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Serotonin receptors are targets of drug therapies for a variety of neuropsychiatric and neurodegenerative disorders. Cocaine inhibits the re-uptake of serotonin (5-HT), dopamine, and noradrenaline while caffeine blocks adenosine receptors and opens ryanodine receptors in the endoplasmic reticulum. We studied how 5-HT and adenosine affected spontaneous GABAergic transmission from thalamic reticular nucleus (TRN). We combined whole-cell patch clamp recordings of miniature inhibitory post-synaptic currents (mIPSCs) in ventrobasal (VB) thalamic neurons during local (puff) application of 5-HT in wild type (WT) or knockout mice lacking 5-HT2A receptors (5-HT2A −/−). Inhibition of mIPSCs frequency by low (10 μM) and high (100 μM) 5-HT concentrations was observed in VB neurons from 5-HT2A−/− mice. In WT mice, only 100 μM 5-HT significantly reduced mIPSCs frequency. In 5-HT2A−/− mice, NAN-190, a specific 5-HT1A antagonist, prevented the 100 μM 5-HT inhibition while blocking H-currents that prolonged inhibition during post-puff periods. The inhibitory effects of 100 μM 5-HT were enhanced in cocaine binge-treated 5-HT2A −/−. Caffeine binge treatment did not affect 5-HT-mediated inhibition. Our findings suggest that both 5-HT1A and 5-HT2A receptors are present in presynaptic TRN terminals. Serotonergic-mediated inhibition of GABA release could underlie aberrant thalamocortical physiology described after repetitive consumption of cocaine.
Serotonin receptors and their associated intracellular pathways are conserved through evolution and have been described as targets of drug therapies for a variety of neuropsychiatric and neurodegenerative disorders (Nichols & Nichols, 2008), and alterations in 5-HT receptor levels have been demonstrated in human patients suffering from several psychiatric disorders (Lopez-Figueroa et al., 2004). Some of these neuropsychiatric disorders involve dysregulation (i.e., altered inhibitory processing) of cortical afferents from the ventrobasal thalamus (VB), which is normally regulated by inhibitory input from the thalamic reticular nucleus (TRN) (Llinas et al., 2002).
Hyperpolarization-activated cyclic nucleotide-gated H-currents have been described to be activated in response to membrane hyperpolarization and contribute to the pacemaker depolarization that generates rhythmic activity of thalamcortical neurons (McCormick & Pape, 1990a). In VB neurons, 5-HT depolarized the membrane potential (Varela & Sherman, 2009) after changing the voltage-dependence of H-currents (Lee & McCormick, 1996) while increasing their amplitude (McCormick & Pape, 1990b). Serotonin can also depolarize (Sanchez-Vives et al., 1996; Monckton & McCormick, 2002) without affecting high-frequency (40Hz) action potential firing of GABAergic TRN neurons (Pinault & Deschenes, 1992). However, other authors showed that bath-applied 5-HT hyperpolarized VB neurons (Monckton & McCormick, 2002). TRN neurons express both 5-HT1A and 5-HT2A receptors in their somas and dendrites (Cornea-Hébert et al., 1999; Rodriguez et al., 2011), but the role of serotonergic receptors at presynaptic GABAergic terminals of TRN are still unclear.
Caffeine (a well-known antagonist of IP3 receptors and an agonist of ryanodine receptors; McPherson et al., 1991) induces Ca2+ release from neuronal ryanodine intracellular stores (Garaschuk et al., 1997; Rankovic et al., 2010), and blocks adenosine receptors (Fredholm, 1995; Fredholm et al., 1999). Caffeine-induced Ca2+ release from the endoplasmic reticulum is known to trigger spontaneous GABA release in retinal amacrine cells (Warrier et al., 2005), as well as spontaneous Glutamate release in rat barrel cortex (Simkus & Stricker, 2002). Adenosine inhibits thalamocortical glutamate efferents reportedly activating presynaptic A1-type receptors (Fontanez & Porter, 2006), and has anti-oscillatory effects by blocking GABAergic transmission between TRN and VB neurons (Ulrich & Huguenard, 1995). The activation of presynaptic A1-receptors strongly suppressed 5-HT2A-mediated increases in spontaneous excitatory minis in cortical layer V pyramidal neurons (Stutzmann et al., 2001). However, little is known about the role of serotonergic 5-HT2A receptors on GABAergic transmission at a thalamic level.
