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Differentiation of naïve CD4+ T cells into functionally distinct T helper subsets is crucial for the orchestration of immune responses. Due to extensive heterogeneity and multiple overlapping transcriptional programs in differentiating T cell populations, this process has remained a challenge for systematic dissection in vivo. By using single-cell transcriptomics and computational analysis using a temporal mixtures of Gaussian processes model, termed GPfates, we reconstructed the developmental trajectories of Th1 and Tfh cells during blood-stage Plasmodium infection in mice. By tracking clonality using endogenous TCR sequences, we first demonstrated that Th1/Tfh bifurcation had occurred at both population and single-clone levels. Next, we identified genes whose expression was associated with Th1 or Tfh fates, and demonstrated a T-cell intrinsic role for Galectin-1 in supporting a Th1 differentiation. We also revealed the close molecular relationship between Th1 and IL-10-producing Tr1 cells in this infection. Th1 and Tfh fates emerged from a highly proliferative precursor that upregulated aerobic glycolysis and accelerated cell cycling as cytokine expression began. Dynamic gene expression of chemokine receptors around bifurcation predicted roles for cell-cell in driving Th1/Tfh fates. In particular, we found that precursor Th cells were coached towards a Th1 but not a Tfh fate by inflammatory monocytes. Thus, by integrating genomic and computational approaches, our study has provided two unique resources, a database www.PlasmoTH.org, which facilitates discovery of novel factors controlling Th1/Tfh fate commitment, and more generally, GPfates, a modelling framework for characterizing cell differentiation towards multiple fates.
CD4+ T cells are key instructors of the immune system. They can display extensive phenotypic and functional diversity, by differentiating into a range of T helper (Th) subsets including Th1, Th2, Th17, Tfh, Th22, Treg and Th9 cells, that are distinguished mainly by cytokine and transcription factor expression profiles. Since Th cells can control infections and drive immune-mediated diseases there remains tremendous interest in the molecular mechanisms that mediate their in vivo differentiation.
Malaria, caused by the protozoan parasite Plasmodium, afflicted 212 million humans in 2015 (1). Both Th1 responses (2) and Tfh-dependent antibody responses (3) can independently protect against malaria, and are elicited simultaneously in malaria-infected individuals (4), as well as in mice challenged with rodent-infective strains such as P. chabaudi chabaudi AS (PcAS) (5). However, the molecular relationships between Th1 and Tfh cells remain unclear during Plasmodium infection, and more generally during any immune challenge. A recent study has demonstrated that the unique T cell receptor of a naïve CD4+ T cell imparted a strong preference for either a Th1 or Tfh fate (6). Nevertheless, for many clones both fates could still emerge, implying that other mechanisms, such as internal stochasticity and cell-extrinsic factors also govern fate choices in vivo. Transcription factors including T-bet, Gata3, RORγT and Bcl6 have been reported to drive and stabilize Th fates, leading to their characterisation as “lineage-defining” molecules. This has tended to present Th differentiation as a choice between mutually exclusive linear pathways. However, transient co-expression of these transcription factors, e.g. of Bcl6/T-bet, and Foxp3/RORγT suggests that overlapping intermediate Th states also exist in vivo. Moreover, substantial heterogeneity occurs in the kinetics of CD4+ T cell responses, resulting in a complex mixture of intermediate states during differentiation, which is not easily resolved via assessment of a small number of molecules.
Conventional dendritic cells (cDCs) are the dominant initial source of antigenic signalling to naïve CD4+ T cells in secondary lymphoid tissues, for example in the spleens of Plasmodium-infected mice (7). In other models, it was shown that cDCs made long-lasting stable contacts with naïve CD4+ T cells to initiate priming (8). Once activated, CD4+ T cells regained motility, permitting further cellular interactions. Consistent with this observation, activated CD4+ T cells required further antigenic stimulation to optimize clonal expansion and Th differentiation (9); cDC were considered the most likely candidates, (8, 10), with other cell types remaining less explored. Studies of mice with altered monocytic responses suggested roles for these cells in CD4+ T cell priming, specifically in tissues with few cDCs (11). Other reports employed cDC-deficiency to illustrate that monocytes could activate naive CD4+ T cells (12). However, few in vivo studies have explored roles for monocytes in Th differentiation where cDC responses remain intact.
