PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of springeropenLink to Publisher's site
Familial Cancer
 
Fam Cancer. 2017; 16(2): 271–277.
Published online 2016 November 8. doi:  10.1007/s10689-016-9943-z
PMCID: PMC5357498

Uptake of prenatal diagnostic testing for retinoblastoma compared to other hereditary cancer syndromes in the Netherlands

Abstract

Since the 1980s the genetic cause of many hereditary tumor syndromes has been elucidated. As a consequence, carriers of a deleterious mutation in these genes may opt for prenatal diagnoses (PND). We studied the uptake of prenatal diagnosis for five hereditary cancer syndromes in the Netherlands. Uptake for retinoblastoma (Rb) was compared with uptake for Von Hippel–Lindau disease (VHL), Li–Fraumeni syndrome (LFS), familial adenomatous polyposis (FAP), and hereditary breast ovarian cancer (HBOC). A questionnaire was completed by all nine DNA-diagnostic laboratories assessing the number of independent mutation-positive families identified from the start of diagnostic testing until May 2013, and the number of PNDs performed for these syndromes within these families. Of 187 families with a known Rb-gene mutation, 22 had performed PND (11.8%), this was significantly higher than uptake for FAP (1.6%) and HBOC (<0.2%). For VHL (6.5%) and LFS (4.9%) the difference was not statistically significant. PND for Rb started 3 years after introduction of diagnostic DNA testing and remained stable over the years. For the other cancer syndromes PND started 10–15 years after the introduction and uptake for PND showed an increase after 2009. We conclude that uptake of PND for Rb was significantly higher than for FAP and HBOC, but not different from VHL and LFS. Early onset, high penetrance, lack of preventive surgery and perceived burden of disease may explain these differences.

Keywords: Retinoblastoma, Prenatal diagnosis, Von Hippel–Lindau disease, Li–Fraumeni syndrome, Familial adenomatous polyposis, Hereditary breast ovarian cancer

Introduction

Approximately 5% of all cancers are caused by a genetic predisposition, with the mode of inheritance being mainly autosomal dominant. In the past 30 years the genetic cause of many hereditary cancer syndromes has been unravelled. Knowledge of the genetic predisposition can aid early diagnosis and management of cancer for affected mutation carriers. For unaffected family members, presymptomatic DNA diagnosis of the disease-associated mutation may enable informed choices about cancer screening or risk reduction strategies, including preventive surgery. Cancer genetic testing, however, can also affect reproductive decisions of mutation carriers [15]. Reproductive options for couples at risk of having a child with a cancer risk predisposition may be: refraining from having children or accepting the risk, adoption or gamete donation. Other options may include preimplantation genetic diagnosis (PGD) (i.e. in vitro fertilization, with genetic testing of 1 or 2 cells of the embryo and transfer of unaffected embryos to the uterus) and prenatal diagnostic testing for the deleterious cancer gene mutation with the option to terminate the pregnancy in the case of a carrier foetus. Prenatal diagnosis (PND) and PGD for hereditary cancer syndromes were described as early as 1988 and 1998, respectively [6, 7]. Legal aspects of these two techniques, and thus availability, differ across countries; e.g. in some countries PND followed by abortion is not allowed, whereas in other countries PGD is prohibited [8, 9]. Access to PND and/or PGD is also limited in some countries because they are not covered by health insurances [9]. Both in society and in medical literature, PND and PGD for hereditary cancer syndromes have led to ethical, social and legal discussions [1015]. Issues under debate are that many cancer-predisposing mutations have incomplete penetrance and that the onset of disease often does not occur until early adulthood. Furthermore, some argue that through the early detection of cancer or preventive surgery, the disease may be managed without a substantial effect on quality of life [15]. Arguments put forward in favour of offering PND and PGD are that preventive surgery may have a large impact on psychosocial well-being [16] and that families with hereditary cancer syndromes are burdened by their increased risk and deserve the same choices as families with other high-risk hereditary diseases [12, 17].

PND with the intention to terminate the pregnancy of an affected child is likely to reflect the perceived burden of the disease, i.e. future parents will only consider PND when they perceive the disease to be severe and wish to prevent their child from suffering [14]. In the Netherlands, PND for most hereditary cancers is offered as a reproductive option after extensive and careful consultation of the future parents with the clinical geneticist, a psychosocial worker and the gynaecologist [18, 19]. In several countries, PND for cancer syndromes has been performed, as listed in a review from 2006 [1]. However, most of the studies from this review are case descriptions, and papers on consecutive series on the uptake of PND for cancer are sparse.

