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Figure 5

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NOB1 reversed the impaired EOC cell growth and metastasis mediated by miR-215. SKOV3 cells were cotransfected with miR-215 mimic or miR-NC and NOB1 overexpression plasmids (pCDNA3.1-NOB1) or blank vector (pCDNA3.1). The following experiments were performed with the above cells. A, B. Expression of NOB1 on mRNA and protein level was measured by qRT-PCR and western blotting, respectively. GAPDH was used as internal control. C-F. Cell proliferation, colony formation, migration and invasion were determined in above cells. *P<0.05, **P<0.01 compared to miR-215 + Vector.

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