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The rapid spread of antibiotic-resistance among pathogenic bacteria poses a serious risk for public health. The search for novel therapeutic strategies and antimicrobial compounds is needed to ameliorate this menace. The bifunctional metalloenzyme CapF is an antibacterial target produced by certain pathogenic bacteria essential in the biosynthetic route of capsular polysaccharide, a mucous layer on the surface of bacterium that facilitates immune evasion and infection. We report the first inhibitor of CapF from Staphylococcus aureus, which was identified by employing fragment-based methodologies. The hit compound 3-isopropenyl-tropolone inhibits the first reaction catalyzed by CapF, disrupting the synthesis of a key precursor of capsular polysaccharide. Isothermal titration calorimetry demonstrates that 3-isopropenyl-tropolone binds tightly (KD=27±7μM) to the cupin domain of CapF. In addition, the crystal structure of the enzyme-inhibitor complex shows that the compound engages the essential Zn2+ ion necessary for the first reaction catalyzed by the enzyme, explaining its inhibitory effect. Moreover, the tropolone compound alters the coordination sphere of the metal, leading to the overall destabilization of the enzyme. We propose 3-isopropenyl-tropolone as a precursor to develop stronger inhibitors for this family of enzymes to impair the synthesis of capsular polysaccharide in Staphylococcus aureus.
Staphylococcus aureus (S. aureus) is a Gram-positive opportunistic bacterium causing life-threatening infections such as sepsis, endocarditis and pneumonia1,2. Alarmingly, the treatment against S. aureus is increasingly ineffective owing to the emergence of methicillin- and vancomycin-resistant strains3,4. The proliferation of antibiotic-resistant strains of S. aureus thus represents a therapeutic challenge and a menace for public health. For these reasons, efforts to find novel targets to overcome antibiotic resistance are in urgent need5.
The capsular polysaccharide (CP) is a polymeric carbohydrate attached to the outer surface of S. aureus critical for bacterial resistance because of its role in reducing the immune response of the host6,7. Targeting the synthesis of CP is therefore a potential therapeutic avenue to combat pathogenic strains of S. aureus. Most clinical isolates of S. aureus express either serotype 5 or serotype 8 CP composed of repeating units of L-FucNAc, D-FucNAc, and D-ManA. In particular, the biosynthetic pathway of the CP precursor UDP-L-FucNAc is well conserved among several pathogenic bacteria8. Targeting enzymes belonging to this pathway with inhibitors opens innovative strategies for the discovery of new therapeutic agents.
In S. aureus, the enzymes CapE, CapF and CapG catalyze the sequential transformation of UDP-D-GlcNAc in the CP precursor UDP-L-FucNAc via the intermediate compound UDP-N-acetyl-L-talosamine (UDP-L-TalNAc) (Fig. 1)9,10. Importantly, knockout and complementation studies have demonstrated the essential role of these enzymes for the synthesis of serotype 5 CP, justifying their potential as antibacterial targets8.
In previous studies we and others characterized the bifunctional enzyme CapF from structural, thermodynamic, and biochemical standpoints, laying the groundwork for the identification and characterization of inhibitors and drug-like compounds9,10,11,12. Structurally, CapF is a homo-dimer displaying a characteristic dumb-bell shaped architecture composed of two domains, each one catalyzing separate enzymatic reactions (Fig. 1)12. The C-terminal cupin domain of CapF catalyzes the epimerization of the compound produced by the upstream enzyme CapE, whereas the N-terminal SDR domain catalyzes the reduction of the compound afforded by the cupin domain, requiring one equivalent of NADPH. CapF is a metalloenzyme containing a Zn2+ ion in the cupin domain crucial for catalyzing the first chemical reaction, and also important for the stability of the enzyme12.
Here we have performed a screen with a small-molecule chemical library of fragments to identify compounds binding to and inhibiting the enzymatic activity of CapF. We describe the inhibition of the first reaction catalyzed by CapF by natural tropolones (7-membered ring aromatic compounds).Calorimetry and X-ray crystallography demonstrate that 3-isopropenyl-tropolone binds to the pocket of the cupin domain of CapF. The hit compound chelates the critical Zn2+ ion and exhibits specific non-covalent interactions with the enzyme as evidenced by the favorable binding enthalpy and its coordination chemistry. We discuss potential routes to increase the potency of this novel inhibitor of CapF.
