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Nat Commun. 2017; 8: 14632.
Published online 2017 March 8. doi:  10.1038/ncomms14632
PMCID: PMC5344980

PARP1 promotes gene expression at the post-transcriptional level by modulating the RNA-binding protein HuR

Abstract

Poly(ADP-ribosyl)ation (PARylation) is mainly catalysed by poly-ADP-ribose polymerase 1 (PARP1), whose role in gene transcription modulation has been well established. Here we show that, in response to LPS exposure, PARP1 interacts with the adenylateuridylate-rich element-binding protein embryonic lethal abnormal vision-like 1 (Elavl1)/human antigen R (HuR), resulting in its PARylation, primarily at site D226. PARP inhibition and the D226 mutation impair HuR's PARylation, nucleocytoplasmic shuttling and mRNA binding. Increases in mRNA level or stability of pro-inflammatory cytokines/chemokines are abolished by PARP1 ablation or inhibition, or blocked in D226A HuR-expressing cells. The present study demonstrates a mechanism to regulate gene expression at the post-transcriptional level, and suggests that blocking the interaction of PARP1 with HuR could be a strategy to treat inflammation-related diseases that involve increased mRNA stability.

Poly(ADP-ribosyl)ation (PARylation) is an essential post-translational protein modification catalysed by poly-ADP-ribose polymerases (PARPs), a family of enzymes that polymerize ADP-ribose units from NAD+ and transfer the polymer known as poly-ADP-ribose (PAR) onto a variety of proteins1. PARP1 is currently the best understood member of the PARP family, and is affirmed as accounting for at least 85% of cellular PARP activity2. PARP1 has been implicated in a wide range of biological processes, such as maintenance of genome integrity, transcriptional regulation, energy metabolism and cell death3,4. Although originally characterized as a key factor in DNA repair and cell death pathways, PARP1's role in regulation of gene expression under basal and signal-activated conditions has been demonstrated by a wealth of studies5,6. Extensive studies have documented that the transcriptional activation constitutes the primary mode of PARP1 modulating gene expression. PARylation, which introduces massive negative charges to the linker histone H1 and core histones1,3,7,8, mediates the relaxation of the chromatin superstructure and then facilitates the recruitment of transcription machinery to the promoters or enhancers of target genes. In addition, PARP1 is involved in the activation of transcription factors such as nuclear factor-kappa B (NF-κB), activator protein 1 (AP-1) and heat-shock factor protein 1 to regulate gene expression9. A large number of studies have well addressed the involvement of PARP1 activation in inflammatory disorders via PARP1-dependent upregulation of pro-inflammatory genes9. Our previous studies reported that PARP1 binds to and modifies RelA/p65 (refs 9, 10, 11) and, therefore, promotes the NF-κB-dependent expression of pro-inflammatory cytokines.

The expression of inflammatory genes is tightly regulated by both transcriptional and post-transcriptional mechanisms because modifying messenger RNA (mRNA) stability provides rapid and flexible control, and is particularly important in coordinating the initiation and resolution of inflammation12. This urged us to investigate whether PARP1 regulates the expression of inflammatory cytokines/chemokines at the post-transcriptional level. Emerging data have revealed the roles of PARP1 in RNA metabolism. An intriguing study showed that poly(A) polymerase is PARylated during heat shock, leading to the inhibition of mRNA polyadenylation of target genes in a PARP1-dependent manner13. In the present study, macrophages were exposed to lipopolysaccharide (LPS) with or without PARP1 inhibition. Our results showed LPS-induced increase in the stability of mRNAs from pro-inflammatory genes including Cxcl2 is diminished by PARP1 inhibition/depletion. PARP1 interacts with the adenylateuridylate-rich element (ARE)-binding protein embryonic lethal abnormal vision-like 1 (Elavl1)/human antigen R (HuR) resulting in its PARylation. The increased PARylation of HuR enhances nucleocytoplasmic shuttling and mRNA binding, and promotes mRNA stability. The results presented a mechanism to regulate gene expression at the post-transcriptional level by PARP1 activation.

Results

PARP1 augments Cxcl2 expression at post-transcriptional level

To determine the stability of mRNA, a classical approach14 was used as illustrated in Supplementary Fig. 1a. Briefly, parallel cultures of murine primary peritoneal macrophages (pMϕ) were exposed to 500 ng ml−1 LPS for 1 h to boost pro-inflammatory gene expression, and then the transcription inhibitor actinomycin D (Act D) was added in media with or without LPS (±PARP inhibitor PJ34) for 4 h. The levels of remaining mRNAs were determined using Mouse Inflammatory Cytokines & Receptors PCR arrays (SABiosciences). In response to LPS, the mRNA stability of the most tested inflammatory mediators was increased, especially those encoding chemokine receptors (for example, Ccrs), C-C (for example, Ccl11) and C-X-C (for example, Cxcl1 and Cxcl13) chemokines, as well as interleukins (for example, IL1β) (Supplementary Fig. 1b,d). LPS-induced increases in the remaining mRNA levels were significantly abolished by PJ34. For example, levels of Cxcl1, Ccl11, Cxcl13 and Il1β were decreased by 2.14-, 2.17-, 3.16- and 2.29-fold, respectively (Supplementary Fig. 1c,e). Interestingly, the levels of some Ccrs (for example, Ccr4, 5, 6, 7 and 8), Cxcrs (for example, Cxcr2 and 5) and cytokines/chemokines (for example, Ifn and Cxcl11) were not affected by PARP's inactivation (Supplementary Fig. 1c,e). Cxcl1 and Cxcl2 (homologues of human growth-regulated protein (Gro) α and β, respectively) are potent attractants of neutrophils, highly relevant to innate inflammatory responses15,16, thus real-time PCR was performed to examine their remaining mRNAs individually. LPS stimulation induced ~2.5- and ~4.5-fold increases in the levels of remaining Cxcl1 and Cxcl2's mRNA, respectively, which were diminished by PJ34 administration to the basal levels (Supplementary Fig. 1f). These results verified the involvement of PARP1 in mRNA stability regulation, and also suggested Cxcl2 mRNA more susceptible to be affected by PARP1 activation.

Next, we examined the kinetics of the level of remaining Cxcl2 mRNA. The half-life of Cxcl2 mRNA in LPS-withdrawn cells significantly declined after Act D addition, whereas it was sustained in LPS-stimulated cells. PJ34 administration abrogated the increase in Cxcl2 mRNA stability induced by LPS. A two-way analysis of variance analysis indicated a significance of P<0.001. (Fig. 1a,b). Other PARP1 inhibitors, 3-aminobenzamide (3-AB) and Olaparib, exhibited the same effect (Supplementary Fig. 2a). Inhibitor targeting-off effects (for example, unspecific block of TLR/inflammasome signalling) were excluded as PJ34 did not impair the LPS-induced IRAK1 phosphorylation (Supplementary Fig. 2b). Moreover, when PARP1 expression was silenced, the remaining Cxcl2 mRNA in LPS-stimulated cells was decreased to 40%, compared with that of control short interfering RNA (siRNA)-transfected cells (Fig. 1c). Small interfering RNA targeting another sequence of PARP1 showed a similar result (Supplementary Fig. 2c). Knockdown of PARP2, a functional back-up of PARP1, had no impact on the level of remaining Cxcl2 mRNA (Supplementary Fig. 2d), specifying the role of PARP1 in maintaining mRNA stability.

Figure 1
PARP1 augments Cxcl2 expression at the post-transcriptional level via HuR.

The AREs commonly existing in the 3′-untranslated regions (UTRs) are major mRNA destabilization determinants17,18. With their binding proteins, AREs have significant physiological functions in the modulation of mRNA stability. Cxcl2 mRNA contain tandem overlapping repeats of AUUUA motifs (class I AREs)19; therefore, we investigated the implication of 3′-UTR in the modulation of Cxcl2 mRNA stability by PARP1. A reporter plasmid was constructed as described previously20 (Fig. 1d). Dual-reporter assays revealed firefly luciferase activity in cells transfected with a Cxcl2-3′-UTR construct was severely impaired (to ~3.8%) compared with that in cells transfected with pGL3-control (Fig. 1e). In parallel experiments, the firefly luciferase mRNA level was significantly decreased (to ~3%) (Fig. 1f), indicating the Cxcl2-3′-UTR was indeed a destabilizing determinant21. LPS stimulation did not affect the activity or the mRNA level of firefly luciferase in cells transfected with the pGL3-control, where the luciferase gene was constitutively transcribed. Importantly, LPS stimulation significantly increased firefly luciferase activity (~3.5-fold), as well as its mRNA level (~5-fold) in cells transfected with the Cxcl2-3′-UTR construct, which, however, were markedly diminished by PJ34 (Fig. 1e,f). The combined data implied that Cxcl2's 3′-UTR mediates PARP1's regulation of mRNA stability.