In a previous study (Goitia et al., 2013), our group showed that cocaine binge administration led to considerable disinhibition of thalamic GABAergic transmission, while methylphenidate (MPH) did not induced such alterations. Given that MPH has no effect on serotonin transporters (Glowinski & Axelrod, 1966; Ross & Renyi, 1969; Ritz et al., 1987; Wise & Bozarth, 1987; Pan et al., 1994; Kuczenski & Segal, 1997; Segal & Kuczenski, 1999; Howes et al., 2000), we hypothesized that differences observed between these psychostimulants could be due to cocaine-dependent prolonged activation of serotonergic receptors expressed at the TRN nucleus level (Rodriguez et al., 2011).
Here we studied the presynaptic role of 5-HT on spontaneous GABAergic release from the TRN to the VB nucleus. We used focal (puff) application of 5-HT in slices from mice (WT or 5-HT2A −/−) treated with either cocaine or caffeine binge. Our results described for the first time a strong inhibition of miniature inhibitory post-synaptic current (mIPSC) frequency by 5-HT puff application onto VB neurons from WT or 5-HT2A −/− mice. Further characterization of local 5-HT effects suggests that cocaine-induced effects on thalamic GABAergic transmission are mediated by enhancing presynaptic 5-HT1A inhibitory action on the terminals of TRN neurons.
Our results suggest that inhibition of GABA release by 5-HT1A receptors is counteracted by the activation of 5-HT2A receptors. 5-HT1A receptors, through the activation of Gi/o protein, would inhibit adenylate cyclase while increasing the probability of the opening of G-protein-activated inwardly rectifying K+ channels (GIRK), which would hyperpolarize the membrane potential, facilitating the opening of H-channels (Millan et al., 2008; Luscher et al., 1997). However, 5-HT2A-mediated activation of PLC and IP3, increasing intracellular [Ca2+], would facilitate GABA release (Millan et al., 2008). In addition, caffeine treatment would exert a blocking effect on A1 receptors (Fredholm, 1995; Fredholm et al., 1999), and remove the down-regulation of adenylate cyclase, partially compensating for the inhibitory effects of 5-HT1A receptors on GABA release.
Results presented here could help understand the role of serotonin receptors in multiple neuropsychiatric and neurodegenerative disorders.
We used male 129Sv/Ev mice (18–23 days old), either WT (from the Central Animal Facility at University of Buenos Aires) or knockout for the 5-HT2A receptor (Weisstaub et al., 2006). Principles of animal care were in accordance with the ARRIVE guidelines and CONICET (2003), and approved by its authorities using OLAW/ARENA directives (NIH, Bethesda, MD, USA).
Cocaine (15 mg/kg) or caffeine (5 mg/kg) were administered using ‘binge-like’ protocols (3 i.p. injections, 1 h apart; Spangler et al., 1993; Urbano et al., 2009; Bisagno et al., 2010; Goitia et al., 2013), and control animals received saline injections equally timed. The binge-like administration pattern has been designed to mimic compulsive human cocaine abuse (Spangler et al., 1993).