To identify small-molecule inhibitors of CapF we employed fragment-based methodologies (Supplementary Information Figure S1)13,14. A fragment library consisting of 1,994 compounds was obtained from the Drug Discovery Initiative program (The University of Tokyo, see the Methods section for an brief description of the features of this library). This library was employed to screen for potential inhibitors of CapF using the technique of surface plasmon resonance (SPR). This screening methodology identified compounds with the ability to bind to the target protein but not by their inhibitory potency. Inhibition was only assessed with the selected hit compound using two separate enzymatic assays (see below). To maximize the sensitivity of the SPR signal the microtiter-based assay mode was employed. The values of the parameters CV (1.0%) and Z-factor (0.93) were monitored throughout the screen15. Fragments displaying box-type kinetic responses within the top 10% binding responses were considered for further characterization (Supplementary Information Figure S1)16,17.
A total of 15 compounds selected from the library screening were next evaluated with SPR at three different concentrations (100μM, 10μM, and 1μM) to detect compounds giving false positive signals, which are generally known as nonspecific and promiscuous binders. Five fragments exhibiting binding responses in a concentration-dependent manner were selected for additional evaluation. Lastly, we determined the binding affinity of these five compounds for CapF with SPR. Only 3-isopropenyl-tropolone bound to CapF with sub-millimolar affinity, a threshold we judged necessary to justify further analysis (Fig. 2a, and Supplementary Information Figure S1e). The other four compounds displayed low affinities (mM range) and were not considered for additional examination.
Although 3-isopropenyl-tropolone binds robustly to CapF, as shown above, it was necessary to verify whether it also inhibits the enzymatic activity of the protein or not. For that purpose, an HPLC-based assay monitoring the consumption of substrate and the appearance of product was employed (Fig. 2b)12. Whereas in the absence of 3-isopropenyl-tropolone the substrate was converted into product with nearly quantitative yield (91%), in the presence of 1mM 3-isopropenyl-tropolone the peaks corresponding to substrate and product were largely absent (their combined areas were <18%). Instead, a large peak corresponding to the byproduct generated by the enzyme CapE under thermodynamic control was observed (80% yield as judged from the area under the curve). To confirm that inhibition of 3-isopropenyl-tropolone is specific for CapF we performed an additional experiment employing only CapE, which is the enzyme generating the substrate for CapF in the functional assay (Supplementary Information Figure S2). The HPLC profile of the reaction products was not altered in the presence of inhibitor, ruling out the possibility that the hit compound also inhibits CapE.
A second assay monitoring the consumption of NADPH corroborates the inhibitory effect of 3-isopropenyl-tropolone (Fig. 2c). This assay is sensitive to the NADPH consumed by the SDR domain during the second reaction catalyzed by CapF (reduction step). It was observed that the rate of consumption of NADPH slowed down as the concentration of inhibitor increases, indicating direct inhibition of CapF. The accumulation of the byproduct generated by CapE in the presence of inhibitor (Fig. 2b) suggested that inhibition occurs in the cupin domain of CapF. Nevertheless, additional experiments were required to corroborate that idea (see below).
Because tropolone and its analogs have been described as metal chelators in proteins18, we anticipated that binding of the inhibitor to CapF is governed by the Zn2+ ion present in the cupin domain12. To confirm this hypothesis, we performed two different types of experiments. First, we determined the binding properties of 3-isopropenyl-tropolone to apo-CapF (i.e. Zn2+-depleted CapF) and to holo-CapF (reconstituted with Zn2+) by isothermal titration calorimetry (ITC). When holo-CapF was employed, the compound bound robustly as demonstrated by the exothermic sigmoid isotherm shown in Fig. 3a. The stoichiometry is consistent with a 1:1 binding model. The dissociation constant (KD) determined by non-linear regression was 27±12μM. In contrast, 3-isopropenyl-tropolone does not bind to CapF when apo-CapF (Zn2+-depleted) was employed (Fig. 3b). We note that binding of 3-isopropenyl-tropolone to free-Zn2+ in solution was not detected by ITC under our experimental conditions (Supplementary Information Figure S3a), suggesting that specific interactions between Zn2+ ion and the inhibitor occur only in the context of the binding pocket of the enzyme.
