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Logo of scirepAboutEditorial BoardFor AuthorsScientific Reports
 
Sci Rep. 2017; 7: 46788.
Published online 2017 April 26. doi:  10.1038/srep46788
PMCID: PMC5405405

Corrigendum: Intronless WNT10B-short variant underlies new recurrent allele-specific rearrangement in acute myeloid leukaemia

Scientific Reports 6: Article number: 37201; published online: September 17 2016; updated: April 24 201710.1038/srep37201 [Cross Ref]

This Article contains typographical errors in the Methods section under subheading ‘WNT10B/WNT10BIVS1 Gene expression analysis’.

“The WNT10B (P4-P2 primers) amplification was performed with following thermal conditions: 94 °C for 1 min, 33 cycles at 94° for 30 s, 58 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min. The amplification of WNT10BIVS1 (P3-P2 primers) was performed as follows: 94 °C for 1 min, 33 cycles at 94° for 30 s, 61 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min”.

should read:

“The WNT10B (P4-P1 primers) amplification was performed with following thermal conditions: 94 °C for 1 min, 33 cycles at 94° for 30 s, 58 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min. The amplification of WNT10BIVS1 (P3-P1 primers) was performed as follows: 94 °C for 1 min, 33 cycles at 94° for 30 s, 61 °C for 30 s, 72 °C for 30 s and 72 °C for 5 min”.

In the same section, under subheading ‘WNT10B/WNT10BIVS1 Absolute quantification’,

“We performed the experiment on Bio-Rad’s QX100 ddPCR system and the reaction mixtures in a final 20 μl volume consisted of 10 μl of 2 × One-Step RT-ddPCR Supermix (Bio-Rad, CA, USA), 1 mM Manganese Acetate solution (Bio-Rad, CA, USA), 0.5 μM of primers (WNT10B: P4-P2, WNT10BIVS1 P3-P2), 0.25 μM WNT10B_dd1 and WNT10BIVS1_dd2 probes”.

should read:

“We performed the experiment on Bio-Rad’s QX100 ddPCR system and the reaction mixtures in a final 20 μl volume consisted of 10 μl of 2 × One-Step RT-ddPCR Supermix (Bio-Rad, CA, USA), 1 mM Manganese Acetate solution (Bio-Rad, CA, USA), 0.5 μM of primers (WNT10B: P4-P1, WNT10BIVS1 P3-P1), 0.25 μM WNT10B_dd1 and WNT10BIVS1_dd2 probes”.


Articles from Scientific Reports are provided here courtesy of Nature Publishing Group