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The intense focus on studying human height has done more than any other genetic analysis to advance our understanding of the heritability of highly complex phenotypes. Here, we describe in detail the properties of a previously unexplored trait in Drosophila melanogaster that shares many salient properties with human height. The total length of the pupal case varies between 2.8 and 3.9 mm among natural variants, and we report that it is among the most heritable traits reported in this species. We have developed a simple semiautomatic phenotyping system with which a single operator can reliably score >5000 individuals in a day. The precision of the automated system is 0.042 mm (± 0.030 SD). All phenotyped individuals are available to be mated in subsequent generations or uniquely archived for future molecular work. We report both broad sense and narrow sense heritability estimates for two biologically distinct data sets. Narrow sense heritability (h2) ranged from 0.44 to 0.50, and broad sense heritability (H2) ranged from 0.58 to 0.61. We present results for mapping the trait in 195 recombinant inbred lines, which suggests that there are no loci with >10% effect size in this panel. We propose that pupal size genetics in Drosophila could represent a model complex trait amenable to deep genetic dissection using the automated system described.
Elucidating the genetic architecture of a phenotypic trait fundamentally requires that it is, to some degree, heritable. Phenotypic traits with low heritability do not generally require additional explanation as to why quantitative trait loci (QTL) cannot be robustly identified. However, while traits with high heritability increase the power to identify causative loci, the genetic architecture of traits remains a key factor in predicting success in identifying QTL. For example, a highly heritable trait that is dependent on a large number of interacting alleles or loci may still require substantial sample sizes and complex analyses to identify causative loci (Visscher et al. 2010; Barton et al. 2016). The archetypical example of this is human height, which, by at least two distinct measures of heritability, is among the most heritable of quantitative human traits, h2 ≈ 0.4–0.7 using regression of trios (Galton 1886; Hanley 2004; Visscher et al. 2010) or h2 ≈ 0.7 using twin studies (Polderman et al. 2015). However, the identification of the loci involved, and any interactions among them, has proven far from trivial, and successes to date have been dependent on utilizing very large data sets [up to 250,000 individuals (Wood et al. 2014)]. The current understanding being that of the 180–4000 loci implicated in impacting human height over its typical range all have additive effect sizes of <<1 mm (Weedon et al. 2008; Visscher et al. 2010; Wood et al. 2014). Although studies of human height represent a valuable model with which to test how complex heritable phenotypes can be dissected, the ability to experimentally control allele frequencies and environmental conditions would be a valuable capacity that is not possible in human studies. While this is a possibility in model organisms, there are relatively few morphological traits that have been robustly demonstrated to be both highly heritable and amenable to automated high throughput phenotyping (though see below). Herein, we describe in detail a new quantitative trait—length of the pupal case—in Drosophila melanogaster that places it in the 15–20% most heritable morphological traits described in this species, and is amenable to reliable and automated high-throughput phenotyping (Roff and Mousseau 1987).
The utility of automated phenotyping systems for a wide variety of organisms has increased greatly over the last 10 yr. While automated morphological phenotyping systems have been developed for Drosophila, as yet only automation analysis of images of wings has been widely employed (Houle et al. 2003). However, acquiring the wing images requires manual manipulation to position flies individually and so is difficult to scale up. Likewise, a system to measure heartbeat function requires that each fly be manipulated into position. An alternative approach to phenotyping in a selection experiment for gross body size was achieved using a series of graduated sieves (Turner et al. 2011). More recently, a sophisticated platform called the “fly cat walk” was described that has the capacity to reliably phenotype 700 flies a day for a wide range of morphological traits simultaneously (Medici et al. 2015). This requires no user manipulation of individual flies and is nondestructive. While the cost of constructing the equipment is not detailed (https://github.com/IMSB/FlyCatwalk/), it is likely that it would represent a significant investment of time, expertise, and resources for most laboratories.
Our setup uses an inexpensive camera in a light-proof box and the open source image analysis software Cellprofiler, with which a single user can phenotype 5000 pupae in a day. Pupae are photographed in situ on flattened squares of transparent film that lined the entire vertical surface of the vials. We demonstrate how the system can be used to (1) measure panels of recombinant inbred lines (RILs), and (2) generate large numbers of parent offspring trios that are particularly useful in exploring complex traits using artificial selection techniques. Furthermore, the increased throughput facilitates exploring the heritability of family means rather than single individual measurements, which are associated with increased measurement variance. Hence, pupal size could become a model phenotype that will allow deep dissection of its genetic architecture, since the availability of automated phenotyping will allow to screen very large mapping panels, or to design new complex mapping strategies.
