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Vet World. 2017 March; 10(3): 311–318.
Published online 2017 March 13. doi:  10.14202/vetworld.2017.311-318
PMCID: PMC5387658

Can methicillin-resistant Staphylococcus aureus prevalence from dairy cows in India act as potential risk for community-associated infections?: A review

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is classified as hospital associated (HA), community associated (CA), livestock associated (LA) and is a global concern. Developing countries, like India, are densely populated country challenging for public hygiene practices. HA-MRSA is comfortably recorded in India, and CA-MRSA is also reported as increasing one. CA-MRSA is serious disease which affects the community as endemic. MRSA is one among major mastitis-causing organisms in India as LA-MRSA. There were reports for transmission of MRSA as community between milk handlers and cow in global perspective. In India reports of MRSA in short among milk handlers and also transmission between animal and human. Hence, proper monitoring of MRSA transmission in India should be elucidated in account among milk handlers and dairy cows to avoid emerging CA-MRSA as outbreak.

Keywords: community associated, dairy cow, livestock associated, methicillin-resistant Staphylococcus aureus, methicillin-resistant Staphylococcus aureus transmission, milk handlers, monitoring

Introduction

Staphylococcus aureus is the well-known epidemic nosocomial pathogen in humans [1,2] and also the primary causative agent of mastitis in cattle [3,4]. S. aureus is considered as a significant pathogen with related virulence factors such as slime factor (biofilms), panton-valentine leukocidin (PVL), and some enzymes (proteases, lipases, and elastase), which facilitates destruction of host tissues and metastase to other sites [5], treatment of S. aureus infections included semisynthetic penicillin drugs, such as methicillin [6]. However, in the 1960’s, the rise of methicillin-resistant S. aureus (MRSA) strains was apparent [7].

MRSA is primarily mediated by the mecA gene carried on a mobile genetic element (MGE), the staphylococcal cassette chromosome mec (SCCmec), and at least five types of SCCmec elements have been reported SCCmec type I, II, III, IV, V, VI [8]. To date, SCC elements have been identified within Staphylococcus sciuri [9], Staphylococcus hominis [10], Staphylococcus epidermidis [11], Staphylococcus haemolyticus [12], and S. aureus [13]. mecA gene codes for the modified penicillin-binding protein 2a (PBP 2a or PBP 2’). PBP2a is positioned in the bacterial cell wall and has a low binding affinity for β-lactams. The role of inappropriate antibiotic usage, under dosage, and inappropriate administration are also considerable in acquiring antibiotic resistance. MRSA has become apparent as a major cause of hospital-associated (HA) and community-associated (CA) infections [14] and also isolated from milk (livestock associated [LA]) [15].

HA-MRSA versus CA-MRSA

HA-MRSA (HA-MRSA) characteristically colonizes or infects hospitalized individuals with predisposing risk factors, usually retain SCCmec type I, II or III, and is multi-drug resistant (MDR) [16]. Whereas, CA-MRSA infects healthy individuals without any previous health-care contact, often retains smaller and more mobile SCCmec types, is usually PVL positive, susceptible to non-β-lactam antimicrobial drugs, and frequently evident as skin and soft-tissue infections. However, this difference between CA- and HA-MRSA is gradually dwindling owing to the emergence of pvl negative and/or MDR CA-MRSA clones, and its invasion into hospitals. The incidence of HA- and CA-MRSA infections, as well as the relative abundance of different MRSA clones, varies substantially among countries. The HA-MRSA is endemic in many hospitals worldwide [17]. The CA-MRSA has a smaller fitness cost, higher transmissibility and virulence compared to HA-MRSA, and is epidemic in many geographical locations [5]. Limited options are available for the therapeutic management of MRSA infections. The CA-MRSA-associated skin and soft-tissue infections are treated with oral antibiotics including minocycline, doxycycline, clindamycin, rifampicin, sulfamethoxazole, trimethoprim, and fusidic acid. Severe CA-MRSA infections and HA-MRSA requisites intravenous vancomycin therapy. Asymptomatic carriers represent an important MRSA reservoir [18]. In India, INSAR study report [19] showed that the MRSA in 26310 S. aureus isolates during the study phase between January 2008 and December 2009 in 15 tertiary centers was 41%. The antibiotic resistant pattern of MRSA isolates in above study indicated that lower susceptibility to ciprofloxacin, gentamicin, cotrimoxazole, erythromycin, and clindamycin, but no isolates found resistant to vancomycin or linezolid.

