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Figure 3

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NOB1 is a target of miR-215 in EOC cells. A. Conservation of the miR-215-targeting sites in the NOB1 3’UTR and the miR-215 mutant sequence that abrogates miR-215 binding to target mRNA. B. Luciferase reporter assays in SKOV3 cellsafter co-transfected with wild-type or mutant 3’UTR of NOB1 and miR-215 mimic or miR-NC. Wt: wide-type, Mut: Mutant-type. *P<0.05, **P<0.01 compared to miR-NC. C, D. NOB1 expression on mRNA level and protein level in SKOV3 cells transfected with miR-215 mimic or miR-NC. GAPDH was used as internal control. *P<0.05, **P<0.01 compared to miR-NC. E. NOB1 mRNA expression in human EOC tissues and corresponding adjacent normal tissues (ANT) was determined by qRT-PCR. GAPDH was used as an internal control. **P<0.01 compared to ANT. F. The reverse relationship between NOB1 mRNA expression and miR-215 expression was analyzed in EOC tissues by Pearson correlation analysis.

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