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Protein Cell. 2017 April; 8(4): 242–254.
Published online 2017 January 20. doi:  10.1007/s13238-017-0369-7
PMCID: PMC5359184

Transcriptional regulators dictate innate lymphoid cell fates

Abstract

Research on innate lymphoid cells (ILC) has recently been a fast paced topic of immunological research. As ILCs are able to produce signature Th cytokine, ILCs have garnered considerable attention and have been described to represent the innate counterpart of the CD4+ T helper (Th) cells. The development and function of ILCs are precisely regulated by a network of crucial transcription factors, which are also involved in the development or differentiation of conventional natural killer (cNK) cells and T cells. In this review, we will summarize the key transcriptional regulators and their functions through each phases of ILC development. With the phase of ILC lineage commitment, we will focus in particular on the roles of the transcription regulators Id2 and GATA-3, which in collaboration with other transcriptional factors, are critically involved in the generation of ILC fate determined progenitors. Once an ILC lineage has been established, several other transcription factors are required for the specification and functional regulation of distinct mature ILC subsets. Thus, a comprehensive understanding of the interactions and regulatory mechanisms mediated by these transcription factors will help us to further understand how ILCs exert their helper-like functions and bridge the innate and adaptive immunity.

KEYWORDS: innate lymphoid cell, transcription factors, GATA-3, Id2

INTRODUCTION

Research on a new population of lymphocytes, innate lymphoid cells (ILCs), has been on the rise during the past few years. Compared to other cells in the innate immune system, ILCs are unique in that they may produce and secrete cytokines that were classically regarded as CD4+ Th cell products (Eberl et al., 2015b). Thus, ILCs are considered to be an important component of the innate immune system and their development and functionality has drawn considerable attention in the field. Innate immunity is an evolutionarily ancient system. A major feature of innate immunity is the antigen non-specificity. Pattern recongnition (PR) is a well-known manner to initiate an innate response. The innate immune system recognizes pathogen-associated or damage-associated molecular patterns (PAMPs or DAMPs) through pattern recognition receptors (PRRs) in a semi-specific manner (Brubaker et al., 2015). Signals transduced downstream of PRRs may promote the production of pro-inflammatory cytokines or chemokines, including IL-1β, TNFα, IFN-β, IL-8, etc.

In contrast, the adaptive immune system has evolved to generate exquisitely specific responses to particular antigens, which eventually leads to immunological memory. CD4+ T cells are one of the key players in the adaptive immune system. Early T cell precursors (ETPs) develop through multiple selection steps, including CD4CD8 double negative (DN), CD4+CD8+ double positive (DP), and CD4+ or CD8+ single positive (SP) in the thymus. Once the T cell precursors have experienced β-selection, positive and negative selection, they eventually become bona fide naïve CD4+ or CD8+ T cells (Germain, 2002). Several crucial transcription factors are involved in regulating and orchestrating this process, including TCF1, TOX, Bcl11b, GATA-3, Th-Pok, and Runx3, etc. (Yui and Rothenberg, 2014). Naïve CD4+ T cells, after migrating out of the thymus to the periphery, will further differentiate into distinct effector cells upon encountering antigen-laden antigen presenting cells. During this process, the signals triggered by TCR, co-stimulatory receptors and cytokine receptors influence the ultimate effector T helper cell fate of the naïve T cell (O’Shea and Paul, 2010). For example, IL-12 drives the differentiation of type 1 T helper (Th1) cells; IL-4 promotes type 2 T helper (Th2) cells; and IL-6 together with TGF-β facilitates the generation of IL-17-producing T helper (Th17) cells. Differentiated Th effectors are capable of expressing their signature effector cytokines—IFN-γ for Th1, IL-4 for Th2, and IL-17 for Th17 cells. The transcription factors that are deterministic for the differentiation and functions of Th cell subsets, are referred to as master transcription factors and include T-bet for Th1, GATA-3 for Th2, and RORγt for Th17 cells. The precise functions of these master lineage regulators during CD4+ T cell activation and Th effector differentiation have been extensively studied using gain or loss of function animal models.

The production of signature effector cytokines had historically been considered a unique feature of CD4+ Th cells in the adaptive immune system, until the discovery of ILC populations. These innate lymphocytes were overlooked possibly due to their lack of expression of any known lineage markers and their enrichment mainly in the non-lymphoid tissues. The first descriptions of a non-T non-B lymphocyte population that produced the Th2 cytokines, IL-5 and IL-13, began the innate lymphoid cell field (Fallon et al., 2006; Moro et al., 2010; Neill et al., 2010; Price et al., 2010). It is now well known that there are several distinct ILC subsets that can express signature cytokines like Th cells (Eberl et al., 2015a). For example, group 2 ILCs (ILC2s) can produce the effector cytokine IL-5 and IL-13 like Th2 cells, group 3 ILCs (ILC3s) can produce IL-22, IL-17a, and IL-17f as Th17/Th22 cells, and group 1 ILCs (ILC1s) can produce IFN-γ and TNF-α like Th1 cells. Interestingly, in addition to their mirrored cytokine repertoire, both CD4+ T cells and ILC subsets also utilize a similar set of transcriptional factors for their development, differentiation and functions (Artis and Spits, 2015; Zhong and Zhu, 2015b; Zook and Kee, 2016).

In addition to their ability to produce signature cytokines, ILCs are interesting in that they are primarily tissue resident lymphocytes. ILC progenitors are developed in the bone marrow, while mature ILCs are mainly enriched in peripheral tissues such as gastrointestinal (GI) tract, lung, liver, and skin. Recent studies from parabiosis experiments have confirmed that the vast majority of ILCs are tissue-resident (Gasteiger et al., 2015). In addition, a few reports have addressed the question of how bone marrow ILC progenitors home to peripheral tissues. For example, ILC2s gain the gut homing receptor CCR9 and Integrin α4β7 during their development in bone marrow, and thus ILC2s directly migrate to and reside in the gut after maturation. On the other hand, the precursors of ILC1 and ILC3s may initially express the homing receptor CCR7, which directs them to lymphoid organs such as the spleen and lymph nodes. Then upon encountering retinoic acid, ILC1 and ILC3 cells may down-regulate CCR7 and up-regulate both CCR9 and Integrin α4β7, which eventually guides these cells to the gut (Kim et al., 2015). However aside from the gut, it is currently unclear how ILCs may migrate to other peripheral tissues. The process of ILC colonization is further complicated by the fact that ILC precursors are also found in periperal tissues (Bando et al., 2015). At the fetal stage, some ILC progenitor cells have been observed to migrate to and reside in the proximal gut, where they are responsible for the generation of Peyer’s patches. Interestingly, these precursors have the capacity to develop into all three ILC subsets and may thus serve as a resident source of ILCs.

Functionally, ILCs are crucially important in providing protection against pathogens in the early stages of an immune response. For example, ILC2s are an important source of IL-5, IL-13, and IL-9 after an infectious challenge with helminth pathogens (Fallon et al., 2006; Neill et al., 2010; Price et al., 2010; Wilhelm et al., 2011). The production of these cytokines is required for the efficient recruitment of eosinophils (Nussbaum et al., 2013). ILC2s also participate in wound healing via secretion of amphiregulin (Monticelli et al., 2011). Additionally, ILC2-derived cytokines may promote the beigeing of adipose tissue and may thus affect adipose tissue homeostasis (Lee et al., 2015). ILC3 cells are RORγt-expression ILCs. A special subset of ILC3s is the LTi cell which is required for the formation of secondary lymphoid structures such as lymph nodes and Peyer’s patches (Finke, 2005). ILC3s are enriched in the GI tract and are associated with inflammatory bowl diseases. ILC3s are the main source of IL-22 in homeostatic conditions and in the early stages of certain inflammatory responses (Cella et al., 2009; Luci et al., 2009; Sanos et al., 2009; Takatori et al., 2009). IL-22 acts on intestinal epithelium to regulate the homeostatic self-renewal of epithelial stem cells (Hanash et al., 2012; Lindemans et al., 2015) and the production of antimicrobial peptides (Dudakov et al., 2015). Additionally, ILC3-derived IL-22 and lymphotoxin are also required for the fucosylation of the gut epithelial cells by stimulating the expression of fucosyltransferase Fut2 (Goto et al., 2014; Pickard et al., 2014). The fucose residues on the surface of the gut epithelium may provide nutrients for the gut microbiota, thus maintaining gut homeostasis. Recent studies have also indicated that ILC3s in other peripheral tissues, including the skin (Pantelyushin et al., 2012), may also play crucial roles in maintaining the local microenvironment. ILC1s have similar features to conventional NK cells, aside from their lack of cytotoxicity. However, ILC1s are more efficient producers of IFN-γ and TNF-α during a type 1 response and ILC1s provide the innate protection against Toxoplasma gondii infection (Klose et al., 2014).

