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Logo of biomagnBioMed Central web siteReference to the article.Search.Manuscript submission.RegistrationJournal front page.
Published online 2004 November 26. doi: 10.1186/1477-044X-2-7

Table 6

Examples of DNA/RNA/oligonucleotide/aptamer binding proteins purified by magnetic techniques

Purified proteinSourceMagnetic carrierAffinity ligandFurther detailsReference
CUG binding proteinsHuman myoblasts or fibroblastsDynabeads M-280 streptavidinBiotinylated(CUG)10Elution with 1 M NaCl[103]
Transcription factor τSaccharomyces cerevisiaeDynabeads M-280 streptavidinBiotinylated tRNAGlu gene fragmentElution with high salt buffer[104,105]
DNA-binding proteinsCrude tissue extractMagnetic phospho cellulose particles[106]
DNA-binding proteinsEscherichia coliMagnetic phospho cellulose particles[107]
DNA-binding proteinsHeLa nuclear extractsDynabeads M-280 streptavidinBiotin-labelled DNA fragmentElution with 2 M NaCl[108]
Vaccinia virus early transcription factorVaccinia virionsDynabeads M-280 streptavidinBiotinylated double-stranded DNAElution with high salt buffer[109]
Ecdysteroid receptorDrosophila melanogaster nuclear extractDynabeads M-280 streptavidinBiotinylated double-stranded oligonucleotideElution with 0.4 M KCl[110]
NanR protein (recombinant)Escherichia coliμMACS streptavidin MicroBeadsBiotin-labelled DNA fragmentElution with 1 M NaCl[111]
p27Rabbit hepatocytesDynabeads M-280 streptavidinGuanine-rich single-stranded DNAElution with NaCl solution[112]
Pigpen proteinEndothelial cellsMagnetic streptavidin beadsBiotinylated aptamerElution with 1 M NaCl[113]
RNA binding proteinsSaccharomyces cerevisiaeμMACS streptavidin MicroBeadsBiotin-labelled RNA probeElution with 1 M NaCl[114]
Single-stranded telomere binding protein (sTBP)Nuclei from vertebrate tissuesDynabeads M-280 streptavidinBiotinylated single stranded TTAGGGn repeatsElution with high salt buffer[115]
Transcription proteinsHuman myeloid cellsDynabeads M-280 streptavidinBiotinylated serum inducible element (hSIE)Elution with high salt buffer[116]
Transcription factor γRF-1Human monocytes and epidermal cellsDynabeads M-280 streptavidinBiotinylated DNA containing γRF-1 sequencesElution with 0.6 M KCl[117]
Protein factor MS2Murine skeletal myotubesDynabeadsDouble-stranded DNAElution with 100 mM sodium acetate, pH 4.2[118]
Guide RNA binding proteinTrypanosoma brucei mitochondriaDynabeads M-450 goat anti-mouse IgGMonoclonal antibodyElution with low pH buffer cont. SDS[119]
RNA binding proteinsPollen grainsStreptavidin MagneSphere particlesBiotinylated oligonucleotidesElution with SDS buffer[120]
DNA binding proteinSchistosoma mansoniDynabeads M-280 streptavidinBiotinylated DNAElution with sodium acetate buffer[121]
ssDNA binding proteinsTransfected mouse fibroblastsDynabeads anti-rabbit IgGRabbit antibodyIndirect method[122]
Tenascin-CGlioblastoma cellsDynabeads streptavidinBiotinylated aptamerElution with high salt buffer[123]
Thermostable brain factor (ThBF)Rat brainStreptavidin magnetic particlesBiotinylated oligonucleotidesElution with 0.7 M KCl[124]
TTF1 proteinEscherichia coli lysateDynabeads M-280 streptavidinBiotinylated aptamerElution with DNase[125]