Development of CA-MRSA

In recent years, there have been several reports of CA-MRSA infections worldwide; including several outbreaks in the United States [20-22]. Most of these outbreaks have been associated with a single-clone strain. The transmission has occurred by close physical contact in conditions involving children in day-care centers, children, adults, athletes, army personnel, correctional facilities, and homosex [23-25]. Of concern, these patients are otherwise healthy individuals with no known risk factors for MRSA acquisition [26]. In India, the incidence of MRSA shows a large variation, from 6.9% to 81% [27]. As with Gram-negative bacteria in the Indian subcontinent, the widespread use of antibiotics, poor public health infrastructure, and a congested population will probably lead to the emergence and dissemination of antibiotic-resistant lines of S. aureus. Additional factors favoring the spread of S aureus are high rates of skin infections, such as scabies and impetigo. Recent reports suggest that CA-MRSA is increasing widespread in India with findings from a single-center study in 2011-2012 showing that 65% of CA S. aureus infections were due to MRSA, and findings from another study manifests that more than 70% of healthy carriers of S. aureus carried MRSA [28]. D’ Souza et al. [29] studied the cases of MRSA and found that 54% were true CA-MRSA possessing the SCCmec IV and SCCmec V genes. These were mainly separated from SSTIs. CA-MRSA isolates also showed variable resistance to ciprofloxacin, erythromycin, clindamycin, and tetracycline. In Brazil, a single nosocomial MRSA clone, the so-called pandemic Brazilian MRSA clone (sequence type [ST]239, SCCmec III), has been responsible for the overwhelming majority of nosocomial infections for the last 20 years [30,31]. Rates of MRSA are up to 60% and are related to an endemic Brazilian clone. A vancomycin-resistant MRSA is also reported in hospital and CA infections of Brazil. Chatterjee et al. [32] found the overall prevalence of S. aureus nasal colonization was 52.3% and that of MRSA was 3.89% in the community. In major southern districts of Tamil Nadu, 31.1% of staphylococcus strains were found to be methicillin resistant [33].

Importance of CA-MRSA

Outbreaks of CA-MRSA were first described in the early 1980s and in the 1990s increasing reports began to emerge. CA-MRSA is now a common community-based pathogen demonstrated great geographic diversity with outbreaks reported in the United states, Canada, Europe, Finland, Saudi Arabia, India, Australia, and New Zealand [34]. The emergence and global dissemination of MDR Gram-negative bacteria from India has received much attention. Less attention, however, has been given to records describing the emergence in the last 5 years of two CA-MRSA lines from the India–ST772 and ST22. Both lineages express PVL, which is related to skin and soft-tissue infections. ST772 and ST22 MRSA expressing PVL have become increasingly common in India and have caused frequent outbreaks and infections elsewhere in the world, which is often epidemiologically linked to India [35-37].

Livestock Associated MRSA (LA-MRSA)

LA-MRSA have emerged in farm animals mainly bovine, swine, companion animals and persons in contact with these animals [38]. Human infections associated with LA-MRSA have also been reported from several parts of the world [39]. Animal to human and vice versa of resistant strains can have a potential effect on public health if these strains enter into the community and health-care settings [40]. Transmission of bacterial species between humans and livestock is increasingly being detected in farmers and farm workers in Europe and much of the industrialized world [18]. Despite the fact that S. aureus is commonly associated with bovine mastitis, MRSA isolates have been infrequently recorded with the disease. There have been a few reports of MRSA colonization and/or infections in dairy cattle since the very first evidence of MRSA in mastitis in 1972 [41]. Recently, a highly divergent mecA gene (now named mecC) in a type XI SCCmec was found in bovine mastitis S. aureus [42]. Mastitic MRSA strains from different countries may share similar or different molecular traits. For example, reports from some European countries indicated that ST398 MRSA with SCCmec type IV or V played a vital part in clinical or subclinical bovine mastitis although it was not the only clonal line associated with mastitis [43]. Several genotypes including ST1/t286 MRSA with SCCmec type IVa, ST72/t324 MRSA with SCCmec type IV or IVa, and ST72/untypeable spa-type with SCCmec type IV were reported in Korea [44]. The majority of reported MRSA isolates in Turkey belonged to ST239/spa-type t30 with SCCmec type III, while others belonged to ST8/spa-type t190/SCCmec type IV, orST329/spa-type t30/SCCmec type III [45]. These data indicated that various MRSA clones were associated with bovine mastitis in different parts of the world.

The high incidence of methicillin resistance (47.6%) was recorded from S. aureus isolates of dairy farms in China [46]. As India is highest milk producer in the world, dairy production is one of the major agricultural activities among the people. Previous studies from northwest India and Chennai reported the MRSA positive percentages as 13% and 10.94% [47,48]. The higher percentage (19.23%) in this study clearly indicates improper antibiotic usage and poor intramammary administration of mastitis cows. The emergence of MRSA CC398 (known as LA-MRSA) in farm animals and human beings has shown that some S. aureus lines might not be strictly host-species restricted. MRSA ST398 can cause infection in people, with close animal contact being the main risk factor, suggesting that farm animals could provide a reservoir of MRSA [49]. Many antibiotic resistance genes are confirmed to have originated in microbes in the environment and have been transferred to other bacteria through MGEs, such as phages [50]. Cui et al. [51] reported the presence of MRSA in swine and swine farm workers in four Chinese provinces, all of which belonged to ST9 and spa type t899, possessed a type III SCCmec element and lacked the PVL gene. There was a report on MRSA from pet animals and veterinary staff in China, in which 22 MRSA isolates were distinguished using the API Staph-Ident System, MIC tests, and mecA-specific PCR assay [52]. Another study reported that MRSA of ST97 with SCCmec type IV, ST965 with SCCmec type IV, ST6 with SCCmec type IV, and ST9 with untypeable SCCmec were found in milk samples collected from bovine mastitis cases [53]. Saleha and Zunita [54] discussed the prevalence of MRSA and its impact on public health in Malaysia. They emphasized that there is a need to monitor the presence of MRSA in both pet and domestic animals similar to that in humans to prevent further spread of MRSA. Among 84 staphylococcal isolates were obtained from milk samples from cows, sheep, goats, and buffalo with subclinical mastitis in Rio de Janeiro State of Brazil, no host preference among the animal species have been recorded [55]. MRSA is prevalent in milk from semi-extensive dairy cows in northeastern Brazil, and further investigation on its extent in various types of milk production systems and the farm-to-table continuum is warranted [56].