Slices were obtained as previously described (Urbano et al., 2009; Bisagno et al., 2010; Goitia et al., 2013), 1 hr after the administration of the binge protocol. Mice were deeply anesthetized with tribromoethanol (250 mg/Kg; i.p.) followed by transcardial perfusion with ice-cold low sodium/antioxidants solution (composition in mM: 200 sucrose, 2.5 KCl, 3 MgSO4, 26 NaHCO3, 1.25 NaH2PO4, 20 D-glucose, 0.4 ascorbic acid, 2 pyruvic acid, 1 kynurenic acid, 1 CaCl2, and aerated with 95% O2/5% CO2, pH 7.4), and then decapitated. Thalamocortical brain slices (300 μm) were obtained gluing both hemispheres onto a vibrotome aluminum stage (Integraslicer 7550 PSDS, Campden Instruments, UK), submerged in a chamber containing chilled low-sodium/high-sucrose solution (composition in mM: 250 sucrose, 2.5 KCl, 3 MgSO4, 0.1 CaCl2, 1.25 NaH2PO4, 0.4 ascorbic acid, 3 myo-inositol, 2 pyruvic acid, 25 D-glucose, and 25 NaHCO3). Slices were cut sequentially and transferred to an incubation chamber at 37°C for 30 min containing a stimulant-free, low Ca2+/high Mg2+ normal ACSF (composition in mM: 125 NaCl, 2.5 KCl, 3 MgSO4, 0.1 CaCl2, 1.25 NaH2PO4, 0.4 ascorbic acid, 3 myo-inositol, 2 pyruvic acid, 25 d-glucose, and 25 NaHCO3 and aerated with 95% O2/5% CO2, pH 7.4; Urbano et al., 2009, Bisagno et al., 2010).
Whole-cell patch clamp recordings were made at room temperature (20–24°C) in normal ACSF with MgCl2 (1 mM) and CaCl2 (2 mM). Patch electrodes were made from borosilicate glass (2–3 MΩ) filled with a voltage-clamp high Cl-, high Cs+/QX314 solution (composition in mM: 110 CsCl, 40 HEPES, 10 TEA-Cl, 12 Na2phosphocreatine, 0.5 EGTA, 2 Mg-ATP, 0.5 Li-GTP, and 1 MgCl2. pH was adjusted to 7.3 with CsOH). To block Na+ currents and avoid postsynaptic action potentials, 10 mM N-(2,6-diethylphenylcarbamoylmethyl) triethylammonium chloride (QX-314) was added to the pipette solution (Urbano et al., 2009, Bisagno et al., 2010). Signals were recorded using a MultiClamp 700 amplifier commanded by pCLAMP 10.0 software (Molecular Devices, CA, USA). Data were filtered at 5 kHz, digitized and stored for off-line analysis. Capacitance and leak-currents were electronically subtracted using a standard pCLAMP P/N subtraction protocol. Spontaneous (non-electrically evoked) mIPSCs were recorded from VB neurons in the presence of tetrodotoxin (TTX, 3 μM), DL-2-Amino-5-phosphonopentanoic acid sodium salt (DL-AP5, 50 μM) and 6-Cyano-7-nitroquinoxaline-2,3-dione disodium (CNQX, 20 μM), and analyzed using Mini Analysis (Synaptosoft, Fort Lee, NJ, USA). Cumulative probability amplitude and inter-event interval curves were fitted to a single exponential equation: y = y0+ a*exp(-b*time); and mIPSC median amplitude and intervals (i.e., frequency−1) were compared across groups.
We used focal, puff application of 5-HT to minimize internalization of its receptors, which has been previously described by other authors using both agonists and antagonists (Gray & Roth, 2001). During puff experiments, 5-HT (10 or 100 μM) was focally applied, either alone or together with 1-(2-Methoxyphenyl)-4-(4-phthalimidobutyl) piperazine hydrobromide (NAN-190, 5-HT1A antagonist) or 4-Ethylphenylamino-1,2-dimethyl-6-methylaminopyrimidinium chloride (ZD-7288, a H-current, Ih inhibitor) through a patch pipette filled up with the same ACSF recording solution and connected to a Picospritzer II (General Valve Corporation, Fairfield, NJ) at ~50μm from the cell being patched. In each recording (2min. 30s long), mIPSC frequency was calculated in 15s time bins. The puff was applied at 1:00 to 1:30 min of recording, allowing us to determine pre-puff (0–1:00), puff (1:00–1:30), and post-puff (1:30–2:30) frequencies. Puff and post-puff frequencies are shown as normalized to the pre-puff frequency from each recorded VB neuron.
To test the effectiveness of puff applications on GABAergic synapses, we applied a VB holding potential between −70 and −90 mV (to enhance mIPSC amplitude by over 2 fold peak-to-peak noise amplitude during quantification), and applied ACSF containing CdCl2 (1mM), a Ca2+ channel blocker. This blocked mIPSCs and calcium currents recorded from postsynaptic VB neurons (Fig. 1A, B, respectively). We also confirmed that the mIPSCs being recorded were GABAergic through the puff application of picrotoxin, a GABA-A receptor inhibitor (Fig. 1C).