Among ARE-binding proteins, HuR, a ubiquitously expressed member of the ELAVL family of proteins, is one of the few that have been demonstrated to stabilize ARE-containing mRNAs22,23,24. In our present study, the silencing of HuR abrogated LPS-induced increases in Cxcl2 mRNA levels (Fig. 1g; Supplementary Fig. 2e). To verify that HuR mediates the role of PARP1 in stabilizing Cxcl2 mRNA, siRNA-targeting HuR or controls were delivered into cells, and then a co-transfection of the GFP-PARP1 plasmid with the above-mentioned Cxcl2-3′-UTR dual-reporter system was conducted. Cells were either exposed to LPS or not, and firefly mRNA levels were determined by real-time PCR using Renilla luciferase mRNA for calibration. LPS-induced increase in firefly mRNA level was enhanced by the overexpression of PARP1, which was eliminated by HuR silencing (Fig. 1h). Tristetraprolin, one of the dominant mRNA-destabilizing factors, was not shown to be distributed in the nuclei with or without LPS stimulation in the present study, excluding the possibility of it mediating PARP1's role (Supplementary Fig. 2f). The combined data suggested that PARP1 modulates Cxcl2 mRNA stability by acting on HuR.

HuR is associated with PARP1 in response to LPS stimulation

To gain insights into the molecular mechanism how PARP1 modulates mRNA stability via HuR, we first examined the dynamics of protein PARylation and the interaction of PARP1 with HuR in cells exposed to LPS. The content of PARylated proteins notably increased at 2 h and reached a maximum level at 5 h post LPS addition (Fig. 2a). Thus, cells were collected after 5 h of LPS stimulation, and co-immunoprecipitation (Co-IP) assays were performed. Results showed a low-level interaction of HuR and PARP1 in the extract of untreated cells. The interaction of the two proteins was increased upon LPS stimulation, which was significantly inhibited by PJ34 (Fig. 2b,c). Both PARP1 and HuR are targets of caspases and may undergo cleavage under stress. Thus, the whole-cell lysate was applied along with a molecular weight standard, which indicated that the interaction between the two molecules requires their full-length forms (Fig. 2b,c). In addition, in the presence of RNase, the HuR–PARP1 complex did not collapse, suggesting that the association of PARP1 with HuR is not mediated by HuR-bound RNA (Supplementary Fig. 3a).

Figure 2
PARP1 interacts with HuR in response to LPS exposure.

Next, we asked what domain(s) of HuR interact(s) with PARP1. HuR has three RNA recognition motifs (RRMs) through which it interacts with target mRNAs and partner proteins25. Located between RRM2 and RRM3 is a hinge region that encompasses a nucleocytoplasmic shuttling sequence (HNS), spanning residues 205–237 (ref. 26; Fig. 2d, upper). GST-HuR prokaryotic expression plasmid and domain mutants were constructed (Fig. 2d, middle), and pull-down assays were performed. While GST could not pull down any PARP1, GST-HuR exhibited a significant interaction. GST-HuR-HNS and GST-HuR-RRM3 could modestly pull down PARP1, whereas GST-HuR-RRM1 and GST-HuR-RRM2 barely showed such an interaction (Fig. 2e). Furthermore, an N-terminal truncated mutation was made (Fig. 2d, lower), and the pull-down assay showed that the deletion of RRM1 and RRM2 did not exhibit an obvious impact on the interaction of HuR with PARP1, whereas the absence of the HNS did (Fig. 2f). The combined results suggested that the interaction of HuR and PARP1 depend on the HNS and RRM3 domains.

PARP1 PARylates HuR primarily at the aspartic acid 226

We probed the HuR-associated complex precipitated from cell lysates with an antibody against PAR and probed the PAR-associated proteins with an antibody against HuR. As we expected, HuR was PARylated in extracts of LPS-exposed cells (Fig. 3a). Full membranes with molecular weight standards are shown in Supplementary Fig. 3b. Along with the absence of severe DNA damage, the length of the PAR polymer is considerably shorter, ranging from a single residue to oligo units6. PARylated HuR did not exhibit apparent shift retardation, which was also noticed with other PARylated mRNA metabolism-related proteins27. In addition, we prepared cytosolic extract (CE) and nuclear extract (NE) to perform IP assays. Results showed that the interaction of HuR with PARP1 only occurred in NE fractions and displayed a similar pattern to that shown from whole-cell lysates (Fig. 3b, right). Intriguingly, PARylation patterns of HuR in CE and NE fractions exhibited notable differences. In CE fractions, LPS stimulation resulted in an increase in PARylated HuR, which was markedly decreased by PJ34 (Fig. 3b, left). Whereas the levels of PARylated HuR in NE fractions exhibited a moderate increase compared with that in CE, which might be a consequence of nucleocytoplasmic shuttling of PARylated HuR. PJ34 also significantly inhibited HuR's PARylation in NE fractions (Fig. 3b, right). The combined results suggested that LPS stimulation enhanced the interaction of HuR and PARP1, which led to an increase in PARylation of HuR.

Figure 3
PARP1 PARylates HuR primarily at aspartic acid 226 upon LPS stimulation.

To further address which domain(s) and site(s) are PARylated, we developed an in vitro PARylation assay using GST-fused proteins as described in the Methods. First, bead-coated GST and GST-HuR were incubated with or without PARP1 in the presence or absence of PJ34 or Poly(ADP-ribose) glycohydrolase (PARG), the enzyme removing ADP-ribose units from the target proteins28,29 (Fig. 3c, left). Incubation with PARP1 resulted in strong modifications of GST-HuR (compare lanes 2 and 3), but not GST (lane 1). In the presence of PJ34 (lane 4) or PARG (lane 5), the modifications of HuR were diminished. If the soluble GST and GST-HuR eluted from the beads were applied (Fig. 3c, right), the PARylation patterns of GST-HuR were similar to that occurred on bead-bound GST-HuR (lanes 7–10). However, a notable difference was observed when GST was incubated with PARP1 (compare lanes 1 and 6). While no signal was detectable below 100 kDa, a strong smear was exhibited at the top of the lane (which also could be observed in lanes 3 and 8), indicating the autoPARylated PARP1. The combined results verified the activation of PARP1 and the specificity of HuR PARylation. Next, domain mutants were studied, and GST-HuR-HNS was strongly PARylated (Fig. 3d). Then, we further questioned which potential amino acid, lysine 191 (K191) or aspartic acid 226 (D226), was modified. An alanine substitution mutation was created (Fig. 3e). PARylation assays showed the D226, but not the K191 mutation blocked the PARylation of GST-HuR-HNS (Fig. 3f). The D226A mutation also caused barely detectable PARylation of the full-length GST-HuR (Fig. 3g). The combined results suggested that D226 is the primary site of PARylation.

PARP1 promotes the LPS-induced shuttling of HuR

It has been documented that HuR's function is regulated primarily at the level of nucleocytoplasmic shuttling19; hence we examined the effect of PARylation on HuR's distribution. Immuno-fluorescence (IF) staining showed that HuR was localized in nuclear compartments in mock-treated cells, while in LPS-exposed RAW 264.7 (Fig. 4a) and pMϕ cells (Supplementary Fig. 4a), apparently distributed to the cytoplasm. To quantify the nucleocytoplasmic shuttling of HuR, immunoblotting was performed (Supplementary Fig. 4b). Both approaches showed that the cytoplasmic localization of HuR peaked at 5 h and lasted up to 8 h after LPS stimulation, coinciding with protein PARylation kinetics (Fig. 2a). LPS-induced HuR's nuclear export was blocked by PJ34 (Fig. 4b; Supplementary Fig. 4c,d). With the increase in cytoplasmic HuR, no effective reduction of nuclear HuR occurred (Supplementary Fig. 4b), implying the increased expression of HuR in response to LPS, which was verified by a time kinetics analysis of HuR expression (peaked at 5 h post LPS addition; Supplementary Fig. 4e). Thus, to exclude that an increase in the cytoplasmic HuR level is the consequence of enhanced protein synthesis, protein synthesis inhibitor cycloheximide (CHX, 10 μg ml−1) was applied after 1 h LPS exposure. IF staining of HuR in CHX-applied cells supported the role of PARP1 in promoting HuR's nuclear export (Fig. 4c; Supplementary Fig. 4f).