Second, the fluorescence of the Zn2+-specific indicator FluoZin-319,20 was examined in the presence of CapF. The signal of FluoZin-3 increases dramatically in the presence of Zn2+ ions when the metal is accessible to the dye. Hence, the high fluorescence signal of the indicator in the presence of holo-CapF indicated that Zn2+ is exposed in the pocket of the cupin domain for interaction with the fluorescent dye (Fig. 3c, and Supplementary Information Figure S3b). This result is consistent with the location of Zn2+ in a region accessible to the solvent, as previously reported in the crystal structure of CapF12. In contrast, the fluorescence of FluoZin-3 with apo-CapF, or with holo-CapF in the presence of 3-isopropenyl-tropolone, was negligible. We also observed that, in the presence of holo-CapF, the fluorescence signal of FluoZin-3 depends on the concentration of hit compound in solution, exhibiting a sigmoid shape with an inflexion point at 51μM (Fig. 3c).
A control experiment with CapF lacking the His6-tag was carried to rule out the possibility of spurious effects caused by the affinity tag (Supplementary Information Figure S4). The titration profile and thermodynamic parameters corresponding to the binding of 3-isopropyl-tropolone to CapF lacking the His6-tag are very similar to those observed with the tagged enzyme, thus demonstrating the affinity-tag bears negligible influence for the interaction between enzyme and inhibitor. Collectively, the data above clearly suggest that the key Zn2+ ion in the cupin domain is essential for the binding of 3-isopropenyl-tropolone to the enzyme.
To examine the structural basis for the binding of 3-isopropenyl-tropolone to CapF we employed X-ray crystallography. A construct lacking the loop 57-70 at the N-terminal SDR domain (CapFΔ57-70) was employed for the structural characterization of the enzyme-inhibitor complex. Although this loop does not interact with the cupin domain in the structure of the dimer, it establishes intermolecular interactions with another dimer of CapF in the crystal form (PDB entry codes 3ST7 and 3VHR)12. In these two independent crystal structures of WT CapF, it is observed that residues belonging to the loop 57-70 of one dimer of CapF insert in the Zn2+ pocket of the cupin domain of a different dimer, presumably stabilizing the crystal lattice (Supplementary Information Figure S5). This crystallographic artifact may interfere with binding of 3-isopropenyl-tropolone to the Zn2+-pocket in soaking experiments with crystals of WT-CapF by blocking access of the compound. To avoid this severe complication, we employed the truncated construct CapFΔ57-70 and successfully obtained the structure of the enzyme-inhibitor complex. These intermolecular interactions are negligible in solution, since CapF is dimer, and not a tetramer or higher assembly12. We also note that the truncation does not modify the thermal stability of the resulting enzyme with respect to WT CapF as judged from the values of the mid-point transition (TM) obtained by differential scanning calorimetry (DSC) (ΔTM<1°C, Table 1).
The crystal structure of the complex of CapF with inhibitor was determined at 2.4Å resolution (Fig. 4, Table 2). The asymmetric unit contained a dimer of CapF bound to two molecules of inhibitor (i.e. the stoichiometry was 1:1). The value of rmsd between the coordinates of unliganded WT CapF (PDB entry code 3ST7)12 and CapFΔ57-70 in complex with 3-isopropenyl-tropolone is small (all-atoms rmds=0.7Å) demonstrating the enzyme does not undergo major conformational changes upon ligand binding. The molecule of 3-isopropenyl-tropolone is bound deep inside the active site of the cupin domain of CapF near the Zn2+ ion (Fig. 4a). The carbonyl and hydroxyl groups of the tropolone ring are found within the coordination sphere of Zn2+ at distances of 2.2Å and 1.9Å, respectively. The compound buries 230±1Å2 in the binding pocket of the enzyme, i.e. ~75% of the compound’s total accessible surface area21. The seven-membered ring of 3-isopropenyl-tropolone makes extensive van der Waals contacts with residues Phe262, Lys285, Gly286, His288, Phe297, Ile339 and Met351 of the cupin domain (Fig. 4b,c). No contacts with residues of the SDR domain are observed. The value of the shape complementarity factor (Sc) between th inhibitor and CapF was 0.74±0.01, indicating greater complementarity than that between a typical antibody-antigen complex (0.64–0.68)22.