InfoStat software (Univ. Nacional de Córdoba, Argentina) was used for statistical comparisons. Statistics were performed using Student’s t-test or ANOVA and Tukey-Kramer or LSD Fisher multiple comparisons post hoc tests when applicable. Differences were considered significant if p < 0.05. Whenever the data did not comply with assumptions of the parametric tests, non-parametric Wilcoxon-Mann-Whitney or Kruskal-Wallis tests were performed followed by paired comparisons. Data is presented as mean ± standard error of the mean.
Cocaine-HCl, Caffeine, DL-AP5, CNQX, 5-HT, picrotoxin, and NAN-190 were purchased from Sigma-Aldrich (Argentina), TTX from Alomone labs. (Israel) and ZD 2788 from Tocris (USA).
We studied the role of 5-HT on the frequency and amplitude of spontaneous GABAergic release from the TRN onto VB neurons. We used focal (puff) application of 5-HT in thalamocortical slices from mice (WT or 5-HT2A −/−) in the presence of DL-AP5 (NMDA receptor antagonist), CNQX (AMPA receptor antagonist), and TTX (voltage-gated sodium channel blocker). The puff pipette was located near the soma of VB neurons that were recorded in voltage-clamp whole-cell configuration, allowing us to study presynaptic terminals (Fig. 1). GABA mIPSC frequency was reduced during puff application of 5-HT compared to the pre-puff period, recovering during post-puff period (Fig. 2A; ACSF vs. 5-HT 100 μM). Although an apparent reduction in GABA mIPSC amplitude was observed during puff application of 5-HT, no significance was observed comparing median amplitudes before and during the puff (6.5%±0.8 reduction in median amplitudes puff vs. pre-puff; n=12 VB neurons; Student’s t-test, p>0.05).
Low (10μM) and high (100μM) 5-HT concentration reduced GABA mIPSC frequency during puff (Fig. 2B, filled bars) in a larger percent in VB neurons from 5-HT2A −/− mice compared to WT (two-way ANOVA, p=0.0048). During the 5-HT 10μM puff, percent inhibition was significant only in VB neurons from 5-HT2A −/− mice (Student’s t-test; p<0.0001). During post-puff (Fig. 2B; dashed bars), there were also differences between WT and 5-HT2A −/− (two-way ANOVA, simple effects; p=0.0006). For 5-HT at 10μM, the percent inhibition during post-puff periods was significantly higher for the 5-HT2A −/− group (Student’s t-test; p=0.0006), while for 5-HT 100μM post-puff percent inhibition was significant only for the WT group (Student’s t-test; p=0.0379).
We then determined which 5-HT receptor mediated the 5-HT puff inhibitory effects on GABA release in slices from 5-HT2A −/− mice. We included the specific 5-HT1A antagonist NAN-190 (100 μM) in both the puff pipette and bath extracellular solutions, and observed no significant reduction in GABAergic mIPSC frequency during high concentration 5-HT (100 μM) puff application in 5-HT2A −/− mice (Fig. 3A, B). Applying the H-current blocker ZD-7288 (10 μM) significantly reduced the inhibitory effects of 5-HT 100 μM puff (Fig. 3A, B; One way-ANOVA; p=0.03 comparing 5-HT 100 μM vs. 5-HT 100 μM+ZD-7288 10 μM in VB neurons from 5-HT2A −/− mice). The post-puff effect of 5-HT 100 μM was not significantly affected by either NAN-190 or ZD-7288 application (One way-ANOVA; p>0.05).
We repeated the same experimental approach using slices from WT and 5-HT2A −/− mice treated with either a cocaine binge (3×15 mg/kg, 1 hour apart; i.p.), or a caffeine binge (3×5 mg/kg, 1 hour apart; i.p.) (Fig. 4). Although percent inhibition was significant during the puff for cells from both cocaine-treated WT and 5-HT2A −/− mice (Student’s t-test: p<0.05), cells from cocaine-binge treated 5-HT2A −/− mice manifested a significantly larger inhibition than WT (Fig. 4, left plot; Student’s t-test, p<0.001, cocaine binge treated WT vs. 5-HT2A −/−). Importantly, cocaine binge induced a greater post-puff inhibition only in 5-HT2A −/− mice (One-way ANOVA: p=0.015; comparing post-puff in 5-HT2A −/− vs. post-puff from cocaine binge treated 5-HT2A −/−).