Figure 4
Role of PARP1 in HuR's nucleocytoplasmic shuttling in LPS-exposed cells.

To specify the implications of PARP1, PARP1 silencing was carried out. In cells transfected with control siRNA, LPS stimulation induced a marked nuclear export of HuR (Fig. 4d, left). Whereas, when cells were transfected with siRNA-targeting PARP1, LPS-induced nucleocytoplasmic shuttling of HuR occurred only in the cells where PARP1 failed to be effectively depleted (Fig. 4d, right, note yellow arrows). When PARP1 was successfully silenced, the shuttling of HuR was nearly thoroughly blocked (Fig. 4d, right, note white arrows). These results suggested PARP1's indispensable role in regulating the shuttling of HuR. An immunoblotting analysis provided the quantification of HuR's cytoplasmic redistribution due to PARP1 interference (Supplementary Fig. 4g).

Protein PARylation enhances binding of HuR to Cxcl2 mRNA

Binding of HuR's to mRNA counteracts the destabilizing effects of tristetraprolin, TFIIB-related factor 1, KH-type splicing regulatory protein and AU-binding factor 1 (ref. 19), accounting for another aspect of its roles in mRNA protection. Thus, we performed RNA-immunoprecipitation (RNA-IP) assays as described in Methods. From the whole-cell lysate of mock-treated cells, the binding of HuR to Cxcl2 mRNA was barely detectable; however, from the LPS-challenged cell lysate, abundant Cxcl2 mRNA was pulled down. The interaction of HuR with Cxcl2 mRNA was inhibited by PJ34 (Fig. 5a). Given that HuR undergoes nucleocytoplasmic shuttling upon activation, we prepared CEs for RNA-IP assays. In the CE fraction from LPS-stimulated cells, the HuR-associated Cxcl2 mRNA level was higher than that from mock-treated cells, which was decreased by PJ34 (Fig. 5b), the levels of a set of ARE-containing mRNAs, such as Cxcl1, Cxcl13 and Il-1β, showed the similar patterns in HuR's immuno-precipitates (Supplementary Fig. 5). Intriguingly, as a control, Ccr7 mRNA, whose stability was not subjected to PARP1 regulation as shown by Inflammatory Cytokines & Receptors PCR arrays (Supplementary Fig. 1c,e), could not be pulled down with HuR (Fig. 5c). In support, the remaining level of Ccr7 mRNA was increased upon LPS exposure but not affected by PJ34 (Fig. 5d). The data suggested that protein PARylation increases the association of HuR with the mRNA targets.

Figure 5
Protein PARylation promotes the binding of HuR to Cxcl2 mRNA.

PARP1 enhances the binding of HuR to ARE-containing RNA

To further confirm that the binding of HuR to the ARE motif is regulated by PARylation, GST-HuR was used to perform RNA electrophoretic mobility shift assays (EMSA). GST-HuR or GST was purified and eluted, and then subjected to PARylation or not, followed by incubation with biotin-labelled tandem ARE repeat-containing RNA oligos. GST-HuR elicited several shifted bands, which might result from the various copies of GST-HuR harboured on the tandem ARE-containing probes (Fig. 6a, lanes 1–12), whereas GST failed to do so (Fig. 6a, lane 13). The incubation with PARP1 markedly enhanced the binding of GST-HuR with the probes (Fig. 6a, compare lanes 4–6 with lanes 1–3), which was inhibited by PJ34 (Fig. 6a, lanes 7–9). The direct incubation of eluted GST-HuR with probes showed the same patterns of the shifted bands (Fig. 6a, lanes 10–12) as that of samples subjected to PARylation (Fig. 6a, lanes 1–9); in parallel, as a vehicle control, PARP1 in PARylation buffer alone did not result in any shifted bands (Fig. 6a, lane 14). The addition of titrated cold probe resulted in a dose-dependent competition (Fig. 6b), indicating the specificity of the binding of GST-HuR. Furthermore, an antibody supershift assay was performed (Fig. 6c). Both HuR and GST antibodies led to super shifts from the protein–probe complexes (asterisk-labelled) while PARP1 antibody failed to do so, similar to IgG1. Recently, PARP1 was reported to directly interact with noncoding pRNA, binding to silent ribosomal RNA genes after their replication in the mid-late S phase30. We questioned whether the absence of interaction between PARP1 and RNA in the present study is due to strong binding of PARP1 to the sonicated DNA. We performed a gel-shift assay with titrated sonicated DNA. No shifted bands appeared with the decreasing amount of sonicated DNA (Fig. 6d), verifying no direct binding of PARP1 with the probes. Because the binding of PARP1 with noncoding pRNA relies on a hairpin structure30, we deduced PARP1 may not able to bind with single-stranded RNA.

Figure 6
PARP1 increases the binding of HuR with ARE-containing RNA.

Furthermore, isometric RNA oligos containing three AREs that exist in the native UTR domains of Cxcl2 mRNA were designed, as illustrated in Fig. 7a. An RNA-EMSA showed that GST-HuR could interact with ARE1- and ARE2-, but not ARE3-containing probes (Fig. 7b Lanes 2, 6 and 10), and incubation with PARP1 enhanced the binding of GST-HuR (Fig. 7b lanes 3 and 7), which was abolished by the PJ34 (Fig. 7b, lane 4 and 8). As a control, GST was not able to interact with any of the ARE motifs even after incubation with PARP1 (Fig. 7b, lanes 1, 5 and 9). An antibody supershift assay and cold probe competition verified the specificity of HuR's binding (Fig. 7c). Furthermore, we performed an EMSA to compare the binding of wild-type (WT) and D226A HuR to Cxcl2-ARE1 RNA oligo, and the Kd values were calculated as previously described31. After incubation with PARP1, WT HuR's binding to the Cxcl2-ARE1 RNA oligo increased by more than two folds (Kd value decreased to ~40%), while the D226A mutant displayed similar affinity kinetics to those of WT HuR without incubation with PARP1 (Fig. 7d). The combined data verified the role of PARP1 in binding of HuR to ARE-containing mRNAs.

Figure 7
PARP1 increases the binding of HuR with AREs in Cxcl2 mRNA's 3′-UTR.

D226 PARylation is crucial for HuR's function

To gain further insight into the physiopathological significance of D226-mediated HuR PARylation in an intracellular context, we constructed eukaryotic expression plasmids expressing WT Flag-HuR, as well as D226A and K191A mutants. Due to the efficiency of transfection, HEK 293/hTLR4A-MD2-CD14 Cells (HEK 293 cells stably transfected with the human TLR4, MD2 and CD14 genes) were utilized. Both human and murine Cxcl2 mRNAs contain AREs in their 3′-UTRs. IP assays using antibody recognizing Flag tag revealed that WT Flag-HuR and the K191A mutant were highly PARylated in LPS-exposed cells, whereas the D226A mutant was not (Fig. 8a). An RNA-IP assay revealed that LPS stimulation increased WT and K191A Flag-HuR-bound Cxcl2 mRNA levels, but not that with the D226A mutant (Fig. 8b). IF staining and immunoblotting analysis of both RAW 264.7 and HEK 293 cells further affirmed WT and the K191A mutant, but not D226A Flag-HuR, were able to shuttle to the cytoplasm upon the LPS challenge (Fig. 8c,d; Supplementary Fig. 6a,b). Importantly, the ectopic expression of murine WT and D226A HuR in endogenous HuR-silenced HEK 293 cells showed that HuR depletion strongly blocked LPS-induced increases in CXCL2 mRNA level, which was markedly rescued by the overexpression of murine WT but not D226A HuR (Fig. 9a,b). Further investigations into the stability of the pro-inflammatory gene's mRNA showed that the half-life of CXCL2 mRNA in WT HuR-expressing cells was ~4 h, which was reduced to ~2 h in D226A HuR-expressing cells (Fig. 9c,d). Also, the stability of CXCL1, IL-8 and TNFα mRNAs was significantly lower in D226A mutant-expressing cells than in WT HuR-expressing ones (Supplementary Fig. 7). The results indicated the functional significance of D226 PARylation of HuR in response to immune stimulation.