Superposition of the coordinates of bound and unbound CapF revealed a shift in the position of Zn2+ by approximately 1.4Å towards the tropolone ligand, resulting in the disruption of the coordination bond between Zn2+ and the residue His290 (Fig. 4d). The disruption of the coordination sphere of Zn2+ by the inhibitor results in a significant decrease in the thermal stability of CapF in comparison with the apo protein (ΔTM=5.2±0.4°C for WT CapF and ΔTM=6.3±0.2°C for CapFΔ57–70) (Table 1, Supplementary Information Figure S6). Taken together, the interaction between 3-isopropenyl-tropolone and CapF seems specific because of the combination of strong coordination bonds to the metal and the high shape complementarity with residues of the binding pocket of the cupin domain.
We examined several analogs of 3-isopropenyl-tropolone, namely tropolone, 4-isopropyl-tropolone, tropone, and 2-Cl-tropone, to rationalize the basis of their interaction with CapF (Supplementary Information Figure S7). To evaluate the relative inhibitory potency of the compounds we measured the relative consumption of NADPH. The results indicate that the three derivatives of tropolone (3-isopropylen-tropolone, tropolone, and 4-isopropyl-tropolone) inhibited the consumption of NADPH in a concentration-dependent basis (Fig. 5, Supplementary Information Figure S7). In contrast, the two analogs of tropone (tropone and 2-Cl-tropone) lacking the crucial hydroxyl group chelating the Zn2+ ion did not significantly inhibit the enzymatic activity of CapF.
ITC was employed to investigate the dissociation constant and thermodynamic signature of binding. Consistent with the inhibition experiments from above, binding of tropone and 2-Cl-tropone to CapF was not detected, whereas 3-isopropylen-tropolone and tropolone bound to CapF with similar affinity (27±12 and 38±7μM, respectively) and comparable thermodynamic signatures (Fig. 3a, Table 3, and Supplementary Information Figure S8). In particular, the thermodynamic data indicated that the binding of these two tropolone compounds is driven by the enthalpy term (ΔH<0), suggesting favorable non-covalent interactions between the compounds and the protein. We note that the change of entropy was also favorable, and of similar magnitude to the value of ΔH in these two tropolone compounds (Table 3). In contrast, the dissociation constant of 4-isopropyl-tropolone was substantially larger (KD=1.8mM) indicating weaker affinity and resulting in very little exothermicity. Because of the low exothermic heat recorded during the titration with 4-isopropyl-tropolone, the value of enthalpy was not reliable and hence is not reported. Overall, these data demonstrate the essential role of the hydroxyl group in position C2 of the ring for metal chelation, and the unfavorable effect of enlarging the tropolone moiety at position C4 of the ring, but not at position C3, for the binding affinity.
The data presented in this work have revealed that natural tropolones are robust inhibitors of CapF, and well-suited for optimization to increase their potency as we explain below. First, these compounds are derivatives of the natural compound tropone, belonging to a family of compounds with diverse biological activities23. A recent report has suggested that tropolones are potent inhibitors of various metalloenzymes showing high ligand efficiencies (LE=−ΔG/HAC, where ΔG is the binding free energy, and HAC is the number of heavy atoms)24. In particular, the high value of LE observed for 3-isopropenyl-tropolone (LE=0.52kcal mol−1) and tropolone (LE=0.67kcalmol−1) found with CapF suggest that this family of compounds has the potential of being potent inhibitors of CapF24. Second, based on the structure-activity relationship analysis carried out and the crystallographic data obtained, these compounds seem amenable to optimization (to increase affinity and specificity) using rational structural-based ligand design methodologies, such as the target-based drug discovery and the lead optimization methodologies for the design of antibiotic agents25,26. And third, the thermodynamic data demonstrate that the binding of tropolones to CapF is driven by favorable changes of enthalpy. It is generally considered that among compounds of fragment-size, exothermic binding is an important advantage for ligand optimization because enthalpic binding accomplishes highly oriented interactions making these candidates less promiscuous binders27,28.
In summary, we performed a fragment-based screening to find suitable inhibitors of the antibacterial target CapF, a bifunctional enzyme of the biosynthetic route of CP of S. aureus. We identified the hit compound 3-isopropenyl-tropolone inhibiting the first reaction of CapF. Calorimetry and X-ray crystallography demonstrates that 3-isopropenyl-tropolone binds to CapF exothermically, forming coordination bonds with the crucial Zn2+ ion and extensive non-covalent interactions with residues of the active site of the cupin domain. We expect that the extension of the ring at the C3-position of the tropolone ring will yield compounds of increasing potency. We propose tropolones as plausible candidates to inhibit the synthesis of the capsular polysaccharide of pathogenic strains of Staphylococcus aureus.