We previously reported on reproductive decisions of couples at risk of having a child with retinoblastoma (Rb), a rare type of eye cancer in early childhood [3, 4]. Several couples who participated in these studies reported that they had chosen PND to prevent the birth of an affected child with Rb. The present study was conducted to determine how many families have used PND as a reproductive option for Rb since DNA diagnosis for Rb became available in the Netherlands in 1990. To put these data into perspective, we compared the use of PND for Rb to the uptake of PND for four other cancer syndromes with autosomal dominant inheritance.

Methods

Design

Comprehensive retrospective study in all (nine) academic diagnostic DNA laboratories within the Netherlands.

Choice of hereditary cancer syndromes

A comparison was made between Rb and four autosomal dominantly inherited cancer syndromes, i.e. Von Hippel–Lindau disease (VHL), Li–Fraumeni syndrome (LFS), familial adenomatous polyposis (FAP) and BRCA1- and BRCA2-related hereditary breast ovarian cancer (HBOC). These syndromes were selected as examples of cancer syndromes that may have cancer onset in early childhood (VHL, LFS), adolescence (FAP) or in adult life only (HBOC). Cancer syndromes with <40 families registered at the nine diagnostic DNA laboratories within the Netherlands were not considered due to presumed lack of power in the comparisons. Information on the different cancer syndromes is provided in Box Box11.

Box 1
Main characteristics of the hereditary cancer syndromes

Genetic counselling and DNA testing

In the Netherlands, costs for genetic counselling and DNA testing are covered within the national health service, and exclusively carried out by the DNA laboratories of the departments of clinical genetics of the university hospitals. All patients or their parents had at least one informative counselling session at a family cancer clinic with a genetic counsellor before DNA testing was performed. According to standard procedures, oral and written information about the cancer syndrome and the test results were provided. Reproductive options will have been discussed when the at-risk counselee was in the reproductive age.

DNA testing of the different cancer syndromes are apportioned among the nine laboratories; while some genes are diagnostically tested in nearly all laboratories (e.g. BRCA1/2), others are tested in only one (e.g. RB1).When a pathogenic mutation is detected within a family, DNA testing of subsequent family members will be performed in the laboratory in which the initial genetic diagnosis was made. Therefore the likelihood that family members are tested in two different laboratories is small. All laboratories use databases to keep track of family relationships and test results, including prenatal diagnostic tests.

For this study, a family is defined as all related mutation-positive family members from one family. The age and gender of the mutation carriers have not been registered.

The study has been approved by the Medical Ethics Review Committee of VU University Medical Center (VUMC) Amsterdam and was conducted in accordance with the principles of the Helsinki declaration.

Questionnaire

For this retrospective study, molecular geneticists of the nine DNA laboratories in the Netherlands were asked the same questions for all five cancer syndromes in May 2013 (end of inclusion):

  1. When did DNA diagnostic testing start in your laboratory?
  2. How many mutation-positive families are known in your laboratory?
  3. How many families have opted for PND since DNA testing became available?
  4. What was the date of each PND?
  5. How many times has PND been performed per family?

The laboratory was re-contacted when necessary.

National Retinoblastoma Treatment Center

Since 1991, all newly diagnosed Dutch Rb patients are being treated in the National Retinoblastoma Treatment Center at the VUMC in Amsterdam. This means that the majority of patients and their parents visited the clinical genetics department of VUMC for counselling for Rb. For this study, we registered which clinical genetics department in the Netherlands had requested PND for Rb. For the other hereditary cancer syndromes, there is no central treatment centre, so (pre-PND) genetic counselling was performed in all nine clinical genetics departments.

Statistical analysis

To test whether PND was performed more or less often in Rb families than in families with each of the other hereditary cancer syndromes, two-sided Fisher’s exact tests were used. A Bonferroni multiple testing correction was applied for the number of tests that were performed (uptake for Rb was compared with four hereditary cancer syndromes); p values <0.05/4 = 0.0125 were considered significant.

Results

Table 1 shows the five hereditary cancer syndromes, the number of mutation-positive families identified in the Netherlands, the total number of PNDs performed per cancer syndrome, and the number of couples that performed PND. PND was performed 35 times for Rb by 22 couples from 22 mutation-positive families (11.8% of 187 mutation-positive families). The percentages of families opting for PND for the other cancer syndromes ranged from <0.2% (HBOC) to 6.5% for VHL.