The automated phenotyping system was applied to two independent datasets.
The first dataset, referred to here as “eight-way” is a collection of 195 RILs that are part of the Drosophila Synthetic Population Resource (http://wfitch.bio.uci.edu/~dspr/). These RILs are all originally derived from a cross between eight global stocks of D. melanogaster, their generation is described in detail in King et al. (2012a,b). Narrow sense heritability, h2, for the eight-way dataset was estimated by measuring the progeny of 67 single pair matings between individuals from 11 different RILs that spanned the full range of phenotype measurements (IDs of crossed stocks are given in Supplemental Material, File S5; six of the 67 crosses are duplicates also indicated in File S5). Broad sense heritability estimates H2 of the eight-way dataset were generated by repeated measurements of RILs (IDs given in File S5).
The second dataset, termed “four-way,” was initiated by a cross between two Japanese and two African stocks (see Table S1). Narrow sense heritability, h2, was estimated by measuring the progeny of single pair matings from the 2nd to 6th generation where phenotyped parents were selected randomly from different vials to form subsequent generations. The following number of single pairs were measured in each generation [G2, 15 pairs], [G3, 81 pairs], [G4, 78 pairs], [G5, 88 pairs], and [G6, 154 pairs]. No duplicate vials were generated from the same mating pair. Note that, unlike the eight-way dataset, levels of heterozygosity in the parents and their offspring are likely to be similar. H2 estimates of the four-way dataset were generated by measuring 83 RILs established from the four-way cross by generation 37 (see File S5 for stock IDs). Details of all progenitor stocks of the four-way, and four-way datasets with estimates of their pupal length, are given in Table S1.
Flies were maintained on standard food dispensed into 28.5 mm diameter, 95 mm height vials (Genesee Scientific). Once the food vials had fully cooled, 10 cm × 10.5 cm squares of overhead projector film were slid into each vial lining their entire vertical wall (nobo, plain paper copier film, 33638237). A more detailed description of the entire procedure and equipment set up is provided as File S1. A custom printed semitransparent label, including a unique barcode, was affixed to the outside of each vial. Vials were incubated at 24° in a 12 hr light/12 hr dark incubator. Adults were removed after one to two nights in vials (sometimes three to four nights, if fertility appeared to be low, or due to holidays). Generally, by the 10th day after the parents were initially introduced, the majority of offspring in the vials were present as pupae attached to the transparent film; few if any larvae remained in the food. The film from each vial was removed, and placed into a purpose-made plastic frame (this frame can be 3D printed using a file provided as File S2) that holds the film flat for photographing. Food from the lower part of the film was scraped away and any larvae, or any white puparium stage (P1), removed. The frame was then photographed using bottom illumination in a light tight box. Batches of the resulting images were then analyzed using the procedure below.
A Cellprofiler (v2.1.0) pipeline was developed to simultaneously recognize pupae and to measure a variety of attributes, including length. The outputs of all measurements are written to .xls files that can then be viewed or imported into any database program. Cellprofiler is free, open access, software providing a suite of flexible image analysis tools (Lamprecht et al. 2007). In brief, the Cellprofiler pipeline first identifies “primary objects” distinct from the background without restriction on their size (module: identify primary objects). Then, applying a scalable model of pupal shape to all objects, those that are composed of multiple touching pupae are separated into distinct pupae [module: Untangle Worms, (Wählby et al. 2012)]. The resulting putative pupae are then each shrunk, and then repropagated outwards to more precisely identify the edges of each pupa based on boundary changes in pixel intensity (module: Identify Secondary objects). Finally, pupae are crudely filtered on size attributes and the proximity of neighboring objects to place them in one of the three confidence classes described in the Results. The digital outlines of pupae are overlaid onto a cropped version of the original image to allow users to easily visually assess quality.
Overlaid images and files of measurements were imported in batches into the database program FileMaker (v14, FileMaker Inc.). A unique barcode sticker identifying the film in the image was automatically read by the database. This enabled the image files to be automatically renamed with the barcode as their name for archiving. In addition to pupal measurements, a 1 €cent coin (16.25 mm diameter) present in all images was measured to control for camera changes, and to allow conversion of measurement in pixels to millimeter. Quality filtering of pupae and basic analyses were performed using the database. An annotated copy of the Cellprofiler pipeline is provided as File S3.