Figure 8
The HuR D226 mutant cannot be effectively PARylated, bind with Cxcl2 mRNA, undergo nucleocytoplasmic shuttling.
Figure 9
The HuR D226 mutant is incapable to sustain the CXCL2 mRNA level.

LPS induces HuR interaction with PARP1 and is PARylated in vivo

To investigate the inducible interaction of PARP1 with, and PARylation of HuR in an in vivo scenario, mice lungs were exposed to LPS through an intranasal route with or without a PJ34 pretreatment. LPS induced a notable increase in the protein PARylation level in mice lungs from 0.5 h and peaked at ~1 h (Fig. 10a). This induction was blocked by the PJ34 pretreatment (Fig. 10b). Accordingly, the interaction of HuR with PARP1, as well as HuR's PARylation level, markedly increased after 1 h of LPS exposure, and the PARylation was also diminished by PJ34 administration (Fig. 10c). The results implied a potential physiopathological impact of PARP1 in modulating HuR's function in response to inflammatory stimulation.

Figure 10
LPS induces association of PARP1 with and PARylation of HuR in mice lungs.

Discussion

PARP1's role in the regulation of gene expression under a variety of conditions has been well established. While a large number of studies have reported that PARP1 promotes gene transcription28, our present work demonstrated that augmentation of the stability of pro-inflammatory mediator mRNAs presenting a regulatory mechanism of PARP1 in gene expression at the post-transcriptional level.

The ‘steady-state' level of transcripts in eukaryotic cells is an outcome of the competition of RNA synthesis and degradation32. The best-studied instability elements in mammalian mRNA are the AREs33. Up to 8% of the genes in the human genome contain at least one putative ARE in their 3′-UTR34. The stability of ARE-containing mRNAs is mediated by ARE-binding proteins. Among them, the Elavl family members HuR, HuB, HuC and HuD stabilize target mRNAs and/or stimulate their translation23. In our present study, series dual-reporter assays (Fig. 1) suggested that HuR is the factor PARP1 acting to modulate the stability of ARE-containing mRNAs.

Our present study demonstrated an inflammatory stimulation-induced interaction of HuR with PARP1 and the subsequent PARylation of HuR. Recently, several groups' proteome-wide studies identified PARylation targets. A large number of PARylated substrates are involved in RNA-related metabolic processes35,36 other than chromatin structure modulation, DNA repair, transcription and cell death. Intriguingly, HuR was identified as a PARylation target under H2O2 or methyl methane sulfonate stimulation, indicating the coordination of RNA metabolic processes in response to genotoxic stress27.

The functional regulation of HuR relies on diverse post-translational modifications. To date, HuR has been identified as a substrate of serine and threonine phosphorylation by PKC, Chd2, p38 and Cdk1 (reviewed in ref. 37). However, emerging data also reported that other types of post-translational modifications, including tyrosine phosphorylation, methylation and ubiquitylation, either positively or negatively regulate the functions of HuR38,39. Our present study strikingly revealed that PARP1 binds to and PARylates HuR in cells upon LPS exposure (Figs 2, ,33 and and10).10). Pull-down assays revealed that HNS and RRM3 are involved in the interaction with PARP1. In addition, in vitro PARylation assays showed HNS was strongly modified on D226 (Fig. 3). In vivo, the D226 mutation abolished the PARylation of HuR in cells challenged by LPS (Figs 8 and and99).

HuR's function is considered to be controlled in two principal ways: (1) being mobilized from the nucleus to the cytoplasm and (2) altering its association with target mRNAs19.

So far, serine phosphorylation within HNS is the well-established mechanism regulating the nucleocytoplasmic shuttling of HuR. Cdk1 phosphorylates HuR at S202 during G2, thereby helping to retain it in the nucleus, in association with 14-3-3, and hindering its post-transcriptional function and anti-apoptotic influence26. The recruitment of HuR to the cytoplasm is enhanced by S221 phosphorylation, which is a consequence of the direct interaction of PKC-alpha with nuclear HuR in response to increases in ATP or angiotensin II40,41. Our present study provided substantial evidence to show PARP1 is indispensable for the nucleocytoplasmic shuttling of HuR (Fig. 4). D226 mutation resulted in the handicapped nucleocytoplasmic shuttling of HuR in LPS-exposed macrophages (Figs 8 and and99).

The involvement of PARylation in protein nuclear export has been addressed previously. RelA/p65 PARylation decreased its interaction with chromosomal maintenance 1 (CRM1, also known as exportin 1) upon TLR4 stimulation, leading to NF-κB nuclear retention, which ultimately influenced NF-κB-dependent gene expression42. Also, PARP1-mediated PARylation of p53 blocked the interaction of p53 with CRM1, resulting in the nuclear accumulation of p53 (ref. 43). The export of HuR to the cytoplasm is regulated mainly in a CRM1-dependent manner through its association with nuclear ligands pp32 and APRIL, which contain nuclear export signals that are recognized by the exported CRM1 (ref. 44). In addition, HuR serves as an adaptor for c-fos mRNA export through another pathway that involves the interaction of HNS with transportin 2 (ref. 44). Thus, whether the downstream pathways mediating nuclear export of PARylated HuR are CRM1-dependent or -independent requires further investigation.

Many other reports have focused on the binding of cytoplasmic HuR with target mRNA25,45 to determine its function in regulating mRNA stability. Whereas we propose that PARylated HuR might bind to ARE-containing mRNA before the mRNA is transported to the cytoplasm since both PARP1 and HuR are located in the nuclei of quiescent cells, where the interactions of the two proteins and the PARylation of HuR are induced. The binding of HuR to transcripts may also affect other nuclear events, such as splicing, polyadenylation, intracellular trafficking, translation and modulation of mRNA repression by miRNAs37. Thus, the influence of PARylation on the binding of HuR to target mRNA may have other profound effects downstream in different signalling pathways, which needs to be further explored in the future.

In addition, the inhibition of PARP1 or D226A mutations resulted in decreased binding of HuR to Cxcl2 mRNA upon LPS stimulation (Figs 5, ,6,6, ,7,7, ,8,8, ,9).9). Recombinant protein RNA-EMSA assays showed that PARP1-inflicted modifications enhanced the binding of HuR to ARE-containing RNA oligos, indicating that PARylation of HuR increases its association with AREs, thereby regulating the stability of ARE-containing mRNA. RRM1 and RRM2 are considered the major domains to interact with RNA cargos. Previous studies have shown that phosphorylation at S88 in RRM1, T118 and S158 in RRM2, S100 between RRM1 and RRM2 affects HuR binding to numerous mRNAs37. Whereas a recent study also showed that the phosphorylation of S318 in RRM3 by PKCδ affects the binding of HuR to target mRNA46. It is somewhat surprising that the modification of D226, which is located in the HNS, affected the binding of HuR to target mRNA. Our combined data from GST pull-down and in vitro PARylation assays suggested a possible mechanism in which the PARylation of D226 in HNS might lead to a conformational change, facilitating the recognition of RRM(s) to the target mRNAs. In support, the Janus kinase 3 elicited the phosphorylation of Y200, an amino acid within HNS, and also influenced the binding of HuR to SIRT1 mRNA39. However, the RNA-recognizing domains may not be PARylated (Fig. 3d) because highly negatively charged PAR chains may block the interaction with RNAs that are also negatively charged. Nevertheless, several RRM-containing proteins (for example, the heterogeneous nuclear ribonucleoprotein family47,48 and the RNA processing factors NONO and RBMX49,50) have been demonstrated as PAR readers, interacting with PARylated proteins, which adds another regulatory possibility for protein PARylation in the binding of HuR with target RNA.

An intriguing study demonstrated that upon lethal stress, HuR undergoes caspase-mediated cleavage at D226 in the cytoplasm. This cleavage activity is associated with the apoptosome activator pp32/PHAP-I, and this caspase-mediated cleavage constitutes a regulatory step that contributes to an amplified apoptotic response51. Here we deduced D226-mediated PARylation may impair the effect of the apoptosome and further influence HuR functions by slowing down its turnover rate in the cytoplasm.