Table 1
Cancer syndromes with number of families known in the Netherlands, number of PNDs and comparison with number of PNDs for retinoblastoma

A significant difference in number of PNDs was seen between Rb and HBOC, and Rb and FAP. No differences were seen between PNDs for Rb and VHL and LFS.

In 14 out of 22 couples that used PND for Rb, one of the parents was a carrier of an RB1 mutation and the risk of a child with Rb was 50%. The other eight families concerned healthy parents with a child with a de novo RB1 mutation. The recurrence risk for these couples was 2–3%, based on possible germline mosaicism. For the other four cancer syndromes, the risk of an affected child was 50% for all cases. When confining the comparison analysis to Rb cases with a 50% risk, a significant difference in use of PND was still seen between Rb and FAP (p = 0.0011) and Rb and HBOC (p < 0.0001).

Number of PNDs per year

In Fig. 1 the number of PNDs for all hereditary cancer syndromes is plotted per year.

Fig. 1
Number of PNDs per hereditary cancer syndrome per year. Rb retinoblastoma, VHL Von Hippel–Lindau disease, LFS Li–Fraumeni syndrome, FAP familial adenomatous polyposis, HBOC hereditary breast and ovarian cancer, PND prenatal diagnosis

The first PND for Rb was done in 1993 and the last PND included in the study was done in 2013. In that period there has been neither a substantial increase nor decrease in the number of PNDs for Rb per year: the number of PNDs per year ranged from zero to four, with a mean of 1.5 per year. The number of PNDs per year for the other cancer syndromes varied between zero and three per syndrome per year. There was a trend towards more PNDs after 2009: eight out of a total of 28 PNDs for these cancer syndromes were performed between 2001 and 2009. The other 20 PNDs were performed between 2009 and 2013.

In 22 out of 35 PNDs for Rb, the pre-PND counselling and the invasive procedure was done in VUMC, where the National Retinoblastoma Treatment Center is located and the remaining thirteen pre-PND counselling and invasive procedures were conducted elsewhere. Pre-PND counselling and invasive procedures for the other cancer syndromes were performed in all participating hospitals, except for one: in the National Cancer Institute no invasive PND procedure is available and those patients were referred to one of the other eight centres.

Time between start of DNA diagnostic testing and first PND

In Table 2 the year of gene identification, start of DNA diagnostic testing per cancer syndrome is depicted and number of years between the start of DNA diagnostic testing and the year of the first PND. For Rb the first PND was done 3 years after DNA diagnostic testing had been introduced in the Netherlands. For the other cancer syndromes the first PND was performed between 10 and 15 years after DNA diagnosis became available.

Table 2
Genes related to the five hereditary cancer syndromes with year of gene identification, year of start of DNA diagnostic testing in the Netherlands, year the first prenatal diagnosis (PND) was performed, and the number of years between the start of testing ...

Analysis comparing uptake for Rb to FAP and HBOC was done again, while taking into account the year DNA diagnostic testing became available. Since DNA testing for FAP has been possible since 1991 and the first PND for Rb was performed in 1993, the analysis was unchanged for FAP. For HBOC, uptake of PND for Rb remained significantly different both for all PNDs (p value <2.2e−16) and when just comparing PNDs of couples with a 50% risk of a child with Rb (p value <1.296e−12).

Discussion

In this study, relatively large differences in the use of PND between cancer-predisposing syndromes were found. A significantly higher uptake for Rb than for the adult-onset cancer syndromes HBOC and FAP was seen. Uptake of PND did not differ significantly between Rb and two other early onset cancer syndromes VHL and LFS. PND for Rb started just 3 years after DNA diagnostic testing was introduced and uptake has been relatively stable over the years. PND for VHL, LFS, FAP and HBOC started 10–15 years after DNA testing was offered. A trend towards more PNDs for these syndromes after 2009 was noted.

The differences in uptake for PND observed in this study may be explained by several interdependent factors: differences in age of onset of cancer, disease penetrance, risk-reducing options and perceived disease burden, as noted by several authors in papers on PND or PGD for hereditary cancer [1, 5].

