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Stem Cell Reports. 2017 March 14; 8(3): 589–604.
Published online 2017 February 9. doi:  10.1016/j.stemcr.2016.12.026
PMCID: PMC5355564

GATA6 Plays an Important Role in the Induction of Human Definitive Endoderm, Development of the Pancreas, and Functionality of Pancreatic β Cells

Summary

Induced pluripotent stem cells were created from a pancreas agenesis patient with a mutation in GATA6. Using genome-editing technology, additional stem cell lines with mutations in both GATA6 alleles were generated and demonstrated a severe block in definitive endoderm induction, which could be rescued by re-expression of several different GATA family members. Using the endodermal progenitor stem cell culture system to bypass the developmental block at the endoderm stage, cell lines with mutations in one or both GATA6 alleles could be differentiated into β-like cells but with reduced efficiency. Use of suboptimal doses of retinoic acid during pancreas specification revealed a more severe phenotype, more closely mimicking the patient’s disease. GATA6 mutant β-like cells fail to secrete insulin upon glucose stimulation and demonstrate defective insulin processing. These data show that GATA6 plays a critical role in endoderm and pancreas specification and β-like cell functionality in humans.

Keywords: GATA6, stem cells, pancreatic agenesis, definitive endoderm, β cell, retinoic acid

Introduction

Pancreatic agenesis is a rare congenital disease caused by a mutation in PDX1 (Stoffers et al., 1997), GATA4 (Shaw-Smith et al., 2014), or most commonly GATA6 (Chao et al., 2015, De Franco et al., 2013, Lango Allen et al., 2012, Stanescu et al., 2014). The majority of GATA6 mutations leading to pancreatic agenesis are de novo heterozygous mutations. Some GATA6 mutations have incomplete penetrance as determined by patients having identical mutations to pancreatic agenesis patients, but displaying either adult-onset diabetes or an absence of pancreatic abnormalities (Bonnefond et al., 2012, De Franco et al., 2013). The majority of pancreatic agenesis patients also display a combination of other defects including congenital heart defects, gut abnormalities, and intrauterine growth retardation (Chao et al., 2015).

GATA6 belongs to a six-member family of transcription factors that bind to the consensus sequence (A/T)GATA(A/G). GATA1, GATA2, and GATA3 are mainly expressed in hematopoietic cell lineages, while GATA4, GATA5, and GATA6 are predominantly expressed in the heart, gonads, and endodermal-derived tissues (Viger et al., 2008). GATA6 is known to regulate endodermal gene expression and development of endoderm-derived organs (Molkentin, 2000). In mice, GATA6 is expressed in the primitive streak, heart, lung, intestine, gonads, adrenal, and pancreatic tissues (Koutsourakis et al., 1999, Liu et al., 2002). Within the adult pancreatic tissue, GATA6 is expressed in both the exocrine tissue and the islets of Langerhans (Sartori et al., 2014).

In contrast to the severe disease phenotype found in humans with GATA6 heterozygous mutations, GATA6 heterozygous mice are fertile and phenotypically normal. Homozygous GATA6 null mice are embryonic lethal (Morrisey et al., 1998). Using tetraploid complementation, GATA6 has been shown to be essential for extra-embryonic endoderm development explaining the embryonic lethality (Koutsourakis et al., 1999, Zhao et al., 2005); however, GATA6 null cells can contribute to the definitive endoderm. Analysis of a loss of GATA6 in pancreas progenitors or adult β cells has demonstrated minimal impact on endocrine function, with normal numbers of β cells and no overt signs of diabetes despite a mild impact on endoplasmic reticulum stress (Carrasco et al., 2012, Martinelli et al., 2013, Sartori et al., 2014, Xuan et al., 2012).

Due to the major differences in phenotype between human and murine GATA6 disease models, human pluripotent stem cells (PSCs) offer an alternative system for the in vitro study of GATA6. With recent developments in the genome-editing field, the use of clustered regularly interspaced short palindromic repeats (CRIPSR)/CAS9 technology (Ran et al., 2013) has enabled PSCs to become an even more powerful model system as mutant and control isogenic lines can be made to avoid confounding results due to differing genetic backgrounds.

Here, we study GATA6 mutant human PSCs. Induced pluripotent stem (IPS) cells were generated from a previously described pancreatic agenesis patient having a heterozygous GATA6 mutation (Stanescu et al., 2014). Using genome editing, PSC lines with mutations in both alleles of GATA6 were generated and failed to differentiate into definitive endoderm due to a block at the primitive streak stage of development. Re-expression of GATA6 or other GATA family members restored this defect. Using endodermal progenitor (EP) cells as a tool to bypass the endoderm defect, pancreatic β cell differentiation was examined. We found that all mutant lines maintained the ability to differentiate into pancreatic β-like cells but that these cells were functionally defective in glucose responsiveness. Finally, we show that limiting retinoic acid (RA) signaling during pancreas induction in the GATA6 mutant lines led to a dramatic decrease in pancreas specification and β cell generation. These data suggest that human GATA6 plays a critical role in endoderm development and functionality of pancreatic β-like cells.

