Search tips
Search criteria 


Logo of emborepLink to Publisher's site
EMBO Rep. 2017 March; 18(3): 451–463.
Published online 2017 February 7. doi:  10.15252/embr.201642931
PMCID: PMC5331265
Article, Articles

L‐OPA1 regulates mitoflash biogenesis independently from membrane fusion


Mitochondrial flashes mediated by optic atrophy 1 (OPA1) fusion protein are bioenergetic responses to stochastic drops in mitochondrial membrane potential (Δψm) whose origin is unclear. Using structurally distinct genetically encoded pH‐sensitive probes, we confirm that flashes are matrix alkalinization transients, thereby establishing the pH nature of these events, which we renamed “mitopHlashes”. Probes located in cristae or intermembrane space as verified by electron microscopy do not report pH changes during Δψm drops or respiratory chain inhibition. Opa1 ablation does not alter Δψm fluctuations but drastically decreases the efficiency of mitopHlash/Δψm coupling, which is restored by re‐expressing fusion‐deficient OPA1K301A and preserved in cells lacking the outer‐membrane fusion proteins MFN1/2 or the OPA1 proteases OMA1 and YME1L, indicating that mitochondrial membrane fusion and OPA1 proteolytic processing are dispensable. pH/Δψm uncoupling occurs early during staurosporine‐induced apoptosis and is mitigated by OPA1 overexpression, suggesting that OPA1 maintains mitopHlash competence during stress conditions. We propose that OPA1 stabilizes respiratory chain supercomplexes in a conformation that enables respiring mitochondria to compensate a drop in Δψm by an explosive matrix pH flash.

Keywords: bioenergetics, membrane fusion, mitoflash, OPA1
Subject Categories: Autophagy & Cell Death, Membrane & Intracellular Transport


Proton transfer across the inner membrane of mitochondria (IMM) couples the oxidation of carbohydrates and fat to the synthesis of adenosine triphosphate (ATP) that cells use as an energy source. During oxidative phosphorylation, the flow of electrons along four respiratory complexes of the electron transport chain (CI to CIV) is coupled to the flux of protons from the mitochondrial matrix to the cristae 1, 2, 3, creating a proton‐motive force (Δp) across the IMM composed of an electrical component, the mitochondrial membrane potential (Δψm ~180 mV, negative inside), and a chemical component, the transmembrane pH gradient (ΔpHm ~0.8, alkaline inside) 4, 5. The Δp drives protons back to the matrix across the F 0 F 1 ATP synthase or complex V (CV), thereby generating ATP 1, 6. In the cristae, the complexes of the electron transport chain assemble into functional quaternary structures termed respiratory chain supercomplexes (RCS) with enhanced electron transport and respiration efficiency 7, 8, 9.

The assembly and stability of RCS depends on the mitochondrial‐shaping protein optic atrophy 1 (OPA1), an inner membrane protein which forms oligomeric complexes to sustain cristae architecture 10, 11. OPA1 is a dynamin‐related GTPase whose best characterized function is to coordinate the fusion of mitochondrial inner membranes 12, 13, 14, while the fusion of mitochondrial outer membranes is mediated by mitofusin proteins MFN1 and MFN2 15, 16, 17. The OPA1 gene is alternatively spliced at exons 4, 4a, and 5b leading to the expression of eight variants that differ in their ability to trigger membrane fusion and in their resistance to apoptosis 18, 19, 20. These variants can be constitutively cleaved by the IMM peptidase OMA1 and the i‐AAA protease YME1L at sites located in exon 5 and 5b, respectively 21, 22, 23. OPA1 cleavage leads to the production of short, soluble forms of OPA1 (S‐OPA1) that interact with uncleaved forms of OPA1 (L‐OPA1) at cristae junctions and with subunits of the mitochondrial contact site (MICOS) complex involved in the maintenance of cristae structure 24, 25, 26. Recent findings also suggest that S‐OPA1 is involved in IMM fission 27.