Here, we used single-cell RNA sequencing (scRNA-seq) to study Plasmodium-specific TCR transgenic CD4+ T (PbTII) cells during blood-stage PcAS infection in mice. We then employed a computational modelling strategy to reconstruct the molecular trajectories of Th1 and Tfh cells. Finally, we investigated cell-cell interactions based on dynamic expression of chemokines and their receptors, and examined roles for inflammatory monocytes in supporting activated CD4+ T cells towards a Th fate.
We used scRNA-seq to elucidate the development and heterogeneity of Th1 and Tfh cells during PcAS infection (Figure 1A, Figure S1). We transferred naïve, proliferative dye-labeled PbTII cells into congenic wild-type mice, and recovered them at days 2, 3, 4, and 7 post-infection (p.i.) by cell sorting those expressing the early activation marker, CD69, or displaying dilution of the proliferative dye (Figure S2). Flow cytometric measurements of the canonical Th1 markers, T-bet (coded by Tbx21) and Interferon-γ, and Tfh markers, CXCR5 and Bcl6, indicated that these subsets emerged in parallel by day 7 p.i. (13, 14) (Figure 1B-D and S3). Notably, markers of Th2, Th17 or Treg subsets were not upregulated by PbTII cells (Figure S4).
Initially, we used principal component analysis (PCA) to explore the overall transcriptomic landscape of the PbTII cells (Figure S5A). The top principal components were strongly associated with the number of detected unique transcripts (reflective of mRNA content and proliferative status (Figure S5B)), and differentiation (Figures S5C and S6-7, Table S1). As expected, the variability related to previously established Th1 and Tfh gene expression signatures became more prominent with time, separating two subpopulations at day 7 (15) (Figure 1E-1F). Taken together, these results suggested a progressive commitment to Th1 and Tfh fates, and indicated that single-cell transcriptomes could be used for estimating both proliferative states and degrees of differentiation of individual cells.
The results from the PCA suggested that variation in PbTII transcriptomes could be used to reconstruct the transcriptional programs that is underlying the Th1 and Tfh differentiation. To more explicitly model the temporal dynamics of this differentiation process, we developed GPfates, a temporal mixture model that builds on the Gaussian Process Latent Variable Model (GPLVM) (16) and Overlapping Mixtures of Gaussian Processes (OMGP) (17). Briefly, this approach is based on first reconstructing the differentiation trajectory from the observed data (“pseudotime”, Figure 2A-B), thereby establishing an order for the cells. While our model uses the sample time as prior information, the inferred orderings did not strictly adhere to the experimental time points (Figure S8). For example, cells from day 4 p.i. were mixed with some of the cells from day 3 and day 7 at either end of the day 4 pseudotime distribution. This result is consistent with the idea that bulk assessments of cells at specific time points fail to account for the heterogeneity and differential kinetics of responses made by single cells. To assess the robustness of the established ordering, we repeated this analysis without supplying the experimental sampling times to the model, finding overall consistent results (Comp. Supp. Figure 8).
In a second step, GPfates uses the inferred temporal orders as input for a non-parametric time series mixture model (OMGP, (17)). This approach revealed two simultaneous trends emerging during pseudotime (Figure 2C-D), which separated from each other, indicating that a developmental bifurcation occurred.
In a third step, GPfates employs a change point model (Section 4.2 of the Computational Supplement), thereby facilitating to annotate pseudotime after bifurcation. The cell fate split appeared to initiate amongst early day 4 p.i. cells (in pseudo time, Figure 2C-D), an inference that was robust when using bootstrapped subsets of cells (Section 6.2 of Computational Supplement).