Coagulase negative S. aureus (CoNS) as mastitic organisms in transmission of methicillin resistance

For instance in Finland, CoNS isolates were predominant from cows with clinical mastitis in which symptoms were most severe in cows with Staphylococcus hyicus infection [53]. Of note, human-CoNS species tend to be MDR yet their counterpart, S. aureus, is less prone to developing multi-resistance to antimicrobials particularly in the Nordic countries [54]. CoNS species from bovines in Europe are most of the time reported to be susceptible to antimicrobials [57]. Discrepancies in animal husbandry, management practices as well as enforcement of antimicrobial regulations are responsible for this. In veterinary medicine, CoNS have become a problem and are currently incriminated as causes in several recurrences of clinical mastitis.

S. epidermidis and Staphylococcus saprophyticus are members of CoNS, of that S. epidermidis is a common commensal in human skin and also contaminant in implants [58-60]. S. saprophyticus is an opportunistic pathogen, causing cystitis and uncomplicated urinary tract infections in women. Coagulase negative staphylococcus sp. might be common in subclinical mastitis also [61], but the presence of mecA in these species is a significant one. Jaglic et al. [62] reported that mecA was common in S. epidermidis (50%). The presence of methicillin resistance in S. epidermidis and S. saprophyticus implies sequel on environment. Methicillin-resistant S. saprophyticus was isolated from dairy products due to environmental contamination [63].

Transmission of MRSA between human and farm animals (Figure-1)

Figure-1
Possibility of methicillin-resistant Staphylococcus aureus transmission among human, dairy cow and environment.

Antoci et al. [64] evaluated the prevalence and molecular traits of MRSA among dairy farmers in the province of Ragusa, South-Eastern Sicily, their animals and bulk tank milk samples. The results were 36% of human nasal swabs, 61% of bovine nasal swabs, and 44% of bulk tank milk samples. The prevalence of MRSA carrier in humans significantly correlated with the percentage of positive cows on the farm, the number of livestock units, and the presence of positive bulk tank milk samples. Spohr et al. [65] found that milk samples of 5.1-16.7% of dairy cows were positive for MRSA; Virgin et al. [66] did not identify MRSA from bulk tank milk. Considering that the milking hygiene score correlated with the somatic cell count, which is increased in the presence of mastitis, it is supposable that the improvement of hygiene practices might reduce the risk for MRSA to spread on dairy farms, for example via milkers hands and milking clusters, which represent a common route of transmission for mastitis pathogens, especially S. aureus between cows. Prospective studies are needed to investigate MRSA transmission between animals and humans and implement preventive measures. Graveland et al. [67] mentioned the possibility of transmission between animals and people who are in close contact with them. A high rate of animal-to-human transmission of CC398 has been reported in pig farming, as well as a significant difference in MRSA prevalence between farmers and their families [68]. Köck et al. [69] found that contact with pigs was associated with the risk for MRSA CC398 colonization in a retrospective study among patients admitted to a tertiary-care university hospital. In addition to classical risk factors for MRSA carriage, Harbarth et al. [70] suggested to include the evaluation of contact with livestock as an additional risk factor to the admission screening schedule for hospitals, to identify subjects at higher risk for LA-MRSA colonization, who may be responsible for MRSA CC398 introduction in the nosocomial setting and may favor antimicrobial resistance import to hospitals. Prashanth et al. [71] assessed the genetic relationship of S. aureus isolated from cattle and human in India. In recent times, methicillin-resistant bacteria have been reported in wastewater treatment plants and environmental water samples as well. Since a large part of the antibiotics consumed by humans end up in wastewater, the antibiotics may exert selective pressure resulting in the emergence and transmission of the resistance-conferring genes in antibiotic susceptible organisms; it was proposed [72]. Nonetheless, the presence of β lactamase genes (blaTEM and bla CTX-M9) of Escherichia coli and mecA gene of MRSA in bacteriophages DNA isolated from environmental water samples, indicating that phages are reservoirs of resistance genes in the environment, implies that the horizontal gene transfer through MGEs such as plasmids, transposons or bacteriophages might be responsible for the presence of noticeable level of drug resistance in the environment [73-76]. 5 LA-MRSA isolates, 4 of which were obtained from skin and soft tissue infections, were identified from 3687 tested MRSA isolates from persons in Manitoba and Saskatchewan, Canada. Further molecular characterization determined that these isolates all contained SCCmec were negative for PVL and were closely related by macrorestriction analysis with the restriction enzyme Cfr91 [77]. Reports of antibiotic resistance from milk of mastitis-affected dairy farm have been tabulated as Table-1.

Table-1
Reports of antibiotic resistance from milk of mastitis affected dairy farm.

Measures to be monitored for avoiding transmission of antibiotic resistant infections

General clean public health, hygiene and sanitary measures should be followed. Development of culturally sensitive awareness campaigns, targeted to the general public, explaining the importance of protecting antibiotics and using them only when absolutely necessary. Provision of education about fundamental hygiene, such as hand-washing, to prevent the spread of infection. It is imperative to improve sanitation systems to eliminate resistant bacteria in wastewater [81].