The fragment library employed in this study was provided by the Drug Discovery Initiative (The University of Tokyo, Japan). This library, comprising 1,994 compounds, was designed to contain commercially available compounds excluding those with potential reactive chemical structures, but securing diversity from chemical descriptors. The library is compliant with the “rule of three”. Compounds are soluble to at least 200μM in standard buffers at neutral pH when supplemented with 2% DMSO. The analogs of tropone and tropolone were purchased from commercial suppliers.
CapF was expressed and purified as described previously12. Briefly, CapF with a His6-tag at the N-terminal was expressed in Escherichia coli Rosetta2 (DE3) cells. Cells were harvested and disrupted by sonication in lysis buffer containing 50mM TRIS-HCl (pH 8.0) and 500mM NaCl. The cell lysate was centrifuged at 40,000g for 30min at 4°C, and the supernatant loaded on a HisTrap column (GE Healthcare) equilibrated with lysis buffer. Protein was eluted with buffer supplemented with 0.5M imidazole. Protein fractions containing CapF were pooled together, concentrated and loaded on a HiLoad 26/60 Superdex 200 gel-filtration chromatography column (GE Healthcare) equilibrated with lysis buffer. Fractions of CapF were concentrated and stored as above. CapF without additional treatment was employed for the screening step, NADPH consumption assay, binding concentration dependence assay by surface plasmon resonance (SPR), and inhibition assay by HPLC. When metal-depleted CapF was required, the purified enzyme was dialyzed with HEPES buffered-saline (20mM HEPES, 150mM NaCl, pH 7.4, abbreviated HBS) containing 1mM EDTA, followed by a second dialysis with HBS. To prepare CapF fully occupied with Zn2+, the enzyme was dialyzed with HBS supplemented with 1μM ZnCl2, followed by a dialysis in HBS without Zn2+. CapF was subjected to size exclusion chromatography to remove aggregated material after the second dialysis with HBS. The Zn2+-reconstituted CapF was employed in calorimetric and NADPH consumption assays. Purification of the enzyme CapE (required for the enzymatic assays) was carried out as described earlier12.
Fragment screening was conducted in a Biacore T200 instrument (GE Healthcare). CapF was immobilized on a CM5 sensor chip (GE Healthcare) by the amine-coupling method29. Sensorgrams were obtained by injecting the compounds into a sensor chip decorated with CapF in a buffer composed of 10mM HEPES, pH 7.4, 150mM NaCl, 0.005% (v/v) Tween-20, 5% (v/v) DMSO, and 1mM DTT at a flow rate of 30mL min−1. Positive and negative control assays were performed periodically to confirm the stability of the protein on the sensor chip by injecting NADPH (100μM) and buffer, respectively. NADPH was chosen for the control assays because it exhibits a suitable affinity for CapF (KD=1.6μM)12. Measurements were carried out at 25°C.
The enzymatic performance of CapF was evaluated by examining the consumption of NADPH spectrophotometrically, or by monitoring the disappearance of substrate and the formation of product by HPLC9,12. First, the time-dependent consumption of NADPH (monitored from a decrease of adsorption at 340nm) was carried out in 384-well plates at a final volume of 50μL. The solution contained 1.0μM CapE, 0.02μM CapF, 125μM UDP-D-GlcNAc (substrate), 250μM NADPH and fragment compounds at various concentrations in HBS supplemented with 0.005% (v/v) Tween-20 and 5% (v/v) DMSO. Each reaction mixture was incubated at 37°C and the absorption at 340nm was measured with a plate reader (PHERAstar, BMG Labtech, Durham, NC).
For the HPLC-based inhibition assay, a solution containing 1.0μM CapE, 0.02μM CapF, 250μM UDP-D-GlcNAc, 250μM NADPH, and 1mM compound in HBS buffer supplemented with 0.005% (v/v) Tween-20 and 5% (v/v) DMSO were incubated at 37°C for 2hours. The reaction was stopped by addition of 100μL of ice-cold phenol/chloroform/isoamyl alcohol in a 25:24:1 molar ratio. The supernatant containing the products of the reaction was mixed with 100μL of chloroform and examined by HPLC using a CarboPac PA1 anion-exchange column (Dionex) as described previously9,12. Identification of monosaccharides in the elution profile was based on a previous report9.