When age of onset is in adulthood, prospective parents may have the hope for better treatment options in the future for a carrier child, whereas in the case of childhood-onset the parents’ concerns will be more immediate. For adult-onset cancers, cancer diagnosis of an individual may not have been made until after family planning was completed. Rb is a high-penetrance disease of early childhood, and therefore parents of an affected child with Rb may still be in the reproductive age at the time of diagnosis of an affected child and may opt for PND in a subsequent pregnancy. Furthermore, physicians caring for patients with childhood cancer often have more intense contact with the family and will have more awareness of a possible impact of childhood cancer on family planning and be more knowledgeable about reproductive options than physicians caring for adult-onset cancer patients [31]. Differences in uptake may also be explained by the lack of risk-reducing options for the hereditary cancer syndromes with childhood-onset, apart from screening to detect cancer at an early stage [32]. For HBOC, mastectomy and prophylactic salpingo-oophorectomy can reduce cancer risk substantially, and the same applies to colectomy for FAP.

Lastly, previous studies on reproduction and hereditary cancer considered the perceived disease burden by individuals opting for assisted reproduction a factor of influence on uptake of PND [5]. For Rb, an extra disease burden follows from the intensive ophthalmological screening, which is done under anaesthesia in the first few years of life. Also, patients with heritable retinoblastoma have an increased risk for both a pineoblastoma (also referred to as trilateral retinoblastoma) and second tumours later in life and many have considerable late effects of treatment, e.g. an impaired vision or hearing loss. Perceived disease burden is not always related to a personal history of cancer, but can be shaped by many aspects, like caring for a family member with cancer [1, 5]. In our study of reproductive behaviour of individuals at risk of a child with Rb, the most important factor of influence on reproductive behaviour was perceived risk, not objective risk [4]. Both perceived disease burden and perceived risk may therefore be part of the reason eight parents with a child with a de novo RB1 mutation opted for PND, in spite of a recurrence risk of <3%.

One of the possible reasons PND uptake for Rb may differ from uptake for FAP and HBOC could be that diagnostic DNA testing of Rb started earlier than for FAP and HBOC. Therefore analysis comparing uptake for Rb to FAP and HBOC was done again, while taking into account the year DNA diagnostic testing became available. This analysis, however, did still show a significant difference between uptake for Rb and uptake for FAP and HBOC.

Observed higher uptake for Rb may be a reflection of differences in counselling between our clinical genetics department (with the highest number of counselees for Rb) and the other eight clinical genetics departments in the Netherlands. However, 13 PNDs and pre-PND counselling for Rb were performed elsewhere. Since there is a close collaboration between the nine clinical genetics departments in the Netherlands, policy towards counselling and PND is much the same.

PND for hereditary cancer has been reported in other countries, although in limited numbers, making it difficult to compare these data to our findings [1]. One paper on the clinical perspective on ethical arguments around PND and PGD for later-onset cancer syndromes from the Regional Genetics Service in Manchester mentioned one couple out of 110 families with FAP known in their centre that had undergone PND, and none from 356 HBOC families [14]. In Canada, PND for Rb is done to enhance early management of RB1 carrier infants and not with the option to terminate the pregnancy [33]. In the case of an affected child, premature delivery at 36 weeks’ gestation is recommended to be able to treat as early as possible.

One of the limitations of this study is that the number of mutation carriers in the reproductive age in each family was not known. However, since a relatively large number of families were included, we believe that our data on the uptake of PND in the cancer syndromes are by and large reliable. A trend towards increasing uptake of PND for hereditary cancers other than Rb after 2009 was observed. In 2008, PGD for hereditary cancer was temporarily restricted by the Dutch Minister of Health because of an ethical debate on PGD for diseases with a penetrance of <100%, such as HBOC [34]. Public debate in the media about hereditary cancer and reproduction during those years may have alerted at-risk couples to the option of both PGD and PND. Future research will have to determine whether the observed increasing trend of PND uptake over the past 5 years for VHL, LFS, FAP and HBOC will continue.

In conclusion, PND for Rb started many years before it was used for the other hereditary cancer syndromes. PND has been done significantly more often for Rb than for FAP and HBOC. Uptake of PND was not significantly different between Rb and VHL, and Rb and LFS. Early onset, high penetrance, lack of preventive surgery and perceived burden of disease may explain these differences. Knowledge regarding the underlying motives of couples that have opted for PND as a reproductive option is useful to improve care for families with a genetic predisposition for cancer.