Some of the WHO guidelines following for human treatment to avoid antibiotic resistance should be considered for animal treatment also. Once the etiology of the infection has been identified on the basis of reliable microbiological methods, antimicrobial therapy is directed at that pathogen. In addition to the constellation of suggestive clinical features, the standard treatment guidelines should include suggestion for diagnostic testing of the disease condition microbiological/pathological, hematological and biochemistry data/values. The recommendation should focus on the investigation for specific pathogens that would significantly alter standard management decisions. Recommendations are generally made for a class of antibiotics rather than a specific drug, unless outcome data clearly favors a specific drug. Since overall efficacy remains good for many classes of agents, the more potent drugs are given preference because of their benefit in decreasing the risk of selection for antibiotic resistance. Other factors for consideration of specific antimicrobials include pharmacokinetics/pharmacodynamics, compliance, safety, and cost. Recommendation on the use of antimicrobials should take into account the use of antimicrobials within the previous 3 months (in which case an alternative from a different class should be selected). In case the individual is from a geographical region that has a high rate (>25%) of resistant organisms reported or where high-level minimal inhibitory concentration is observed then, the use of alternative agents is mandatory [82-84].

Monitoring should be done to ascertain the following:

  • Is the clinical picture compatible with an infection?
  • Is there an indication for treatment with antibiotics?
  • Is the choice of the antimicrobial drug adequate?
    • Broadness of spectrum: Is the spectrum unnecessarily broad? Is the duration of treatment appropriate?
    • Too long/too short? Is the dosage correct?
    • Dose/interval/mode of administration. Is the timing appropriate?
    • Too early/too late?

When treating the animals attending in time, maintenance of treatment, transportation are some of the challenging factors. The veterinarians should have in mind all these while treating the animals with antibiotics.

The human health risks associated with consumption of raw or unpasteurized milk and milk products are well established and have been previously reviewed by Oliver et al. [85]. However, the enterotoxin of MRSA is very resistant to heating and pasteurization, boiling of the milk for 1 h leading to a decrease in the quantity of toxin but only autoclaving at 15 psi for 20 min being able to completely destroy the toxin. The sterilized milk needs to be refrigerated at 0°C to 4°C until further processing. Since staphylococci are known to grow well on saline media, the risk for contamination is higher with home-made salted cheeses [86].

Conclusion

The transmission of MRSA infections may be limited by universal infection-control measures, patient education, screening and decolonization of asymptomatic MRSA carriers in both health-care and community settings. Basic hygiene, good husbandry and biosecurity measures on farms, abattoirs, and food processing units have a tendency to reduce the spread of MRSA in animal population. Individuals with persistent animal contact should be educated on the risk of MRSA transmission in animals or their environment. Global initiatives are urgently needed to monitor the occurrence of and to assess risks posed by emerging clones. For CA-MRSA, emerging evidence suggests that current transmission of particular clones in local community and hospital settings is possible once imported from the Indian subcontinent. Consideration must be given to the screening of patients with a history of overseas travel or health-care contact for both resistant Enterobacteriaceae and Gram-positive bacteria, followed by appropriate infection control procedures. Today, the persistent, indiscriminate, and inappropriate use of antibiotics and the increasing specter of antibiotic resistance are an emerging unfavorable situation for health care. This state needs immediate action with current anti-infective therapies in India. Careful monitoring of the resistance status of S. aureus in dairy environments is required, as S. aureus transmission is dynamic and involves humans, animals, and likely the farm production environment. Further studies are essential to help identify critical areas that allow for contamination and spread within the farm environment.

Authors’ Contributions

SG and KCD: Conceived, analysed, drafted and revised the manuscript. Both authors read and approved the final manuscript.

Acknowledgments

Authors acknowledge Prof. R.M. Pitchappan and Prof. R. Murugesan, Chettinad Academy of Research and Educationfor their valuable suggestions.

Competing Interests

The authors declare that they have no competing interests.

