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Bacteria produce a wide variety of exoproducts that favourably modify their environment and increase their fitness. These are often termed ‘public goods’ because they are costly for individuals to produce and can be exploited by non-producers (cheats). The outcome of conflict over public goods is dependent upon the prevailing environment and the phenotype of the individuals in competition. Many bacterial species use quorum sensing (QS) signalling molecules to regulate the production of public goods. QS, therefore, determines the cooperative phenotype of individuals, and influences conflict over public goods. In addition to their regulatory functions, many QS molecules have additional properties that directly modify the prevailing environment. This leads to the possibility that QS molecules could influence conflict over public goods indirectly through non-signalling effects, and the impact of this on social competition has not previously been explored. The Pseudomonas aeruginosa QS signal molecule PQS is a powerful chelator of iron which can cause an iron starvation response. Here, we show that PQS stimulates a concentration-dependent increase in the cooperative production of iron scavenging siderophores, resulting in an increase in the relative fitness of non-producing siderophore cheats. This is likely due to an increased cost of siderophore output by producing cells and a concurrent increase in the shared benefits, which accrue to both producers and cheats. Although PQS can be a beneficial signalling molecule for P. aeruginosa, our data suggest that it can also render a siderophore-producing population vulnerable to competition from cheating strains. More generally, our results indicate that the production of one social trait can indirectly affect the costs and benefits of another social trait.
Bacterial cells secrete numerous extracellular factors to favourably modify their environment. These include hydrolytic enzymes, protective polymeric matrices for biofilm formation and biosurfactants that aid motility. The benefits of such exoproducts can accrue both to the producing cell and to neighbouring cells and are therefore termed ‘public goods’ . Public goods are costly for individual cells to produce, and cooperating populations are consequently at risk of social exploitation by non-producing ‘cheats’ [1,2]. In theory, cheats can outcompete cooperators, because they do not incur the cost of public goods production, but derive benefits from the cooperation of others. Whether cooperation persists over evolutionary time in the face of the advantages of cheating is largely dependent on aspects of population structure that act to align individual interests .
Many cooperative behaviours seen in bacteria are regulated at the population level by cell-to-cell communication or quorum sensing (QS) systems [4–6]. Cells produce and release QS molecules to regulate the production of a range of public goods which aid in scavenging for nutrients, providing scaffolding for biofilms and facilitating motility. Because these cooperative secretions can be key determinants of successful growth or persistence, there has been considerable interest in the impact of QS on ecological competition between different genotypes or strains of bacteria [7–9]. For example, mutant genotypes which do not respond to QS molecules, and consequently produce fewer or no public goods (even though the loci that directly encode these public goods are intact), have been shown to act as social cheats both in vitro, in vivo and in biofilms [8,10–13]. In addition to regulating public goods production, QS molecules have been shown to have non-signalling effects, such as immune modulation, cytotoxicity, redox potential and iron binding [14,15]. The impact of these indirect effects by QS molecules on social competition has not previously been explored, and so here we empirically demonstrate how production of a QS molecule can alter the social landscape of a seemingly unrelated trait, siderophore production.
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen which employs a multi-layered QS system to regulate a number of public goods, many of which are important for virulence [4,5]. One well-defined P. aeruginosa QS signal is the pseudomonas quinolone signal (PQS) . PQS is a member of the 2-alkyl-4(1H)-quinolone family of molecules and acts as a QS molecule in the classical sense, in that it interacts with a specific receptor protein, and sets in motion a regulatory cascade leading to increased production of toxins and biofilms [16–18]. PQS also has other biological properties that are distinct from signalling: these include balancing redox reactions, aiding in competition with other species and interacting with cell membranes [17,19,20]. In addition, PQS has iron-chelating activity, though it does not act as a true siderophore, because it does not directly ferry iron into the cell [21,22]. It has therefore been suggested that PQS may act as an iron trap, aiding in the sequestration, but not in the membrane transport of iron .