Compliance with ethical standards

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

1. Offit K, Kohut K, Clagett B, et al. Cancer genetic testing and assisted reproduction. J Clin Oncol. 2006;24:4775–4782. doi: 10.1200/JCO.2006.06.6100. [PubMed] [Cross Ref]
2. Lammens C, Bleiker E, Aaronson N, et al. Attitude towards pre-implantation genetic diagnosis for hereditary cancer. Fam Cancer. 2009;8:457–464. doi: 10.1007/s10689-009-9265-5. [PMC free article] [PubMed] [Cross Ref]
3. Dommering CJ, van den Heuvel MR, Moll AC, et al. Reproductive decision-making: a qualitative study among couples at increased risk of having a child with retinoblastoma. Clin Genet. 2010;78:334–341. doi: 10.1111/j.1399-0004.2010.01484.x. [PubMed] [Cross Ref]
4. Dommering CJ, Garvelink MM, Moll AC, et al. Reproductive behavior of individuals with increased risk of having a child with retinoblastoma. Clin Genet. 2012;81:216–223. doi: 10.1111/j.1399-0004.2011.01791.x. [PubMed] [Cross Ref]
5. Rich TA, Liu M, Etzel CJ, et al. Comparison of attitudes regarding preimplantation genetic diagnosis among patients with hereditary cancer syndromes. Fam Cancer. 2014;13:291–299. doi: 10.1007/s10689-013-9685-0. [PMC free article] [PubMed] [Cross Ref]
6. Mitchell C, Nicolaides K, Kingston J, et al. Prenatal exclusion of hereditary retinoblastoma. Lancet. 1988;1:826. doi: 10.1016/S0140-6736(88)91687-X. [PubMed] [Cross Ref]
7. Ao A, Wells D, Handyside AH, et al. Preimplantation genetic diagnosis of inherited cancer: familial adenomatous polyposis coli. J Assist Reprod Genet. 1998;15:140–144. doi: 10.1023/A:1023008921386. [PMC free article] [PubMed] [Cross Ref]
8. Harper JC, Geraedts J, Borry P, et al. Current issues in medically assisted reproduction and genetics in Europe: research, clinical practice, ethics, legal issues and policy. European Society of Human Genetics and European Society of Human Reproduction and Embryology. Eur J Hum Genet. 2013;21(Suppl 2):S1–21. doi: 10.1038/ejhg.2013.219. [PMC free article] [PubMed] [Cross Ref]
9. Council of Europe. Background document on preimplantation and prenatal genetic testing. http://www.coe.int/t/dg3/healthbioethic/Activities/07_Human_genetics_en/default_en.asp. Updated 7-5-2015. Accessed 28-3-2016
10. Quinn GP, Vadaparampil ST, Bower B, et al. Decisions and ethical issues among BRCA carriers and the use of preimplantation genetic diagnosis. Minerva Med. 2009;100:371–383. [PubMed]
11. Wang CW, Hui EC. Ethical, legal and social implications of prenatal and preimplantation genetic testing for cancer susceptibility. Reprod Biomed Online. 2009;19(Suppl 2):23–33. doi: 10.1016/S1472-6483(10)60274-X. [PubMed] [Cross Ref]
12. Niermeijer MF, de Wert G, Dondorp W. Preimplantation genetic diagnosis for cancer. Lancet Oncol. 2006;7:794–795. doi: 10.1016/S1470-2045(06)70876-2. [PubMed] [Cross Ref]
13. Niermeijer MF, de Die-Smulders CE, Page-Christiaens GC, de Wert GM. Genetic cancer syndromes and reproductive choice: dialogue between parents and politicians on preimplantation genetic diagnosis. Ned Tijdschr Geneeskd. 2008;152:1503–1506. [PubMed]
14. Clancy T. A clinical perspective on ethical arguments around prenatal diagnosis and preimplantation genetic diagnosis for later onset inherited cancer predispositions. Fam Cancer. 2010;9:9–14. doi: 10.1007/s10689-009-9271-7. [PubMed] [Cross Ref]
15. Leading edge Ethics of preimplantation genetic diagnosis for cancer. Lancet Oncol. 2006;7:611. doi: 10.1016/S1470-2045(06)70768-9. [PubMed] [Cross Ref]
16. van Oostrom I, Meijers-Heijboer H, Lodder LN, et al. Long-term psychological impact of carrying a BRCA1/2 mutation and prophylactic surgery: a 5-year follow-up study. J Clin Oncol. 2003;21:3867–3874. doi: 10.1200/JCO.2003.10.100. [PubMed] [Cross Ref]