References

1. Biedenbach D.J, Moet G.J, Jones R.N. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002) Diagn. Microbiol. Infect. Dis. 2004;50:59–69. [PubMed]
2. Barton M, Hawkes M, Moore D. Guidelines for the prevention and management of community associated methicillin resistant Staphylococcus aureus: Perspective for Canadian health care practitioners. Can. J. Infect. Dis. Med. Microbiol. 2006;17:4–24. [PMC free article] [PubMed]
3. Moon J.S, Lee A.R, Kang H.M, Lee E.S, Kim M.N, Paik Y.H, Park Y.H, Joo Y.S, Koo H.C. Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea. J. Diary Sci. 2007;90:1176–1185. [PubMed]
4. Quinn P.J, Carter M.E, Markey B, Carter G.R. Bacteriology, Staphylococcus species. St. Louis: Mosby, Harcourt Publishers Limited; 2000. Clinical Veterinary Microbiology; pp. 118–126.
5. Gordon R.J, Lowry F.D. Pathogenesis of methicillin resistant Staphylococcus aureus infection. Clin. Infect. Dis. 2008;46:350–359. [PMC free article] [PubMed]
6. Livermore D.M. Antibiotic resistance in staphylococci. Intl. J. Antimicrob. Agents. 2000;16:3–10. [PubMed]
7. Jevons M.P. “Calbenin”-resistant staphylococci. Br. Med. J. 1961;1:124–125.
8. Deurenberg R.H, Vink C, Kalenic S, Friedrich A.W, Bruggeman C.A, Stobberingh E.E. The molecular evolution of methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 2007;13:222–235. [PubMed]
9. Juuti K, Ibrahem S, Virolainen-Julkunen A, Vuopio-Varkila J, Kuusela P. The pls gene found in methicillin-resistant Staphylococcus aureus strains is common in clinical isolates of Staphylococcus sciuri. J. Clin. Microbiol. 2005;43:1415–1419. [PMC free article] [PubMed]
10. Katayama Y, Takeuchi F, Ito T, Ma X.X, Ui-Mizutani Y, Kobayashi I, Hiramatsu K. Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus. J. Bacteriol. 2003;185:2711–2722. [PMC free article] [PubMed]
11. Wisplinghoff H, Rosato A.E, Enright M.C, Noto M, Craig W, Archer G.L. Related clones containing SCCmec type IV predominate among clinically significant Staphylococcus epidermidis isolates. Antimicrob. Agents Chemother. 2003;47:3574–3579. [PMC free article] [PubMed]
12. Hanssen A.M, Sollid J.U. Multiple staphylococcal cassette chromosomes and allelic variants of cassette chromosome recombinases in Staphylococcus aureus and coagulase-negative staphylococci from Norway. Antimicrob. Agents Chemother. 2007;51:1671–1677. [PMC free article] [PubMed]
13. Ito T, Hiramatsu K. Acquisition of methicillin resistance and progression of multiantibiotic resistance in methicillin-resistant Staphylococcus aureus. Yonsei Med. J. 1998;39:526–533. [PubMed]
14. Klein E, Smith D.L, Laxminarayan R. Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States 1999-2005. Emerg. Infect. Dis. 2007;13:1840–1846. [PMC free article] [PubMed]
15. Devriese L.A, Hommez J. Epidemiology of methicillin-resistant Staphylococcus aureus in dairy herds. Res. Vet. Sci. 1975;19:23–27. [PubMed]
16. Otter J.A, French G.L. Molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus in Europe. Lancet Infect. Dis. 2010;10:227–239. [PubMed]
17. De Lencastre H, Oliveira D, Tomasz A. Antibiotic resistant Staphylococcus aureus: A paradigm of adaptive power. Curr. Opin. Microbiol. 2007;10:428–435. [PMC free article] [PubMed]
18. Chambers H.F, Deleo F.R. Waves of resistance in the antibiotic era. Nat. Rev. Microbiol. 2009;7:629–641. [PMC free article] [PubMed]
19. Joshi S, Ray P, Manchanda V, Bajaj J, Chitnis DS, Gautam V. Methicillin resistant Staphylococcus aureus (MRSA) in India: Prevalence and susceptibility pattern. Indian. J. Med. Res. 2013;137:363–9. [PMC free article] [PubMed]
20. Centers for Disease Control and Prevention. (2002) Methicillin resistant Staphylococcus aureus skin or soft tissue infections in a state prison—Mississippi. JAMA. 287:181–182. [PubMed]
21. Abudu L, Blair I, Fraise A, Cheng K.K. Methicillin-resistant Staphylococcus aureus (MRSA): A community-based prevalence survey. Epidemiol. Infect. 2001;126:351–356. [PubMed]
22. Adhikari R.P, Cook G.M, Lamont I, Lang S, Heffernan H, Smith J.M. Phenotypic and molecular characterization of community occurring, Western Samoan phage pattern methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 2002;50:825–831. [PubMed]
23. Centers for Disease Control and Prevention. Public health dispatch: Outbreaks of community-associated methicillin resistant Staphylococcus aureus skin infections—Los Angeles County, California 2002-2003. JAMA. 2003;289:1377. [PubMed]
24. Centers for Disease Control and Prevention. Methicillin resistant Staphylococcus aureus infections among competitive sports participants—Colorado, Indiana, Pennsylvania, and Los Angeles County 2000-2003. MMWR Morb. Mortal. Wkly. Rep. 2003;52:793–795. [PubMed]
