Magnetic techniques for the isolation and purification of proteins and peptides

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Published online 2004 November 26. doi: 10.1186/1477-044X-2-7

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Table 2

Purification of lysozyme by magnetic techniques

Purified enzyme	Source	Magnetic carrier	Affinity ligand	Further details	Reference
Lysozyme	Hen egg white	Magnetic chitin		Elution with 0.01 M HCl	[71]
	Hen egg white	Magnetic acetylated chitosan		Elution with 0.01 M HCl	[9]
	Commercial preparation	Magnetic poly(2-hydroxyethyl methacrylate)	Cibacron Blue F3GA	Elution with 1 M KSCN	[72]
		Magnetic chitosan beads		Magnetically stabilized fluidized bed	[73]
	Ornithodoros moubata	Magnetic chitin		Elution with alkaline, high salt buffer	[74]
	Commercial preparation	Magnetic cross-linked polyvinylalcohol	Cibacron blue 3GA	Elution with high salt buffer	[52]
		Magnetite – polyacrylic acid nanoparticles		Ion-exchange separation	[75]
		Magnetic cross-linked polyvinylalcohol beads		Adsorption study	[76]
	Commercial preparation	Magnetic agarose beads	Cibacron blue 3GA	Magnetically stabilized fluidized bed	[77]
		Magnetic chitosan	Cibacron blue 3GA	Study of adsorption properties	[78]
	Commercial preparation	Ferrofluid modified sawdust		Elution with 0.5 M NaCl	[79]
	Commercial preparation	Nano-sized magnetic particles		Elution with NaH₂PO₄and NaSCN	[80]
Lysozyme (recombinant, histidine-tailed)	T4	BioMag, amine terminated	Iminodiacetic acid charged with Cu²⁺	Elution with low pH buffer	[81]

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