Results

Establishment of GATA6 PSC Lines

To study the role of GATA6 in human development, mutant and control PSC lines were generated by standard reprogramming and CRISPR/Cas genome editing. An iPS cell line was generated from cells of a previously described patient expressing a heterozygous GATA6 mutation (Stanescu et al., 2014). The 4 base pair (bp) duplication in the second exon of GATA6 causes a frameshift mutation resulting in a truncated protein (Figures 1A and 1B). This patient-derived iPS cell line, is designated IPS+/indel (Table S1). To generate cell lines expressing mutations in both alleles of GATA6, CRISPR/Cas9 genome editing was performed using the IPS+/indel cell line and the Mel1-INS-GFP (Micallef et al., 2012) embryonic stem (ES) cell line (Figure 1C). The IPS+/indel cell line was used to maintain genetic background identity to the patient’s cells for experimental comparison. The Mel1-INS-GFP ES cell line (designated ES+/+, Table S1) was used for two reasons in addition to confirming phenotypes in a second genetic background. First, the Mel1-INS-GFP line allows easy purification of β-like cells as it contains a GFP reporter in the insulin locus. Second, to assist with inducible gene expression studies, we generated a Mel1-INS-GFP sub-line that constitutively expressed the reverse tet transactivator (rtTA) targeted to the AAVS1 safe harbor locus (Figures S1A and S1B) using a previously described methodology (Hockemeyer et al., 2009, Tiyaboonchai et al., 2014). For CRISPR/Cas genome editing, the guide RNA (gRNA) was designed to target GATA6 near the patient mutation site (Figure 1B) creating frameshift insertion and/or deletion (INDEL) mutations in both alleles of GATA6 (Table S1 and Figure S1C). The genome-edited patient iPS cell line is designated IPSindel/indel and the genome-edited Mel-INS-GFP line is designated ESindel/indel (Table S1). To generate an isogenic control for the IPS+/indel line, genome editing was used to correct the mutation, and this line is designated IPS+/+ (Table S1). The IPS+/indel line was confirmed for pluripotency (Figure S2) and all genome-edited PSC lines were confirmed to have a normal karyotype (Figure S1D).

Figure 1
Generation of PSC Lines with GATA6 Mutations

As GATA6 is expressed in the primitive streak and not in PSCs, protein and transcript levels were examined in control and mutant cells utilizing a protocol that induces definitive endoderm (Cheng et al., 2012, D’Amour et al., 2005). Using western blot analysis, full-length GATA6 protein was detected in the control lines, ES+/+, and IPS+/+, and in the patient line, IPS+/indel, that expressed one normal allele of GATA6 (Figure 1C). While the GATA6 transcript contains two alternative start sites (Brewer et al., 1999), we predominantly observe the smaller isoform. The patient line, IPS+/indel, also expressed one mutant allele of GATA6 that generated a truncated GATA6 protein of ~35 kDa, the only form of GATA6 detected in the compound heterozygous mutant lines, IPSindel/indel and ESindel/indel (Figure 1C). Using flow cytometry, GATA6 protein was also quantified by mean fluorescence intensity (MFI). We found that the truncated GATA6 protein in the IPSindel/indel line was expressed at significantly lower levels (0.27 ± 0.06) relative to the IPS+/indel (0.97 ± 0.13) and IPS+/+ (normalized to 1) lines (Figure 1D). The same results were observed in the ESindel/indel line, with significantly decreased levels of GATA6 (0.30 ± 0.01-fold) compared with the ES+/+ line (Figure S3D).

In addition to GATA6, GATA4 is another member of the GATA family that is upregulated during primitive streak and definitive endoderm induction (Arceci et al., 1993, Czysz et al., 2015). Both GATA4 and GATA6 transcript levels were measured by qPCR in definitive endoderm cells. GATA6 levels were significantly decreased in the IPSindel/indel cell line compared with the IPS+/+ cell line (Figure 1E), suggesting that GATA6 may be part of a self-regulatory feedback loop. GATA4 was decreased in a dose-dependent manner in the IPS+/indel and IPSindel/indel lines (Figure 1F). A time-course analysis of both transcripts during endoderm differentiation in IPS+/+ cells showed that GATA6 is expressed at higher levels and more rapidly than GATA4 (Figure 1G). Together, these data suggest cross-talk between GATA6 and GATA4, and GATA6 may be maintaining expression of both itself and GATA4 during endoderm induction.

Definitive Endoderm Differentiation

To analyze the impact of GATA6 on differentiation to definitive endoderm, a time-course analysis was performed using a modification of established protocols (D’Amour et al., 2005, Kubo et al., 2004). The expression of developmentally regulated markers at different stages of differentiation was analyzed using the IPS+/+, IPS+/indel, and IPSinde/indel lines (Figure 2A). These experiments were also repeated in the ES+/+ and ESindel/indel lines (Figure S3). In all lines, the pluripotency markers NANOG, SOX2, and OCT4 were downregulated by day 2 of differentiation, and the primitive streak markers brachyury (T), goosecoid (GSC), and eomesodermin (EOMES) were expressed at the appropriate times. The definitive endoderm markers SOX17, FOXA2, and HNF1B failed to upregulate in the IPSindel/indel and ESindel/indel cell lines compared with the IPS+/indel, IPS+/+, and ES+/+ lines (Figures 2A and S3A). By using intracellular flow cytometry to examine the co-expression of SOX17 and FOXA1, these data were confirmed and quantitated (Figures 2B, 2C, S3B, and S3C). Robust co-expression of SOX17 and FOXA1 was observed in IPS+/+ (82% ± 4%), ES+/+ (82% ± 7%) and IPS+/indel (75% ± 3%) lines. Compared with their respective control cells, there was a ~27-fold decrease in the IPSindel/indel (2.7% ± 0.7%) and a ~6-fold decrease in the ESindel/indel (14% ± 3%) lines. These data were confirmed in all cell lines by immunofluorescence staining for SOX17 and FOXA2 (Figures 2D and S3E). These data show that GATA6 is a critical transcription factor required during definitive endoderm specification in human cells.

Figure 2
GATA6 Is Required for Definitive Endoderm Differentiation of PSC Lines

To determine if GATA6 affected differentiation to the other two germ layers, established protocols were used to monitor differentiation of ES+/+, IPS+/indel, ESindel/indel, and IPSindel/indel lines. All cell lines displayed similar differentiation efficiency to the mesoderm and ectoderm germ layers suggesting that GATA6 is not required (Figures S3H and S3I).