25. Bansal S, Kashyap S, Pal L.S, Goel A. Clinical and bacteriological profile of community acquired pneumonia in Shimla, Himachal Pradesh. Indian J. Chest. Dis. Allied Sci. 2004;46:17–22. [PubMed]
26. Groom A.V, Wolsey D.H, Naimi T.S, Smith K, Johnson S, Boxrud D, Moore K.A, Cheek J.E. Community-acquired methicillin-resistant Staphylococcus aureus in a rural American Indian community. JAMA. 2001;286:1201–1205. [PubMed]
27. Mohanasundaram K.M, Lalitha M.K. Comparison of phenotypic versus genotypic methodsin the detection of methicillin resistance in Staphylococcus aureus. Indian J. Med. Res. 2008;127:78–84. [PubMed]
28. Alvarez-Uria G, Reddy R. Prevalence and antibiotic susceptibility of community-associated methicillin-resistant Staphylococcus aureus in a rural area of India: Is MRSA replacing methicillin-susceptible Staphylococcus aureus in the community? ISRN Dermatol 2012. 2012:248951. [PMC free article] [PubMed]
29. D’Souza N, Rodrigues C, Mehta A. Molecular characterization of methicillin-resistant Staphylococcus aureus with emergence of epidemic clones of sequence type (ST) 22 and ST 772 in Mumbai, India. J. Clin. Microbiol. 2010;48:1806–1811. [PMC free article] [PubMed]
30. Teixeira L.A, Resende C.A, Ormonde L.R, Rosenbaum R, Figueiredo A.M, de Lencastre H, Tomasz A. Geographic spread of epidemic multiresistant Staphylococcus aureus clone in Brazil. J. Clin. Microbiol. 1995;33:2400–2404. [PMC free article] [PubMed]
31. Vivoni A.M, Diep B.A, de Gouveia Magalhães A.C, Santos K.R, Riley L.W, Sensabaugh G.F, Moreira B.M. Clonal composition of Staphylococcus aureus isolates at a Brazilian university hospital: Identification of international circulating lineages. J. Clin. Microbiol. 2006;44:1686–1691. [PMC free article] [PubMed]
32. Chatterjee S.S, Ray P, Aggarwal A, Das A, Sharma M. A community based study on nasal carriage of Staphylococcus aureus. Indian J. Med. Res. 2009;130:742–748. [PubMed]
33. Rajaduraipandi K, Mani K.R, Panneerselvam K, Mani M, Bhaskar M, Manikandan P. Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus: A multicentre study. Indian J. Med. Microbiol. 2006;24:34–38. [PubMed]
34. Rybak M.J, LaPlante K.L. Community-associated methicillin-resistant Staphylococcus aureus: A review. Pharmacotherapy. 2005;25(1):74–85. [PubMed]
35. Brennan G.I, Shore A.C, Corcoran S, Tecklenborg S, Coleman D.C, O’Connell B. Emergence of hospital-and community-associated panton-valentine leukocidin-positive methicillin-resistant Staphylococcus aureus genotype ST772-MRSA-V in Ireland and detailed investigation of an ST772-MRSA-V cluster in a neonatal intensive care unit. J. Clin. Microbiol. 2012;50:841–847. [PMC free article] [PubMed]
36. Yamamoto T, Takano T, Yabe S, Higuchi W, Iwao Y, Isobe H, Ozaki K, Takano M, Reva I, Nishiyama A. Super-sticky familial infections caused by panton-valentine leukocidin-positive ST22 community-acquired methxsistant Staphylococcus aureus in Japan. J. Infect. Chemother. 2012;18:187–198. [PubMed]
37. Pinto A.N, Seth R, Zhou F, Tallon J, Dempsey K, Tracy M, Gilbert G.L, O’Sullivan M.V. Emergence and control of an outbreak of infections due to panton-valentine leukocidin positive, ST22 methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit. Clin. Microbiol. Infect. 2013;19:620–627. [PubMed]
38. Cuny C, Friedrich A, Kozytska S, Layer F, Nubel U, Ohlsen K, Strommenger B, Walther B, Wieler L, Witte W. Emergence of methicillin-resistant Staphylococcus aureus (MRSA) in different animal species. Int. J. Med. Microbiol. 2010;300:109–117. [PubMed]
39. Van Cleef B.A, Monnet D.L, Voss A, Krziwanek K, Allerberger F, Struelens M. Livestock associated methicillin-resistant Staphylococcus aureus in humans, Europe. Emerg. Infect. Dis. 2011;17:502–523. [PMC free article] [PubMed]
40. Fitzgerald J.R. Human origin for livestock-associated methicillin-resistant Staphylococcus aureus. mBio. 2012;3(2):e00082–12. [PMC free article] [PubMed]
41. Huber H, Koller S, Glezendanner N, Stephan R, Zweifel C. Prevalence and characteristics of methicillin-resistant Staphylococcus aureus in humans in contact with farm animals, in livestock, and in food of animal origin, Switzerland 2009. Euro Surveill. 2010;15:19542. [PubMed]
42. Paterson G.K, Morgan F.J.E, Harrison E.M, Peacock S.J, Parkhill J, Zadoks R.N, Holmes M.A. Prevalence and properties of mecC methicillin-resistant Staphylococcus aureus (MRSA) in bovine bulk tank milk in Great Britain. Antimicrob. Agents Chemother. 2014;69(3):598–602. [PMC free article] [PubMed]
43. Holmes M.A, Zadoks R.N. Methicillin resistant S. aureus in human and bovine mastitis. J. Mammary Gland Biol. Neoplasia. 2011;16:373–382. [PubMed]
44. Nam H.M, Lee A.L, Jung S.C, Kim M.N, Jang G.C, Wee S.H, Lim S.K. Antimicrobial susceptibility of Staphylococcus aureus and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Korea. Foodborne Pathog. Dis. 2011;8:231–238. [PubMed]