We found that differentially expressed genes between the identified trajectories were in agreement with known Th1/Tfh signature genes (15) (Figure 3A-B, and S9), strongly suggesting that the fitted mixture components corresponded to cells with Th1 and Tfh phenotypes. Notably, these bifurcation trends could not be identified by other published methods for reconstructing bifurcating single-cell trajectories (18–22) (Comp. Supp. Figure 14). We also successfully applied GPfates to resolve bifurcation events in other published datasets, (Comp. Supp. Figure 11) (23, 24) (Comp. Supp. Figure 12), suggesting that our approach is more generally applicable for studying cellular differentiation using scRNA-seq data.
While the TCR transgenic approach employed in this study minimized the influence of TCR sequence variability on cell fate determination (6), the strain was Rag-sufficient, thus retaining potential for expression of diverse endogenous TCR chains in addition to the transgenic TCR. Sequence analysis of TCR transcripts in single PbTII cells confirmed universal expression of the PbTII Vα2 and Vβ12 chains (Supplementary Tables 2 & 3), as well as highly diverse, though lower levels of expression of endogenous TCRα chains in many cells (Figure S10). Importantly, Rag-sufficient PbTII cells differentiated as effectively as Rag1-/- PbTII cells into both Th1 and Tfh cells (Figure S11), indicating that endogenous TCR sequences had not influenced Th fate bifurcation.
Given the vast combinatorial diversity of the endogenous TCR sequences, we employed these as unique molecular barcodes to identify ancestrally-related PbTII clones. We identified six clones comprising multiple sibling cells. Of these, two consisted of sibling cells that mapped close to the bifurcation point. For the remaining four clones, siblings exhibited highly diverging patterns of differentiation, with three sibling pairs falling at the extremities of the Th1-Tfh phenotype spectrum (Figure 3C). These results demonstrated that Th1/Tfh bifurcation had occurred at both population and at single clone levels in our system, with the progeny of a single cell populating both Th1 and Tfh compartments.
Next, we sought to identify genes whose expression differed between the Th1 and Tfh branches. We derived a bifurcation statistic to estimate the concordance with bifurcation for individual genes (see section 4.2 of the Computational Supplement text for details, Figure 3D). Among the highest-ranking genes, the most common pattern was up-regulation along the Th1 branch (Figure 3D). This suggested that Tfh cells were in fact developmentally closer to the shared progenitor state than Th1 cells, since the Th1 fate involved up-regulation of numerous genes not expressed in either the progenitor or Tfh states.
To validate the robustness of these gene signatures and the timing of the bifurcation, we repeated the infection, and at days 0, 4 and 7 sequenced additional PbTII cells using the Smart-seq2 protocol (Figure 1A & S12A). A non-linear dimensionality reduction indicated that the single cells from both experiments populated similar transcriptional landscapes (Figure S12B) and that the subset-characteristic co-expression patterns of the bifurcating genes identified by GPfates emerged by day 7 (Figure S12C). Notably, the day 7 cells from each mouse could be separated into distinct Th1 and Tfh subpopulations using the top bifurcating genes (Figure S12D). These results indicated that the bifurcation-associated gene expression patterns were reproducible across experiments and sequencing platforms.
The highest-ranking transcription factors for the bifurcation included Tcf7 for the Tfh fate, and Id2 for the Th1 fate (Figure 3D & 3E). Tcf7 is required for T cell development, and has been recently shown to be instrumental for Tfh differentiation (25, 26). It also represented one of the rare genes defined by a decrease in expression when moving towards the Th1 fate. Id2 is an antagonist of Tcf7 and was recently identified as a key driver of Th1 responses (27). As expected, the hallmark Tfh transcription factor Bcl6 was also strongly associated with the Tfh fate. In Th1 cells, many bifurcating genes encoded immune-related receptors (Figure 3D-E), such as Cxcr6 (Figure S13A and S13B), Ifngr1 and S1pr1, which mediate egress from secondary lymphoid organs. This was consistent with the notion that Th1 cells can migrate to peripheral tissues and remain receptive to external signals. In contrast, the only bifurcating chemokine receptor associated with a Tfh fate was Cxcr5, which is important for trafficking into B cell follicles (28).