Rescue of Definitive Endoderm by GATA6

To confirm that GATA6 was responsible for the decrease in endoderm induction from the ESindel/indel line, GATA6 rescue experiments were performed. Because the ESindel/indel line constitutively expresses rtTA, a lentiviral vector containing the tet response element was used to express GATA6/RFP in a doxycycline (Dox)-inducible manner (Figure 3A). Adding Dox on day 1 of differentiation, robust co-expression of SOX17 and FOXA1 was observed in the ESindel/indel line only after rescue with full-length and not truncated GATA6 (Figures 3B and 3C). Compared with the empty vector which only expresses RFP, overexpression of the truncated GATA6 protein resulted in a decreased ESindel/indel cell differentiation to definitive endoderm, suggesting a possible dominant-negative activity of the truncated protein (Figures 3B and 3C). Gene expression analysis of other endodermal markers was also performed in cells expressing the GATA6 and truncated GATA6 transgenes by sorting the RFP-positive cells at day 5 of differentiation. The expression levels of SOX17, FOXA2, and HNF1B in GATA6-rescued ESindel/indel cells were comparable with normal levels in ES+/+ cells differentiated to definitive endoderm (Figure 3E). Expression levels of these markers in ESindel/indel cells expressing truncated GATA6 were not statistically different to cells expressing the empty vector.

Figure 3
Rescue of Definitive Endoderm Differentiation

To address a temporal requirement of GATA6 during definitive endoderm specification, Dox was added at either the primitive streak (days 0–1) or the endoderm specification (days 2–3) stage of development (Figure 2A). RFP+ cells were analyzed for the co-expression of SOX17 and FOXA1 at day 5 of differentiation (Figure 3D). The addition of Dox at days 0 and 1 resulted in 77% ± 4.5% and 82% ± 4.2% of SOX17+ and FOXA1+ cells while the addition of Dox at days 2 and 3 resulted in 50% ± 5.9% and 30% ± 4.0% in SOX17+ and FOXA1+ cells. These data show that robust endoderm specification requires GATA6 expression at the primitive streak stage.

Rescue of Definitive Endoderm by Other GATA Family Members

All members of the GATA transcription factor family share conserved DNA activation and zinc finger DNA-binding domains (Molkentin, 2000, Viger et al., 2008). To determine if other GATA family members can compensate for loss of GATA6, the same rescue experiments described above were performed using lentiviral vectors expressing GATA1, 3, and 4. GATA4 was chosen because it is functionally redundant with GATA6 in animal models (Holtzinger and Evans, 2005, Xuan et al., 2012). GATA1 and GATA3 are not typically expressed in definitive endoderm and were initially chosen as controls. Rescue with GATA1, GATA3, and GATA4 resulted in cells co-expressing 53% ± 5.4%, 70% ± 3.6%, and 76% ± 3.3% SOX17 and FOXA1, respectively. The rescue of definitive endoderm differentiation with GATA4 was expected, but the ability of GATA1 and GATA3 to rescue definitive endoderm was not and suggests that any GATA family member, even those not typically expressed in endoderm, may compensate for GATA6. These data were confirmed by analysis of SOX17, FOXA2, and HNF1B on RFP+ sorted cells (Figure 3E). As mutant ESindel/indel cells have significantly lower GATA4 expression in definitive endoderm compared with control ES+/+ cells (Figure 1F), we questioned whether other GATA family members were rescuing the differentiation by inducing GATA4. We show that any of the GATA factors can rescue expression of endogenous GATA4 (Figure 3E), although further experimentation would be needed to formally demonstrate that the rescue was simply due to GATA4 induction.

GATA6 Mutants Undergo Increased Apoptosis during Endoderm Induction that Can Be Rescued by Growth Factor Supplementation

During endoderm induction, a decrease in cell number at day 2 of differentiation was observed with the iPSindel/indel and ESindel/indel cell lines compared with the iPS+/+ and ES+/+ cell lines (Figures 3F and S4A). This time point directly follows primitive streak induction and is when GATA6 would begin to be expressed (Figure 1G). We hypothesized that in GATA6 mutants, cells attempting to commit to endoderm are lost. To determine whether apoptosis caused the decrease in cell number, TUNEL staining was performed on day 2 of differentiation. We found a 2.7- to 4.7-fold increase in the percentage of TUNEL-positive cells in the iPSindel/indel and ESindel/indel cell lines compared with the iPS+/+ and ES+/+ cell lines (Figures 3G and S4B) suggesting that cell death may be a factor contributing to the decreased cell numbers during definitive endoderm specification.

To determine whether a rescue of apoptosis would also rescue the defect in definitive endoderm differentiation of iPSindel/indel and ESindel/indel cells, increasing concentrations of the pro-survival growth factor basic fibroblast growth factor (bFGF) were used to treat the cells for 24 hr at day 1 of differentiation, prior to the observed decrease in cell number. Co-expression of FOXA1 and SOX17 was examined at the end of the definitive endoderm differentiation. While the efficiency of differentiation in the IPS+/+ and ES+/+ cell lines was unaffected by higher bFGF concentrations (Figures S4D and S4E), there was a partial rescue of the definitive endoderm differentiation in both the ESindel/indel and iPSindel/indel cell lines (Figures 3H and S4C), suggesting a cell survival defect in the ESindel/indel and iPSindel/indel cells.

β-like Cell Differentiation of GATA6 Mutants from Endodermal Progenitor Cells

The use of established differentiation protocols to study the role of GATA6 in pancreas development (Pagliuca et al., 2014, Rezania et al., 2014, Rezania et al., 2012, Russ et al., 2015) was difficult due to the inability of the ESindel/indel and IPSindel/indel cell lines to efficiently form definitive endoderm. To overcome this developmental block, we established EP cells from the IPS cell allelic series as well as the ES+/+ and ESindel/indel cell lines (Cheng et al., 2012). EP cells express GATA6 at lower levels but GATA4 and GATA3 at higher levels compared with definitive endoderm, making the establishment of these lines feasible (Cheng et al., 2012). Cells were differentiated to definitive endoderm and CXCR+ CKIT+ cells were sorted for the generation of EP cell lines. All lines were characterized as EP cells displaying self-renewal capacity to greater than 20 passages (data not shown) and expression of appropriate markers including SOX17, FOXA1, FOXA2, EOMES, TBX3, MSX2, MEIS2, and ID2 (Figure S5).