ILCs may directly or indirectly regulate the adaptive immune response. For example, a deficiency in ILC2s may result in a dramatic reduction in Th2 responses (Oliphant et al., 2014). ILC2-derived IL-13 may promote DC migration to the draining lymph node where the DCs can facilitate T cell activation and Th2 differentiation (Halim et al., 2014). IL-13 also licenses DC subsets to secrete the chemokine CCL17, which is required for the recruitment of memory CD4+ T cells (Halim et al., 2016). In addition, ILC2s may express the antigen-presenting molecule MHCII and can crosstalk directly with Th2 cells to augment immune responses (Oliphant et al., 2014). However, a recent report indicates that a normal Th2 response may occur in the absence of ILC2s (Van Dyken et al., 2016), arguing that the ILC2-derived IL-13 and the potential antigen-presenting functions of ILC2s may not be necessary to mount an effective Th2 response. Interestingly, ILC3s may also express MHCII, however ILC3-dependent antigen presentation represses commensal antigen specific CD4+ effector T cells, due to a lack of or low expression of co-stimulatory molecules on ILC3s (Hepworth et al., 2015; Hepworth et al., 2013).

Thus as the field continues to explore the functions of ILCs, new and novel functions of various tissue-resident ILC subsets are being reported. However the development and function of ILCs are, like all cells, subject to transcriptional regulation and control. Therefore in the remainder of this review, we will summarize the transcription factors that have been shown to critically regulate the functions and development of tissue resident ILCs.

TRANSCRIPTIONAL REGULATION OF ILC DEVELOPMENT

To fully understand the function of ILCs, we need first to understand how this lineage is generated. At present, the regulatory mechanisms of innate lymphoid cell development have been quite elusive. Our initial knowledge on the generation of these cells mainly stemmed from the phenotypes of global gene knockout mice. Nevertheless, it is now clear that the transcriptional regulators that are associated with cNK or T cell development are also involved in ILC development, suggesting that ILCs and T cells have a close developmental relationship. Research on the transcriptional regulation of ILC development became feasible once common ILC progenitor cells were identified. Below is a summary of the critical transcription factors that have been identified to be involved in ILC development (Fig. 1).

Figure 1
Critical transcription factors regulate ILC development. After the stage of common lymphoid progenitor (CLP), transcription factors such as TCF1, TOX and Nfil3, together with the Notch signals, promote the generation of an early innate lymphoid progenitor ...

Id2

Studies on ILC development have advanced progressively since the identification of the ILC fate determined progenitors, the common helper-like innate lymphocyte progenitors (ChILPs) (Klose et al., 2014). Id2 is expressed by ILCs and cNK cells and is required for their development (Verykokakis et al., 2014; Yokota et al., 1999). Id2 belongs to the “inhibitor of DNA binding protein” family, which is comprised of Id1-4. Id proteins share a highly conserved helix-loop-helix (HLH) domain and can negatively regulate other HLH domain-containing transcription factors by forming heterodimers with their HLH domain; thus inhibiting their ability to bind to DNA. E-box proteins, including E2A, E2-2, HEB, are one example of a subset of basic HLH transcription factors that are antagonized by Id proteins (Kee, 2009). Id2 is required for all innate lymphocytes development, partially due to their repression of the E-box proteins (Verykokakis et al., 2014). Indeed, an additional deficiency of E2A in Id2 −/− mice rescues a defect in lymphoid tissue inducer (LTi) cell development as well as a defect in the formation of secondary lymphoid structures (Boos et al., 2007). ChILP cells are defined as lineageIL-7R+Flt-3Integrin α4β7 +CD25Id2high progenitor cells that have committed their fates to an ILC lineage (Klose et al., 2014) (Fig. 1). Some transcription regulators associated with the up-regulation of Id2 have also been identified and will be discussed later. However, the precise mechanisms through which Id2 mediates ILC development and the environmental signals that induce Id2 expression in ChILP cells are still elusive.

GATA-3

In addition to Id2, GATA-3 is another transcriptional regulator that is required for the development of all innate lymphocytes aside from cNK cells (Yagi et al., 2014). GATA-3 is well known for its involvement in T lymphocyte development at various stages (Ho et al., 2009). During CD4+ T cell development, GATA-3 needs to be up-regulated to a proper high level in order to induce Th-pok expression and thus to direct CD4+ T cell generation (Wang et al., 2008). Deficiency in GATA-3 at the CD4+CD8+ double positive (DP) stage results in a failure of CD4+ T lineage commitment (Pai et al., 2003), whereas hyperexpression of GATA-3 by a GATA-3 transgene is toxic to the cells (Taghon et al., 2007). Furthermore, overexpression of GATA-3 at the DN stage redirects these cells developing into mast cells (Taghon et al., 2007). Thus, the level of GATA-3 expression during T cell development needs to be carefully controlled. We have previously found that ILCs require GATA-3 for their development. A conditional Gata3 deficiency starting from the hematopoietic stem cell stage mediated by VavCre results in a developmental defect affecting almost all ILCs (Yagi et al., 2014), consistent with another report showing that a Gata3 germline deletion causes the failure of ILC3 development (Serafini et al., 2014). By tracing GATA-3 expression along ILC development, we and others have found that GATA-3 expression is undetectable at the common lymphoid progenitor (CLP) stage, and that CLP development in Gata3 fl/fl VavCre mice seems to be normal. However within the ChILP cells, a subset expresses high GATA-3 levels, suggesting that high levels of GATA-3 expression at this progenitor stage may be required for the development of all ILCs. As GATA-3 is dispensable for cNK cell development but required for the optimal function of mature cNK cells (Samson et al., 2003), it is intriguing to explore whether the GATA-3 expression levels in innate lymphocyte progenitors can determine their lineage fates towards either helper-like ILCs or cytotoxic cNK cells, in a similar manner to the function of GATA-3 in CD4+ and CD8+ T cell lineage commitment.

PLZF

In addition to ChILP cells, another specialized ILC progenitor population has been identified based on the expression of the transcription factor PLZF; namely the lineagePLZF+ common innate lymphoid progenitor (CILP) (Constantinides et al., 2014) (Fig. 1). Further characterization of these cells has indicated that they are IL-7R+/−, Flt-3, Integrin α4β7 +, Id2+, and GATA-3+. Interestingly, PLZF is only transiently expressed at the ILC progenitor stage. Using a fate-mapping mouse strain to trace current and former PLZF-expressing ILC progenitor-derived cells or by transferring PLZF-expressing CILPs, Bendelac and his colleagues have demonstrated that the progenies of PLZF-expressing progenitors include all ILCs but not LTi cells, indicating that LTi cells develop from a distinct progenitor, which is separated from the PLZF-expressing progenitors that give rise to non-LTi ILCs. However, PLZF itself is not absolutely required for ILC development, despite a modest reduction of ILC2 cells in Zbtb16 / mice (PLZF gene) (Constantinides et al., 2014).

Notch

The Notch signaling pathway is highly conserved in most multicellular organisms and it plays critical roles during lymphoid lineage commitment. For example, T and B cell fate in the early stages after CLP is determined by the on and off status of Notch signals (Radtke et al., 2013). Notch signals are also critically involved in ILC development and functional regulation (Lee et al., 2011; Possot et al., 2011; Wong et al., 2012), possibly through their regulation of Gata3 and/or Tcf7 expression, which needs to be studied further. Interestingly, while Notch signals are required for LTi development, sustained Notch signals can block the generation of LTi cells (Chea et al., 2016). Therefore, Notch signaling seems to be dynamically regulated and its functions may be stage specific during ILC development.

Nfil3

Nuclear factor interleukin-3 (Nfil3, also known as E4BP4) has been recently reported to be a critical transcription factor for the development of all ILCs (Seillet et al., 2014; Xu et al., 2015; Yu et al., 2014), and the development of cNK cells (Gascoyne et al., 2009). Nfil3 can be induced by IL-7 signaling in CLPs (Xu et al., 2015). For both ILC and cNK cell development, Nfil3 can efficiently activate Id2 expression, probably explained by its direct binding to the Id2 locus (Xu et al., 2015). However, Nfil3 is not indispensible for Id2 expression in innate lymphocyte progenitors since both cNK cells and ILCs can still develop in the Nfil3 −/− mice, albeit at lower numbers.

TOX

Thymocyte selection-associated HMG box protein (TOX) belongs to another evolutionarily conserved high mobility group (HMG)-box family. TOX is known to be essential for the development of both adaptive T cells and innate cNK cells. TOX is transiently expressed during the β-selection and positive selection of T cell development and may be involved in the transition from CD4lowCD8low to CD4+CD8low (Aliahmad and Kaye, 2008). TOX is also expressed in developing and mature cNK cells (Aliahmad et al., 2010). Nfil3 may bind to the Tox locus and may positively regulate its expression (Yu et al., 2014). Tox deficiency results in a blockage of cNK development at the lineageIL15Rα+NK1.1DX5 progenitor stage, but the blockage is not associated with Id2 expression (Aliahmad et al., 2010). Tox −/− mice have a profound defect in LTi generation, as indicated by the lack of peripheral lymph nodes and a significantly reduced Peyer’s patch frequency and size (Aliahmad et al., 2010). With regard to ILC development, a Tox deficiency results in a dramatic reduction of ChILPs (Seehus et al., 2015). A comparison of gene expression in wild type and Tox −/− ChILPs suggests that a decrease in Notch signals, in concert with their downstream target genes, including Tcf7, Hes1, Gata3, and Bcl11b might be responsible for the developmental defect in ChILPs.