H2 was estimated using SPSS version 22 with the “variance components” function, with pupal length as the dependent variable, and RIL name as a random factor. This was done using the Minimum Norm Quadratic Unbiased method (though ANOVA produced identical results to two decimal places). The model for the single factor was yij = μ + αj +eij, where yij is the jth observation of the ith RIL, μ is the overall mean, αj is the random factor, and e is the associated error.
Estimates of h2 were made using the “linear regression” function, with pupal length as the dependent variable, and parent-midpoints as an independent variable. All other statistical analyses and graphs were also performed using SPSS or Filemaker. All data for individual pupa are available as File S4, and for vial means as File S5 (fields used in each graph are indicated in the associated “readme” files).
Estimates of h2 for human height data were made using the same methods as for pupae, using the original data of Francis Galton for 898 individuals, transcribed from his 1880s laboratory notebooks (Hanley 2004) (available http://www.math.uah.edu/stat/data/Galton.html). The 760 individuals in 123 families where four or more individuals were measured were used for estimates of the h2 heritability of mean family height (females were not transmuted into males).
Scans were performed in R (version 3.2.2) for the 195 DSPR RILs mentioned above (King et al. 2012a,b) using the “DSPRscan” command within the DSPRqtl-tools procedures described in King et al. (2012a,b). These utilize one SNP per 10 kb throughout the genome reference (with the exception of the Y chromosome and the mitochondrial genome). Significance LOD score thresholds were estimated using the “DSPRperm” command with 1000 replicates. The data input file is available as File S6. This scan was undertaken to asses whether one or few loci of large effect control pupal length, i.e., the trait is not complex. Note that, if the trait is controlled by many loci of small or moderate effect size, the scan is too underpowered to realistically identify individual loci. However, the goal of the scan is to show that pupal size is not a relatively simple trait controlled by only a few major effect loci.
The large numbers of individuals and vials measured, made possible through the use of the automated measuring system, provides robust insights into how phenotypic variation is partitioned for pupal length. While the method automatically identifies and measures pupae for multiple different parameters (see File S1 for a full list), the length of the pupae was the principle focus of this study. Two distinct sets of biological material were used to define the biological properties of pupal length. The first dataset was all derived from an eight-way cross (King et al. 2012a,b); the second was from a four-way cross. None of the founding stocks are shared between the eight-way and the four-way datasets. H2 (reflecting all potentially genetic contributions to trait variance e.g., additive, epistatic, dominance, maternal, and paternal effects) was estimated through the analysis of repeated measurements of RILs, whereas h2 heritability (reflecting only the impact of additive genetic effects) was measured by regression of midparent against their offspring measurements. Sampling properties of the eight-way and four-way datasets are detailed in Table 1 and Figure 1 (see also Figure S1 for summary of repeat measurements of RILs).
The automated pupal recognition pipeline attempts to identify the external outlines of three classes of objects:
Of the pupae retained for analysis, 47 and 53% were high and medium confidence, respectively. The probability of pupae being manually excluded as aberrantly measured was 0.02 for high quality pupae and 0.10 for medium confidence pupae (Figure 2). Note that not all images were examined manually, as, due to the robustness of the automated estimates, generally only vials with atypically high variance of the mean were examined.
The precision of the automated system was assessed by remeasuring a subset of films after rotating them by 180°, and comparing 516 duplicated measurements of the same pupae (Figure 3). The difference between the two measurements was an average of 0.043 mm (±0.030 SD). Remeasuring pupae after delays of 15 or 30 hr also generated similar precision estimates, indicating that, once pupae become brown puparium (P2), there is no detectable change in pupal length. Furthermore, the exuvia of eclosed individuals can also be reliably measured (data not shown).
Unsurprisingly, not all pupae were correctly captured by the automated system, and, based on a sample of 148 vials, an average 20% ± 11.7 SD pupae per vial were missed, called as low confidence objects, or manually excluded (Figure 4 and Figure 2). While there is an increased variance in the proportion of unmeasured pupae in higher density vials (e.g., where the automated count is >80), even here the automated count provides a reasonable proxy for density of individuals in each vial. This assumes that few, if any, larvae remained on the food surface after film transfer, as was generally the case (due in part to the short period parents remained in the vials). In addition, the small number of pupae removed from films that were obscured by larval food represents a constant proportion across all vials (see File S1).