To study pancreatic cell fate, the EP cell lines were differentiated into β-like cells following our published protocol (Cheng et al., 2012). All EP cell lines generated β-like cells co-expressing C-peptide and PDX1 (Figure 4A). At the end stage of the adherent differentiation, cells were aggregated into suspension culture by dispase treatment to enrich for C-peptide+ cells. Following the aggregation, the percentage of C-peptide+ cells was comparable between all lines in both genetic backgrounds (Figure 4B). The efficiency of differentiation, as calculated by absolute yield of C-peptide+ cells per cell seeded relative to the respective controls of each genetic background, was found to be significantly lower in IPS+/indel, IPSindel/indel, and ESindel/indel cells (Figure 4C). Thus, while mutant GATA6 EP cells can be differentiated into β-like cells, the efficiency of differentiation is lower compared with wild-type cells of the same genetic background.

Figure 4
GATA6 Is Dispensable for Differentiation of Pancreatic β-like Cells from PSCs

Retinoic Acid as a Modulator of the Phenotype of Mutant GATA6 Cells

The patient from whom the IPS+/indel cells were derived was born with pancreas agenesis. The ability to generate β-like cells from the GATA6 mutant lines in vitro was unexpected, and we hypothesized that our in vitro differentiation system may act to bypass the developmental defect. The differentiation protocol supplies various extrinsic signals necessary to drive pancreas specification and maturation into endocrine cells. Therefore, if GATA6 and/or GATA4 act in vivo to modulate, or are the target of one of these signals, it could explain the differences in phenotype between in vitro and in vivo development. Furthermore, if our hypothesis is correct, we predict that using limiting doses of these inductive signals might reveal a more severe phenotype in the GATA6 mutant cell lines. We decided to focus our further studies on the pancreas induction stage from foregut endoderm, as defects here would be predicted to lead to pancreas agenesis. Virtually all differentiation protocols from either ES or EP cells utilize similar inductive signals at this stage including FGF, inhibition of sonic hedgehog, and RA signaling (Cheng et al., 2012, Kroon et al., 2008, Nostro et al., 2011, Rezania et al., 2013).

To determine whether loss of GATA6 affects pancreatic specification, induction of PDX1, a master regulator of pancreas development, was examined (Pan and Wright, 2011). Under standard differentiation conditions, IPS+/+ EP cells more efficiently generate PDX1+ pancreatic progenitor cells (Figures 5A and 5D) compared with IPS+/indel (Figures 5A and 5E) and IPSindel/indel EP cells (Figure 5F). The mutant cells still generate a significant proportion of PDX1+ cells. To address whether high levels of exogenous signals provided in vitro allow GATA6 mutant cells to more efficiently induce PDX1, limiting concentrations of FGF10, cyclopamine, and RA were tested during the differentiation in pilot studies. While lowering the concentrations of FGF10 or cyclopamine did not have a major impact on PDX1 induction (data not shown), lowering RA concentrations by 80-fold to 0.025 μM resulted in a statistically significant decrease in the percentage of PDX1+ cells generated in the IPS+/indel and IPSindel/indel cell lines (Figures 5E and 5F). In contrast, the percentage of PDX1+ cells induced in the IPS+/+ cell line was not significantly affected by the lower dose of RA, while the MFI of PDX1 was somewhat decreased (Figures 5B, 5D, and 5G). Adding no RA resulted in an even further decrease in PDX1 in the iPS+/indel and iPSindel/indel cells though IPS+/+ cells were also affected (Figures 5A–5G). These experiments were repeated in ES+/+ and ESindel/indel cells with similar findings (Figures S6A–S6D). Together, these data suggest that the addition of excess RA during in vitro differentiation may allow GATA6 mutant cells to form pancreatic progenitors and subsequently β-like cells.

Figure 5
Retinoic Acid Acts as a Modulator of the GATA6 Phenotype

Previous findings have shown that GATA4 and GATA6 expression can be induced by RA (Arceci et al., 1993, Mauney et al., 2010). GATA4 RNA and GATA6 protein levels were examined during the differentiation of IPS+/+ cells with decreasing amounts of RA. A dose-dependent decrease in both GATA4 and GATA6 was observed with decreased RA concentrations (Figures 5H–5J). Analysis of GATA4 in GATA6 mutant lines also demonstrated a decrease in expression that was exacerbated with lower RA. These results were confirmed in the ES cell background (Figures S6D–S6F). These observations suggest that GATA4 is unable to compensate for loss of GATA6. Instead, GATA4 is further downregulated by the loss of GATA6 and low-dose RA signaling. These findings offer a possible explanation for why GATA6 mutant mouse models do not mimic the pancreas agenesis phenotype in humans as multiple organs examined in GATA6 null mice did not display decreases in GATA4 (Walker et al., 2014, Zhao et al., 2005).

To determine the effects of RA dosage on β cell development, the EP cell lines were differentiated to β-like cells under different doses of RA during pancreatic induction. We examined the yield of C-peptide+ β-like cells from the various mutant and wild-type IPS-derived EP lines. Confirming results in Figure 4C, under standard differentiation conditions, the IPS+/indel and IPSindel/indel cell lines display a reduced yield of C-peptide+ cells (~60%–80% decreased), but in low RA conditions a more severe decrease in C-peptide+ cells is seen in both mutant lines (~80%–95% decreased) (Figure 5K). Similar results were seen in the ES+/+ and ESindel/indel cell lines (Figure S6H). These data confirm that differentiation of GATA6 mutant stem cells in the context of a low dose of RA can more closely mimic the patient phenotype with a severe loss of pancreatic β-like cells.

Gene Expression Analysis of GATA6 Mutant β-like Cells

To analyze gene expression differences due to loss of GATA6, INS-GFP+ β-like cells were sorted from the ES+/+ and ESindel/indel cell lines and a panel of genes important for β cell function and development were assayed (Figure 6A). Significant differences were found in PDX1, PCSK1, HK, GLUT1, GATA4, and HNF4α (Figure 6B).

