153]
Prostate specific antigenSerum from a prostate cancer suffering patientStreptavidin-coated magnetic beadsBiotinylated monoclonal antibodyElution with low pH solution[154,155]
Estrogen receptorIn vitro translationMagnetic beadsAntibodyElution with SDS buffer[156]
VHDL receptorHelicoverpa zeaStreptavidin-coated magnetic beadsVHDL-biotin ligand[157]
Fructosyllysine-specific binding proteinU937 cellsDynabeads M-280 tosylactivatedPoly-L-lysine-glucose conjugateTwo proteins isolated[158]
Ubiquitin (histidine tagged)Nickel-gold nanorodsElution with acidic buffer[18]
Articles from Biomagnetic Research and Technology are provided here courtesy of
BioMed Central