TCF1

T-cell factor 1 (TCF1, encoded by Tcf7) is another member of the HMG box-containing transcription factors. It is also involved in the development of both T cells and ILCs. TCF1 level during T cell development is up-regulated as early as the early T cell progenitor (ETP) stage, and together with its close homolog, lymphoid enhancer binding factor 1 (LEF-1), TCF1 promotes T cell development possibly by acting as a downstream effector of Notch signaling (Steinke et al., 2014; Weber et al., 2011). It has been recently reported that TCF1 is critical for the development of multiple ILC subsets including ILC2s and ILC3s (Mielke et al., 2013a; Yang et al., 2013). TCF1 expression is induced before the ChILP stage. Based on TCF1 expression, an earlier innate lymphocyte progenitor, termed EILP, has been identified (Yang et al., 2015) (Fig. 1). The EILP has the potential to develop into either cNK cells or ILCs, but has lost its capacity to become T or B cells. EILPs, which are different from ChILPs, express very low levels of Id2, but substantial levels of Nfil3 and TOX transcripts. Thus Nfil3 and/or TOX may be responsible for inducing TCF1 expression, and thus the generation of EILPs. Therefore, Nfil3, TOX, and TCF1 may represent a few upstream transcription factors that are required to set up the environment for Id2 up-regulation and thus ChILP generation. Besides inducing Id2, TCF1 may up-regulate GATA-3 expression, presumably through similar GATA-3 regulation mechanisms found during ILC2 development.

Although the above transcription factors are essential for the generation of ILCs, the regulatory network among them in ILC progenitors, and the precise mechanism through which ILCs are developed still need further investigation. Our current knowledge about ILC development is still quite limited. As ILC progenitors are rare populations in the bone marrow, their low frequency makes it very difficult to establish the precise regulatory mechanisms within these cells. The regulation of ILC development is likely to be far more complicated than we have discussed. Additional un-described transcriptional regulators or environment cues may play large roles in governing progenitor cell fates during development. With the development of more advanced technologies, including single cell transcriptomic analyses, it is now possible to profile the whole transcriptomes of rare progenitor populations and to reveal their heterogeneity (Bjorklund et al., 2016; Gury-BenAri et al., 2016; Ishizuka et al., 2016; Yu et al., 2016). Further studies of ILC progenitors are ultimately necessary to further understand the evolution and lineage specification of the lymphoid system in mice and clinical patients.

SPECIFIC REGULATION OF ILC SUBSETS

In addition to the aforementioned transcription factors that affect the development of ILC progenitors, additional transcription factors play a role in the formation of distinct mature ILC subset. These transcription factors are mostly expressed in or after the ChILP stage. However, some of the transcription factors mentioned above are also involved in the maintenance or functional regulation of select ILC subsets. Hereafter we discuss the transcriptional regulators that function in each particular ILC subsets.

ILC2-associated transcription factors

ILC2s are characterized by very high expression levels of the Th2 master regulator GATA-3, which endows ILC2s with the ability to produce the Th2 effector cytokines, IL-5 and IL-13, stimulated by upstream cytokines including IL-33, IL-25, and IL-2 (Hoyler et al., 2012) (Fig. 2). GATA-3 is required not only for the generation of common ILC progenitors, but also for the maintenance and function of ILC2s. Deletion of Gata3 in committed ILC2 cells leads to diminished cytokine production and eventually cell death (Yagi et al., 2014). GATA-3 directly binds to Th2 cytokine loci in ILC2s as in Th2 cells (Zhong et al., 2016). However, the detailed mechanism of how GATA-3 maintains mature ILC2s is still unclear. It is possible that GATA-3 may regulate cell cycle or cell apoptosis related genes in ILC2s.

Figure 2
ILC2-specific transcription factors. GATA-3, which determines ILC2 development, maintenance and effector cytokines IL-5 and IL-13 production, is the master regulator of ILC2s. RORα, Bcl11b, ETS1, TCF1, and Gfi-1 are involved either in ...

Besides the master regulator GATA-3, other transcription factors such as Bcl11b, RORα, TCF1, and Gfi-1, etc. specifically affect ILC2 cell development and/or function although these factors may also be expressed by other ILCs (Fig. 2).

Bcl11b

B-cell CLL/lymphoma 11b (Bcl11b) is another critical transcription factor for T cell development. It promotes the transition from double negative (DN) 2a stage to DN2b stage, after which the T cell lineage fate is fully determined (Ikawa et al., 2010). The role of Bcl11b in ILC development, however, is restricted to the ILC2 subset. A subgroup of ChILP cells has been found to express Bcl11b and they appear to be the progenitors of ILC2s (Walker et al., 2015; Yu et al., 2015; Zhong and Zhu, 2015a). In Bcl11b −/− mice, ILC2 cells fail to develop. Mature ILC2s in various tissues also express high levels of Bcl11b while the immature ILC2s that are found in the bone marrow express negligible levels of Bcl11b. Bcl11b deletion in mature ILC2 cells does not affect cell survival but results in a loss of ILC2 identity (Califano et al., 2015). Some Bcl11b-deleted ILC2 cells up-regulate RORγt expression and may therefore acquire some ILC3 features. The conversion of ILC2s to ILC3s may be attributable to the regulation of Gfi-1, RORα, and AhR by Bcl11b in ILC2s (Fig. 2). Besides ILC2s, some NKp46+ ILC3 and ILC1 cells also express Bcl11b, however the function of Bcl11b in non-ILC2s is still elusive.

RORα

RORα is expressed in the progenitors as early as at the ChILP stage, however it is specifically required for the generation of ILC2s (Halim et al., 2012; Wong et al., 2012). The underlying mechanism of RORα-mediated ILC2 development is still unclear. RORα is continuously expressed in all mature ILC subsets. Although it is not clear whether RORα has any function in mature ILC1 or ILC2 cells, a recent report indicates that RORα may regulate ILC3 cells (Lo et al., 2016).

TCF1

Although TCF1 is expressed before Id2 during ILC development, its expression is maintained even after the ChILP stage, which is consistent with its critical function in the generation of ILC2 cells through both GATA-3-dependent and -independent mechanisms. The overexpression of TCF1 partially bypasses Notch signaling, which is required for the development of ILC2s, indicating that TCF1 mediates Notch-dependent ILC2 development (Yang et al., 2013). TCF1 is also required for the proper generation of NKp46+ ILC3s (Mielke et al., 2013a).

Gfi-1

Gfi-1 has an important regulatory function in both Th2 cells (Zhu et al., 2002; Zhu et al., 2006) and ILC2s. It has been reported that, in ILC2 cells, Gfi-1 regulates the expression of Gata3 and Il1rl1 (the gene encoding the IL-33 receptor) (Spooner et al., 2013) (Fig. 2). Loss of Gfi-1 in ILC2s results in reduced GATA-3 expression but enhances RORγt expression. Such dys-regulation leads to the co-expression of ILC2 effector cytokine IL-13 and the ILC3 effector cytokine IL-17.

ETS1

ETS1 is a transcription factor that has been recently identified to be particularly important for ILC2 development and function (Zook et al., 2016). Ets1 deficient mice show a substantial reduction of ILC2s in the bone marrow and lymph nodes. However, the total cell number of ILC2s in the lung of Ets1 deficient mice is comparable to that of Ets1 sufficient mice. Together with other reports (Saenz et al., 2013; Spooner et al., 2013), these results suggest that ILC2s in different tissues might develop from separate progenitors that have distinct developmental requirements—an important question which requires further investigation. Similar to its function in cNK cells, ETS1 regulates the fitness of ILC2s and the common ILC progenitors mainly by promoting optimal expression of Id2. In mature ILC2s, ETS1 regulates ILC2 cell proliferation and the effector cytokine IL-5 and IL-13 production in response to IL-33 or IL-25 stimulation (Fig. 2).

ILC3-associated transcription factors

Like Th17 cells, RORγt-expressing ILC3s are similarly not a homogeneous population. They can be divided into at least two lineages, an LTi lineage and a natural cytotoxic receptor (NCR)-expressing ILC3 lineage (Klose et al., 2013). RORγt, as the master regulator for ILC3s, is required for the development and the maintenance of both lineages. However, the development of NCR+ ILC3s requires additional regulators such as T-bet and GATA-3. IL-23 and IL-1β receptors expressed in both ILC3 lineages, and can transduce the upstream cytokine signals for the production of ILC3 cytokines IL-22, IL17a, and IL-17f (Fig. 3).