One common major environmental covariate of many Drosophila traits is density of individuals within the vial in which they develop. Consequently, many Drosophila researchers control for this in experiments by collecting large numbers of zygotes and placing a controlled number in each vial. This is a fairly laborious process to perform routinely, and is complicated considerably where single pair crosses are required.
Here, density was controlled only indirectly through limiting the number of parents used per vial, and restricting the number of nights they remained before being cleared [generally two nights for single pair crosses, and one night for small groups (n = 10–20) of RIL individuals, see File S1]. Throughout all experiments, two stocks were continually remeasured to act as controls (stock 335 and 329, see Table S1). The large number of repeat measurements of these two stocks across a range of densities permits the examination of any relationship between density and pupal length (Figure 5). Furthermore, in the same way, it is also possible to explore the relationship between density and pupal length using the repeated eight-way and four-way RIL measurements (see Figure S2). All RILs and control stocks exhibit a uniformly negative relationship between density and pupal length; the slope varies from −0.0006 to −0.0041. This observed variability may reflect either variance in estimating slopes, or also that RILs exhibit different reaction norms. If the mode slope of −0.002 (Figure S2) is used to correct the mean length of vials (or individuals) to that observed at the mean observed vial density, this can be achieved with the following equation:
Applying this formula to the 431 vials established as single pair crosses, >99% of them would require a correction of <±0.1 mm, and, of the 1620 vials established from small groups of RIL individuals, 95% would require a correction of <±0.118 mm (Figure 6). Given the modest number of vials subjected to a large correction, and uncertainly about whether there is a truly universal linear relationship between density and pupal length, none of the measurements presented in the manuscript have been corrected analytically for density. Furthermore, with respect to repeated RIL measurements, reducing any confounding impact of density can be achieved by either experimentally increasing the number of replicate measurements closer to the mean density, or, analytically, by excluding or weighting down vials with extreme density values.
A script was written in Filemaker to explore the extent to which estimates of mean vial pupal length based on subsamples of individuals within a vial deviate from the vial means based on all individuals. This provides insight into at what point vials with low densities generate mean estimates with unacceptably high variance. Figure 7 indicates that selecting single individuals to estimate vial means unsurprisingly results in a high degree of variance of up to 0.4 mm. While these estimates are unbiased, this is likely to prove unacceptably high given that the total observed range of pupal lengths is 1.1 and 0.8–0.9 mm within datasets. However, for random subsample sizes equal to a density of ≥15, the variance is greatly reduced to <0.07 mm for 95% of vials (Figure 7). On this basis, only vials with >15 measured pupae were included for analysis or presentation throughout this study.
In measuring panels of RILs, it is useful, for practical reasons, to minimize the number of replicate vials measured for each RIL to estimate an RIL mean with an acceptable degree of variance. Using eight RILs and the two control lines, all of which were measured 12 times or more, it is possible to explore the relationship between RIL means and the number of replicate vial measurements used. Figure 8 indicates that six replicated measurements generally result in 95% of RIL estimates being <0.1 mm different from that based on larger numbers of replicate vial measurements. The mean number of replicate vial measurements per RIL in this study was 6.4 ± 2.1 SD (5.8% = the minimum four replicates, 18.0% = five replicates, 61.2% = six replicates, and 15% more than six replicates).
Estimates of the additive genetic impact on the variance of the trait can be gained from the slope of regressing the parental midpoint [(length of father + length of mother)/2] against the length of the progeny (Galton 1886). Figure 9 indicates that, despite the eight-way (67 crosses) and four-way datasets (363 crosses) having no overlap in biological material, and having quite distinct sampling properties, they both result in remarkably similar estimates of h2, when representing all offspring of a cross as a mean. If all offspring are represented individually, then the estimate of heritability is largely unchanged for both datasets (Table 2). This robustness in the estimates is shared with human height (Table 2 and Hanley 2004). Likewise, regressions that use only the father or mother measurements, rather than their midpoint, confirm that paternal and maternal effects are of equal magnitude for both pupal length and human height (see Figure S3).