45. Tu¨rkyilmaz S, Tekbiyik S, Oryasin E, Bozdogan B. Molecular epidemiology and antimicrobial resistance mechanisms of methicillin-resistant Staphylococcus aureus isolated from bovine milk. Zoonoses Public Health. 2010;57:197–203. [PubMed]
46. Pu W, Su Y, Li J, Li C, Yang Z, Deng H, Ni C. High incidence of oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) associated with bovine mastitis in China. PLoS One. 2014;9(2):e88134. [PMC free article] [PubMed]
47. Kumar R, Yadav B.R, Singh R.S. Antibiotic resistance and pathogenicity factors in Staphylococcus aureus isolated from mastitic Sahiwal cattle. J. Biosci. 2011;36:175–188. [PubMed]
48. Chandrasekaran D, Venkatesan P, Tirumurugaan K.G, Gowri B, Subapriya S, Thirunavukkarasu S. Sub-acute mastitis associated with methicillin resistant Staphylococcus aureus in a cow: A case report. J. Adv. Vet. Anim. Res. 2014;1(4):235–237.
49. Garcia-Alvarez L, Holden M.T.G, Lindsay H, Webb C.R, Brown D.F.J, Curran M.D, Walpole E, Brooks K, Pickard D.J, Teale C, Parkhill J, Bentley S.D, Edwards G.F, Girvan E.K, Kearns A.M, Pichon B, Hill R.L, Larsen A.R, Skov R.L, Peacock S.J, Maskell D.J, Holmes M.A. Methicillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine population in the UK and Denmark: A descriptive study. Lancet Infect. Dis. 2011;11:595–603. [PMC free article] [PubMed]
50. Wright G.D. Antibiotic resistance in the environment: A link to the clinic? Curr. Opin. Microbiol. 2010;13:589–594. [PubMed]
51. Cui S.H, Li J.Y, Hu C.Q, Jin S.H, Li F.Q, Guo Y, Ran L, Ma Y. Isolation and characterization of methicillin-resistant Staphylococcus aureus from swine and works in China. Antimicrob. Agents Chemother. 2009;64:680–683. [PubMed]
52. Zhang W.J, Hao Z.H, Wang Y, Cao X.Y, Logue C.M, Wang B, Yang J, Shen J, Wu C. Molecular characterization of methicillin-resistant Staphylococcus aureus from pet animals and veterinary staff in China. Vet. J. 2011;190:e125–e129. [PubMed]
53. Wang D.F, Duan X.H, Wu J.Y, Yang X.Y, Li J.J, Zhi-Cai W. The current status of the drug resistance and evolutionary relationship of MSSA and MRSA isolates from bovine of China. Acta Vet. Zootech. Sin. 2011;42:1416–1425.
54. Saleha A.A, Zunita Z. Methicillin resistant Staphylococcus aureus (MRSA): An emerging veterinary and zoonotic pathogen of public health concern and some studies in Malaysia. J. Anim. Vet. Adv. 2010;9(7):1094–1098.
55. Aires-de-Sousa M, Parente C.E.S, Vieira-da-Motta O, Bonna I.C.F, Silva D.A, de Lencastre H. Characterization of Staphylococcus aureus isolated from buffalo, bovine, ovine, and caprine milk samples from Rio de Janeiro State, Brazil. Appl. Environ. Microbiol. 2007;73(12):3845–3849. [PMC free article] [PubMed]
56. Oliveira C.J.B, Tiao N, de Sousa F.G.C, de Moura J.F.P, Santos Filho L, Gebreyes W.A. Methicillin-resistant Staphylococcus aureus from Brazilian dairy farms and identification of novel sequence types. Zoonoses Public Health. 2016;63:97–105. [PubMed]
57. Honkanen-Buzalski T, Myllys V, Pyörälä S. Bovine clinical mastitis due to coagulase-negative staphylococci and their susceptibility to antimicrobials. Zentralbl. Veterinarmed. B. 1994;41(5):344–350. [PubMed]
58. Hendriksen R.S, Mevius D.J, Schroeter A, Teale C, Meunier D, Butaye P, Franco A, Utinane A, Amado A, Moreno M, Greko C, Stärk K, Berghold C, Myllyniemi A.L, Wasyl D, Sunde M, Aarestrup F.M. Prevalence of antimicrobial resistance among bacterial pathogens isolated from cattle in different European countries 2002-2004. Acta Vet. Scand. 2008;50:28. [PMC free article] [PubMed]
59. Fessler A.T, Billerbeck C, Kadlec K, Schwarz S. Identification and characterization of methicillin-resistant coagulase-negative staphylococci from bovine mastitis. J. Antimicrob. Chemother. 2010;65:1576–1582. [PubMed]
60. Foster T. Staphylococcus. In: Barron S, editor. Barron’s Medical Microbiology. 4th ed. Los Angeles: University of Texas Medical Branch; 1996.
61. Cervinkova D, Babak V, Marosevic D, Kubikova I, Jaglic Z. The role of the qacA gene in mediating resistance to quaternary ammonium compounds. Microb. Drug Resist. 2013;19:160–167. [PubMed]
62. Jaglic Z, Michu E, Holasova M, Vlkova H, Babak V, Kolar M, Bardon J, Schlegelova J. Epidemiology and characterization of Staphylococcus epidermidis isolates from humans, raw bovine milk and a dairy plant. Epidemiol. Infect. 2010;138:772–782. [PubMed]
63. Zouhairi O, Saleh I, Alwan N, Toufeili I, Barbour E, Harakeh S. Antimicrobial resistance of Staphylococcus species isolated from Lebanese dairy-based products. East Mediterr. Health. 2010;16(12):1221–1225. [PubMed]
64. Antoci E, Pinzone M.R, Nunnari G, Stefani S, Cacopardo B. Prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) among subjects working on bovine dairy farms. Infez. Med. 2013;21:125–129. [PubMed]