Figure 6
Gene Expression and Functional Analysis of C-Peptide+ β-Like Cells

PDX1 was confirmed by flow cytometry to be significantly lower in iPSindel/indel and ESindel/indel cells compared with IPS+/+ and ES+/+ β-like cells. However, there was no difference in PDX1 in IPS+/indel cells compared with IPS+/+ β-like cells (Figures 6Ci, 6Cii, and S7A). By flow cytometry, HNF4α trended toward a dose-dependent decrease within both the IPS and ES cell allelic series (Figures 6Di, 6Dii, and S7B). PCSK1, a gene involved in proinsulin to insulin processing (Steiner, 2004) was significantly lower in the ESindel/indel cells compared with the ES+/+ cells, thus we hypothesize that there should be defective proinsulin processing and accumulation of proinsulin. Flow cytometry demonstrated a statistically significant, dose-dependent increase in proinsulin in the IPS and ES cell GATA6 allelic series (Figures 6Ei, 6Eii, and S7C). In both the IPS and ES cell backgrounds, there was a trend toward increased proinsulin compared with insulin secretion in the mutant compared with the wild-type β-like cells (Figure 6F). Thus, GATA6 may be involved in insulin processing in EP-derived β-like cells and may regulate other key genes involved in β cell function such as HNF4A and PDX1.

Functional Analysis of GATA6 Mutant β-like Cells

To analyze the functionality of EP differentiated β-like cells, their responsiveness to glucose stimulation was determined. Upon glucose stimulation, the increase in C-peptide secretion of the IPS+/+ EP-derived β-like cells was 2.1 ± 0.18-fold over basal levels (Figure 6G). The IPS+/indel and IPSindel/indel EP-derived β-like cells were unresponsive to glucose stimulation with stimulation indexes of 1.0 ± 0.14 and 1.2 ± 0.15, respectively. These results were corroborated in the ES cell background. Control ES+/+ EP-derived β-like cells had a stimulation index of 2.1 ± 0.22-fold, while mutant ESindel/indel β-like cells were unresponsive with a stimulation index of 1.0 ± 0.08-fold (Figure 6G). In unstimulated conditions, all of the β-like cell lines within both allelic series, secreted similar amounts of basal C-peptide when normalized for the absolute number of C-peptide+ cells per culture (Figure S7E). These data demonstrate that the levels of GATA6 are critical in determining the functional responsiveness of pancreatic β-like cells to glucose stimulation in an in vitro setting.

Discussion

This study established a human in vitro PSC model system to study the role of GATA6 in the development of the pancreas and the functionality of β-like cells. Considering that GATA6 null mice are embryonic lethal due to a requirement for extra-embryonic endoderm (Morrisey et al., 1998), PSCs are advantageous because the extra-embryonic endoderm is unnecessary for their maintenance and differentiation. We have shown that while IPS+/indel cells with one normal allele have no defects in definitive endoderm specification, ESindel/indel and IPSindel/indel cells with two defective alleles failed to efficiently differentiate into definitive endoderm. The downregulation of pluripotency markers and the induction of primitive streak markers remained normal, suggesting a defect in the transition of cells from the primitive streak stage of development to definitive endoderm. Transgene expression of GATA6 in the ESindel/indel cells rescued endoderm differentiation demonstrating the importance of GATA6 in definitive endoderm specification and that off-target effects of genome editing were not responsible for the observed phenotype.

In chimeric mouse models, GATA6 null cells can contribute to the formation of the primitive gut tube (Koutsourakis et al., 1999). The inability of human ESindel/indel and IPSindel/indel cells to generate definitive endoderm (Figures 2 and S3) suggest species-specific differences in the role of GATA6 during endoderm development. One explanation for this difference could be the timing of GATA6 induction in the human system. We observed that the expression of GATA6 slightly preceded GATA4 expression during endoderm induction from PSCs (Figure 1G). No other GATA factors were expressed at this time (data not shown). In mouse models, GATA4 and GATA6 are co-expressed in the primitive streak (Morrisey et al., 1997), therefore it is possible that in the murine system, GATA4 is compensating for GATA6, leading to the differences in phenotype.

In the mutant ESindel/indel and IPSindel/indel cell lines, there was decreased expression of both GATA6 (Figures 1E and S3F) and GATA4 (Figures 1F and S3G) during definitive endoderm differentiation, suggesting that GATA6 may regulate its own expression in addition to GATA4. A similar observation has been made in murine visceral and primitive endoderm of GATA6 knockout embryos (Morrisey et al., 1998). However, loss of GATA6 in the heart (Zhao et al., 2005) or the jejunum of embryos (Walker et al., 2014) did not lead to a decrease in GATA4 levels. These data support the possibility that GATA4 and GATA6 are differentially regulated in definitive endoderm and pancreatic lineages in human and mouse leading to the observed differences.

The defective definitive endoderm differentiation in both ESindel/indel and IPSindel/indel cells could be partially rescued by increased bFGF signaling (Figures 3H and S4C). During definitive endoderm differentiation, the decrease in cell number is transient, and it is possible that there may be cell proliferation of non-endodermal cell types that can fill the void left by loss of endodermal cells. Furthermore, there is a more severe deficiency in definitive endoderm differentiation of IPSindel/indel cells (Figure 2) than in ESindel/indel cells (Figure S3), suggesting genetic background influences the phenotype. In both genetic backgrounds, there is a consistent 2- to 3-fold increase of SOX17+/FOXA1+ endoderm with increasing amounts of bFGF, potentiating a partial rescue of the definitive endoderm phenotype and suggesting that apoptosis may only be a partial cause of the defect in endoderm specification with other mechanisms also playing a role.

Our data demonstrating that GATA6 mutant stem cell lines could generate both pancreatic progenitors and β-like cells (Figures 4 and and5),5), even with decreased efficiency, was somewhat surprising as patients with GATA6 mutations often have pancreas agenesis (Stanescu et al., 2014). We identified RA signaling as an exogenous signal that may overcome the developmental block of GATA6 loss in the in vitro differentiation system. Consistent with previous reports, a decrease in the expression of both GATA4 (Figures 5H and S6E) and GATA6 (Figures 5I and S6F) was observed when limiting amounts of RA were used during the differentiation of wild-type cells to pancreatic progenitors (Arceci et al., 1993, Mauney et al., 2010). Only when low doses of RA were used during the differentiation of indel/+ or indel/indel GATA6 mutant cell lines was a more significant loss of both pancreatic progenitors and β-like cells observed (up to 95% loss; Figures 5K and S6H).

