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From:
Published online 2017 March 3. doi: 10.3389/fimmu.2017.00204

Figure 3

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Identification of distinct subsets of primed DHSs (pDHSs) and inducible DHSs (iDHSs) in mouse T cells by DNase-Seq and ChIP-Seq. (A) Genome-wide profiling of pDHSs enriched in CD4+ T blast cells relative to naïve T cells (10). Profiles are shown for the ~17,000 strongest DNase I hypersensitive sites (DHSs) present in naïve and/or T blast cells, centered on a 2 kb window and ranked in order of increasing intensity in T blast cells. Shown alongside are the positions of predicted binding motifs for AP-1, and data from parallel JUNB ChIP-Seq analyses of AP-1 binding for the same DHSs in T blast cells before and after stimulation with PMA and calcium ionophore. (B) Genome-wide profiling of pDHSs enriched in CD4+ memory T cells relative to naïve T cells (10). Profiles are shown for all DHSs centered on a 2 kb window and ranked in order of increasing intensity in memory T cells. Shown alongside is a heat map depicting the fold change in mRNA expression of the genes nearest to these DHSs following stimulation with PMA and calcium ionophore. (C) Genome-wide profiling of iDHSs enriched in stimulated CD4+ T blast cells relative to unstimulated cells (10). Profiles are shown for all DHSs centered on a 2-kb window and ranked in order of increasing intensity in stimulated T blast cells. Shown alongside are the positions of predicted AP-1 and composite NFAT/AP-1 binding motifs, and data from parallel JUNB ChIP-Seq analyses of AP-1 binding for the same DHSs in T blast cells before and after stimulation with PMA and calcium ionophore, plus a heat map depicting the fold change in mRNA expression of the genes nearest to these DHSs following stimulation. (D) HOMER analysis of transcription factor-binding motifs enriched in either pDHSs or iDHSs (10).

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