65. Spohr M, Rau J, Friedrich A, Klittich G, Fetsch A, Guerra B, Hammerl J.A, Tenhagen B.A. Methicillin resistant Staphylococcus aureus (MRSA) in three dairy herds in Southwest Germany. Zoonoses Public Health. 2011;58:252–261. [PubMed]
66. Virgin J.E, Van Slyke T.M, Lombard J.E, Zadoks R.N. Methicillin-resistant Staphylococcus aureus detection in US bulk tank milk. J. Dairy Sci. 2009;92:4988–4991. [PubMed]
67. Graveland H, Wagenaar J.A, Bergs K, Heesterbeek H, Heederik D. Persistence of livestock associated MRSA CC398 in humans is dependent on intensity of animal contact. PLoS One. 2011;6(2):e16830. [PMC free article] [PubMed]
68. Crombe F, Vanderhaeghen W, Dewulf J, Hermans K, Haesebrouck F, Butaye P. Colonization and transmission of methicillin-resistant Staphylococcus aureus ST398 in nursery piglets. Appl. Environ. Microbiol. 2012;78(5):1631–1634. [PMC free article] [PubMed]
69. Köck R, Brakensiek L, Mellmann A, Kipp F, Henderikx M, Harmsen D, Daniels-Haardt I, von Eiff C, Becker K, Hendrix M.G, Friedrich A.W. Cross-border comparison of the admission prevalence and clonal structure of methicillin-resistant Staphylococcus aureus. J. Hosp. Infect. 2009;71:320–326. [PubMed]
70. Harbarth S, Sax H, Fankhauser-Rodriguez C, Schrenzel J, Agostinho A, Pittet D. Evaluating the probability of previously unknown carriage of MRSA at hospital admission. Am. J. Med. 2006;119(275):e15–e23. [PubMed]
71. Prashanth K, Rajender Rao P.V, Vivek Reddy R, Saranathan A.R. Genotypic characterization of Staphylococcus aureus obtained from humans and bovine mastitis samples in India. J. Glob. Infect. Dis. 2011;3(2):115–122. [PMC free article] [PubMed]
72. Goldstein R.E.R, Micallef S.A, Gibbs S.G, Davis J.A, He X, George A, Kleinfelter L.M, Schreiber N.A, Mukherjee S, Sapkota A, Joseph S.W, Sapkota A.R. Methicillin-resistant Staphylococcus aureus (MRSA) detected at four U.S. Wastewater treatment plants. Environ. Health Perspect. 2012;120:11. [PMC free article] [PubMed]
73. Canton R. Antibiotic resistance genes from the environment: A perspective through newly identified antibiotic resistance mechanisms in clinical setting. Eur. Soc. Clin. Microbiol. Infect. Dis. 2009;15(1):20–25. [PubMed]
74. Muniesa M, García A, Miró E, Mirelis B, Prats G, Jofre J, Navarro F. Bacteriophages and diffusion of beta-lactamase genes. Emerg. Infect. Dis. 2004;10:1134–1137. [PMC free article] [PubMed]
75. Martinez J.L. Antibiotics and antibiotic resistance genes in natural environments. Science. 2008;321:365–367. [PubMed]
76. Colomer-Lluch M, Jofre J, Muniesa M. Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples. PLoS One. 2011;6:e17549. [PMC free article] [PubMed]
77. Golding G.R, Bryden L, Levett P.N, McDonald R.R, Wong A, Wylie J, Graham M.R, Tyler S, Van Domselaar G, Simor A.E, Gravel D, Mulvey M.R. Livestock-associated methicillin-resistant Staphylococcus aureus sequence type 398 in humans, Canada. Emerg. Infect. Dis. 2010;16:587–594. [PMC free article] [PubMed]
78. Kwon N.H, Park K.T, Moon J.S, Jung W.K, Kim S.H, Kim J.M, Hong S.K, Koo H.C, Joo Y.S, Park Y.H. Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J. Antimicrob. Chemother. 2005;56:624–632. [PubMed]
79. Rabello R.F, Moreira B.M, Lopes R.M.M, Teixeira L.M, Riley L.W, Castro A.C. Multilocus sequence typing of Staphylococcus aureus isolates recovered from cows with mastitis in Brazilian dairy herds. J. Med. Microbiol. 2007;56:1505–1511. [PubMed]
80. McKay A.M. Antimicrobial resistance and heat sensitivity of oxacillin-resistant, mecA-positive Staphylococcus spp. From unpasteurized milk. J. Food Prot. 2008;71(1):186–190. [PubMed]
81. Economou V, Gousia P. Agriculture and food animals as a source of antimicrobial-resistant bacteria. Infect. Drug Resist. 2015;8:49–61. [PMC free article] [PubMed]
82. National Policy for Containment of Antimicrobial Resistance, India. Directorate General of Health Services, Ministry of Health & Family Welfare. 2011
83. Bhagwati A. Guidelines for antibiotic usage in common situations. JAPI. 2010;58:49–50. [PubMed]
84. Balaji V, Abraham O.C, Sujith C, Surang D, Vikas M, Sangeeta J, Anuj S, Rajesh B. Step-by-Step Approach for Development and Implementation of Hospital Antibiotic Policy and Standard Treatment Guidelines. New Delhi: World Health Organization, SEA-HLM; 2011. p. 414.
85. Oliver S.P, Murinda S.E, Jayarao B.M. Impact of antibiotic use in adult dairy cows on antimicrobial resistance of veterinary and human pathogens: A comprehensive review. Foodborne Pathog. Dis. 2011;8(3):337–355. [PubMed]
86. Dhanashekar R, Akkinepalli S, Nellutla A. Milk-borne infections. An analysis of their potential effect on the milk industry. GERMS. 2012;2(3):101–109. [PMC free article] [PubMed]

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