Figure 3
ILC3-specific transcription factors. RORγt acts as a master regulator for all ILC3 cells, including the CCR6+ LTi/LTi-like cells and the NKp46+ ILC3s. RORγt is also responsible for the production of ILC3 effector cytokines, such as IL-22, ...

GATA-3 and T-bet

Data from our ILC3-specific conditional Gata3 knockout mouse indicates that GATA-3 acts upstream of T-bet to direct NCR+ ILC3 development from a CCR6T-bet precursor (Zhong et al., 2016). GATA-3 inhibits RORγt expression by directly binding to the Rorc locus. Therefore, GATA-3 regulates the balance between RORγt and T-bet, which is critical for the development of NCR+ ILC3s. A two-fold increase or decrease in RORγt expression may dramatically change the outcome in the development of T-bet-expressing ILC3s. Once T-bet expression reaches a sufficient level, it acts as a repressor of RORγt and further promotes the generation of NCR+ ILC3. Besides their critical role during the development in NCR+ ILC3, both GATA-3 and T-bet regulate the production of ILC3 effector cytokine IL-22 (Sciume et al., 2012; Zhong et al., 2016).

AhR

Aryl hydrocarbon receptor (AhR), the master regulator in xenobiotic metabolism, has been reported to influence several other cellular functions including the development of immune cells (Zhou, 2016). The AhR protein contains a bHLH DNA binding domain and a Per-Arnt-Sim (PAS) domain for agonist binding. Before binding its agonist, AhR forms an inactive complex with heat shock protein 90 (Hsp90), aryl hydrocarbon receptor interacting protein (AIP) and p23 in the cytosol. After binding to its agonists, a conformational change in AhR releases itself from the complex and thus enables its translocation to the nucleus. After AhR dimerizes with another bHLH-PAS protein aryl hydrocarbon receptor nuclear translocator (ARNT), AhR initiates the transcription of its downstream target genes such as Il22 and Kit in ILC3s (Fig. 3). AhR is a regulator for both LTi and NCR+ ILC3s. AhR −/− mice have a defect in the development of LTi cells and thus lack tertiary lymphoid structure cryptopatches (CPs) and isolated lymphoid follicles (ILFs) in the gut (Kiss et al., 2011). NCR+ ILC3 cells are also reduced in the AhR −/− mice (Qiu et al., 2012). One of the AhR regulated genes in ILC3s is c-kit, which is expressed in most of the ILC3 cells (Kiss et al., 2011). The frequency of AhR −/− ILC3s is still normal at early time point after birth, but the AhR −/− ILC3s are not able to survive consistent with reduced c-kit expression in such cells.

Runx3

Runx3 is a master regulator critical for CD8+ T cell development (Woolf et al., 2003). However Runx family members, including Runx1 and Runx3, and their common obligatory partner Cbfβ, are associated with LTi cell generation (Ebihara et al., 2015). A deficiency of these genes results in a defect in the generation of secondary lymphoid structures (Tachibana et al., 2011). However, since the development of CLP is also affected by Runx deletion, it is still not clear whether Runx proteins are specifically required for the development of ILCs. Based on a reporter expression study, Runx3 was found to be specifically expressed in ILC3s and ILC1s, but not in ILC2s (Ebihara et al., 2015). Early deletion of Runx3 leads to a blockage of ILC3 development at the RORγt stage. Deletion of the Runx common obligatory partner Cbfb in mature NKp46+ ILC3s (Cbfb fl/flNKp46-Cre) also leads to a lack of NKp46+ ILC3 and ILC1 cells. Furthermore, Runx3 regulates RORγt and subsequent AhR expression in all ILC3 cells (Fig. 3).

Retinoic acid signals

Retinoic acid (RA) is the active metabolite of Vitamin A, which activates the nuclear receptors retinoic acid receptor (RAR) or retinoid X receptor (RXR). RAR or RXR hetero-dimerizes with co-repressors in the absence of agonist ligands. Upon binding with RA, RAR or RXR dissociates with their co-repressors, recruits co-activator proteins, and promotes downstream gene transcription (Fig. 3). The RA signal is particularly critical for the generation of LTi cells in the fetal stage, largely by controlling RORγt expression (van de Pavert et al., 2014) (Fig. 3). Maternal retinoid level is thus required for setting up immune structures in the offspring. In the adult stage, RA is also reported to be associated with the migration of ILC1 and ILC3 cells in the gut as well as the IL-22 production by ILC3s (Kim et al., 2015; Mielke et al., 2013b) (Fig. 3).

Ikaros

Ikaros, together with Aiolos, Helios, Eos, etc., belongs to the Ikaros zinc finger transcription factor family. Ikaros is essential for regulating hematopoiesis through affecting CLP, pro-B, and NKp generation (Yoshida et al., 2010). Ikaros is also found to be crucial for fetal LTi cells as its deficiency leads to a defect in secondary lymphoid organogenesis (Schjerven et al., 2013). However, in postnatal ILC3 cells, Ikaros may repress by inhibiting AhR activity (Li et al., 2016) (Fig. 3). A Ikzf1 (gene encode Ikaros) deficiency can result in the expansion of ILC3s and elevated effector cytokine production. Thus, the regulatory effects of Ikaros on ILC3 development and function are stage specific.

ILC1-associated transcription factors

ILC1 cells were identified relatively late in comparison to other ILC family members. ILC1s are much more similar to cNK cells in many of their features, however they lack cNKs cytotoxic activity. IL-15 is required for the normal development and function of ILC1s (Klose et al., 2014). IL-12, in addition, can also promote ILC1 cells to secret effector cytokines IFNγ and TNFα (Fig. 4). Based on a lineage tracing study using PLZF fate-mapping mice, ILC1s and cNK cells originate from distinct progenitors (Constantinides et al., 2015). While the vast majority of ILC1 cells develop from PLZF-expressing common ILC progenitors, most cNK progenitors do not express PLZF. ILC1 and cNK cells can be distinguished in the periphery by certain surface markers. For example, in the liver, ILC1 cells are CD49a+DX5, whereas cNK cells are CD49aDX5+ (Sojka et al., 2014). However, these markers are not always reliable in other tissues. Another well-accepted way to distinguish these two populations is based on their expression of Eomes (Klose et al., 2014). ILC1 cells are Eomes but cNK cells are Eomes+. However, it is unclear whether some ILC1s may express or have expressed Eomes during their development.

Figure 4
ILC1-specific transcription factors. ILC1 is a newly confirmed group 1 ILC population that is distinct from cNK cells. The special functions of ILC1s and underlying regulatory mechanism still need further investigation. T-bet is the master transcriptional ...

Like Th1 cells, T-bet is the master regulator for ILC1 cells. T-bet is absolutely required for the generation of ILC1 but not for cNK cells (Daussy et al., 2014), although Tbx21 −/− cNK cells also display some abnormalities (Gordon et al., 2012). Besides its critical function during ILC1 development, T-bet is required for the maintenance of ILC1 cells and production of ILC1 effector cytokines (Klose et al., 2014) (Fig. 4).

Runx3

As we described above, Runx3 is essential for the development of ILC1 and ILC3, but not ILC2 cells. In committed ILC1 cells, Runx3 is also required for the survival of ILC1 cells due to its involvement in IL-15 signaling (Ebihara et al., 2015). IL-15 is particularly important for ILC1 cells as indicated by knockout studies. In mature ILC1s, the absence of Runx3 or its common obligatory partner Cbfb results in enhanced apoptosis, which is associated with the dysregulation of anti-apoptotic factors such as Bcl-2 (Fig. 4).

CONCLUSION

Transcriptional regulation underlies the functional actions of various lymphoid effectors and is the key to further understanding how the immune system works. However more comprehensive studies are still required to understand how recently identified ILC populations are generated and how their functions are controlled. Through the studies of select transcription factors, we have found that ILCs exhibit many similarities to CD4+ Th cells in terms of their development, maintenance, proliferation, and effector cytokine production. Beyond these similarities, the unique features of ILCs compared to those of Th cells, such as tissue residency, have recently drawn considerable attention. Despite considerable progress, there are still many challenges to address in order to understand the how ILCs and ILC-functions are ultimately regulated and controlled.

ACKNOWLEDGMENTS

We thank our colleague, Dr. Matthew Butcher, for his proofreading of this article. The work is supported by the Intramural Research Program of National Institute of Health (NIH), National Institute of Allergy and Infectious Disease (NIAID), NIH, USA.