Heterozygous GATA6 mutations have incomplete penetrance as displayed by different phenotypes in family members having identical mutations (Bonnefond et al., 2012). These differences in the agenesis phenotype could be partially explained by variability in the levels of endogenous RA signaling suggesting nutritional supplementation as a possible means to prevent pancreas agenesis by boosting RA signaling and regulating expression of GATA family members. We propose a model (Figure 7) to both explain our results as well as provide insights into the role that GATA6 may play during pancreas development in vivo. In wild-type cells, RA may induce the expression of GATA4 and GATA6, allowing levels to increase above the threshold required for the formation of pancreatic progenitors (Figure 7A). A decrease in endogenous RA signaling could decrease the expression of GATA4 and GATA6, but in the context of two normal GATA6 alleles, sufficient levels of both factors are produced, resulting in the formation of pancreatic progenitors (Figure 7B). Similarly, the loss of one allele of GATA6 in the context of high RA signaling leads to decreased GATA factor expression, but again GATA levels remain above a critical threshold for pancreatic progenitor formation (Figure 7C). Conversely, a combined loss of one allele of GATA6 with limiting RA signaling could potentiate lower levels of GATA4, resulting in overall GATA factors falling below a critical level that may cause pancreas agenesis (Figure 7D).

Figure 7
Model for the Incomplete Penetrance of GATA6 Heterozygous Mutations

The model systems described here are also important for studying the subset of diabetic patients without agenesis who have GATA6 mutations. These systems provide a means to dissect the underlying disease mechanism during development and to study the role of GATA6 in endoderm and pancreatic development. The utilization of an intermediate progenitor population was essential for defining the role of GATA6 in pancreatic β-cell functionality, however, with the caveat that this may affect the phenotypes that could be observed. A future interest will be examination of pancreatic β cell function in cells expressing heterozygous GATA6 mutations from patients who do not have pancreatic agenesis but present with adult-onset diabetes.

A complementary study confirms our findings that GATA6 is necessary for endoderm specification as well as the decreased efficiency of β-like cell generation from the GATA6indel/+ PSC line (Shi et al., 2017). This other report did have one major discrepancy with our results; they did not see a defect in glucose-stimulated insulin secretion in GATA6indel/+ β-like cells. This could be due to genetic background as they examined gene-edited ES cells, not pancreas agenesis patient derived IPSCs, or due to differences in the differentiation protocols. Future studies will be needed to further address the role of GATA6 in β cell function in the PSC system.

The use of PSCs has provided a powerful human-based system to study GATA6 mutations. Important insights have been gained into the role of GATA6 in early human definitive endoderm development, pancreatic progenitor specification, and pancreatic β cell functionality.

Experimental Procedures

PSC Lines

The Mel1-INS-GFP ES cells were obtained from Ed Stanley and Andrew Elafanty at the Murdoch Children's Research Institute (Micallef et al., 2012). The CHOP.Panagenesis1 (patient IPS+/indel) IPS cells were generated from a lymphoblastoid cell line (Stanescu et al., 2014) by reprogramming using episomal vectors (Okita et al., 2011) by the Stem Cell core at the Children's Hospital of Philadelphia. PSC maintenance was performed as described previously (Paluru et al., 2013).

Genome Editing Using CRISPR/Cas

To generate GATA6 mutations in both alleles and correct the patient IPS+/indel cells, a gRNA was generated with the sequence 5′-AGT GGG CCA GCC AAC CAC GCG GG-3′ targeting the second exon of GATA6. For gene correction, a 200 bp oligonucleotide containing silent mutations in the gRNA sequence and a PstI restriction site in close proximity were transfected along with CAS9-GFP and gRNA. GFP+ cells were sorted and plated at clonal density. For INDEL mutations, single colonies were screened by PCR. For gene correction, single colonies were screened by PCR followed by a restriction digest with PstI and sequenced to confirm the correction. A more detailed protocol can be found in Supplemental Experimental Procedures.

Targeting the AAVS1 Locus

The AAVS1 loci of Mel1-INS-GFP ES cells were targeted with a vector containing a chicken actin promoter driving the reverse rtTA using a published protocol (Tiyaboonchai et al., 2014).

Pancreatic β-like Cell Differentiation and Reaggregation

Differentiation was started 5 days after EP cells were split. Differentiation to pancreatic β-like cells was performed as previously described (Cheng et al., 2012, D’Amour et al., 2006) with modifications at the end stage of the protocol (details can be found in the Supplemental Experimental Procedures). At day 13, cells were reaggregated following treatment with 1 mg/mL dispase for 15 min and replated into a low-adherent plate (Corning). The medium was changed every other day.

Glucose-Stimulated Insulin Secretion Assay

β-like cell aggregates were washed twice with Krebs-Ringer bicarbonate HEPES (KRBH) (details in Supplemental Experimental Procedures) and incubated at 37°C in 1 mL of KRBH for 1 hr. Cells were incubated in 500 μL of 1 mM glucose in KRBH for 20 min and stimulated in 500 μL of 20 mM glucose in KRBH for 20 min. Following each incubation, cells were centrifuged at 150 × g for 90 s. Supernatant was collected and stored at −20°C. C-peptide ELISA was performed using the ultra-sensitive C-peptide kit following the manufacturer's instructions (Mercordia). For the ELISA, supernatant was diluted 8- to 20-fold.

Statistical Analysis

Results from multiple experiments are expressed as the mean ± SEM. An unpaired two-tailed Student's t test for groups with equal variance was performed to determine p values. For experiments that tested different conditions on the same cell lines, a one-way ANOVA followed by Dunnett's multiple comparisons test was performed. All statistical analysis was performed on Prism version 6.0e for Mac (GraphPad Software). In the figures, *p < 0.05, **p < 0.01, and ***p < 0.001, and n denotes individual experiments.

Acknowledgments

We would like to thank Ge Liang, Helen Mac, and Jason Mills at the Stem Cell core at the Children's Hospital of Philadelphia for the generation of the CHOP.Panagenesis1 patient IPS+/indel line and cell sorting. We also thank Xin Cheng for initiating the generation of the IPS+/indel EP cell line and Stella Chou for the GATA1 cDNA construct. This work was supported by NIH grant R01 DK092113.