Many of the bifurcating genes had no known role in Th differentiation. For example, lgals1 (encoding Galectin-1), a molecule generally implicated in cDC (29) and Treg function (30), was unexpectedly upregulated in PbTII cells around bifurcation, and maintained along the Th1, but not the Tfh trajectory (Figure S14A). This observation was confirmed at protein level (Figure S14B). Next, comparison of Th1/Tfh fates in co-transferred WT and lgals1-/- PbTII cells during PcAS infection (Figure S14C) revealed a specific role for Galectin-1 in supporting Th1 but not Tfh fate (Figure S14D). Together, these data illustrate the potential for the GPfates model to enable identification of factors controlling Th1 and Tfh fates. Further examination of bifurcating genes is facilitated by the online database, www.PlasmoTH.org, accompanying this paper (Figure 3F).
Coinciding with Th1/Tfh differentiation, we also noted up-regulation of Il10 particularly in the Th1 branch (Figure S15A). Most of the Il10-expressing cells also expressed Ifng at equal or higher levels as those expressing Ifng alone (Figure S15B-C). These data revealed the development of Tr1 cells, defined as IL-10/IFNγ co-expressing CD4+ T cells. Given that Il10 expression was associated with the Th1 branch, this suggested that Tr1 cells were developmentally related to Th1 cells. Surprisingly, we found that aside from Il10, only two genes, Trib2 and BC017643, were differentially expressed between Il10/Ifng co-expressing Tr1 cells and Ifng-expressing Th1 cells (Figure S15D). Furthermore, a comparison of gene expression frequencies between Tr1 and Th1 cells, revealed a substantial degree of similarity across the genome (Figure S15E). Together these data strongly suggest that Tr1 cells derive directly from Th1 cells during blood-stage Plasmodium infection.
Clonal expansion, increased aerobic glycolysis, and cytokine expression, are hallmarks of Th cell development whose temporal relationships with each other remain to be fully resolved in vivo. We noted that PbTII cells became highly proliferative around bifurcation, as shown by the upregulation of Mki67 (Figure 4A-B and S16A) and other known proliferation marker genes (31) (Figure S16B). This correlated with cell cycle activity, as inferred from the scRNA-seq data using the Cyclone tool, and confirmed by flow cytometric measurements of DNA content and cell size (Figure 4C-D, Figure S16C). On Day 4 p.i., the cells also increased expression of genes associated with aerobic glycolysis but not oxidative phosphorylation (Figure 4F), an indication of increased metabolic requirements being met by glucose metabolism and increased mTORC1 activity. Consistent with this was the observed elevated levels of ribosomal protein S6 phosphorylation on day 4 p.i. (Figure 4E).
By day 4 p.i., PbTII cells had gone through several rounds of cell division with differing kinetics, and with some cells expressing IFNγ. By comparing Ifng-expressing and non-expressing cells on day 4 p.i., we noted that early Ifng-expressing cells cycled faster and expressed aerobic glycolysis genes more highly than non-cytokine expressing counterparts (Figure 4G). Taken together, our data suggest that around bifurcation, PbTII cells exhibited a highly proliferative and metabolically active state, with those cells cycling fastest and exhibiting most glycolytic activity being the first to acquire the capacity to secrete IFNγ.
To elucidate how PbTII cells transitioned from the proliferative precursor state to Th1 and Tfh fates we sought to resolve the hierarchy of gene expression before and during cell fate bifurcation. In addition to genes directly following the bifurcation trend, we reasoned that expression of genes encoding key decision-making molecules might also be likely to be dynamic and peak prior to the bifurcation. To identify these, we selected those genes displaying interesting non-linear trends in their expression patterns over pseudotime by Gaussian Process regression. This was achieved firstly via a D-statistic (model likelihood ratio) where each gene’s expression pattern over pseudotime was tested for variation unexplained by random noise (32). Based on the D-statistic (> 50.0, figure 5C) we identified 2061 dynamic genes (Figure 5A). Secondly, we ordered these genes according to their peak expression time to provide a temporal overview (Figure 5A), and noted that a substantial fraction of them peaked around bifurcation. These included the Th1-driving genes Tbx21, Il2ra, and Il2rb, supporting our initial hypothesis. Moreover, cells around bifurcation also transcribed the highest number of genes compared to those at all other points in pseudotime (Figure 5B).
