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Logo of biomagnBioMed Central web siteReference to the article.Search.Manuscript submission.RegistrationJournal front page.
 
From:
Published online 2004 November 26. doi: 10.1186/1477-044X-2-7

Table 7

Purification of albumin and haemoglobin by magnetic techniques

Purified proteinSourceMagnetic carrierAffinity ligandFurther detailsReference
Albumin, bovine serumCommercial preparationMagnetic agar beadsCibacron blue3GAAdsorption experiments[126]
Commercial preparationMagnetic cross-linked polyvinylalcoholCibacron blue3GAAdsorption experiments[76,127]
Magnetic chitosan microspheresCibacron blue3GA[78]
Commercial preparationMagnetic poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) particlesAnion exchange separation[128]
Commercial preparationMagnetic poly(ethylene glycol dimethacrylate-co-N-methacryloyl-(L)-histidine methyl ester) microbeadsCu2+Elution with 1.0 M NaSCN[129]
Albumin, human serumCommercial preparationMagnetic poly(2-hydroxyethylmethacrylate) beadsIminodiacetic acid charged with Cu2+Elution with 1.0 M NaSCN[130]
Human plasmaMagnetic poly(2-hydroxyethyl methacrylate) beadsCibacron blue F3GAElution with 0.5 M NaSCN[131]
Commercial preparationMagnetic particles covered with thermosensitive polymer-Desorption by decreasing temperature[132,133]
Albumin, human serum (recombinant, FLAG tagged)Yeast cellsMagnetic glass beadsAnti-FLAG antibodyElution with EDTA containing buffer[1]
Glycated haemoglobinHuman bloodMagnetic poly(vinyl alcohol) beadsm-Aminophenyl-boronic acidElution with sorbitol[138]
HaemoglobinBovine, commercial preparationAmine terminated iron oxide particlesIminodiacetic acid charged with Cu2+Elution with imidazole containing buffer[63]
Haemoglobin A1cHuman bloodMagnetic particles isolated from Magnetospirillum magneticum AMB-1m-Aminophenyl-boronic acidused for affinity immunoassay[150]