On the other hand, caffeine binge treated WT and 5-HT2A −/− showed significant inhibition in GABA mIPSC frequency only during 5-HT 100μM puff (Student’s t-test: p<0.05), but not during post-puff periods (Student’s t-test: p>0.05) (Fig. 4, right plot). Caffeine reduced 5-HT-mediated inhibition of GABA mIPSC frequency in 5-HT2A−/− mice (Fig. 4, right plot; One-way ANOVA: p<0.001; comparing puff in 5-HT2A −/− vs. puff from caffeine binge treated 5-HT2A −/−).
Results presented here demonstrate that both 5-HT1A and 5-HT2A receptors are located in the presynaptic terminals of TRN neurons (Fig. 5). According to the canonical intracellular pathways of these receptors (Millan et al., 2008), the inhibitory role of 5-HT1A receptors on synaptic GABA release could be mediated by their inhibition of adenylyl cyclase and the activation of H-currents. Adenosine receptor type 1 was previously described in these neurons (Ulrich & Huguenard, 1995; Dixon et al., 1996), and would also use this intracellular pathway, occluding the activation of 5-HT1A receptors. On the other hand, 5-HT2A receptors would activate phospholipase C (PLC) pathways, increasing intracellular [Ca2+] and its concomitant augmentation of GABA release (Fig. 5).
Here, we used focally applied 5-HT onto reticular afferents and VB neurons. This experimental approach minimized desensitization followed by internalization of 5-HT receptors during minutes to hour-long bath application of agonists/antagonists (Gray & Roth, 2001). Inhibitory effects of 5-HT on GABA mIPSC frequency suggested that 5-HT receptors were acting presynaptically. Combining pharmacological tools with mice lacking 5-HT2A receptors (5-HT2A−/−; Weisstaub et al., 2006), we described inhibitory 5-HT1A receptors at presynaptic terminals from 5-HT2A−/− mice. The lower inhibitory effects of 5-HT 100 μM puff in VB neurons from WT mice suggested that 5-HT2A receptors could counteract the inhibitory actions of 5-HT1A. Only 5-HT1A and 5-HT2A receptors were involved in the modulation of GABA release from presynaptic terminals of TRNs (Fig. 5), since applying the 5-HT1A receptor specific antagonist NAN-190 in slices from 5-HT2A−/− mice yielded no effect of a 5-HT 100 μM puff.
These results are consistent with previous immunohistochemical reports describing the expression of both 5-HT1A and 5-HT2A receptors and 5-HT transporter (SERT)-containing afferent fibers at the somas of TRNs (Rodriguez et al., 2011). At the somatic level of these neurons, it has been reported that bath applied 5-HT depolarized VB cells (Sanchez-Vives et al. 1996; Monckton & McCormick 2002) without affecting high frequency (40Hz) action potential firing (Pinault & Deschenes, 1992).
In other brain structures, 5-HT1A inhibitory and 5-HT2A excitatory effects on synaptic release have also been reported. In rat pyriform cortex, bath applied 5-HT (100 μM) and 4-Iodo-2,5-dimethoxy-α-methylbenzeneethanamine hydrochloride (DOI, a 5-HT2A specific agonist; 10 μM) increased GABAergic mIPSC frequency in pyramidal neurons trough activation of 5-HT2A receptors (Marek & Aghajanian, 1996). In prefrontal cortical slices from rats in the third postnatal week (age range similar to the one used in this study), bath-applied 5-HT initially elicited a depolarization (pharmacologically determined to be mediated by 5HT2A receptors) of layer V pyramidal neurons then gradually shifted to a long duration hyperpolarization period mediated by 5-HT1A receptors (Béïque et al., 2004). 5-HT2 receptor activation has been shown to facilitate GABA release, while 5-HT1 receptors were involved in preventing such activation (Fink & Göthert, 2007). Bath-applied 5-HT inhibited Ca2+ currents of caudal Raphe neurons through the activation of 5-HT1A receptors (Bayliss et al., 1997). Therefore, 5-HT would inhibit action potential afterhyperpolarization, inducing an increase in action potential frequency in these cells. The inhibitory effects of 5-HT1A are mediated by both neuronal hyperpolarization and inhibition of adenylate cyclase (De Vivo & Maayani, 1986).