This model also infers the lengthscale of the dynamic model, namely the degree of fast-acting behaviour over pseudotime (Figure 5C). Using this additional feature, we noted roughly equivalent dynamics for Tbx21, Il2ra and Il2rb. Furthermore, we noted similar dynamics, though with slightly later peak times, for the chemokine receptors, Cxcr5 and Cxcr3. Closer examination of all chemokine receptor genes also revealed peak expression around bifurcation for Ccr4, but not others (Figure S17). Given that Cxcr5 and Cxcr3 have been associated with Tfh and Th1 cells, respectively (10, 33, 34), and since they exhibited similar dynamics, we hypothesized these were competing receptors that directly influenced Th1/Tfh fate (Figure 5D). Indeed, assessment of Cxcr3/Cxcr5 co-expression around bifurcation revealed a substantial portion of cells expressing both receptors (Figure S18). Thus, our examination of gene expression dynamics revealed large numbers of genes being expressed and peaking around bifurcation, including not only those associated with clonal expansion, but also numerous sequentially-expressed transcription factors and receptors with potential to influence Th fate.
Given similar dynamics and peak expression times for Cxcr3 and Cxcr5, and peak expression around bifurcation for Ccr4 (Figure S17), we reasoned that cell-cell interactions via these receptors controlled Th1/Tfh fate. Hence we considered cell-types that could control Th fate, specifically around bifurcation. Since B cells supported a Tfh fate (Figure S19), we hypothesized that co-ordinated action by myeloid cells provided competing signals to support a Th1 fate.
To study this, we examined splenic cDCs and inflammatory monocytes prior to PbTII bifurcation. We sorted CD8α+ and CD11b+ cDCs and Ly6Chi monocytes from naïve and infected mice (Figure S20) and performed scRNA-seq. PCA of cDCs distinguished the two naïve cell types along PC2 (Figure 6A & S21) with an efficiency consistent with recent data (35), and further highlighted a number of expected and previously unknown cDC subset-specific genes (Figure S22). We next compared naïve cDCs with those from infection (Figure 6A & S21), and separated these along PC6 (Figure 6A). Analysis of differential gene expression in cDCs due to infection identified 30 genes, 29 of which were upregulated (Figure 6B & S23), including transcription factors Stat1 and Irf1, and CXCR3-attractant chemokines Cxcl9 and Cxcl10. Notably, gene expression patterns amongst individual cDCs varied according to the gene. For example, Stat1 and Irf1 were expressed by several naïve cDCs, and further upregulated during infection (Figure 6C). This was similar for Cxcl9, which was expressed by CD8α+ cDCs in naive mice, while Cxcl10 was induced only upon infection (Figure 6C). These data suggested interactions between cDCs and uncommitted CXCR3+ PbTII cells, consistent with a recent study (10). Next, PCA of Ly6Chi monocytes from naïve and infected mice distinguished them from each other along PC2 (Figure 6D & S24). Differential gene expression analysis between naïve and infected groups uncovered ~100 genes, both up- and down-regulated during infection (Figure 6E & S25). A high proportion (~40%) of genes upregulated in cDCs were also induced in Ly6Chi monocytes, including Stat1, Irf1, and Cxcl10 (Figure 6E & F), suggesting possible overlapping functionality. In addition, monocytes expressed other chemokines, including Cxcl2, Ccl2 and Ccl3 (Figure 6E & F). Furthermore, specific examination of all immune cellular interaction genes (Figure S26) revealed emerging variable expression of Tnf, Cd40, Pdl1, Ccl4, Ccl5, Cxcl16, Cxcl9 and Cxcl11 in monocytes, thus suggesting complex cell-cell interactions for Ly6Chi monocytes during infection.
