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Logo of biomagnBioMed Central web siteReference to the article.Search.Manuscript submission.RegistrationJournal front page.
 
From:
Published online 2004 November 26. doi: 10.1186/1477-044X-2-7

Table 1

Examples of proteinases and peptidases purified by magnetic techniques

Purified enzymeSourceMagnetic carrierAffinity ligandFurther detailsReference
AminopeptidaseArabidopsisAmine-terminated magnetic beadsN-1-Naphthylphthalamic acidKCl gradient elution[54]
Angiotensin-converting enzymePig lung membranesDynabeadsPolyclonal antibodies[57]
BromelainCommercial preparationPolyacrylic acid – iron oxide magnetic nanoparticlesElution with KCl solution[59]
Caspase (recombinant, histidine-tailed)Human cellsMagnetic agaroseNi-NTAElution with SDS-PAGE buffer[60]
ChymotrypsinCommercial preparationMagnetic chitosan beadsElution with N-acetyl-D-tryptophan[62]
NIa-protease (recombinant, histidine-tagged)Plum Pox VirusMagnetic core and nickel-silica composite matrixNi2+Elution with imidazole containing buffer[36]
ProteinasesCommercial sourcesMagnetic cross-linked erythrocytesElution with low pH buffer[46]
Proteinase, bacterial (Savinase)Bacillus clausiiSilanized magnetite particlesBacitracin[84]
TrypsinPorcine pancreatinSilanized magnetite particlesp-AminobenzamidineElution with low pH buffer[50]
Porcine pancreatinMagnetic polymer particlesSoybean trypsin inhibitorElution with low pH solution[86]
Commercial preparationSilanized ferrite powderSoybean trypsin inhibitor[87]
Commercial preparationMagnetic κ-carrageenan particlesSoybean trypsin inhibitorElution with low pH solution, MSFB[88][89]
Commercial preparationMagnetic polyacrylamide beadsSoybean trypsin inhibitorMagnetically stabilized fluidized beds[90]
Commercial preparationMagnetic chitosan particlesAprotininElution with low pH solution[91]
Commercial preparationMagnetic cross-linked erythrocytesElution with low pH buffer; separation from large volume sample[19]
UrokinaseCrude urokinase preparationMagnetic dextran, agarose, polyvinyl alcohol, polyhydroxyethyl methacrylate microspheresp-Aminobenzamide, L-arginine methyl ester, guanidine hexanoic acid or guanidine acetic acid[93]