In addition, the role of protein PARylation in post-transcriptional regulation of gene expression may involve other members of the human PARP family (for example, PARP-5a, -12, -13.1, -13.2, -14 and -15) at multiple levels. These cytoplasm-located PARPs recruit and modify the ARE-binding proteins52,53 or microRNA-binding Agos54, directing mRNA-carrying complexes to stress-granules, blocking mRNA translation28,55 or destabilizing target mRNA in an exosome-dependent manner53.

HuR affects cell fate by regulating the stability and/or translation of mRNAs that encode proteins contributing to the vast majority of cellular processes, including cell growth and differentiation, metabolism, migration, immune response, apoptosis, and senescence56. The stabilization of the mRNAs encoding important inflammatory mediators20,57 constitutes an important paradigm of HuR's functions. Many inflammatory mediator mRNAs known to be regulated at the stability level58,59,60,61, and shown subjected to PARP1 regulation in the present study, are ARE-containing, such as Il1β, Cxcl1, Cxcl2 and Ccl11. Moreover, although there is no study addressing its stability, Il11 mRNA contains typical AREs in its 3′-UTR, and PARP1 inhibition resulted in a significant decrease in its mRNA stability as shown by plate-based real-time PCR arrays. The combined results implied that PARP1 may regulate the stability of a group of ARE-containing mRNAs by acting on the ARE-binding protein HuR.

In summary, our present study demonstrated that binding to PARP1 and PARylation are crucial for HuR-mediated mRNA stability, thereby uncovering a new mechanism to regulate gene expression at the post-transcriptional level. Our data also suggest a potential strategy to treat diseases closely linked to increased mRNA stability, such as inflammation-related disorders and cancers, through the inhibition of the PARylation of HuR.

Methods

Antibodies and reagent

Monoclonal antibodies against PARP1 (1:2,000, B-10, sc-74470), HuR (1:2,000, 3A2, sc-5261), Histone H1 (1:1,000, AE-4, sc-8030), PARP2 (1:1,000, F-3, sc-393310), IRAK1 (1:2,000, B-5, sc-55530), rabbit p-IRAK1 (1:1,000, Ser 376, sc-325147) and goat polyclonal antibody TTP (1:1,000, N-18, sc-8458) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-β-tubulin (1:8,000, HC101) and anti-β-actin (1:8,000, HC201) mouse monoclonal antibodies were purchased from TRANS (Beijing, China). Monoclonal antibody against PAR (1:2,000, ALX-804-220) and anti-PARP1 rabbit polyclonal antibody (1:5,000, ALX-210-302-R100) were from Alexis (San Diego, CA, USA). The anti-PAR rabbit polyclonal antibody (1:2,000, 4336-BPC-100) was from Trevigen (Gaithersburg, MD, USA). The monoclonal antibody against FLAG (1:8,000, F1804) was from Sigma (Saint Louis, MO, USA). Protein synthesis inhibitor CHX (C1988), transcription inhibitor Act D (A1410), PARP1 inhibitor PJ34 (P4365), 3-AB (A0788), Ribonuclease A (R5503), PARG (SRP8023, 40 ng per 50 μl) and LPS (L2630) were from Sigma. Olaparib (AZD2281) from Selleckchem (Houston, TX, USA).

Preparation of murine peritoneal macrophages

pMϕ cells were isolated from C57BL/6J mice as described previously62,63. Briefly, 20–22 g mice were injected with 2 ml of 4% thioglycollate. Two days after the injection, peritoneal exudate cells were isolated by washing the peritoneal cavity with ice cold PBS. Cells were incubated for 2 h, and non-adherent cells were removed. The macrophages were cultured with DMEM (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum in Petri dishes for 3 days at 37 °C. More than 95% of the adherent cell population was that of macrophages, as determined by staining with monoclonal antibody F4/80 (ref. 64).

Cell culture and treatment

Murine RAW 264.7 macrophages and human embryonic kidney 293/hTLR4A-MD2-CD14 (HEK 293) cells stably transfected with the human TLR4, MD2 and CD14 genes (InvivoGen, San Diego, CA, USA). In the present study, for simplification, HEK 293 refers to this cell line. Cells were cultured in DMEM (Invitrogen) supplemented with 10% (v/v) fetal bovine serum and antibiotics. Mycoplasma contamination in the cell culture was negative detected by using CycleavePCR Mycoplasma Detection kit (Takara Bio Inc., Japan). For the immune challenge, the dose of LPS was 500 ng ml−1. To inhibit de novo transcription, cells were treated with 10 μg ml−1of Act D. The dose of the PARP 1 inhibitor PJ34 was 2.5 μM as previously described10,65. To inhibit PARP1 activation, 3-AB and Olaparib were applied at 20 and 5 μΜ to the cell culture. The dose of Rnase A was 10 μg ml−l. To inhibit protein synthesis, 10 μg ml−1 of CHX was utilized45, and 40 ng of PARG was added to the PARylation assay.

Reverse transcription and real-time PCR

Total RNA was extracted using TRIzol reagent (Invitrogen), and 1 μg of purified RNA from each sample was transcribed to complementary DNA. Primers for real-time PCR included: mCxcl2: forward: 5′-TCAATGCCTGAAGACCC-3′, reverse: 5′-TGGTTCTTCCGTTGAGG-3′; mCcr7: forward: 5′- GCGAGGACACGCTGAGAT-3′, reverse: 5′-GCCGATGAAGGCATACAA-3′; mGAPDH: forward: 5′-CTCATGACCACAGTCCATGC-3′, reverse: 5′-CACATTGGGGGTAGGAACAC-3′; and mβ-actin: forward: 5′-AACAGTCCGCCTAGAAGCAC-3′, reverse: 5′-CGATGACATCCGTAAAGACC-3′.

Stability of mRNA

To measure the effect of PARP1 activity in regulating the stability of the inflammatory mediator mRNA, a classical approach is applied14. RAW 264.7 or pMϕ cells were exposed to LPS for 1 h, and then transcription inhibitor Act D was added to the medium with or without the maintenance of LPS (±PJ34) for 0, 1, 2, 3 and 4 h. The level of mRNA was measured. Individual PCR amplification reactions were performed and analysed as described above. Plate-based inflammation-related cytokines and chemokines PCR arrays were used as suggested by the manufacturer (SABiosciences, Valencia, CA, USA).

Constructs

To construct reporter plasmids to detect the effects of the 3′-UTR on the stability of target gene mRNA, the Cxcl2-3′-UTR was amplified by PCR using murine complementary DNA as the template and was cloned into the vector pGL3-control (Promega, Madison, WI, USA). Plasmids Flag-HuR, GST and GST-HuR were kindly provided by Dr Myriam Gorospe (Laboratory of Cellular and Molecular Biology; National Institute on Aging, National Institutes of Health, USA). The domain mutations GST-HuR-RRM1, GST-HuR-RRM2, GST-HuR-HNS, GST-HuR-[open triangle]RRM1, GST-HuR-[open triangle]RRM1+RRM2 and GST-HuR-RRM3 were developed from GST-HuR. A Fast Mutagenesis System kit (FM111, TRANS) was used to produce K191A and D226A site mutations in GST-HuR-HNS, GST-HuR and Flag-HuR.