Notes

Published: February 9, 2017

Footnotes

Supplemental Information includes Supplemental Experimental Procedures, seven figures, and five tables and can be found with this article online at http://dx.doi.org/10.1016/j.stemcr.2016.12.026.

Supplemental Information

Document S1. Supplemental Experimental Procedures, Figures S1–S7, and Tables S1–S5:
Document S2. Article plus Supplemental Information:

References

  • Arceci R.J., King A.A., Simon M.C., Orkin S.H., Wilson D.B. Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart. Mol. Cell. Biol. 1993;13:2235–2246. [PubMed]
  • Bonnefond A., Sand O., Guerin B., Durand E., De Graeve F., Huyvaert M., Rachdi L., Kerr-Conte J., Pattou F., Vaxillaire M. GATA6 inactivating mutations are associated with heart defects and, inconsistently, with pancreatic agenesis and diabetes. Diabetologia. 2012;55:2845–2847. [PubMed]
  • Brewer A., Gove C., Davies A., McNulty C., Barrow D., Koutsourakis M., Farzaneh F., Pizzey J., Bomford A., Patient R. The human and mouse GATA-6 genes utilize two promoters and two initiation codons. J. Biol. Chem. 1999;274:38004–38016. [PubMed]
  • Carrasco M., Delgado I., Soria B., Martín F., Rojas A. GATA4 and GATA6 control mouse pancreas organogenesis. J. Clin. Invest. 2012;122:3504–3515. [PubMed]
  • Chao C.S., McKnight K.D., Cox K.L., Chang A.L., Kim S.K., Feldman B.J. Novel GATA6 mutations in patients with pancreatic agenesis and congenital heart malformations. PLoS One. 2015;10:e0118449. [PubMed]
  • Cheng X., Ying L., Lu L., Galvão A.M., Mills J.A., Lin H.C., Kotton D.N., Shen S.S., Nostro M.C., Choi J.K. Self-renewing endodermal progenitor lines generated from human pluripotent stem cells. Cell Stem Cell. 2012;10:371–384. [PubMed]
  • Czysz K., Minger S., Thomas N. DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation. PLoS One. 2015;10:e0117689. [PubMed]
  • D’Amour K.A., Agulnick A.D., Eliazer S., Kelly O.G., Kroon E., Baetge E.E. Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat. Biotechnol. 2005;23:1534–1541. [PubMed]
  • D’Amour K.A., Bang A.G., Eliazer S., Kelly O.G., Agulnick A.D., Smart N.G., Moorman M.A., Kroon E., Carpenter M.K., Baetge E.E. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat. Biotechnol. 2006;24:1392–1401. [PubMed]
  • De Franco E., Shaw-Smith C., Flanagan S.E., Shepherd M.H., Hattersley A.T., Ellard S. GATA6 mutations cause a broad phenotypic spectrum of diabetes from pancreatic agenesis to adult-onset diabetes without exocrine insufficiency. Diabetes. 2013;62:993–997. [PubMed]
  • Hockemeyer D., Soldner F., Beard C., Gao Q., Mitalipova M., DeKelver R.C., Katibah G.E., Amora R., Boydston E.A., Zeitler B. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat. Biotechnol. 2009;27:851–857. [PubMed]
  • Holtzinger A., Evans T. Gata4 regulates the formation of multiple organs. Development. 2005;132:4005–4014. [PubMed]
  • Koutsourakis M., Langeveld A., Patient R., Beddington R., Grosveld F. The transcription factor GATA6 is essential for early extraembryonic development. Development. 1999;126:723–732. [PubMed]
  • Kroon E., Martinson L.A., Kadoya K., Bang A.G., Kelly O.G., Eliazer S., Young H., Richardson M., Smart N.G., Cunningham J. Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat. Biotechnol. 2008;26:443–452. [PubMed]
  • Kubo A., Shinozaki K., Shannon J.M., Kouskoff V., Kennedy M., Woo S., Fehling H.J., Keller G. Development of definitive endoderm from embryonic stem cells in culture. Development. 2004;131:1651–1662. [PubMed]
  • Lango Allen H., Flanagan S.E., Shaw-Smith C., De Franco E., Akerman I., Caswell R., Ferrer J., Hattersley A.T., Ellard S. GATA6 haploinsufficiency causes pancreatic agenesis in humans. Nat. Genet. 2012;44:20–22. [PMC free article] [PubMed]
  • Liu C., Morrisey E.E., Whitsett J.A. GATA-6 is required for maturation of the lung in late gestation. Am. J. Physiol. Lung Cell. Mol. Physiol. 2002;283:L468–L475. [PubMed]
  • Martinelli P., Cañamero M., del Pozo N., Madriles F., Zapata A., Real F.X. Gata6 is required for complete acinar differentiation and maintenance of the exocrine pancreas in adult mice. Gut. 2013;62:1481–1488. [PubMed]
  • Mauney J.R., Ramachandran A., Yu R.N., Daley G.Q., Adam R.M., Estrada C.R. All-trans retinoic acid directs urothelial specification of murine embryonic stem cells via GATA4/6 signaling mechanisms. PLoS One. 2010;5:e11513. [PubMed]
  • Micallef S.J., Li X., Schiesser J.V., Hirst C.E., Yu Q.C., Lim S.M., Nostro M.C., Elliott D.A., Sarangi F., Harrison L.C. INS(GFP/w) human embryonic stem cells facilitate isolation of in vitro derived insulin-producing cells. Diabetologia. 2012;55:694–706. [PubMed]
  • Molkentin J.D. The zinc finger-containing transcription factors GATA-4, -5, and -6. Ubiquitously expressed regulators of tissue-specific gene expression. J. Biol. Chem. 2000;275:38949–38952. [PubMed]
  • Morrisey E.E., Ip H.S., Tang Z., Lu M.M., Parmacek M.S. GATA-5: a transcriptional activator expressed in a novel temporally and spatially-restricted pattern during embryonic development. Dev. Biol. 1997;183:21–36. [PubMed]