ABBREVIATIONS

AhR, aryl hydrocarbon receptor; AIP, aryl hydrocarbon receptor interacting protein; ARNT, aryl hydrocarbon receptor nuclear translocator; ChILPs, common helper-like innate lymphocyte progenitors; CILP, common innate lymphoid progenitor; CLP, common lymphoid progenitor; cNK, conventional natural killer; DAMPs, damage-associated molecular patterns; ETPs, early T cell precursors; HLH, helix-loop-helix; HMG, high mobility group; ILCs, innate lymphoid cells; LTi, lymphoid tissue inducer; NCR, natural cytotoxicity receptor; PAMPs, pathogen-associated molecular patterns; PR, pattern recongnition; PRRs, pattern recognition receptors; RA, retinoic acid; RAR, retinoic acid receptor; RXR, retinoid X receptor; TCF1, T-cell factor 1; Th, T helper; TOX, thymocyte selection-associated HMG box protein.

COMPLIANCE WITH ETHICS GUIDELINES

The authors declare that there is no conflict of interest regarding the publication of this paper. This article does not contain any studies with human or animal subjects performed by any of the authors.

Contributor Information

Chao Zhong, vog.hin.diain@cgnohz.

Jinfang Zhu, vog.hin.diain@uhZfj.

References

  • Aliahmad P, Kaye J. Development of all CD4 T lineages requires nuclear factor TOX. J Exp Med. 2008;205:245–256. doi: 10.1084/jem.20071944. [PMC free article] [PubMed] [Cross Ref]
  • Aliahmad P, de la Torre B, Kaye J. Shared dependence on the DNA-binding factor TOX for the development of lymphoid tissue-inducer cell and NK cell lineages. Nat Immunol. 2010;11:945–952. doi: 10.1038/ni.1930. [PMC free article] [PubMed] [Cross Ref]
  • Artis D, Spits H. The biology of innate lymphoid cells. Nature. 2015;517:293–301. doi: 10.1038/nature14189. [PubMed] [Cross Ref]
  • Bando JK, Liang HE, Locksley RM. Identification and distribution of developing innate lymphoid cells in the fetal mouse intestine. Nat Immunol. 2015;16:153–160. doi: 10.1038/ni.3057. [PMC free article] [PubMed] [Cross Ref]
  • Bjorklund AK, Forkel M, Picelli S, Konya V, Theorell J, Friberg D, Sandberg R, Mjosberg J. The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing. Nat Immunol. 2016;17:451–460. doi: 10.1038/ni.3368. [PubMed] [Cross Ref]
  • Boos MD, Yokota Y, Eberl G, Kee BL. Mature natural killer cell and lymphoid tissue-inducing cell development requires Id2-mediated suppression of E protein activity. J Exp Med. 2007;204:1119–1130. doi: 10.1084/jem.20061959. [PMC free article] [PubMed] [Cross Ref]
  • Brubaker SW, Bonham KS, Zanoni I, Kagan JC. Innate immune pattern recognition: a cell biological perspective. Annu Rev Immunol. 2015;33:257–290. doi: 10.1146/annurev-immunol-032414-112240. [PMC free article] [PubMed] [Cross Ref]
  • Califano D, Cho JJ, Uddin MN, Lorentsen KJ, Yang Q, Bhandoola A, Li H, Avram D. Transcription factor Bcl11b controls identity and function of mature type 2 innate lymphoid cells. Immunity. 2015;43:354–368. doi: 10.1016/j.immuni.2015.07.005. [PMC free article] [PubMed] [Cross Ref]
  • Cella M, Fuchs A, Vermi W, Facchetti F, Otero K, Lennerz JK, Doherty JM, Mills JC, Colonna M. A human natural killer cell subset provides an innate source of IL-22 for mucosal immunity. Nature. 2009;457:722–725. doi: 10.1038/nature07537. [PMC free article] [PubMed] [Cross Ref]
  • Chea S, Schmutz S, Berthault C, Perchet T, Petit M, Burlen-Defranoux O, Goldrath AW, Rodewald HR, Cumano A, Golub R. Single-cell gene expression analyses reveal heterogeneous responsiveness of fetal innate lymphoid progenitors to notch signaling. Cell Rep. 2016;14:1500–1516. doi: 10.1016/j.celrep.2016.01.015. [PubMed] [Cross Ref]
  • Constantinides MG, McDonald BD, Verhoef PA, Bendelac A. A committed precursor to innate lymphoid cells. Nature. 2014;508:397–401. doi: 10.1038/nature13047. [PMC free article] [PubMed] [Cross Ref]
  • Constantinides MG, Gudjonson H, McDonald BD, Ishizuka IE, Verhoef PA, Dinner AR, Bendelac A. PLZF expression maps the early stages of ILC1 lineage development. Proc Natl Acad Sci USA. 2015;112:5123–5128. doi: 10.1073/pnas.1423244112. [PubMed] [Cross Ref]
  • Daussy C, Faure F, Mayol K, Viel S, Gasteiger G, Charrier E, Bienvenu J, Henry T, Debien E, Hasan UA, et al. T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow. J Exp Med. 2014;211:563–577. doi: 10.1084/jem.20131560. [PMC free article] [PubMed] [Cross Ref]
  • Dudakov JA, Hanash AM, van den Brink MR. Interleukin-22: immunobiology and pathology. Annu Rev Immunol. 2015;33:747–785. doi: 10.1146/annurev-immunol-032414-112123. [PMC free article] [PubMed] [Cross Ref]
  • Eberl G, Colonna M, Di Santo JP, McKenzie AN. Innate lymphoid cells. Innate lymphoid cells: a new paradigm in immunology. Science. 2015;348:aaa6566. doi: 10.1126/science.aaa6566. [PubMed] [Cross Ref]
  • Eberl G, Di Santo JP, Vivier E. The brave new world of innate lymphoid cells. Nat Immunol. 2015;16:1–5. doi: 10.1038/ni.3059. [PubMed] [Cross Ref]
  • Ebihara T, Song C, Ryu SH, Plougastel-Douglas B, Yang L, Levanon D, Groner Y, Bern MD, Stappenbeck TS, Colonna M, et al. Runx3 specifies lineage commitment of innate lymphoid cells. Nat Immunol. 2015;16:1124–1133. doi: 10.1038/ni.3272. [PMC free article] [PubMed] [Cross Ref]
  • Fallon PG, Ballantyne SJ, Mangan NE, Barlow JL, Dasvarma A, Hewett DR, McIlgorm A, Jolin HE, McKenzie AN. Identification of an interleukin (IL)-25-dependent cell population that provides IL-4, IL-5, and IL-13 at the onset of helminth expulsion. J Exp Med. 2006;203:1105–1116. doi: 10.1084/jem.20051615. [PMC free article] [PubMed] [Cross Ref]
  • Finke D. Fate and function of lymphoid tissue inducer cells. Curr Opin Immunol. 2005;17:144–150. doi: 10.1016/j.coi.2005.01.006. [PubMed] [Cross Ref]
  • Gascoyne DM, Long E, Veiga-Fernandes H, de Boer J, Williams O, Seddon B, Coles M, Kioussis D, Brady HJ. The basic leucine zipper transcription factor E4BP4 is essential for natural killer cell development. Nat Immunol. 2009;10:1118–1124. doi: 10.1038/ni.1787. [PubMed] [Cross Ref]
  • Gasteiger G, Fan X, Dikiy S, Lee SY, Rudensky AY. Tissue residency of innate lymphoid cells in lymphoid and nonlymphoid organs. Science. 2015;350:981–985. doi: 10.1126/science.aac9593. [PMC free article] [PubMed] [Cross Ref]
  • Germain RN. T-cell development and the CD4CD8 lineage decision. Nat Rev Immunol. 2002;2:309–322. doi: 10.1038/nri798. [PubMed] [Cross Ref]
  • Gordon SM, Chaix J, Rupp LJ, Wu J, Madera S, Sun JC, Lindsten T, Reiner SL. The transcription factors T-bet and Eomes control key checkpoints of natural killer cell maturation. Immunity. 2012;36:55–67. doi: 10.1016/j.immuni.2011.11.016. [PMC free article] [PubMed] [Cross Ref]
  • Goto Y, Obata T, Kunisawa J, Sato S, Ivanov II, Lamichhane A, Takeyama N, Kamioka M, Sakamoto M, Matsuki T, et al. Innate lymphoid cells regulate intestinal epithelial cell glycosylation. Science. 2014;345:1254009. doi: 10.1126/science.1254009. [PMC free article] [PubMed] [Cross Ref]