Moving iron from either a host or the environment into the cell is often achieved by the production of dedicated iron scavenging molecules known as siderophores . Pseudomonas aeruginosa produces two major siderophores, pyoverdine and pyochelin. Pyoverdine has been experimentally demonstrated to be a public good , which is exploitable by cheats both in vitro and in vivo [25,26]. Here, we test whether the iron-chelating properties of PQS can change the social landscape of siderophore production. We show that PQS (i) increases the production of pyoverdine and pyochelin, and consequentially decreases the fitness of siderophore producers and (ii) increases the relative fitness of siderophore cheats in co-culture with a producing strain. Our findings highlight how direct modification of the environment by one bacterial exoproduct, in this case a QS signal molecule, can indirectly affect the evolutionary dynamics of another social trait.
For a rich, iron-replete growth environment, we used lysogeny broth (LB) (10 g l−1 tryptone, 5 g l−1 yeast extract, 10 g l−1 NaCL), and for an iron-limited growth environment we used casamino acids (CAA) medium (5 g l−1 CAA, 1.18 g l−1 K2HPO4.3H2O, 0.25 g l−1 MgSO4.7H2O). We prepared both media in dH2O and supplemented CAA medium with sodium bicarbonate solution to a total of 20 mM. For all experiments, we inoculated single colonies of the relevant bacterial strain into 5 ml LB and incubated at 37°C at 200 r.p.m. for 18 h. We then washed pre-cultures in the appropriate medium, corrected to an optical density of OD600 = 1.0, and inoculated experimental cultures to an initial density of OD600 = 0.01.
To study the effects on siderophores of varying concentrations of iron, PQS and its precursor 2-heptyl-4-hydroxyquinoline (HHQ), we used the strain PAO1ΔpqsAH, which is defective in 2-alkyl-4(1H)-quinolone production . To test whether investment in siderophores increased with added PQS or HHQ, we inoculated a washed pre-culture of PAO1ΔpqsAH into 750 µl LB medium containing varying concentrations of PQS and HHQ in microtitre plates and incubated at 37°C for 14 h. Following incubation, we measured the OD600 of resulting cultures, and then filtered the cell supernatants. We measured pyoverdine and pyochelin using excitation/emission assays (ex/em wavelengths 400 nm/460 nm for pyoverdine and 350 nm/430 nm for pyochelin  using a Tecan Multimode plate reader). We corrected fluorescence values by subtracting the fluorescence of a sterile medium blank and assuming a 5% leakage from the pyoverdine into the pyochelin channel, as previously described . We estimated per-cell siderophore production as fluorescence (relative fluorescence units) divided by culture density (OD600).
To measure the growth (fitness) of monocultures, we inoculated a washed pre-culture of either PAO1 or PAO1ΔpvdD/pchEF into 750 µl CAA medium supplemented with 20 mM NaHCO3 containing no addition, or supplementation with either 50 µM PQS, or 100 µg ml−1 (1.25 mM) transferrin in 48-well microtitre plates and incubated at 37°C for 14 h. To study the effect of PQS on the competition between siderophore producers and non-producers, we used wild-type PAO1 and a mutant that was defective in pyoverdine and pyochelin production and labelled with a constitutive luminescence marker (PAO1ΔpvdD/pchEF CTXlux). For competition assays, we pre-cultured, washed and density corrected both strains and mixed them to a ratio of approximately 99 : 1 (producer : non-producer). We incubated these in 5 ml iron-limited medium (CAA) in the presence and absence of 50 µM PQS for 24 h at 37°C with agitation at 200 r.p.m. To measure relative abundance of the strains, we plated the co-cultures before and after incubation, and counted total colonies and luminescent colonies. Relative fitness was calculated using the formula w = p1(1 − p0)/p0(1 − p1), where p0 and p1 are the proportion of non-producing mutants in the population before and after incubation, respectively .