Luciferase assay

RAW 264.7 cells were seeded in the 24-well plates overnight in the absence of antibiotics. The cells were then transfected with the Cxcl2-3′-UTR luciferase reporter plasmid, control vector (pGL3-Control) and Renilla luciferase reporter plasmid (an internal control, Promega) using Lipofectamine 2000 transfection reagent (Invitrogen). Cells were challenged with or without LPS 5 h later, and then lysed in 100 μl passive lysis buffer, and the extracts (20 μl) were analysed for luciferase activity using a Dual Luciferase Reporter Assay kit (Promega). To further analyse the effects of the 3′-UTR on mRNA levels of target genes, RNA was extracted and 1 U of DNase was used per 1 μg of RNA to eliminate plasmid DNA contamination. Firefly luciferase mRNA levels were measured by real-time PCR and calibrated to that of Renilla. Primers used were as follows: firefly luciferase: forward: 5′-GGTGGACATCACTTACGC-3′, reverse: 5′-CTCACGCAGGCAGTTCTA-3′; and Renilla luciferase: forward: 5′-AGCCAGTAGCGCGGTGTATT-3′, reverse: 5′-TCAAGTAACCTATAAGAACCATTACCAGATT-3′.

siRNA

siRNAs targeting murine PARP1 (#1: 5′-CCAUCAAGAAUGAAGGAAAUU-3′, #2: 5′-UUUCCUUCAUUCUUGAUGGTTUU-3′), murine HuR (#1: 5′-CAGAAACAUUUGAGCAUUGUA-3′, #2: 5′-ACUCGCCUGCUAGGCGGUUUGGA-3′) and human HuR (5′-UGCCGUCACCAAUGUGAAAGU-3′), and siPARP2 (sc-152028, Santa Cruz Biotechnology) were used at 100 pM. Cells were seeded in plates, incubated in growth medium without antibiotics overnight, and then transiently transfected with RNA oligos using lipofectamine 2000 following the manufacturer's instructions. At 4–6 h post transfection, cells were replaced with complete medium to promote recovery.

Cell fractionation

For immunoblotting, cells were lysed in lysis buffer66 for 30 min on ice. Lysates were centrifuged at 12,000g for 20 min at 4 °C, and the supernatants were taken as the whole-cell extract (WE). Cytoplasm and nuclear fractions were prepared using the CelLytic NuCLEAR Extraction kit (Sigma) following the manufacturer's guidance. Briefly, cells were lysed with cytosolic lysis buffer for 20 min, lysates were centrifuged (11,000g, 1 min, 4 °C), and supernatants were collected (CE). The pellets were washed twice with cytosolic lysis buffer and lysed with extraction buffer. Nuclear lysates were clarified by centrifugation (21,000g, 5 min, 4 °C), and the supernatants were collected (NE).

Immunoblotting and immunoprecipitation

RAW 264.7 cells were cultured, stimulated and lysed. WE, CE and NE were prepared as described above. Then, 20 μg of protein from each sample was resolved by SDS–polyacrylamide gel electrophoresis. To carry out immunoprecipitation, WE, CE or NE extracts were cleared with protein G beads for 1 h at 4 °C before being incubated with 4 μg of monoclonal anti-HuR, PAR, PARP1 and FLAG antibodies, or, alternatively, the same amount of IgG, overnight at 4 °C. After washing, the precipitated proteins were analysed by immunoblotting66. The un-cropped scans of some important blots are supplied as Supplementary Fig. 8 in the Supplementary Information.

GST-fused protein purification and GST pull-down assay

GST and GST-fused proteins were expressed in Escherichia coli strain BL21. The induction was performed by adding 1 mM isopropyl-β-D-thiogalactopyranoside to an OD 1.0 culture at 37 °C for ~2–3 h. Whole bacteria lysates were applied to glutathione Sepharose 4B (GE Healthcare Life Science, Uppsala, Sweden), and GST-tagged proteins were purified according to the manufacturer's instructions. For pull-down experiments, GST and GST-fused proteins immobilized on 40 μl of Glutathione Sepharose 4B were incubated with 1 ml of cell extract at 4 °C for ~1–3 h. After three washes with Nonidet P-40 lysis buffer, the bound proteins were analysed by immunoblotting.

In vitro PARylation assay

A GST-fused protein in vitro PARylation assay was set up by modifying the method provided by the HT Universal Chemiluminescent PARP Assay kit (Trevigen, Gaithersburg, MD, USA). Briefly, GST and GST-fused proteins immobilized on 25 μl of glutathione Sepharose 4B were incubated with recombinant PARP enzyme and PARP cocktail at room temperature for 1 h. After three washes with Nonidet P-40 lysis buffer, the bound proteins were analysed by immunoblotting.

Immunofluorescence microscopy

Cells were fixed with 10% (v/v) formaldehyde, permeabilized with 0.5% (v/v) Triton X-100, blocked with 2% (w/v) bovine serum albumin and incubated with primary antibodies recognizing HuR (1:200), PARP1 (1:200), FLAG (1:500), TTP (1:100) or PAR (1:200). Secondary antibodies were used to detect primary antibody–antigen complexes with different colour combinations as needed. The nuclei of the cells were stained with 4,6-diamidino-2-phenylindole for 5 min. Images were acquired using a confocal microscope (Nikon, Tokyo, Japan).

To quantify the nuclear-cytoplasmic redistribution of HuR, densitometry analysis was conducted by using Image J software (version 1.44). The total HuR amount was measured first, and then that of nuclear HuR, thereby we had the cytoplasmic amount of HuR by taking nuclear amount of HuR away from that of the total. The redistribution of HuR was estimated by dividing the cytoplasmic amount of HuR by that of total.

RNA-IP

RAW 264.7 cells were exposed to 500 ng ml−1 LPS for 1 h to boost pro-inflammatory gene expression, followed by the addition of Act D. In addition, the cells were withdrawn from LPS, maintained in LPS during incubation or treated with LPS plus PJ34 for another 2 h. Then, WE and CE were pre-cleared and immune-precipitated using protein G agarose/salmon coated with an anti-HuR antibody or alternatively, the same amount of IgG (4 μg). One-half of the bead–antibody–protein/mRNA-bound complexes for each sample were washed three times and analysed by immunoblotting, and the other half was washed and used for mRNA isolation. The mRNA was isolated using an RNA sample total RNA kit (TIANGEN, Beijing, China). The level of mRNA was measured by quantitative PCR as described above. The precipitated RNA target was analysed by dividing the amount of RNA in the IP by that in the input. To determine the stability of the mRNA in the HEK 293 cells, human Cxcl2 primers were applied: forward: 5′-CAAACCGAAGTCATAGCC-3′, reverse: 5′-GAACAGCCACCAATAAGC-3′.

RNA-EMSA

GST or GST-HuR proteins were induced from an 8 ml E. coli culture with an OD 1.0 and then extracted using 70 μl of glutathione Sepharose 4B beads. The recommended proteins were purified and eluted in 100 μl buffer (50 mM Tris–HCl (pH>8.0), 100 mM KCl and 40 mM glutathione). Then, 25 μl of the eluted proteins were incubated with or without PARP1as describe previously. To perform RNA-EMSA and supershift analyses, a Chemiluminescent RNA EMSA kit (20158, Thermo Fisher Scientific, Waltham, MA USA) was used. Briefly, titrated proteins (with or without PARylation) were dissolved in the EMSA interaction buffer (3 mM MgCl2, 40 mM KCl, 5% glycerol, 2 mM DTT, 2 μg tRNA) and incubated with 20 μM of 5′ biotin-labelled RNA oligos for 40 min at room temperature. For supershift assays, 0.4 μg of specific antibodies or IgG were added to the mixture after 15 min of incubation at room temperature. The reaction mix was then loaded on to a 6% acrylamide native gel. RNA oligo probes utilized in the present study included: AU-rich RNA oligo: 5′-AUUUAUUUAUUUAUUUAUUUAUUUA-3′; Cxcl2-ARE1 RNA oligo: 5′-AGUUCACUUAUUUAUUUAUCUAUGU-3′; Cxcl2-ARE2 RNA oligo: 5′-CUAUGUAUUUAUUUAUUUAUUAAUU-3′; Cxcl2-ARE3 RNA oligo: 5′-GUGGACACAUUUAUUUAUUCAUGUA-3′. To compare the affinities of WT and D226A mutant HuR with the Cxcl2-ARE1 RNA oligo, varying concentrations of GST-HuR or GST-HuR-D226A were incubated with PARP1, or not, followed by interactions with 20 μM of 5′-biotin-labelled Cxcl2-ARE1 RNA oligo. A gel-shift was performed as described above. Band intensities were quantified, and Kd values were determined as described previously (Kd value=[protein][RNA oligo]/[complex])31.

Mouse work

Six- to eight-week-old female C57BL/6 mice (20–25 g) were purchased from Jilin University (Changchun, Jilin, China). Mice were housed in a specific pathogen-free facility at NENU (Changchun, Jilin, China) and allowed unlimited access to sterilized feed and water. They were maintained at 23±1 °C and kept under a 12-h light/dark cycle. All experiments were conducted in accordance with the Chinese Council on Animal Care Guidelines.