  • Gury-BenAri M, Thaiss CA, Serafini N, Winter DR, Giladi A, Lara-Astiaso D, Levy M, Salame TM, Weiner A, David E, et al. The spectrum and regulatory landscape of intestinal innate lymphoid cells are shaped by the microbiome. Cell. 2016;166(1231–1246):e1213. [PubMed]
  • Halim TY, MacLaren A, Romanish MT, Gold MJ, McNagny KM, Takei F. Retinoic-acid-receptor-related orphan nuclear receptor alpha is required for natural helper cell development and allergic inflammation. Immunity. 2012;37:463–474. doi: 10.1016/j.immuni.2012.06.012. [PubMed] [Cross Ref]
  • Halim TY, Steer CA, Matha L, Gold MJ, Martinez-Gonzalez I, McNagny KM, McKenzie AN, Takei F. Group 2 innate lymphoid cells are critical for the initiation of adaptive T helper 2 cell-mediated allergic lung inflammation. Immunity. 2014;40:425–435. doi: 10.1016/j.immuni.2014.01.011. [PMC free article] [PubMed] [Cross Ref]
  • Halim TY, Hwang YY, Scanlon ST, Zaghouani H, Garbi N, Fallon PG, McKenzie AN. Group 2 innate lymphoid cells license dendritic cells to potentiate memory TH2 cell responses. Nat Immunol. 2016;17:57–64. doi: 10.1038/ni.3294. [PMC free article] [PubMed] [Cross Ref]
  • Hanash AM, Dudakov JA, Hua G, O’Connor MH, Young LF, Singer NV, West ML, Jenq RR, Holland AM, Kappel LW, et al. Interleukin-22 protects intestinal stem cells from immune-mediated tissue damage and regulates sensitivity to graft versus host disease. Immunity. 2012;37:339–350. doi: 10.1016/j.immuni.2012.05.028. [PMC free article] [PubMed] [Cross Ref]
  • Hepworth MR, Monticelli LA, Fung TC, Ziegler CG, Grunberg S, Sinha R, Mantegazza AR, Ma HL, Crawford A, Angelosanto JM, et al. Innate lymphoid cells regulate CD4+ T-cell responses to intestinal commensal bacteria. Nature. 2013;498:113–117. doi: 10.1038/nature12240. [PMC free article] [PubMed] [Cross Ref]
  • Hepworth MR, Fung TC, Masur SH, Kelsen JR, McConnell FM, Dubrot J, Withers DR, Hugues S, Farrar MA, Reith W, et al. Immune tolerance. Group 3 innate lymphoid cells mediate intestinal selection of commensal bacteria-specific CD4(+) T cells. Science. 2015;348:1031–1035. doi: 10.1126/science.aaa4812. [PMC free article] [PubMed] [Cross Ref]
  • Ho IC, Tai TS, Pai SY. GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 2009;9:125–135. doi: 10.1038/nri2476. [PMC free article] [PubMed] [Cross Ref]
  • Hoyler T, Klose CS, Souabni A, Turqueti-Neves A, Pfeifer D, Rawlins EL, Voehringer D, Busslinger M, Diefenbach A. The transcription factor GATA-3 controls cell fate and maintenance of type 2 innate lymphoid cells. Immunity. 2012;37:634–648. doi: 10.1016/j.immuni.2012.06.020. [PMC free article] [PubMed] [Cross Ref]
  • Ikawa T, Hirose S, Masuda K, Kakugawa K, Satoh R, Shibano-Satoh A, Kominami R, Katsura Y, Kawamoto H. An essential developmental checkpoint for production of the T cell lineage. Science. 2010;329:93–96. doi: 10.1126/science.1188995. [PubMed] [Cross Ref]
  • Ishizuka IE, Chea S, Gudjonson H, Constantinides MG, Dinner AR, Bendelac A, Golub R. Single-cell analysis defines the divergence between the innate lymphoid cell lineage and lymphoid tissue-inducer cell lineage. Nat Immunol. 2016;17:269–276. doi: 10.1038/ni.3344. [PMC free article] [PubMed] [Cross Ref]
  • Kee BL. E and ID proteins branch out. Nat Rev Immunol. 2009;9:175–184. doi: 10.1038/nri2507. [PubMed] [Cross Ref]
  • Kim MH, Taparowsky EJ, Kim CH. Retinoic acid differentially regulates the migration of innate lymphoid cell subsets to the gut. Immunity. 2015;43:107–119. doi: 10.1016/j.immuni.2015.06.009. [PMC free article] [PubMed] [Cross Ref]
  • Kiss EA, Vonarbourg C, Kopfmann S, Hobeika E, Finke D, Esser C, Diefenbach A. Natural aryl hydrocarbon receptor ligands control organogenesis of intestinal lymphoid follicles. Science. 2011;334:1561–1565. doi: 10.1126/science.1214914. [PubMed] [Cross Ref]
  • Klose CS, Kiss EA, Schwierzeck V, Ebert K, Hoyler T, d’Hargues Y, Goppert N, Croxford AL, Waisman A, Tanriver Y, Diefenbach A. A T-bet gradient controls the fate and function of CCR6-RORgammat+ innate lymphoid cells. Nature. 2013;494:261–265. doi: 10.1038/nature11813. [PubMed] [Cross Ref]
  • Klose CS, Flach M, Mohle L, Rogell L, Hoyler T, Ebert K, Fabiunke C, Pfeifer D, Sexl V, Fonseca-Pereira D, et al. Differentiation of type 1 ILCs from a common progenitor to all helper-like innate lymphoid cell lineages. Cell. 2014;157:340–356. doi: 10.1016/j.cell.2014.03.030. [PubMed] [Cross Ref]
  • Lee JS, Cella M, McDonald KG, Garlanda C, Kennedy GD, Nukaya M, Mantovani A, Kopan R, Bradfield CA, Newberry RD, Colonna M. AHR drives the development of gut ILC22 cells and postnatal lymphoid tissues via pathways dependent on and independent of Notch. Nat Immunol. 2011;13:144–151. doi: 10.1038/ni.2187. [PMC free article] [PubMed] [Cross Ref]
  • Lee MW, Odegaard JI, Mukundan L, Qiu Y, Molofsky AB, Nussbaum JC, Yun K, Locksley RM, Chawla A. Activated type 2 innate lymphoid cells regulate beige fat biogenesis. Cell. 2015;160:74–87. doi: 10.1016/j.cell.2014.12.011. [PMC free article] [PubMed] [Cross Ref]
  • Li S, Heller JJ, Bostick JW, Lee A, Schjerven H, Kastner P, Chan S, Chen ZE, Zhou L. Ikaros inhibits group 3 innate lymphoid cell development and function by suppressing the aryl hydrocarbon receptor pathway. Immunity. 2016;45:185–197. doi: 10.1016/j.immuni.2016.06.027. [PubMed] [Cross Ref]
  • Lindemans CA, Calafiore M, Mertelsmann AM, O’Connor MH, Dudakov JA, Jenq RR, Velardi E, Young LF, Smith OM, Lawrence G, et al. Interleukin-22 promotes intestinal-stem-cell-mediated epithelial regeneration. Nature. 2015;528:560–564. doi: 10.1038/nature16460. [PMC free article] [PubMed] [Cross Ref]
  • Lo BC, Gold MJ, Hughes MR, Antignano F, Valdez Y, Zaph C, Harder KW, McNagny KM. The orphan nuclear receptor RORα and group 3 innate lymphoid cells drive fibrosis in a mouse model of Crohn’s disease. Sci Immunol. 2016;1(3):aaf8864. doi: 10.1126/sciimmunol.aaf8864. [Cross Ref]
  • Luci C, Reynders A, Ivanov II, Cognet C, Chiche L, Chasson L, Hardwigsen J, Anguiano E, Banchereau J, Chaussabel D, et al. Influence of the transcription factor RORgammat on the development of NKp46+ cell populations in gut and skin. Nat Immunol. 2009;10:75–82. doi: 10.1038/ni.1681. [PubMed] [Cross Ref]
  • Mielke LA, Groom JR, Rankin LC, Seillet C, Masson F, Putoczki T, Belz GT. TCF-1 controls ILC2 and NKp46+RORgammat+ innate lymphocyte differentiation and protection in intestinal inflammation. J Immunol. 2013;191:4383–4391. doi: 10.4049/jimmunol.1301228. [PubMed] [Cross Ref]
  • Mielke LA, Jones SA, Raverdeau M, Higgs R, Stefanska A, Groom JR, Misiak A, Dungan LS, Sutton CE, Streubel G, et al. Retinoic acid expression associates with enhanced IL-22 production by gammadelta T cells and innate lymphoid cells and attenuation of intestinal inflammation. J Exp Med. 2013;210:1117–1124. doi: 10.1084/jem.20121588. [PMC free article] [PubMed] [Cross Ref]
  • Monticelli LA, Sonnenberg GF, Abt MC, Alenghat T, Ziegler CG, Doering TA, Angelosanto JM, Laidlaw BJ, Yang CY, Sathaliyawala T, et al. Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus. Nat Immunol. 2011;12:1045–1054. doi: 10.1038/ni.2131. [PMC free article] [PubMed] [Cross Ref]