Since Cxcl9-11, Ccl2, Ccl3 and Ccl5 signal through Cxcr3 or Ccr4, which were expressed by activated PbTII cells, we next hypothesized that Ly6Chi monocytes, in addition to cDCs (10), interacted with PbTII cells and influenced Th1/Tfh fate. To test this, we first assessed chemokine expression at protein level by Ly6Chi monocytes (Figure 6G). Kinetics of CXCL9 production was similar in cDCs and Ly6Chi monocytes. Next, we employed LysMCre x iDTR mice, in which Ly6Chi monocytes were depleted after PbTII cell activation, but before bifurcation (Figure 6H, Figure S27). We noted a modest reduction in CD68+ splenic macrophages using this approach (Figure S27B). Using this approach, we found that monocytes/macrophages supported a Th1 but not a Tfh fate (Figure 6H). Together, our data support a model in which activated, PbTII cells are supported towards either a Tfh fate by B cells (Figure S19), or a Th1 fate by chemokine-expressing myeloid cells, including Ly6Chi inflammatory monocytes.
By capturing single CD4+ T cell transcriptomes during an experimental malaria infection, and computationally reconstructing the course of events, we have resolved the bifurcation of naïve CD4+ T cells into Th1 and Tfh cells at a molecular level. Importantly, GPfates modelling of scRNA-seq data is not limited to immune cells or single bifurcation events. This model can also be combined with existing computational workflows, including alternative methods to estimate pseudo-temporal dynamics (19, 36) (see section 5.2 of the Computational Supplement). The GPfates approach permits analysis of cellular differentiation towards two fates (Comp. Supp. Figure 11), and in principle, towards multiple fates (Comp. Supp. Figure 12). However, GPfates exhibits some limitations. Most notably, the ability to identify and time bifurcation events is linked to changes in the transcriptome that reflect these cellular decisions. In particular, because scRNA-seq profiles are subject to high levels of noise, this means that changes will only be detectable with some lag time (Computational Supplement). The processed expression data and the GPfates model presented in this study can be accessed at www.plasmoTH.org, where users can visualise their genes of interest.
Our data provide the framework for revealing molecular insights into the early stages of Th cell differentiation, and describe the sequence of transcriptional events before and after the bifurcation of Th1 and Tfh fates. Transcriptomic profiling previously suggested developmental similarities between Tfh and Th1 cells (37). However, highly immunogenic viral or bacterial infections induced CD4+ T cells to segregate into Bcl6+ (Tfh) or Blimp-1+ (Th1) subpopulations within two days (38, 39). In our parasitic model, single CD4+ T cell transcriptomes remained remarkably similar until four days of infection. Although it is difficult to directly compare infection models, we speculate that Plasmodium infection in mice may not drive Th bifurcation as early as observed with highly immunogenic viruses or bacteria, particularly given evidence of immune-suppression (40).
IL-10-producing Tr1 cells can suppress immune responses, which could aid the treatment of immune-mediated disorders (41), or be detrimental for chronic infections (42). Despite this, their relationship to Th1 cells is not clear (43). In our model, Tr1 cells emerged from the Th1 trajectory. This observation, coupled with similar transcriptomes for Th1 and Tr1 cells provides evidence that Tr1 cells are highly related to, and derive directly from Th1 cells in this model. Thus, our modelling of scRNA-seq data revealed molecular relationships between Th1, Tr1 and Tfh cells, and showed that a single naïve CD4+ T cell can simultaneously give rise to more than one cell fate during experimental malaria.
