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PLoS One. 2017; 12(3): e0173546.
Published online 2017 March 3. doi:  10.1371/journal.pone.0173546
PMCID: PMC5336281

Correction: Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes

There are errors in the fourth and fifth sentences of the Quantification by qSeq subsection of the Materials and Methods section. The correct sentences are: The SPE conditions consisted of initial denaturation at 98°C for 2 min, cooling to 55°C at 0.3°C/s, and elongation at 68°C for 10 min. Subsequently, excess primers were digested by addition of 4 μl of Exonuclease I (5 U/μl, TaKaRa Bio) and incubation at 37°C for 120 min, followed by inactivation of the enzyme by incubation at 80°C for 30 min.

Reference

1. Hoshino T, Inagaki F (2017) Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes. PLoS ONE 12(1): e0169431 doi:10.1371/journal.pone.0169431 [PMC free article] [PubMed]

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