  • Moro K, Yamada T, Tanabe M, Takeuchi T, Ikawa T, Kawamoto H, Furusawa J, Ohtani M, Fujii H, Koyasu S. Innate production of T(H)2 cytokines by adipose tissue-associated c-Kit(+)Sca-1(+) lymphoid cells. Nature. 2010;463:540–544. doi: 10.1038/nature08636. [PubMed] [Cross Ref]
  • Neill DR, Wong SH, Bellosi A, Flynn RJ, Daly M, Langford TK, Bucks C, Kane CM, Fallon PG, Pannell R, et al. Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity. Nature. 2010;464:1367–1370. doi: 10.1038/nature08900. [PMC free article] [PubMed] [Cross Ref]
  • Nussbaum JC, Van Dyken SJ, von Moltke J, Cheng LE, Mohapatra A, Molofsky AB, Thornton EE, Krummel MF, Chawla A, Liang HE, Locksley RM. Type 2 innate lymphoid cells control eosinophil homeostasis. Nature. 2013;502:245–248. doi: 10.1038/nature12526. [PMC free article] [PubMed] [Cross Ref]
  • Oliphant CJ, Hwang YY, Walker JA, Salimi M, Wong SH, Brewer JM, Englezakis A, Barlow JL, Hams E, Scanlon ST, et al. MHCII-mediated dialog between group 2 innate lymphoid cells and CD4(+) T cells potentiates type 2 immunity and promotes parasitic helminth expulsion. Immunity. 2014;41:283–295. doi: 10.1016/j.immuni.2014.06.016. [PMC free article] [PubMed] [Cross Ref]
  • O’Shea JJ, Paul WE. Mechanisms underlying lineage commitment and plasticity of helper CD4+ T cells. Science. 2010;327:1098–1102. doi: 10.1126/science.1178334. [PMC free article] [PubMed] [Cross Ref]
  • Pai SY, Truitt ML, Ting CN, Leiden JM, Glimcher LH, Ho IC. Critical roles for transcription factor GATA-3 in thymocyte development. Immunity. 2003;19:863–875. doi: 10.1016/S1074-7613(03)00328-5. [PubMed] [Cross Ref]
  • Pantelyushin S, Haak S, Ingold B, Kulig P, Heppner FL, Navarini AA, Becher B. Rorgammat+ innate lymphocytes and gammadelta T cells initiate psoriasiform plaque formation in mice. J Clin Invest. 2012;122:2252–2256. doi: 10.1172/JCI61862. [PMC free article] [PubMed] [Cross Ref]
  • Pickard JM, Maurice CF, Kinnebrew MA, Abt MC, Schenten D, Golovkina TV, Bogatyrev SR, Ismagilov RF, Pamer EG, Turnbaugh PJ, Chervonsky AV. Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness. Nature. 2014;514:638–641. doi: 10.1038/nature13823. [PMC free article] [PubMed] [Cross Ref]
  • Possot C, Schmutz S, Chea S, Boucontet L, Louise A, Cumano A, Golub R. Notch signaling is necessary for adult, but not fetal, development of RORgammat(+) innate lymphoid cells. Nat Immunol. 2011;12:949–958. doi: 10.1038/ni.2105. [PubMed] [Cross Ref]
  • Price AE, Liang HE, Sullivan BM, Reinhardt RL, Eisley CJ, Erle DJ, Locksley RM. Systemically dispersed innate IL-13-expressing cells in type 2 immunity. Proc Natl Acad Sci U S A. 2010;107:11489–11494. doi: 10.1073/pnas.1003988107. [PubMed] [Cross Ref]
  • Qiu J, Heller JJ, Guo X, Chen ZM, Fish K, Fu YX, Zhou L. The aryl hydrocarbon receptor regulates gut immunity through modulation of innate lymphoid cells. Immunity. 2012;36:92–104. doi: 10.1016/j.immuni.2011.11.011. [PMC free article] [PubMed] [Cross Ref]
  • Radtke F, MacDonald HR, Tacchini-Cottier F. Regulation of innate and adaptive immunity by Notch. Nat Rev Immunol. 2013;13:427–437. doi: 10.1038/nri3445. [PubMed] [Cross Ref]
  • Saenz SA, Siracusa MC, Monticelli LA, Ziegler CG, Kim BS, Brestoff JR, Peterson LW, Wherry EJ, Goldrath AW, Bhandoola A, Artis D. IL-25 simultaneously elicits distinct populations of innate lymphoid cells and multipotent progenitor type 2 (MPPtype2) cells. J Exp Med. 2013;210:1823–1837. doi: 10.1084/jem.20122332. [PMC free article] [PubMed] [Cross Ref]
  • Samson SI, Richard O, Tavian M, Ranson T, Vosshenrich CA, Colucci F, Buer J, Grosveld F, Godin I, Di Santo JP. GATA-3 promotes maturation, IFN-gamma production, and liver-specific homing of NK cells. Immunity. 2003;19:701–711. doi: 10.1016/S1074-7613(03)00294-2. [PubMed] [Cross Ref]
  • Sanos SL, Bui VL, Mortha A, Oberle K, Heners C, Johner C, Diefenbach A. RORgammat and commensal microflora are required for the differentiation of mucosal interleukin 22-producing NKp46+ cells. Nat Immunol. 2009;10:83–91. doi: 10.1038/ni.1684. [PMC free article] [PubMed] [Cross Ref]
  • Schjerven H, McLaughlin J, Arenzana TL, Frietze S, Cheng D, Wadsworth SE, Lawson GW, Bensinger SJ, Farnham PJ, Witte ON, Smale ST. Selective regulation of lymphopoiesis and leukemogenesis by individual zinc fingers of Ikaros. Nat Immunol. 2013;14:1073–1083. doi: 10.1038/ni.2707. [PMC free article] [PubMed] [Cross Ref]
  • Sciume G, Hirahara K, Takahashi H, Laurence A, Villarino AV, Singleton KL, Spencer SP, Wilhelm C, Poholek AC, Vahedi G, et al. Distinct requirements for T-bet in gut innate lymphoid cells. J Exp Med. 2012;209:2331–2338. doi: 10.1084/jem.20122097. [PMC free article] [PubMed] [Cross Ref]
  • Seehus CR, Aliahmad P, de la Torre B, Iliev ID, Spurka L, Funari VA, Kaye J. The development of innate lymphoid cells requires TOX-dependent generation of a common innate lymphoid cell progenitor. Nat Immunol. 2015;16:599–608. doi: 10.1038/ni.3168. [PMC free article] [PubMed] [Cross Ref]
  • Seillet C, Rankin LC, Groom JR, Mielke LA, Tellier J, Chopin M, Huntington ND, Belz GT, Carotta S. Nfil3 is required for the development of all innate lymphoid cell subsets. J Exp Med. 2014;211:1733–1740. doi: 10.1084/jem.20140145. [PMC free article] [PubMed] [Cross Ref]
  • Serafini N, Klein Wolterink RG, Satoh-Takayama N, Xu W, Vosshenrich CA, Hendriks RW, Di Santo JP. Gata3 drives development of RORgammat+ group 3 innate lymphoid cells. J Exp Med. 2014;211:199–208. doi: 10.1084/jem.20131038. [PMC free article] [PubMed] [Cross Ref]
  • Sojka DK, Plougastel-Douglas B, Yang L, Pak-Wittel MA, Artyomov MN, Ivanova Y, Zhong C, Chase JM, Rothman PB, Yu J, et al. Tissue-resident natural killer (NK) cells are cell lineages distinct from thymic and conventional splenic NK cells. Elife. 2014;3:e01659. doi: 10.7554/eLife.01659. [PMC free article] [PubMed] [Cross Ref]
  • Spooner CJ, Lesch J, Yan D, Khan AA, Abbas A, Ramirez-Carrozzi V, Zhou M, Soriano R, Eastham-Anderson J, Diehl L, et al. Specification of type 2 innate lymphocytes by the transcriptional determinant Gfi1. Nat Immunol. 2013;14:1229–1236. doi: 10.1038/ni.2743. [PubMed] [Cross Ref]
  • Steinke FC, Yu S, Zhou X, He B, Yang W, Zhou B, Kawamoto H, Zhu J, Tan K, Xue HH. TCF-1 and LEF-1 act upstream of Th-POK to promote the CD4(+) T cell fate and interact with Runx3 to silence Cd4 in CD8(+) T cells. Nat Immunol. 2014;15:646–656. doi: 10.1038/ni.2897. [PMC free article] [PubMed] [Cross Ref]
  • Tachibana M, Tenno M, Tezuka C, Sugiyama M, Yoshida H, Taniuchi I. Runx1/Cbfbeta2 complexes are required for lymphoid tissue inducer cell differentiation at two developmental stages. J Immunol. 2011;186:1450–1457. doi: 10.4049/jimmunol.1000162. [PubMed] [Cross Ref]
  • Taghon T, Yui MA, Rothenberg EV. Mast cell lineage diversion of T lineage precursors by the essential T cell transcription factor GATA-3. Nat Immunol. 2007;8:845–855. doi: 10.1038/ni1486. [PMC free article] [PubMed] [Cross Ref]