Binding constants and thermodynamic parameters of the interaction between CapF and fragment compounds were determined with an iTC200 instrument (GE Healthcare) at 25°C. Samples were equilibrated in a buffer composed of 10mM HEPES (pH 7.4) and 150mM NaCl supplemented with 5% (v/v) DMSO. CapF at 150–270μM was titrated with compounds at 0.5–1.65mM. Analysis of the data was performed with ORIGIN7 using a one-site binding model.
Thermal stability of CapF was examined by DSC in a ultrasensitive VP-capillary micro-calorimeter (Microcal). Prior to the experiment the protein was equilibrated in a buffer composed of 50mM HEPES (pH 7.5), 50mM NaCl and 5% DMSO. The concentration of protein and inhibitor were adjusted to 24μM and 240μM, respectively. The samples were heated between 10 and 110°C at a scan rate of 1°C/min. Data analysis was carried out with ORIGIN7 using a two-state transition model.
Fluorescence measurements were carried out using the Zn2+-sensitive dye FluoZin-3 (Life Technologies) in a buffer composed of 10mM HEPES, 150mM NaCl (pH 7.4) supplemented with 5% (v/v) DMSO at 25°C. The fluorescence intensities were measured in a plate reader (PHERAstar, BMG Labtech, Durham, NC) with excitation and emission wavelengths set to 490nm and 520nm, respectively.
The sequence of CapFΔ57–70 was inserted in a modified pET26 vector with a His6-SUMO-GGS tag at the N-terminus. The protein was expressed in Escherichia coli BL21(DE3) cells and induced with 0.5mM isopropyl β-D-1-thiogalactopyranoside at 20°C for 12hours. Cells were harvested, resuspended in lysis buffer supplemented with 20mM imidazole, sonicated, and the cell debris discarded after a centrifugation step at 40,000×g for 30min at 4°C. The supernatant was applied onto a Ni-NTA agarose column (Qiagen) and the protein eluted with a solution containing 300mM imidazole. The protein fractions were treated with the protease Ulp1 at 1% (w:w) and simultaneously dialyzed in a buffer containing 20mM Tris-HCl, 50mM NaCl, 1mM DTT, and 10μM ZnCl2 at 4°C overnight. After dialysis the samples were passed again through a Ni-NTA column. The flow-through sample was concentrated and subjected to size exclusion chromatography in a HiLoad 26/60 Superdex 200pg column (GE Healthcare) in a buffer composed of 50mM HEPES and 50mM NaCl (pH 7.5). The fractions containing truncated CapF were concentrated to 10mg ml−1 and used for crystallization trials. After one round of screening using an Orix8 instrument (Douglas Instruments), single crystals were obtained by mixing 2–3μL of protein with 2–3μL of reservoir solution containing 200mM KH2PO4, 10% PEG 3,350, and sodium cacodylate (pH 7.2). Suitable crystals of CapF was transfer to a new drop containing 1.8mM 3-isopropyl-tropolone dissolved in 5% DMSO for three hours, after which they were briefly transferred to the same solution supplemented with 20% glycerol, and stored in a vessel containing liquid N2 until data collection.
Diffraction data were collected at beamline BL5A of the Photon Factory (Tsukuba, Japan) under cryogenic conditions (100K). Diffraction images were processed with the program MOSFLM and merged and scaled with the program SCALA of the CCP4 suite30. The three dimensional structure of truncated CapF was determined by the method of molecular replacement using the coordinates of the full-length protein (PDB entry code 3ST7)12 with PHASER31. The initial model was refined with REFMAC532 and COOT33. The coordinates of 3-isopropenul-tropolone were generated with PRODRG34. Validation was carried out with PROCHECK35.
How to cite this article: Nakano, K. et al. Discovery and characterization of natural tropolones as inhibitors of the antibacterial target CapF from Staphylococcus aureus. Sci. Rep. 5, 15337; doi: 10.1038/srep15337 (2015).
The authors thank Ms. Chizuru Saruta for collecting fluorescence and ITC data. This work was supported by Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by a Grant-in-Aid for Scientific Research A (Grant Number 25249115) from the Japan Society for the Promotion of Science. Access to beamline PF-BL5A was granted by the Photon Factory Advisory Committee (Proposal No. 2014G190).
The authors declare no competing financial interests.
Author Contributions T.M., S.N. and T.K. designed the overall study. K.N., T.C., T.M., S.N. and J.M.M.C. performed experiments. All authors designed research and analyzed the data. J.M.M.C., S.N. and T.M. wrote the manuscript with input from the other authors.