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Genome Announc. 2017 March; 5(9): e01750-16.
Published online 2017 March 2. doi:  10.1128/genomeA.01750-16
PMCID: PMC5334599

New Virus Genome Sequences of the Guama Serogroup (Genus Orthobunyavirus, Family Bunyaviridae), Isolated in the Brazilian Amazon Region

ABSTRACT

This is the first announcement of two nearly complete viral genome sequences belonging to the Guama serogroup (genus Orthobunyavirus, family Bunyaviridae) isolated in the Brazilian Amazon region: Mirim virus (MIRV; BEAN7722) and Ananindeua virus (ANUV; BEAN109303).

GENOME ANNOUNCEMENT

The family Bunyaviridae is composed of five genera, Orthobunyavirus, Nairovirus, Phlebovirus, Hantavirus, and Tospovirus. The Guama serogroup belongs to the Orthobunyavirus genus, composed of tripartite, single-stranded, and negative-sense RNA genomes, namely, large (L), medium (M), and small (S) genomes. The L-RNA encodes the RNA-dependent RNA polymerase. The M-RNA encodes two glycoproteins, Gn and Gc, and a nonstructural protein, NSm. The S-RNA encodes the nucleocapsid protein (N) and usually a nonstructural protein, NSs (1).

The Guama serogroup was originally described in 1961 by Whitman and Casals, and the first identified viruses were Guama virus (GMAV) and Catu virus (CATUV). Nowadays, this group of viruses has other members identified: Mirim virus (MIRV), Ananindeua virus (ANUV), Timboteua virus (TBTV), Bertioga virus (BERV), Cananeia virus (CNAV), Itimirim virus (ITIV), Guaratuba virus (GTBV), and Mahogany hammock virus (MHV). Two of these viruses, GMAV and CATUV, present relevance for public health because they cause acute febrile disease in humans characterized by fever of sudden onset, headache, arthralgia, myalgia, photophobia, asthenia, and other symptoms (1,3).

The MIRV BEAN7722 was isolated in 1957 from a sentinel monkey (Cebus apella) in a forest area of the Agronômico do Norte Institute (now Brazilian Agricultural Research Corporation [Embrapa]), in Belém, Pará, Brazil. The ANUV BEAN109303 was isolated in 1966 from a marsupial (Caluromys philander) in Brazil (Utinga Forest, Belém municipality, Pará State) (2, 4).

Brain tissues of newborn mice infected with prototype strains of MIRV and ANUV were propagated into Vero cells, and after 2 to 6 days postinfection, when the cells were showing approximately 90% cytopathic effect (CPE), the supernatant was harvested and treated with DNase to digest cellular DNAs. The RNA was extracted by MagNA Pure LC 2.0 equipment (Roche Life Science) using the MagNA Pure LC total nucleic acid isolation kit (Roche Life Science). Afterward, we followed all steps of the 454 pyrosequencing protocols (Roche): cDNA library construction, emulsion PCR-titration, emulsion PCR, and sequencing (5).

The sequences were assembled using the GS de novo Assembler (Newbler version 2.6), and analyses were realized using the software Geneious version 6.1.4. Here, we announced the first report of complete genome sequence (coding region) of the orthobunyaviruses MIRV and ANUV.

Accession number(s).

This whole-genome shotgun project has been deposited in GenBank under the following accession numbers: MIRV, KY013487 to KY013489, and ANUV, KY013484 to KY013486.

ACKNOWLEDGMENTS

We thank Instituto Evandro Chagas, Ministry of Health, and Amazonia Paraense Foundation (FAPESPA) in Brazil.

Footnotes

Citation Carvalho VL, Nunes MRT, Medeiros DBA, da Silva SP, Lima CPS, Inada DT, Cardoso JF, Vianez JLSG, Rodrigues SG, Vasconcelos PFC. 2017. New virus genome sequences of the Guama serogroup (genus Orthobunyavirus, family Bunyaviridae), isolated in the Brazilian Amazon region. Genome Announc 5:e01750-16. https://doi.org/10.1128/genomeA.01750-16.

REFERENCES

1. King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ 2012. Virus taxonomy: classification and nomenclature of viruses, 9th ed. Elsevier, San Diego, CA.
2. Travassos da Rosa J, Travassos da Rosa APA, Vasconcelos P, Pinheiro F, Rodrigues S, Travassos da Rosa E, Dias L, Cruz A 1998. Arboviroses isolated in the Evandro Chagas Institute, including some described for the first time in the Brazilian Amazon region, their known hosts, and their pathology for man, p 19–31. In Travassos da Rosa APA, Vasconcelos PFC, Travassos da Rosa JFS (ed), An overview of Arbovirology in Brazil and neighbouring countries, 1st ed. Instituto Evandro Chagas, Belem, Brazil.
3. DE Souza Lopes O, DE Abreu Sacchetta L, Fonseca IE, Lacerda JP 1975. Bertioga (Guama group) and Anhembi (Bunyamwera group), two new arboviruses isolated in São Paulo, Brazil. Am J Trop Med Hyg 24:131–134. [PubMed]
4. Calisher CH, Coimbra TL, Lopez Ode S, Muth DJ, Sacchetta Lde A, Francy DB, Lazuick JS, Cropp CB 1983. Identification of new Guama and Group C serogroup bunyaviruses and an ungrouped virus from southern Brazil. Am J Trop Med Hyg 32:424–431. [PubMed]
5. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R 2005. Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:376–380. doi:.10.1038/nature03959 [PMC free article] [PubMed] [Cross Ref]

Articles from Genome Announcements are provided here courtesy of American Society for Microbiology (ASM)