PMCCPMCCPMCC

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Logo of biomagnBioMed Central web siteReference to the article.Search.Manuscript submission.RegistrationJournal front page.
 
From:
Published online 2004 November 26. doi: 10.1186/1477-044X-2-7

Table 3

Examples of polysaccharide and disaccharide hydrolases purified by magnetic techniques

Purified enzymeSourceMagnetic carrierAffinity ligandFurther detailsReference
α-AmylasesPorcine pancreas, Bacillus subtilis, wheat germMagnetic alginate beadsElution with 1 M maltose[4]
Bacillus amyloliquefaciens, porcine pancreasMagnetic alginate microbeadsElution with 1 M maltose[10]
β-AmylaseSweet potatoMagnetic alginate beadsElution with 1 M maltose[55]
β-GalactosidaseEscherichia coli homogenateSilanized magnetitep-Aminophenyl-β-D-thiogalactopyranosideElution with borate buffer, pH 10[58]
β-Galactosidase (fusion protein comprising the DNA-binding lac repressor)Bacterial lysateMagnetic beadsDNA containing Escherichia coli lac operatorElution with lactose analogue[64]
GlucoamylaseAspergillus nigerMagnetic alginate beadsElution with 1 M maltose[55]
PectinaseCommercial preparationMagnetic alginate beads[82]
PullulanaseBacillus acidopullulyticusMagnetic alginate beadsElution with 1 M maltose[55]