Estimates of all potentially genetic (H2) impacts on the variance of the trait can be obtained by estimating the proportion of the total variance in mean vial length measurements related to RIL stock. Table 2 summarizes the results with both the eight-way (195 RILs) and four-way (81 RILs) datasets generating very similar estimates of H2 of 0.58 and 0.61, respectively. The significance of ANOVA tests reflects the strong genetic signal eight-way P = 2.7 × 10−140 (F = 10.86, Mean Square = 0.093, d.f. = 194, sum of squares = 18.3) four-way P = 8.8 × 10−50 (F = 9.026, Mean Square = 0.087, d.f. = 80, sum of squares = 6.9).
Using 195 RILs from the eight-way dataset, and the DSPR tools, a genome scan for genomic regions associated with mean RIL pupal length was conducted (unweighted mean of replicate vial means). The density of markers used in the analysis is one SNP per 10 kb across almost the entire reference genome (excluding the mtDNA and Y chromosome). No SNPs of significance were identified at α = 0.05; this was also the case if density corrected RIL means were used (Figure 10).
While (Visscher et al. 2010) stated that “One could argue that height in humans is the equivalent of bristle number in Drosophila, in terms of its role as a model phenotype.” we propose that pupal length is as good, if not a better, analog, based on its biological properties described in detail for the first time here. This is in addition to the capacity to automate phenotyping using a reliable low cost system. Heritability is estimated using two distinct measures, broad sense (H2) and narrow sense (h2), for both of two biologically independent datasets. This allows the consistency of heritability estimates to be assessed between two datasets, which likely span most of the range of the trait within D. melanogaster. Furthermore, the contrast between h2 (additive genetic effects only) and H2 (additive + nonadditive effects) permits the magnitude of nonadditive effects to be estimated. The properties of pupal length established in this manner are potentially salient to efforts to develop resource efficient means to dissect genetically complex traits, and similar to those reported for human height (Table 3). The key parameter being the high and consistent heritability estimates, which, in part, reflect the reliability of automated measurements where the estimated precision of measurements of 0.04 mm is small relative to the range of the trait as a species (1.1 mm), or within datasets (0.8–0.9 mm). The reliability of pupal length measurements also likely contributes to the normality of the distributions observed at every hierarchical level in the datasets (e.g., Figure 1).
The ease of phenotyping large numbers of individuals at a developmental stage where they are obligatorily stationary for 2–3 d has enabled robust insight into the heritability of pupal length at 24°. The very similar estimates (Table 2) of heritability for two independent datasets provides increased confidence that this trait represents an excellent prospect with which to attempt to identify the genes underlying this complex trait. Furthermore, the similarity between the observed H2 estimates and those expected based on a purely additive model of inheritance (see last row in Table 2) indicates that epistatic, dominance, and paternal or maternal effects are relatively modest (Mackay 2013). The observed similarity in the two h2 estimates (and the associated correlation R2) between the four-way and eight-way datasets (Figure 9) could also be argued to reflect a modest role for dominance. This is because, while the parents and offspring of the four-way crosses have similar levels of heterozygosity, the eight-way parents are inbred RILs while their offspring are heterozygous. Under some forms of dominance effects, both the slope and the correlation of the regression could be reduced in the latter case, which is not the case for pupal length. With respects to maternal and paternal effects, the data presented here (Figure S3) provide evidence that any nonadditive paternal or maternal effects are small, as the size of the effect mothers and fathers exert on their offspring is symmetrical [which is also a property of human height (Hanley 2004)].
It has been detailed above that the impact of density within vials on pupal length is relatively modest (mostly <0.1 mm, Figure 5 and Figure 6), and can be conveniently controlled for. While other environmental variables were not systematically explored, limited data collected for the two control lines indicates that between 18° and 24° pupal length changes by 0.2–0.4 mm (Figure S4), with pupal length being greater at cooler temperatures. The correlation between adult body weight and pupal length was also briefly examined and found to be R2 = 0.6 for both males and females (see Figure S5). Any correlation between adult body length and pupal length was not examined, though it is potentially noteworthy that individuals possessing the Tb1 mutation resulting in a strikingly short tubby pupa are not readily distinguished from wildtype as adults based on their body length. Given the large numbers of genes impacting human height (Table 1), it is unsurprising that it can be correlated with disparate traits of particular interest (e.g., cancer risk, cardiovascular disease and longevity [Nelson et al. 2015; NCD Risk Factor Collaboration (NCD-RisC) 2016)]. Future examination of traits correlated with pupal length (including their reaction norms) may prove informative with respect to their genetic architecture and that of pupal length.