Presynaptic actions of 5-HT2A receptors described in our study confirm previously accepted paradigms in which only 5-HT1A receptors were considered to be located at presynaptic sites, while 5-HT2A receptors are thought to be localized in postsynaptic structures (Nichols & Nichols, 2008). Nevertheless, understanding the role of 5-HT1A receptors modulating GABAergic transmission is relevant to their role in multiple psychiatric and neurological disorders. In postmortem schizophrenia patients an increase in 5-HT1A receptor density in prefrontal cortex has been reported (Bantick et al., 2001). Activation of 5-HT1A receptors produced antidepressant-like effects in animal models (Lucki, 1991), and in knockout mice lacking 5-HT1A receptors have been previously used as genetic models of anxiety disorder (Toth, 2003).
Our results also described a key role for H-currents on the presynaptic inhibitory effects mediated by a 5-HT puff. Using 5-HT2A−/− animals, we observed significantly lower inhibitory effects during 100 μM 5-HT puff application after blocking H-currents with ZD-7288. Basal activation of H-currents would normally inhibit GABA release, probably by shunting membrane resistance as described in distal-apical dendritic compartments of cortical pyramidal neurons (Berger et al., 2003). Morphological and functional experiments have confirmed the presence of H-channels negatively influencing GABA release from rodent globus pallidus neurons (Boyes et al., 2007). Furthermore, H-currents can be tonically activated in presynaptic terminals, reducing the spontaneous release of mIPSCs while inactivating Ca2+-gated channels (Huang et al., 2011). 5-HT2 receptors reduced H-currents and influenced the number of those channels (Liu et al., 2003).
The inhibitory 5-HT puff effects were altered after cocaine or caffeine binge treatments. We observed that a cocaine binge prolonged 5-HT-mediated inhibition during post-puff periods in 5-HT2A−/− mice, suggesting an enhancement of 5-HT1A-mediated inhibitory effects. Repetitive cocaine administration attenuated the ability of 5-HT to enhance spontaneous excitatory postsynaptic currents in the medial prefrontal cortex through impairment of 5-HT2A coupling to its intracellular pathways (Huang et al., 2009), which would potentiate a 5-HT1A-mediated inhibition like the one presented here. The fact that a cocaine binge did not affect 5-HT puff-mediated inhibition during GABA release from cells in WT mice may be due to internalization/downregulation processes of 5-HT1A receptors, previously described in rats (Perret et al., 1998). Cocaine-induced desensitization of 5-HT1A autoreceptors was observed in Raphe nucleus after chronic fluoxetine treatment (an antagonist of SERT) (Le Poul et al., 2000). 5-HT1A desensitization is known to occur after the prolonged activation of G protein intracellular pathways (Castro et al., 2003; Shi et al., 2007), which eventually can result in the internalization of those receptors (Gray & Roth, 2001).
Results presented here described a novel mechanism showing that GABA release can indeed be modulated by the interaction between 5-HT1A and 5-HT2A receptors, which supports our previous hypothesis underlying differential effects of cocaine and methylphenidate on TRN synaptic terminals (Goitia et al., 2013). Furthermore, the prolonged inhibition of GABA release described here after cocaine binge treatment may desensitize 5-HT1A receptors, allowing 5-HT2A to play a greater role, resulting in higher GABAergic mIPSC frequencies as described by our group (Urbano et al., 2009; Bisagno et al., 2010; Goitia et al., 2013). Homeostatic compensatory mechanisms (Mee et al., 2004; Baines, 2005) of GABAergic transmission would also explain the previously observed prolonged activation of this inhibitory synapse after cocaine binge treatment (Urbano et al., 2009; Bisagno et al., 2010; Goitia et al., 2013). Serotonergic mediated inhibition in mice treated with cocaine may trigger compensation at the somatic TRN level, reducing the expression of inhibitory 5-HT1A receptors. Further experiments are needed to support this hypothesis.