The effect of PQS and HHQ supplementation on growth, and siderophore production were all analysed using the ordered heterogeneity approach . This allows for the evaluation of an ordered alternative hypothesis but does not require the fitting of curves. We chose this approach because our question is about the effect of increasing concentrations of PQS but without any concern for the exact shape of these relationships. For each test, we calculated the test statistic rs Pc = rS × (1 − p). p is the p-value from an ANOVA of raw data with concentration of PQS or HHQ fitted as a categorical variable, and rS is the absolute value of Spearman's rank correlation coefficient between the means of the relevant independent variable for each level of PQS or HHQ, and the concentration of PQS or HHQ. The relative fitness of a siderophore non-producer in iron-limiting conditions, and the effect of PQS supplementation on the relative fitness, were examined using t-tests. All statistical analyses were performed using R 3.0.2 .
To test whether PQS increased production of siderophores, we measured the amount of pyoverdine and pyochelin in cultures of a PQS-deficient mutant (PAO1ΔpqsAH) grown in LB, and supplemented with exogenous synthetic PQS at varying concentrations. We found that PQS reduced growth and increased the per-cell concentrations of pyoverdine and pyochelin in a concentration-dependent manner (figure 1a–c, ordered heterogeneity tests: growth F6,28 = 179, p < 0.001, rs = −0.964, rsPc = 0.964, p < 0.05; pyoverdine F6,28 = 1311, p < 0.001, rs = 0.643, rsPc = 0.643, p < 0.05; pyochelin F6,28 = 2222, p < 0.001, rs = 0.75, rsPc = 0.750, p < 0.05). As PQS plays a role in cell–cell communication, it is possible that reduced growth and induced iron scavenging are the result of QS-dependent regulation of gene expression. To exclude this possibility, we repeated the experiments in the presence of HHQ, the immediate precursor of PQS . HHQ does not bind iron, but maintains a signalling role in cell–cell communication [22,31]. We found that increasing concentrations of HHQ did not significantly affect growth, pyochelin or pyoverdine production (figure 1d–f; ordered heterogeneity tests: growth F6,28 = 9.1, p < 0.001, rs = 0.214, rsPc = 0.214, p > 0.05; pyoverdine F6,28 = 6.6, p < 0.001, rs = 0.25, rsPc = 0.250, p > 0.05; pyochelin F6,28 = 24.4, p < 0.001, rs = −0.071, rsPc = 0.071, p > 0.05). Overall, we conclude that it is the iron-chelating activity of PQS, and not its signal function, that triggers an iron starvation response: cells increase their production of iron scavenging siderophores and either a metabolic burden or slower uptake due to the presence of a chelator leads to poorer growth.
The production of siderophores is a social trait that can be exploited by non-producing cheats . We therefore predicted that intra-specific social competition over iron would intensify in the presence of exogenous PQS, due to the greater pool of siderophores available and the concomitant cost to producer growth. First we looked at the effect of PQS on monocultures of a PAO1 wild-type and a strain defective in the production of both pyoverdine and pyochelin (PAO1ΔpvdD/pchEF) in iron-limited media. The PAO1ΔpvdD/pchEF strain reached slightly higher optical densities than PAO1 in CAA media (p < 0.001), but we found that PQS reduced the fitness of both strains which is consistent with the iron chelation effects of PQS (figure 2a). We compared the PQS effect against the effect of transferrin, a chelator previously used in iron-limited media siderophore experiments [25,32]. We found similar reductions in growth which shows that the PQS iron-chelating effect is comparable with that of transferrin (figure 2a). Consistent with existing work on the social dynamics of siderophore production, we found that the PAO1ΔpvdD/pchEF mutant functioned as a ‘cheat’ in iron-limiting conditions, having a relative fitness greater than 1 when grown in co-culture with the wild-type (figure 2b; t1,10 = 3.32, p < 0.01), although the small increase in the fitness of the mutant (figure 2a) could partially explain this finding. In line with our hypothesis, the addition of PQS significantly increased the relative fitness of the mutant (figure 2b; F1,10 = 95.4, p < 0.001).
