Magnetic techniques for the isolation and purification of proteins and peptides

doi:10.1186/1477-044X-2-7

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Biomagn Res Technol. 2004; 2: 7.

Published online 2004 November 26. doi: 10.1186/1477-044X-2-7

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Table 8

Examples of other proteins purified by magnetic techniques

Purified protein	Source	Magnetic carrier	Affinity ligand	Further details	Reference
Aprotinin	Bovine pancreatic powder	Magnetic chitosan particles	Trypsin	Elution with low pH buffer	[134]
Concanavalin A	Jack bean extract	Magnetic particles	Dextran		[68,135]
Solanum tuberosum lectin	Potato tuber	Magnetic chitosan		Elution with low pH buffer	[136]
Green fluorescent protein (histidine tagged)		Magnetic nanoparticles	Ni-NTA	Elution with imidazole containing buffer	[137]
SIRT2 protein (recombinant, histidine tailed)	Human	Magnetic agarose beads	Ni-NTA	Elution with imidazole containing buffer	[139]
Elongation factor (recombinant, histidine tailed)	Caenorhabditis elegans	Magnetic agarose beads	Ni-NTA	Elution with imidazole containing buffer	[140]
Protein A	Recombinant Escherichia coli	Magnetic Eudragit	Human IgG	Magnetic two-phase system	[141]
Tumor necrosis factor (TNF)		Dynabeads M-280	Mouse monoclonal antibody	Solid phase immunoassay	[142]
Anti-MUC1 diabody fragment	Recombinant Escherichia coli	Magnetic agarose beads	Specific peptide		[143]
MHC class II molecules	MDCK cells	Dynabeads M-450 rat anti-mouse IgG1	Specific antibodies	Elution with SDS-PAGE buffer	[144]
Lamin B₃	Xenopus egg extracts	Dynabeads	Specific antibodies	Elution with 6 M urea	[44]
6x-His-tagged proteins	Human fibroblasts	Magnetic agarose beads	Ni-NTA	Elution with imidazole containing buffer	[145]
Estrogen receptor	Adipose tissue	Dynabeads M-280 streptavidin	Biotinylated monoclonal mouse anti-human estrogen receptor antibody	Indirect method	[146]
Thiol-reactive chromatin restriction fragments	Mouse fibroblasts	Mercurated agarose magnetic beads	p-Hydroxymercuribenzoate	Elution with 0.5 M NaCl and 20 mM dithiothreitol	[147]
L1 coat protein	Human papillomavirus	Magnetic polyglutaraldehyde particles	Iminodiacetic acid charged with Cu²⁺	Elution with imidazole containing buffer	[41]
Insulin receptor	Rat muscle or liver extract	Dynabeads M-450	Anti-P5 antibody	SDS PAGE analysis	[148]
Stat3	DER cells	Dynabeads	Biotinylated tyrosine phosphorylated peptides	SDS PAGE analysis	[149]
Transferrin receptor	Human	Dynabeads M-450 sheep anti-mouse IgG1	Anti-human transferrin receptor monoclonal antibody	SDS analysis	[151]
Prion protein PrP^Sc	Brain extract	Dynabeads M-280 tosyl activated	Plasminogen	SDS analysis	[39,40]
Biotinylated proteins from extracellular matrix	Bipolaris sorokiniana	Dynabeads	Streptavidin	SDS analysis	[152]
Cryoprotectin	Leaves of cold-acclimated cabbage (Brassica oleracea)	Dynabeads-protein A	Specific antibody		[153]
Prostate specific antigen	Serum from a prostate cancer suffering patient	Streptavidin-coated magnetic beads	Biotinylated monoclonal antibody	Elution with low pH solution	[154,155]
Estrogen receptor	In vitro translation	Magnetic beads	Antibody	Elution with SDS buffer	[156]
VHDL receptor	Helicoverpa zea	Streptavidin-coated magnetic beads	VHDL-biotin ligand		[157]
Fructosyllysine-specific binding protein	U937 cells	Dynabeads M-280 tosylactivated	Poly-L-lysine-glucose conjugate	Two proteins isolated	[158]
Ubiquitin (histidine tagged)		Nickel-gold nanorods		Elution with acidic buffer	[18]

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