  • Takatori H, Kanno Y, Watford WT, Tato CM, Weiss G, Ivanov II, Littman DR, O’Shea JJ. Lymphoid tissue inducer-like cells are an innate source of IL-17 and IL-22. J Exp Med. 2009;206:35–41. doi: 10.1084/jem.20072713. [PMC free article] [PubMed] [Cross Ref]
  • van de Pavert SA, Ferreira M, Domingues RG, Ribeiro H, Molenaar R, Moreira-Santos L, Almeida FF, Ibiza S, Barbosa I, Goverse G, et al. Maternal retinoids control type 3 innate lymphoid cells and set the offspring immunity. Nature. 2014;508:123–127. doi: 10.1038/nature13158. [PMC free article] [PubMed] [Cross Ref]
  • Van Dyken SJ, Nussbaum JC, Lee J, Molofsky AB, Liang HE, Pollack JL, Gate RE, Haliburton GE, Ye CJ, Marson A, et al. A tissue checkpoint regulates type 2 immunity. Nat Immunol. 2016;17:1381–1387. doi: 10.1038/ni.3582. [PMC free article] [PubMed] [Cross Ref]
  • Verykokakis M, Zook EC, Kee BL. ID’ing innate and innate-like lymphoid cells. Immunol Rev. 2014;261:177–197. doi: 10.1111/imr.12203. [PMC free article] [PubMed] [Cross Ref]
  • Walker JA, Oliphant CJ, Englezakis A, Yu Y, Clare S, Rodewald HR, Belz G, Liu P, Fallon PG, McKenzie AN. Bcl11b is essential for group 2 innate lymphoid cell development. J Exp Med. 2015;212:875–882. doi: 10.1084/jem.20142224. [PMC free article] [PubMed] [Cross Ref]
  • Wang L, Wildt KF, Zhu J, Zhang X, Feigenbaum L, Tessarollo L, Paul WE, Fowlkes BJ, Bosselut R. Distinct functions for the transcription factors GATA-3 and ThPOK during intrathymic differentiation of CD4(+) T cells. Nat Immunol. 2008;9:1122–1130. doi: 10.1038/ni.1647. [PMC free article] [PubMed] [Cross Ref]
  • Weber BN, Chi AW, Chavez A, Yashiro-Ohtani Y, Yang Q, Shestova O, Bhandoola A. A critical role for TCF-1 in T-lineage specification and differentiation. Nature. 2011;476:63–68. doi: 10.1038/nature10279. [PMC free article] [PubMed] [Cross Ref]
  • Wilhelm C, Hirota K, Stieglitz B, Van Snick J, Tolaini M, Lahl K, Sparwasser T, Helmby H, Stockinger B. An IL-9 fate reporter demonstrates the induction of an innate IL-9 response in lung inflammation. Nat Immunol. 2011;12:1071–1077. doi: 10.1038/ni.2133. [PMC free article] [PubMed] [Cross Ref]
  • Wong SH, Walker JA, Jolin HE, Drynan LF, Hams E, Camelo A, Barlow JL, Neill DR, Panova V, Koch U, et al. Transcription factor RORalpha is critical for nuocyte development. Nat Immunol. 2012;13:229–236. doi: 10.1038/ni.2208. [PMC free article] [PubMed] [Cross Ref]
  • Woolf E, Xiao C, Fainaru O, Lotem J, Rosen D, Negreanu V, Bernstein Y, Goldenberg D, Brenner O, Berke G, et al. Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis. Proc Natl Acad Sci USA. 2003;100:7731–7736. doi: 10.1073/pnas.1232420100. [PubMed] [Cross Ref]
  • Xu W, Domingues RG, Fonseca-Pereira D, Ferreira M, Ribeiro H, Lopez-Lastra S, Motomura Y, Moreira-Santos L, Bihl F, Braud V, et al. NFIL3 orchestrates the emergence of common helper innate lymphoid cell precursors. Cell Rep. 2015;10:2043–2054. doi: 10.1016/j.celrep.2015.02.057. [PubMed] [Cross Ref]
  • Yagi R, Zhong C, Northrup DL, Yu F, Bouladoux N, Spencer S, Hu G, Barron L, Sharma S, Nakayama T, et al. The transcription factor GATA3 is critical for the development of all IL-7Ralpha-expressing innate lymphoid cells. Immunity. 2014;40:378–388. doi: 10.1016/j.immuni.2014.01.012. [PMC free article] [PubMed] [Cross Ref]
  • Yang Q, Monticelli LA, Saenz SA, Chi AW, Sonnenberg GF, Tang J, De Obaldia ME, Bailis W, Bryson JL, Toscano K, et al. T cell factor 1 is required for group 2 innate lymphoid cell generation. Immunity. 2013;38:694–704. doi: 10.1016/j.immuni.2012.12.003. [PMC free article] [PubMed] [Cross Ref]
  • Yang Q, Li F, Harly C, Xing S, Ye L, Xia X, Wang H, Wang X, Yu S, Zhou X, et al. TCF-1 upregulation identifies early innate lymphoid progenitors in the bone marrow. Nat Immunol. 2015;16:1044–1050. doi: 10.1038/ni.3248. [PMC free article] [PubMed] [Cross Ref]
  • Yokota Y, Mansouri A, Mori S, Sugawara S, Adachi S, Nishikawa S, Gruss P. Development of peripheral lymphoid organs and natural killer cells depends on the helix-loop-helix inhibitor Id2. Nature. 1999;397:702–706. doi: 10.1038/17812. [PubMed] [Cross Ref]
  • Yoshida T, Ng SY, Georgopoulos K. Awakening lineage potential by Ikaros-mediated transcriptional priming. Curr Opin Immunol. 2010;22:154–160. doi: 10.1016/j.coi.2010.02.011. [PMC free article] [PubMed] [Cross Ref]
  • Yu X, Wang Y, Deng M, Li Y, Ruhn KA, Zhang CC, Hooper LV. The basic leucine zipper transcription factor NFIL3 directs the development of a common innate lymphoid cell precursor. Elife. 2014;3:e04406. doi: 10.7554/eLife.04406. [PMC free article] [PubMed] [Cross Ref]
  • Yu Y, Wang C, Clare S, Wang J, Lee SC, Brandt C, Burke S, Lu L, He D, Jenkins NA, et al. The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development. J Exp Med. 2015;212:865–874. doi: 10.1084/jem.20142318. [PMC free article] [PubMed] [Cross Ref]
  • Yu Y, Tsang JC, Wang C, Clare S, Wang J, Chen X, Brandt C, Kane L, Campos LS, Lu L, et al. Single-cell RNA-seq identifies a PD-1hi ILC progenitor and defines its development pathway. Nature. 2016;539:102–106. doi: 10.1038/nature20105. [PubMed] [Cross Ref]
  • Yui MA, Rothenberg EV. Developmental gene networks: a triathlon on the course to T cell identity. Nat Rev Immunol. 2014;14:529–545. doi: 10.1038/nri3702. [PMC free article] [PubMed] [Cross Ref]
  • Zhong C, Zhu J. Bcl11b drives the birth of ILC2 innate lymphocytes. J Exp Med. 2015;212:828. doi: 10.1084/jem.2126insight1. [PMC free article] [PubMed] [Cross Ref]
  • Zhong C, Zhu J. Transcriptional regulatory network for the development of innate lymphoid cells. Mediat Inflamm. 2015;2015:264502. [PMC free article] [PubMed]
  • Zhong C, Cui K, Wilhelm C, Hu G, Mao K, Belkaid Y, Zhao K, Zhu J. Group 3 innate lymphoid cells continuously require the transcription factor GATA-3 after commitment. Nat Immunol. 2016;17:169–178. doi: 10.1038/ni.3318. [PMC free article] [PubMed] [Cross Ref]
  • Zhou L. AHR Function in Lymphocytes: Emerging Concepts. Trends Immunol. 2016;37:17–31. doi: 10.1016/j.it.2015.11.007. [PMC free article] [PubMed] [Cross Ref]
  • Zhu J, Guo L, Min B, Watson CJ, Hu-Li J, Young HA, Tsichlis PN, Paul WE. Growth factor independent-1 induced by IL-4 regulates Th2 cell proliferation. Immunity. 2002;16:733–744. doi: 10.1016/S1074-7613(02)00317-5. [PubMed] [Cross Ref]
  • Zhu J, Jankovic D, Grinberg A, Guo L, Paul WE. Gfi-1 plays an important role in IL-2-mediated Th2 cell expansion. Proc Natl Acad Sci USA. 2006;103:18214–18219. doi: 10.1073/pnas.0608981103. [PubMed] [Cross Ref]
  • Zook EC, Kee BL. Development of innate lymphoid cells. Nat Immunol. 2016;17:775–782. doi: 10.1038/ni.3481. [PubMed] [Cross Ref]
  • Zook EC, Ramirez K, Guo X, van der Voort G, Sigvardsson M, Svensson EC, Fu YX, Kee BL. The ETS1 transcription factor is required for the development and cytokine-induced expansion of ILC2. J Exp Med. 2016;213:687–696. doi: 10.1084/jem.20150851. [PMC free article] [PubMed] [Cross Ref]

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