17. Ethics Committee of American Society for Reproductive Medicine Use of preimplantation genetic diagnosis for serious adult onset conditions: a committee opinion. Fertil Steril. 2013;100:54–57. doi: 10.1016/j.fertnstert.2013.02.043. [PubMed] [Cross Ref]
18. Cobben JM, Brocker-Vriends AH, Leschot NJ. Prenatal diagnosis for hereditary predisposition to mammary and ovarian carcinoma—defining a position. Ned Tijdschr Geneeskd. 2002;146:1461–1465. [PubMed]
19. Lodder LN, Frets PG, Trijsburg RW, et al. Attitudes towards termination of pregnancy in subjects who underwent presymptomatic testing for the BRCA1/BRCA2 gene mutation in The Netherlands. J Med Genet. 2000;37:883–884. doi: 10.1136/jmg.37.11.883. [PMC free article] [PubMed] [Cross Ref]
20. Lohmann DR, Gallie BL (2015) Retinoblastoma. http://www.ncbi.nlm.nih.gov/books/NBK1452/. Updated 19-11-2015. Accessed 22-4-2016
21. Frantzen C, Links TP, Giles RH (2015) Von Hippel–Lindau Disease. http://www.ncbi.nlm.nih.gov/books/NBK1463/. Updated 6-8-2015. Accessed 14-3-2016
22. Schneider K, Zelley K, Nichols KE, Garber J (2013) Li–Fraumeni syndrome. http://www.ncbi.nlm.nih.gov/books/NBK1311/. Updated 11-4-2013. Accessed 17-12-2015
23. Jasperson KW, Burt RW (2014) APC-associated polyposis conditions. http://www.ncbi.nlm.nih.gov/books/NBK1345/. Updated 27-3-2014. Accessed 17-4-2016
24. Petrucelli N, Daly MB, Feldman GL (2013) BRCA1 and BRCA2 hereditary breast and ovarian cancer. http://www.ncbi.nlm.nih.gov/books/NBK1247/. Updated 26-9-2013. Accessed 17-11-2015
25. Friend SH, Bernards R, Rogelj S, et al. A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature. 1986;323:643–646. doi: 10.1038/323643a0. [PubMed] [Cross Ref]
26. Latif F, Tory K, Gnarra J, et al. Identification of the von Hippel–Lindau disease tumor suppressor gene. Science. 1993;260:1317–1320. doi: 10.1126/science.8493574. [PubMed] [Cross Ref]
27. Malkin D, Li FP, Strong LC, et al. Germ line p53 mutations in a familial syndrome of breast cancer, sarcomas, and other neoplasms. Science. 1990;250:1233–1238. doi: 10.1126/science.1978757. [PubMed] [Cross Ref]
28. Kinzler KW, Nilbert MC, Su LK, et al. Identification of FAP locus genes from chromosome 5q21. Science. 1991;253:661–665. doi: 10.1126/science.1651562. [PubMed] [Cross Ref]
29. Miki Y, Swensen J, Shattuck-Eidens D, et al. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science. 1994;266:66–71. doi: 10.1126/science.7545954. [PubMed] [Cross Ref]
30. Wooster R, Bignell G, Lancaster J, et al. Identification of the breast cancer susceptibility gene BRCA2. Nature. 1995;378:789–792. doi: 10.1038/378789a0. [PubMed] [Cross Ref]
31. Brandt AC, Tschirgi ML, Ready KJ, et al. Knowledge, attitudes, and clinical experience of physicians regarding preimplantation genetic diagnosis for hereditary cancer predisposition syndromes. Fam Cancer. 2010;9:479–487. doi: 10.1007/s10689-010-9343-8. [PubMed] [Cross Ref]
32. Julian-Reynier C, Chabal F, Frebourg T, et al. Professionals assess the acceptability of preimplantation genetic diagnosis and prenatal diagnosis for managing inherited predisposition to cancer. J Clin Oncol. 2009;27:4475–4480. doi: 10.1200/JCO.2008.21.2712. [PubMed] [Cross Ref]
33. Gallie B. Canadian guidelines for retinoblastoma care. Can J Ophthalmol. 2009;44:639–642. doi: 10.3129/i09-229. [PubMed] [Cross Ref]
34. Sheldon T. Netherlands debates screening for breast cancer. BMJ. 2008;336:1270. doi: 10.1136/bmj.39598.672500.DB. [PMC free article] [PubMed] [Cross Ref]

Articles from Springer Open Choice are provided here courtesy of Springer