Currently, the automated system does not attempt to distinguish male pupae from female pupae, but it is likely that it could be extended to generate probabilistic sex assignments based on the fact that male pupae are on average ≈8% smaller than females (data not shown). When human height data are corrected for sexual dimorphism [also an 8% average difference between sexes (Galton 1886; Hanley 2004)], there is a corresponding increase of the heritability estimate by 9% (see Table 1 in Hanley (2004)). This implies that analyzing sexed pupal length data will generate heritability estimates that exceed the values reported here for unsexed pupae (Table 2).
The observation that an average 20% ± 11.7 SD of pupae in a vial are not measured by the automated system (Figure 4) is generally not an issue for most experiments (even for select and resequence approaches), as it is often necessary that only a substantial proportion of individuals are measured, which is sufficient to provide a range of parents for the next generation, and to reduce the variance of mean vial estimates (Figure 7). Furthermore, parameters provided in Figure 7 and Figure 8 will provide future researchers with the capacity to readily design maximally resource-efficient experimental strategies.
The observation that the genome scan using 195 RILs (eight-way dataset) failed to identify any significant loci impacting pupal length should be considered in light of the fact that, with this modest number of RILs, the power to detect SNPs of 10 or 5% effect size is only ≈0.37 and ≈0.08, respectively [based on simulations incorporating these exact RILs, see Figure 9 (King et al. 2012b)]. Consequently, the results presented here should be viewed as only potentially sufficient to indicate that few, if any, loci of large effect of size are likely to exist for this trait among the RILs used. It is of course conceivable that phenotyping more of the >1600 RILs currently available would identify significant loci (King et al. 2012a; Huang et al. 2014), and there are several in Figure 10 that are close to the 0.05 significance threshold. Alternatively, an expanding variety of other approaches could be applied e.g., select and resequence (Turner et al. 2011; Nuzhdin and Turner 2013; Kofler and Schlötterer 2014), or comparisons between parallel selected lines (Chan et al. 2012). While this needs to be further explored in the future, it is clear that the automated phenotyping system will be a key to designing mapping strategies that should allow the identification of many low effect size genes for this trait.
The apparatus used to photograph pupae can be rapidly assembled for ~500 € (including a camera), or may already be present in many laboratories as geldoc systems. Furthermore the necessary software is open source and free, and runs on any standard desktop PC or MAC. The capacity of the automated system to provide a large number of phenotyped individuals provides increased power to examine the genetic architecture of traits by most approaches. This is likely to prove key to identifying genes or alleles influencing mean pupal length and its variance (Wood et al. 2014). This is in addition to potentially facilitating exploring the poorly understood genetic basis of sexual dimorphism (Rawlik et al. 2016), and loci which impact the variance of traits [rather than their central values (Ayroles et al. 2015)]. In addition, the capacity to select from large numbers of individuals from which to establish future generations has the potential to enhance select and resequence approaches (Turner et al. 2011; Nuzhdin and Turner 2013; Kofler and Schlötterer 2014). Furthermore, the large numbers of powerful approaches based on single pair matings developed by animal and plant breeders (Gianola and Rosa 2015) may also become amenable to examine complex traits in Drosophila through the use of this simple, low-cost, system. As with human height, over its typical range the genetic architecture of pupal length is of limited practical interest. However, for the last 130 yr, the former continues to provide key insights into the methods through which complex traits can be best understood, this is in part due to the ease and reliability of human height measurement. The high heritability of pupal length, and the capacity to easily automate phenotyping, combined with the small and well-described genome of D. melanogaster could make pupal length a similarly valuable model trait.
Supplemental material is available online at www.g3journal.org/lookup/suppl/doi:10.1534/g3.117.039883/-/DC1.
We thank the following individuals: Stuart Macdonald for his help with the Drosophila Synthetic Population Resource (DSPR) stocks and tools and for maintaining this valuable common resource, Masayoshi Watada for his invaluable advice and access to his personal fly stocks, Predrag Kalajdzic for karyotyping the founder lines of the four-way cross, Oliver Hirsch for help with the development of the semi-transparent barcode stickers, Carolina Wählby for help with extending the use of the “untangle worms” module of Cellprofiler, and Anita Moeller for her excellent technical help and advice with conducting this experiment.
Communicating editor: B. Oliver