Here we show, for the first time, that environmental modification via a QS molecule affects the selection for public goods that are not, as far as we are aware, directly regulated by QS. Specifically, we show that the iron-chelating properties of PQS lead to increased production of costly siderophores and consequently, increased relative fitness of a siderophore cheat. We found that the addition of synthetic PQS to cultures of P. aeruginosa results in a concentration-dependent decrease in bacterial fitness (growth) and an increase in the production of the siderophores pyoverdine and pyochelin (figure 1). The biosynthetic precursor of PQS, HHQ (which does not bind iron), had only a small effect on the production of pyoverdine but no effect on the production of pyochelin or on growth (figure 1).
We hypothesized that this effect of PQS would enhance the relative fitness payoff of siderophore non-producing cheats in competition with the wild-type. Consistent with this hypothesis, we show that when siderophore production is increased by PQS in an iron-limited environment, this leads to an increase in the relative fitness of a cheating mutant (figure 2). The increased relative fitness of a cheat in the presence of PQS is likely due to a combination of the increased availability of siderophores to exploit, and the increased costs paid by siderophore-producing cells. Our findings complement and build upon previous work, which showed that when less iron is available to cells, this results in greater production of siderophores, and an increase in the relative fitness of cheats . In previous work, the authors artificially modified iron levels in the growth medium . Our work differs in that we show that direct modification of iron levels in the environment by a QS molecule can alter selection for siderophore production.
Overall, our work builds upon a growing body of experimental studies exploring the complexities of cooperation in P. aeruginosa, an organism that is an excellent laboratory model for applying and testing and extending social evolution theory [1,2,6,8,11,13,34,35]. Microbes produce a diverse array of public goods, and little is known about how social traits interact with each other either directly or indirectly , although recent work has shown an interconnection between pyoverdine and pyochelin production . Put another way, to what extent does the production of one social trait affect the social dynamics of another trait(s)? Existing examples include (i) the direct regulatory effect of communication on the production of public goods [7–9] and (ii) the genetic linkage of traits via pleiotropy . Future work in this area should continue to highlight and demonstrate which traits are social in microbes [38,39], but also begin to focus efforts on how apparently discrete traits interact, and how this affects population ecology and evolution within environments. This will require experiments that reveal the fitness effects of trait linkage, and also experiments to unravel the mechanisms by which traits are linked.
Given that we have shown PQS production enhances P. aeruginosa vulnerability to siderophore cheating, this suggests there are ecological and biological role(s) of PQS beyond its well-documented role as a QS signal [16–18]. 2-alkyl-4(1H)-quinolones (including HHQ) have previously been shown to be produced by several bacterial species, but to date, PQS has only been shown to be produced by P. aeruginosa [18,40], suggesting that PQS may have evolved functions distinct from signalling. One possibility previously suggested, is that PQS-bound iron associates with the bacterial envelope making it easier for dedicated siderophores to shuttle iron into the cell. This could ensure that metabolically expensive siderophores are not easily lost to other cells . Such a mechanism could help to reduce siderophore cheating, but our data show that siderophore cheats flourish when PQS is present. Another role could be in ‘privatizing’ iron for P. aeruginosa when it is in competition with other bacterial species. In this case, PQS-bound iron could reduce the availability of iron for heterospecific competitors while making it easier for pyoverdine and pyochelin to transport iron into cells. Future work focusing on understanding the ecology between bacterial species could help to unravel these interactions.
We thank Stuart West for comments on the manuscript.
R.P. and S.P.D. designed the experiments. R.P., F.H., A.C.S. and S.E. collected and analysed the data in the laboratory. R.P., F.H., L.M. and A.C.S. conducted the data analysis. R.P., L.M., P.W. and S.P.D. wrote the paper. All authors revised and approved the manuscript.
We have no competing interests.
This work was supported by the Natural Environment Research Council (grant number NE/J007064/1); the Human Frontier Science Program (grant number RGY0081/2012); the Wellcome Trust (grant number 095831); and the Biotechnology and Biological Sciences Research Council (grant number BB/J014508/1).