Mice were anaesthetized with pelltobarbitalum natricum (6.5 mg kg−1), then randomized to be challenged with LPS (50 μg per mouse in 30 μl saline) or not, using the intranasal route67, with or without an intraperitoneal pretreatment of PJ34 (10 mg kg−1) 30 min before the LPS challenge68. Mice lungs were collected, and homogenates were prepared.

Statistical analysis

All experiments were performed at least three times for each determination. Data were expressed as means±s.d.'s (n=5) and analysed by one-way or two-way analyses of variance. The level of significance was accepted at *P<0.05, **P<0.01 and ***P<0.001.

Data availability

All relevant data are available from the authors on request and/or are included with the manuscript (as Supplementary Information files).

Additional information

How to cite this article: Ke, Y. et al. PARP1 promotes gene expression at the post-transcriptional level by modulating the RNA-binding protein HuR. Nat. Commun. 8, 14632 doi: 10.1038/ncomms14632 (2017).

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Material

Supplementary Information:

Supplementary Figures

Peer Review File:

Acknowledgments

This work was supported by grants from the National Nature Science Foundation of China (grant nos 31371293 and 31571339 to X.B.), the Programme for Introducing Talent to Universities (grant no. B07017 to X.B. and X.Z.), NIH/NIEHS RO1 ES018948 (I.B.) and NIH/NIAID/AI062885 (I.B.). We thank Mardelle Susman for editing this manuscript (Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston TX, USA).

The authors declare no competing financial interests.

Author contributions X.B., I.B. and X.Z. conceived and designed the experiments, Y.K., Y.H., X.G., J.W., K.W., X.J. and X.T. performed the experiments, Y.K., X.B. and X.Z. analysed the data, Y.K. and X.B. wrote the manuscript.

03/31/2017

The original version of this Article contained an error in the spelling of post-transcriptional in the title of the paper. This has now been corrected in both the PDF and HTML versions of the Article.

