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Genome Announc. 2017 March; 5(13): e00083-17.
Published online 2017 March 30. doi:  10.1128/genomeA.00083-17
PMCID: PMC5374233

Draft Genome Sequence of Corynebacterium pseudotuberculosis Strain PA06 Isolated from a Subauricular Abscess in an Ovine Host


We report here the draft genome sequence of Corynebacterium pseudotuberculosis PA06, isolated from a subauricular abscess in an ovine host. C. pseudotuberculosis is a worldwide pathogen of small and large ruminants. The genome comprises 2,320,074 bp, with a G+C content of 52.2%, 2,195 coding sequences, 48 tRNAs, and three rRNAs.


Corynebacterium pseudotuberculosis is a Gram-positive, nonmotile, non-spore-forming, pleomorfic, intracellular, and aerobic-facultative bacterium (1). This bacterium is the etiological agent of caseous lymphadenitis (CLA) disease in small ruminants. Besides its veterinary interest, this pathogen is a zoonotic agent and may be associated with many diseases distributed worldwide (2). The pathogenic effects of this agent have been observed in different hosts, including ulcerative lymphangitis in horses and ulcerative dermatitis in cattle. Recently, C. pseudotuberculosis has also been reported in pigs (3), and, by molecular genetic evidence, it was demonstrated that a veterinary student had pneumonia caused by this pathogen (4).

The primary pathology of this bacterium, caseous lymphadenitis, is a contagious disease that is characterized by the presence of abscesses in the lymph nodes and internal host organs (2). Considering the difficulties in identifying the disease’s early stages and the presence of antigens produced by infectious agents (5), caseous lymphadenitis presents itself as a difficult disease to eradicate. Thus, it is responsible for large economic losses by reducing wool quality, decreasing milk production, lowering weight, and causing death and damage to carcasses (2).

C. pseudotuberculosis PA06 was obtained from a caseous secretion from a punctured lymph node in the subauricular region of a sheep host (Santa Inês breed) located on a farm in Pará State, Brazil. The draft genome was sequenced using the Ion Torrent Personal Genome Machine (PGM) platform (Thermo Fisher) with a fragment library. The genome assembly was performed by SPAdes version 3.9.0 (6) with k-mers of 31, 33, 35, 61, 63, and 65, resulting in 20 contigs. Additionally, MIRA version 4.0 (7) was used with default parameters to reduce the assembly to seven contigs. MAUVE version 2.4 (8) was used to generate the scaffold that was automatically annotated with RAST version 2.0 (9). The genome has 2,195 coding sequences, three rRNAs, 48 tRNAs, and a G+C content of 52.2%.

Accession number(s).

This whole-genome shotgun project has been deposited in GenBank under the accession number CP019289.


This work was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Council for Scientific and Technological Development (CNPq), and the Genome and Proteome Network of the State of Pará (RPGP).


Citation Marques JM, de Moura VAG, Lima ACS, Paixão CTM, Lobato ARF, Alves JTC, Guaraldi ALDM, Folador ARC, Ramos RTJ, Silva A. 2017. Draft genome sequence of Corynebacterium pseudotuberculosis strain PA06 isolated from a subauricular abscess in an ovine host. Genome Announc 5:e00083-17.


1. Dorella FA, Pacheco LG, Oliveira SC, Miyoshi A, Azevedo V 2006. Corynebacterium pseudotuberculosis microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Vet Res 37:201–218. doi:.10.1051/vetres:2005056 [PubMed] [Cross Ref]
2. Arsenault J, Girard C, Dubreuil P, Daignault D, Galarneau JR, Boisclair J, Simard C, Bélanger D 2003. Prevalence of and carcass condemnation from maedi–visna, paratuberculosis and caseous lymphadenitis in culled sheep from Quebec, Canada. Prev Vet Med 59:67–81. doi:.10.1016/S0167-5877(03)00060-6 [PubMed] [Cross Ref]
3. Oliveira M, Barroco C, Mottola C, Santos R, Lemsaddek A, Tavares L, Semedo-Lemsaddek T 2014. First report of Corynebacterium pseudotuberculosis from caseous lymphadenitis lesions in Black Alentejano pig (Sus scrofa domesticus). BMC Vet Res 10:218. doi:.10.1186/s12917-014-0218-3 [PMC free article] [PubMed] [Cross Ref]
4. Heggelund L, Gaustad P, Håvelsrud OE, Blom J, Borgen L, Sundset A, Sørum H, Froland SS 2015. Corynebacterium pseudotuberculosis pneumonia in a veterinary student infected during laboratory work. Open Forum Infect Dis 2:ofv053. doi:.10.1093/ofid/ofv053 [PMC free article] [PubMed] [Cross Ref]
5. McKean SC, Davies JK, Moore RJ 2007. Expression of phospholipase D, the major virulence factor of Corynebacterium pseudotuberculosis, is regulated by multiple environmental factors and plays a role in macrophage death. Microbiology 153:2203–2211. doi:.10.1099/mic.0.2007/005926-0 [PubMed] [Cross Ref]
6. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi:.10.1089/cmb.2012.0021 [PMC free article] [PubMed] [Cross Ref]
7. Chevreux B, Pfisterer T, Drescher B, Driesel AJ, Müller WE, Wetter T, Suhai S 2004. Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs. Genome Res 14:1147–1159. doi:.10.1101/gr.1917404 [PubMed] [Cross Ref]
8. Darling AC, Mau B, Blattner FR, Perna NT 2004. Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 14:1394–1403. doi:.10.1101/gr.2289704 [PubMed] [Cross Ref]
9. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O 2008. The RAST server: rapid annotations using subsystems technology. BMC Genomics 9:75. doi:.10.1186/1471-2164-9-75 [PMC free article] [PubMed] [Cross Ref]

Articles from Genome Announcements are provided here courtesy of American Society for Microbiology (ASM)