Adenosine type 1 receptors are present in the somatosensory thalamocortical system (Fontanez & Porter, 2006), that exert robust antioscillatory effects by simultaneously decreasing excitatory and inhibitory synaptic transmission (Ulrich & Huguenard, 1995). The present study involved a binge treatment with a low caffeine dose (5 mg/kg). A previous study that measured brain concentration of caffeine after an intraperitoneal administration (20mg/kg) reported that caffeine brain levels reached up to 100μM (Hepper & Davies, 1999). Therefore it might be estimated that our protocol using 3× 5mg/kg of caffeine might have produced caffeine brain levels in the tens of micromolar range. Such caffeine concentration levels have been ascribed to adenosine receptor blockage (Fredholm, 1995; Fredholm et al., 1999), rather than acting through the inhibition of phosphodiesterases (Aoyama et al., 2011), or the opening of IP3 receptors expressed in TRN (Garaschuk et al., 1997; Rankovic et al., 2010). Furthermore, ryanodine receptors expressed in both TRN (Budde et al., 2000) and VB neurons (Coulon et al., 2009) might also be involved in caffeine modulation of GABA release shown in this study.
At the postsynaptic level, adenosine receptors have been described to inhibit H-currents in relay thalamic neurons (Pape, 1992). Also, adenosine and serotonin receptors enhanced leak potassium currents (Pape, 1992; Coulon et al., 2010). Further experiments are still needed to clarify 5-HT and caffeine role of the intrinsic properties of postsynaptic thalamocortical neurons.
In cortical pyramidal neurons, adenosine A1 receptors preferentially affect 5-HT2A-mediated enhancement of spontaneous postsynaptic excitatory synaptic events (Stutzmann et al., 2001), contrary to what we report here. In our hands, caffeine-mediated inhibition of adenosine receptors located in presynaptic TRN terminals prevented the inhibitory effects of 5-HT1A receptors during 100 μM puffs in slices from 5-HT2A−/− mice. Therefore, only in the absence of 5-HT2A receptors was caffeine able to affect 5-HT mediated control of GABA release. One likely mechanism for these results could be due to blockade of adenosine A1 receptors after caffeine treatment (Fredholm, 1995; Fredholm et al., 1999), releasing their down-regulation of adenylate cyclase. An increase in the abundance of available adenylate cyclase might partially compensate for the previously observed inhibitory effects of 5-HT1A receptors on GABA release at 5-HT2A −/− reticular synaptic terminals.
The present work highlights the role of 5-HT in modulating GABA release at VB thalamic nucleus during normal physiological activity. In addition, novel 5-HT-mediated mechanisms described here might help explain the long-lasting, detrimental effects of cocaine and caffeine dysregulation of thalamic GABAergic transmission. Such mechanisms could induce permanent changes in sensory thalamic processing.
The authors thank María Eugenia Martin, Daniela De Luca and Paula Felman for their excellent technical and administrative assistance. Dr. Bisagno has been authorized to study drug abuse substances in animal models by A.N.M.A.T. (National Board of Medicine Food and Medical Technology, Ministerio de Salud, Argentina). The experiments included in this study comply with the current laws of Argentina. Authors have full control of all primary data and agree to allow the journal to review their data if requested. Authors report no financial conflict of interest, or otherwise, related directly or indirectly to this study. This work was supported by grants from FONCYT-Agencia Nacional de Promoción Científica y Tecnológica; BID 1728 OC.AR. PICT-2012-1769 and UBACYT 2014-2017 #20120130101305BA (to Dr. Urbano) and FONCYT-Agencia Nacional de Promoción Científica y Tecnológica; BID 1728 OC.AR. PICT 2012-0924 Argentina (to Dr. Bisagno). In addition, this work was supported NIH award R01 NS020246, and by core facilities of the Center for Translational Neuroscience supported by NIH award P20 GM103425 and P30 GM110702 (to Dr. Garcia-Rill).
Conflicts of interest: NONE