References

  • Schreiber V., Dantzer F., Ame J. C. & de Murcia G. Poly(ADP-ribose): novel functions for an old molecule. Nat. Rev. Mol. Cell Biol. 7, 517–528 (2006). [PubMed]
  • Shieh W. M. et al. . Poly(ADP-ribose) polymerase null mouse cells synthesize ADP-ribose polymers. J. Biol. Chem. 273, 30069–30072 (1998). [PubMed]
  • Kraus W. L. Transcriptional control by PARP-1: chromatin modulation, enhancer-binding, coregulation, and insulation. Curr. Opin. Cell Biol. 20, 294–302 (2008). [PMC free article] [PubMed]
  • D'Amours D., Desnoyers S., D'Silva I. & Poirier G. G. Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions. Biochem. J. 342, (Pt 2): 249–268 (1999). [PubMed]
  • Heeres J. T. & Hergenrother P. J. Poly(ADP-ribose) makes a date with death. Curr. Opin. Chem. Biol. 11, 644–653 (2007). [PubMed]
  • Kraus W. L. & Lis J. T. PARP goes transcription. Cell 113, 677–683 (2003). [PubMed]
  • Poirier G. G., de Murcia G., Jongstra-Bilen J., Niedergang C. & Mandel P. Poly(ADP-ribosyl)ation of polynucleosomes causes relaxation of chromatin structure. Proc. Natl Acad. Sci. USA 79, 3423–3427 (1982). [PubMed]
  • Huletsky A. et al. . The effect of poly(ADP-ribosyl)ation on native and H1-depleted chromatin. A role of poly(ADP-ribosyl)ation on core nucleosome structure. J. Biol. Chem. 264, 8878–8886 (1989). [PubMed]
  • Ba X. & Garg N. J. Signaling mechanism of poly(ADP-ribose) polymerase-1 (PARP-1) in inflammatory diseases. Am. J. Pathol. 178, 946–955 (2011). [PubMed]
  • Liu L. et al. . Lipopolysaccharide activates ERK-PARP-1-RelA pathway and promotes nuclear factor-kappaB transcription in murine macrophages. Hum. Immunol. 73, 439–447 (2012). [PubMed]
  • Ba X., Gupta S., Davidson M. & Garg N. J. Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes. J. Biol. Chem. 285, 11596–11606 (2010). [PMC free article] [PubMed]
  • Anderson P. Post-transcriptional regulons coordinate the initiation and resolution of inflammation. Nat. Rev. Immunol. 10, 24–35 (2010). [PubMed]
  • Di Giammartino D. C., Shi Y. & Manley J. L. PARP1 represses PAP and inhibits polyadenylation during heat shock. Mol. Cell 49, 7–17 (2013). [PMC free article] [PubMed]
  • Hao S. & Baltimore D. The stability of mRNA influences the temporal order of the induction of genes encoding inflammatory molecules. Nat. Immunol. 10, 281–288 (2009). [PMC free article] [PubMed]
  • Zlotnik A. & Yoshie O. The chemokine superfamily revisited. Immunity 36, 705–716 (2012). [PMC free article] [PubMed]
  • De Filippo K. et al. . Mast cell and macrophage chemokines CXCL1/CXCL2 control the early stage of neutrophil recruitment during tissue inflammation. Blood 121, 4930–4937 (2013). [PubMed]
  • Xu N., Chen C. Y. & Shyu A. B. Modulation of the fate of cytoplasmic mRNA by AU-rich elements: key sequence features controlling mRNA deadenylation and decay. Mol. Cell. Biol. 17, 4611–4621 (1997). [PMC free article] [PubMed]
  • Zubiaga A. M., Belasco J. G. & Greenberg M. E. The nonamer UUAUUUAUU is the key AU-rich sequence motif that mediates mRNA degradation. Mol. Cell. Biol. 15, 2219–2230 (1995). [PMC free article] [PubMed]
  • Schoenberg D. R. & Maquat L. E. Regulation of cytoplasmic mRNA decay. Nat. Rev. Genet. 13, 246–259 (2012). [PMC free article] [PubMed]
  • Wang S., Zhang J., Zhang Y., Kern S. & Danner R. L. Nitric oxide-p38 MAPK signaling stabilizes mRNA through AU-rich element-dependent and -independent mechanisms. J. Leukoc. Biol. 83, 982–990 (2008). [PubMed]
  • Numahata K. et al. . Analysis of the mechanism regulating the stability of rat macrophage inflammatory protein-2 mRNA in RBL-2H3 cells. J. Cell. Biochem. 90, 976–986 (2003). [PubMed]
  • Peng S. S., Chen C. Y., Xu N. & Shyu A. B. RNA stabilization by the AU-rich element binding protein, HuR, an ELAV protein. EMBO J. 17, 3461–3470 (1998). [PubMed]
  • Good P. J., Chen Q., Warner S. J. & Herring D. C. A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator. J. Biol. Chem. 275, 28583–28592 (2000). [PubMed]
  • Fan X. C. & Steitz J. A. Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs. EMBO J. 17, 3448–3460 (1998). [PubMed]
  • Hinman M. N. & Lou H. Diverse molecular functions of Hu proteins. Cell. Mol. Life Sci. 65, 3168–3181 (2008). [PMC free article] [PubMed]
  • Kim H. H. et al. . Nuclear HuR accumulation through phosphorylation by Cdk1. Genes Dev. 22, 1804–1815 (2008). [PubMed]
  • Jungmichel S. et al. . Proteome-wide identification of poly(ADP-Ribosyl)ation targets in different genotoxic stress responses. Mol. Cell 52, 272–285 (2013). [PubMed]
  • Luo X. & Kraus W. L. On PAR with PARP: cellular stress signaling through poly(ADP-ribose) and PARP-1. Genes Dev. 26, 417–432 (2012). [PubMed]
  • Kim M. Y., Zhang T. & Kraus W. L. Poly(ADP-ribosyl)ation by PARP-1: 'PAR-laying' NAD+ into a nuclear signal. Genes Dev. 19, 1951–1967 (2005). [PubMed]
  • Guetg C., Scheifele F., Rosenthal F., Hottiger M. O. & Santoro R. Inheritance of silent rDNA chromatin is mediated by PARP1 via noncoding RNA. Mol. Cell 45, 790–800 (2012). [PubMed]
  • von Roretz C. et al. . Apoptotic-induced cleavage shifts HuR from being a promoter of survival to an activator of caspase-mediated apoptosis. Cell Death Differ. 20, 154–168 (2013). [PMC free article] [PubMed]
  • Ghosh S. & Jacobson A. RNA decay modulates gene expression and controls its fidelity. Wiley Interdiscip. Rev. RNA 1, 351–361 (2010). [PMC free article] [PubMed]
  • Chen C. Y. & Shyu A. B. AU-rich elements: characterization and importance in mRNA degradation. Trends Biochem. Sci. 20, 465–470 (1995). [PubMed]
  • Bakheet T., Williams B. R. & Khabar K. S. ARED 2.0: an update of AU-rich element mRNA database. Nucleic Acids Res. 31, 421–423 (2003). [PMC free article] [PubMed]
  • Teloni F. & Altmeyer M. Readers of poly(ADP-ribose): designed to be fit for purpose. Nucleic Acids Res. 44, 993–1006 (2016). [PMC free article] [PubMed]
  • Gagne J. P. et al. . Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. Nucleic Acids Res. 36, 6959–6976 (2008). [PMC free article] [PubMed]
  • Eberhardt W., Doller A. & Pfeilschifter J. Regulation of the mRNA-binding protein HuR by posttranslational modification: spotlight on phosphorylation. Curr. Protein Pept. Sci. 13, 380–390 (2012). [PubMed]
  • Pang L. et al. . Loss of CARM1 is linked to reduced HuR function in replicative senescence. BMC Mol. Biol. 14, 15 (2013). [PMC free article] [PubMed]
  • Yoon J. H. et al. . Tyrosine phosphorylation of HuR by JAK3 triggers dissociation and degradation of HuR target mRNAs. Nucleic Acids Res. 42, 1196–1208 (2014). [PMC free article] [PubMed]
  • Doller A. et al. . Protein kinase C alpha-dependent phosphorylation of the mRNA-stabilizing factor HuR: implications for posttranscriptional regulation of cyclooxygenase-2. Mol. Biol. Cell 18, 2137–2148 (2007). [PMC free article] [PubMed]
  • Doller A. et al. . Posttranslational modification of the AU-rich element binding protein HuR by protein kinase Cdelta elicits angiotensin II-induced stabilization and nuclear export of cyclooxygenase 2 mRNA. Mol. Cell. Biol. 28, 2608–2625 (2008). [PMC free article] [PubMed]
  • Zerfaoui M. et al. . Poly(ADP-ribose) polymerase-1 is a determining factor in Crm1-mediated nuclear export and retention of p65 NF-kappa B upon TLR4 stimulation. J. Immunol. 185, 1894–1902 (2010). [PMC free article] [PubMed]
  • Kanai M. et al. . Inhibition of Crm1-p53 interaction and nuclear export of p53 by poly(ADP-ribosyl)ation. Nat. Cell Biol. 9, 1175–1183 (2007). [PubMed]
  • Gallouzi I. E. & Steitz J. A. Delineation of mRNA export pathways by the use of cell-permeable peptides. Science 294, 1895–1901 (2001). [PubMed]
  • Kim H. H., Yang X., Kuwano Y. & Gorospe M. Modification at HuR(S242) alters HuR localization and proliferative influence. Cell Cycle 7, 3371–3377 (2008). [PMC free article] [PubMed]
  • Doller A. et al. . High-constitutive HuR phosphorylation at Ser 318 by PKC{delta} propagates tumor relevant functions in colon carcinoma cells. Carcinogenesis 32, 676–685 (2011). [PubMed]
  • Gagne J. P., Hunter J. M., Labrecque B., Chabot B. & Poirier G. G. A proteomic approach to the identification of heterogeneous nuclear ribonucleoproteins as a new family of poly(ADP-ribose)-binding proteins. Biochem. J. 371, 331–340 (2003). [PubMed]
  • Ji Y. & Tulin A. V. Poly(ADP-ribosyl)ation of heterogeneous nuclear ribonucleoproteins modulates splicing. Nucleic Acids Res. 37, 3501–3513 (2009). [PMC free article] [PubMed]
  • Krietsch J. et al. . PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks. Nucleic Acids Res. 40, 10287–10301 (2012). [PMC free article] [PubMed]
  • Adamson B., Smogorzewska A., Sigoillot F. D., King R. W. & Elledge S. J. A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Nat. Cell Biol. 14, 318–328 (2012). [PMC free article] [PubMed]
  • Mazroui R. et al. . Caspase-mediated cleavage of HuR in the cytoplasm contributes to pp32/PHAP-I regulation of apoptosis. J. Cell Biol. 180, 113–127 (2008). [PMC free article] [PubMed]
  • Iqbal M. B. et al. . PARP-14 combines with tristetraprolin in the selective posttranscriptional control of macrophage tissue factor expression. Blood 124, 3646–3655 (2014). [PubMed]
  • Todorova T., Bock F. J. & Chang P. PARP13 regulates cellular mRNA post-transcriptionally and functions as a pro-apoptotic factor by destabilizing TRAILR4 transcript. Nat. Commun. 5, 5362 (2014). [PMC free article] [PubMed]
  • Leung A. K. et al. . Poly(ADP-ribose) regulates stress responses and microRNA activity in the cytoplasm. Mol. Cell 42, 489–499 (2011). [PMC free article] [PubMed]
  • Welsby I. et al. . PARP12, an interferon-stimulated gene involved in the control of protein translation and inflammation. J. Biol. Chem. 289, 26642–26657 (2014). [PMC free article] [PubMed]
  • Doller A., Pfeilschifter J. & Eberhardt W. Signalling pathways regulating nucleo-cytoplasmic shuttling of the mRNA-binding protein HuR. Cell. Signal. 20, 2165–2173 (2008). [PubMed]
  • Tiedje C. et al. . The p38/MK2-driven exchange between tristetraprolin and HuR regulates AU-rich element-dependent translation. PLoS Genet. 8, e1002977 (2012). [PMC free article] [PubMed]
  • Atasoy U. et al. . Regulation of eotaxin gene expression by TNF-alpha and IL-4 through mRNA stabilization: involvement of the RNA-binding protein HuR. J. Immunol. 171, 4369–4378 (2003). [PubMed]
  • Herjan T. et al. . HuR is required for IL-17-induced Act1-mediated CXCL1 and CXCL5 mRNA stabilization. J. Immunol. 191, 640–649 (2013). [PMC free article] [PubMed]
  • Kastelic T. et al. . Induction of rapid IL-1 beta mRNA degradation in THP-1 cells mediated through the AU-rich region in the 3'UTR by a radicicol analogue. Cytokine 8, 751–761 (1996). [PubMed]
  • Marcais A. et al. . Maintenance of CCL5 mRNA stores by post-effector and memory CD8 T cells is dependent on transcription and is coupled to increased mRNA stability. Eur. J. Immunol. 36, 2745–2754 (2006). [PubMed]
  • Nomura F. et al. . Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression. J. Immunol. 164, 3476–3479 (2000). [PubMed]
  • Ypey D. L. & Clapham D. E. Development of a delayed outward-rectifying K+ conductance in cultured mouse peritoneal macrophages. Proc. Natl Acad. Sci. USA 81, 3083–3087 (1984). [PubMed]
  • Austyn J. M. & Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11, 805–815 (1981). [PubMed]
  • Li H. Y. et al. . Daidzein suppresses pro-inflammatory chemokine Cxcl2 transcription in TNF-alpha-stimulated murine lung epithelial cells via depressing PARP-1 activity. Acta Pharmacol. Sin. 35, 496–503 (2014). [PMC free article] [PubMed]
  • Ba X. et al. . 8-oxoguanine DNA glycosylase-1 augments proinflammatory gene expression by facilitating the recruitment of site-specific transcription factors. J. Immunol. 192, 2384–2394 (2014). [PMC free article] [PubMed]
  • Zerfaoui M. et al. . Effects of PARP-1 deficiency on airway inflammatory cell recruitment in response to LPS or TNF: differential effects on CXCR2 ligands and duffy antigen receptor for chemokines. J. Leukoc. Biol. 86, 1385–1392 (2009). [PubMed]
  • Mota R. A. et al. . Inhibition of poly(ADP-ribose) polymerase attenuates the severity of acute pancreatitis and associated lung injury. Lab. Invest. 85, 1250–1262 (2005). [PubMed]

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