  • Morrisey E.E., Tang Z., Sigrist K., Lu M.M., Jiang F., Ip H.S., Parmacek M.S. GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo. Genes Dev. 1998;12:3579–3590. [PubMed]
  • Nostro M.C., Sarangi F., Ogawa S., Holtzinger A., Corneo B., Li X., Micallef S.J., Park I.-H., Basford C., Wheeler M.B. Stage-specific signaling through TGF family members and WNT regulates patterning and pancreatic specification of human pluripotent stem cells. Development (Cambridge, UK) 2011;138:1445. [PubMed]
  • Okita K., Matsumura Y., Sato Y., Okada A., Morizane A., Okamoto S., Hong H., Nakagawa M., Tanabe K., Tezuka K. A more efficient method to generate integration-free human iPS cells. Nat. Methods. 2011;8:409–412. [PubMed]
  • Pagliuca F.W., Millman J.R., Gürtler M., Segel M., Van Dervort A., Ryu J.H., Peterson Q.P., Greiner D., Melton D.A. Generation of functional human pancreatic β cells in Vitro. Cell. 2014;159:428–439. [PubMed]
  • Paluru P., Hudock K.M., Cheng X., Mills J.A., Ying L., Galvão A.M., Lu L., Tiyaboonchai A., Sim X., Sullivan S.K. The negative impact of wnt signaling on megakaryocyte and primitive erythroid progenitors derived from human embryonic stem cells. Stem Cell Res. 2013;12:441–451. [PubMed]
  • Pan F.C., Wright C. Pancreas organogenesis: from bud to plexus to gland. Dev. Dyn. 2011;240:530–565. [PubMed]
  • Ran F., Hsu P., Wright J., Agarwala V. Genome engineering using the CRISPR-Cas9 system. Nat. Protoc. 2013;8:2281–2308. [PubMed]
  • Rezania A., Bruin J.E., Riedel M.J., Mojibian M., Asadi A., Xu J., Gauvin R., Narayan K., Karanu F., O’Neil J.J. Maturation of human embryonic stem cell-derived pancreatic progenitors into functional islets capable of treating pre-existing diabetes in mice. Diabetes. 2012;61:2016–2029. [PubMed]
  • Rezania A., Bruin J.E., Xu J., Narayan K., Fox J.K., O’Neil J.J., Kieffer T.J. Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo. Stem Cells. 2013;31:2432–2442. [PubMed]
  • Rezania A., Bruin J.E., Arora P., Rubin A., Batushansky I., Asadi A., O’Dwyer S., Quiskamp N., Mojibian M., Albrecht T. Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells. Nat. Biotechnol. 2014;32:1121–1134. [PubMed]
  • Russ H.A., Parent A.V., Ringler J.J., Hennings T.G., Nair G.G., Shveygert M., Guo T., Puri S., Haataja L., Cirulli V. Controlled induction of human pancreatic progenitors produces functional beta-like cells in vitro. EMBO J. 2015;34:1759–1772. [PubMed]
  • Sartori D.J., Wilbur C.J., Long S.Y., Rankin M.M., Li C., Bradfield J.P., Hakonarson H., Grant S.F.A., Pu W.T., Kushner J.A. GATA factors promote ER integrity and β-cell survival and contribute to type 1 diabetes risk. Mol. Endocrinol. 2014;28:28–39. [PubMed]
  • Shaw-Smith C., De Franco E., Allen H.L., Batlle M., Flanagan S.E., Borowiec M., Taplin C.E., Van Alfen-Van Der Velden J., Cruz-Rojo J., De Nanclares G.P. GATA4 mutations are a cause of neonatal and childhood-onset diabetes. Diabetes. 2014;63:2888–2894. [PubMed]
  • Shi Z.-D., Lee K., Yang D., Amin S., Verma N., Li Q.V., Zhu Z., Soh C.-L., Kumar R., Evans T. Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Cell Stem Cell. 2017;20 Published online February 9, 2017. [PubMed]
  • Stanescu D.E., Hughes N., Patel P., De León D.D. A novel mutation in GATA6 causes pancreatic agenesis. Pediatr. Diabetes. 2014;16:67–70. [PubMed]
  • Steiner D.F. The proinsulin C-peptide—a multirole model. Exp. Diabesity Res. 2004;5:7–14. [PubMed]
  • Stoffers D.A., Zinkin N.T., Stanojevic V., Clarke W.L., Habener J.F. Pancreatic agenesis attributable to a single nucleotide deletion in the human IPF1 gene coding sequence. Nat. Genet. 1997;15:106–110. [PubMed]
  • Tiyaboonchai A., Mac H., Shamsedeen R., Mills J.A., Kishore S., French D.L., Gadue P. Utilization of the AAVS1 safe harbor locus for hematopoietic specific transgene expression and gene knockdown in human ES cells. Stem Cell Res. 2014;12:630–637. [PubMed]
  • Viger R.S., Guittot S.M., Anttonen M., Wilson D.B., Heikinheimo M. Role of the GATA family of transcription factors in endocrine development, function, and disease. Mol. Endocrinol. 2008;22:781–798. [PubMed]
  • Walker E.M., Thompson C.A., Kohlnhofer B.M., Faber M.L., Battle M.A. Characterization of the developing small intestine in the absence of either GATA4 or GATA6. BMC Res. Notes. 2014;7:902. [PubMed]
  • Xuan S., Borok M.J., Decker K.J., Battle M.A., Duncan S.A., Hale M.A., Macdonald R.J., Sussel L. Pancreas-specific deletion of mouse Gata4 and Gata6 causes pancreatic agenesis. J. Clin. Invest. 2012;122:3516–3528. [PubMed]
  • Zhao R., Watt A.J., Li J., Morrisey E.E., Stephen A., Luebke-wheeler J., Duncan S.A. GATA6 is essential for embryonic development of the liver but dispensable for early heart formation GATA6 is essential for embryonic development of the liver but dispensable for early heart formation. Mol. Cell. Biol. 2005;25:2622–2631. [PubMed]

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