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Published online 2004 November 26. doi: 10.1186/1477-044X-2-7
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Copyright © 2004 Safarik and Safarikova; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Table 5
Examples of antibodies purified by magnetic techniques
Purified antibodySourceMagnetic carrierAffinity ligandFurther detailsReference
Anti-BODIPY-fluorescein antibodiesMagnetoliposomesBODIPY-fluorescein[94]
Anti-DNA antibodySystemic lupus erythematosus patient plasmaMagnetic poly(2-hydroxyethyl-methacrylate) beadsDNADesorption with 1 M NaSCN solution[95]
Anti-human chorionic gonadotropin antibodyMurine ascites supernatantsMagnetic cellulose beadsHuman chorionic gonadotropin[96]
Antibody (from rat)Sample from affinity chromatographyDynabeads M-280Sheep anti-rabbit IgGAntibody concentration[3]
AntibodyRabbit serumDynabeads M-280Sheep anti-rabbit IgGElution with 0.5 M acetic acid[97]
Monoclonal antibodiesMouse hybridoma culture brothMagnetite particlesProtein A[98]
Anti-bovine serum albumin antibodiesThermosensitive magnetic microspheresBovine serum albuminImmobilization by the carbodiimide method[99]
Immunoglobulin G, humanCommercial preparationMagnetic poly(ethylene glycol dimethacrylate-N-methacryloly-L-histidine-methylester) beadsElution with 1 M NaCl[100]
Immunoglobulin GBlood serumCarboxyl-terminated magnetic particlesMproteinAG[101]
IgE antibodiesAllergic patients seraMagnetoliposomesAntigenic proteins[16]
Murine anti-fibroblast growth factor receptor 1 IgMAscitesPolystyrene magnetic beadsRat anti-mouse IgM monoclonal antibody[102]
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