Activated CD4+ T cells may experience different microenvironments within secondary lymphoid tissue. The observation that bifurcation towards Th1 and Tfh fates was preceded by upregulation of chemokine receptors prompted us to investigate possible cell-cell interactions with chemokine-expressing myeloid cells. Previous studies have highlighted the potential for cDCs in lymph nodes to produce Th1-associated chemokines (10). Our study, which focused on the spleen further implicated inflammatory monocytes in Th1 support. However, since our transgenic approach for depleting monocytes also removed a small portion of splenic red pulp macrophages, we cannot fully discount the possibility that they may partly contribute to a Th1 fate. Nevertheless, we propose that splenic monocytes/macrophages influence bifurcation by supporting a Th1 fate during Plasmodium infection. Our studies emphasise that although cDCs are key for initiating CD4+T cell activation in the spleen, other myeloid cells can also promote a Th1 fate in the presence of cDCs. In contrast, given that CXCR5 was the only chemokine receptor significantly associated with bifurcation towards a Tfh fate, cellular interaction with B-cell follicles may be the primary mechanism for supporting a Tfh fate. Our model proposes that activated, uncommitted CD4+ T cells become receptive to competing chemoattractant signals from different zones of the spleen, and suggests intercellular communication as a major driver of bifurcation. However, upstream of these processes, internal stochasticity in uncommitted CD4+ T cells may control the balance of chemokine receptor expression. Future experiments combining our integrated single-cell genomics and computational modelling with in vivo positional and trafficking data may reveal molecular relationships between internal stochasticity, migratory behaviour and Th cell fate.
The goal of this study was to use scRNA-seq to capture the transcriptomes of individual splenic PbTII cells at various time points during the first week of a blood-stage PcAS infection. Multiple mice were used for most time points to test for possible batch effects, with an independent experimental repeat performed on a different scRNA-seq platform. scRNA-seq data was modelled using Gaussian processes, with statistical testing for significance of both genes and cells associated with the Gaussian processes.
Wild-type and transgenic inbred mouse strains were housed, and employed in blood-stage Plasmodium infections, as described in the supplementary materials and methods.
Splenocytes were isolated and assessed by flow cytometry as described in the supplementary materials and methods.
Single-cell capture and processing, as well as quality control analysis of scRNA-seq data was performed as described in the supplementary material and methods.
Statistical analyses were conducted using R, or Python, or GraphPad prism. The types of statistical tests and significance levels are described in respective figure legends.
Using single-cell RNA sequencing and computational modelling of temporal mixtures we resolve the developmental trajectories of Th1 versus Tfh cells during experimental malaria, and identify novel T cell-intrinsic and extrinsic factors that influence fate.
We thank the Wellcome Trust Sanger Institute Sequencing Facility for performing Illumina sequencing, Wellcome Trust Sanger Institute Single-cell Genomics Core Facility for single-cell sample processing and the Wellcome Trust Sanger Institute Research Support Facility for care of the mice used in these studies. We thank QIMR Berghofer Flow Cytometry and Animal Facilities for expert advice and care of wild-type and transgenic mice. We wish to acknowledge Stephan Lorenz, Joanna Cartwright and Tom Metcalf for expert technical assistance. We thank Guy Emerton for constructing the database and the interface for accessing the data. We thank Michel Raymond for his work in defining cytokines and cell-surface receptors. Susanna Ng is acknowledged for assistance with graphic design.
This work was supported by Wellcome Trust (no. WT098051), European Research Council grant ThSWITCH (no. 260507), Australian National Health and Medical Research Council Project grant (number 1028641) and Career Development Fellowship (no. 1028643), University of Queensland, Australian Infectious Disease Research Centre grants and the Lister Institute for Preventative Medicine. KRJ was supported by grants from EMBL Australia and OzEMalaR. FOB was supported by the Lundbeck Foundation. MZ was supported by the Marie Curie ITN grant “Machine Learning for Personalized Medicine” (EU FP7-PEOPLE Project Ref 316861, MLPM2012).
Author contributionsTL and KRJ performed the single-cell RNA-seq experiments. VS developed the GPfates model in collaboration with MZ, NDL, OS and SAT. DFR and WRH generated the PbTII mouse model. KRJ, RM, IS, MSFS, LGF, ASN, UL, FSFG, PTB and CRE performed the mouse experiments. TL, VS, KRJ, LHL and FOB analysed the data and interpreted the results
MJTS performed the TCR clonality analysis. TL, KRJ, RM, OB, AH and SAT designed the experiments. OS, AH and SAT co-supervised the study. TL, VS, KRJ, OS, AH and SAT wrote the manuscript. All authors have read and approved the manuscript.
The authors declare no competing interests.
Data and materials availability
The data presented in this paper is publically available in the ArrayExpress database with accession number E-MTAB-4388.