Respiring mitochondria exhibit spontaneous bursts of chemical and electrical activity, a phenomenon known as mitochondrial flash (mitoflash) which we recently showed to be dependent on OPA1 28. Mitoflashes detected with the circularly permuted yellow fluorescent protein targeted to mitochondria (mito‐cpYFP) were initially interpreted as bursts of superoxide production 29 but subsequent studies revealed that cpYFP reports pH changes and is insensitive to superoxide 30, 31, 32, 33. Mitoflashes are readily detected with mito‐sypHer, a cpYFP‐derived probe with enhanced pH sensitivity, and correspond to reversible alkalinizations of the matrix of ~0.4 pH units that coincide in time and in space with transient Δψm depolarization events 28, 29. These opposite changes in the electrical and chemical components of Δp compensate each other thermodynamically, and clamping Δψm at different potentials evokes compensatory matrix pH (pHmito) changes. This indicates that transient matrix alkalinizations preserve the ability of mitochondria to convert energy during drops in Δψm 28.

Mitoflashes were reported in a wide range of cells in vitro and in intact heart and skeletal muscle 29, 34 and shown to require a functional respiratory chain 28, 29, but their mechanistic basis remains unclear. The Δψm drops that trigger mitoflashes are thought to reflect transient opening of the mitochondrial permeability transition pore (mPTP), but the impact of mPTP modulators on mitoflashes varies depending on the cell or tissue tested 29, 34, 35. Earlier studies proposed that Δψm fluctuations reflect local Ca2+ or Na+ elevations 36, 37, 38, 39, opening of mPTP 36, 38, 40, 41, coupling of Δψm to the ATP synthase 42, or switching between active and inactive states of oxidative phosphorylation 43. Hyperosmotic or oxidative stress and ROS‐dependent apoptosis are consistently associated with increased mitoflash frequency, defining mitoflashes as quantitative bioindicators of cellular dysfunctions 35, 44, 45, 46. In Caenorhabditis elegans, mitoflash frequency correlates with life span and worms with low mitoflash activity live significantly longer than those with high mitoflash activity, highlighting the physiological relevance of these events 47.

In this study, we sought to clarify the mechanism of mitoflash generation by tracking the transfer of protons across the IMM. Using different fluorescent pH sensors targeted to mitochondrial compartments, we consistently observed matrix alkalinization transients, establishing the pH nature of the flashes, which we renamed “mitopHlashes”. However, we failed to detect pH changes in the cristae or in the intermembrane space (IMS) during drops in Δψm. We further show that OPA1 is not required for stochastic Δψm fluctuations but allows efficient generation of mitopHlashes, that L‐OPA1 isoforms can restore flashing independently of mitochondrial fusion, and that loss of OPA1‐mediated mitopHlash/Δψm coupling is an early marker of apoptosis.


Mitoflashes are transient matrix alkalinization events

The mitoflash probe cpYFP is pH‐sensitive and does not respond to superoxide 31, 33 but the notion that mitoflashes reflect bursts of superoxide still persists 29, 47, 48, 49. To confirm the pH nature of mitoflashes, we used two pH‐sensitive proteins structurally distinct from cpYFP: pHred and the super‐ecliptic pHluorin (spHluorin). pHred is a ratiometric pH indicator derived from the red fluorescent protein mKeima 50, and spHluorin is a non‐ratiometric pH probe derived from GFP 51. We targeted these probes to the mitochondrial matrix by fusing pHred to the signal sequence of the cytochrome c oxidase subunit VIII (Cox8) and spHluorin to the C‐terminus of the matrix soluble protein peptidase (MPP) or to a subunit of the CV facing the matrix (CV[Latin small letter gamma]) (Rieger B et al, in preparation) (Fig EV1A). The mitochondrial localization of the three pH sensors was verified by confocal microscopy (Fig (Fig1A–C).1A–C). All probes co‐localized with the resident mitochondrial protein Hsp60 with Manders and Pearson coefficient around 80% (Fig EV1B–D). pH titration curves were obtained by exposing transfected HeLa cells to nigericin and monensin to equilibrate the matrix pH with the external pH (Fig (Fig1D).1D). Increasing the pH from 5 to 8 decreased the pHred intensity ratio (F 561/F 405) by more than 10‐fold with the expected pKa of 6.7 50, while the fluorescence of MPP‐ and CV[Latin small letter gamma]‐spHluorin increased with a pKa of 7.2. The average resting pH values reported with Cox8‐pHred, MPP‐ and CV[Latin small letter gamma]‐spHluorin at 37°C were 7.75 ± 0.42, 7.90 ± 0.32 and 7.85 ± 0.34, respectively (mean ± SD), consistent with previous matrix pH measurements 52, 53, 54, 55. Addition of antimycin A, an inhibitor of the respiratory complex III, evoked fluorescence changes corresponding to an acidification (Figs (Figs1F1F and EV2A and B) in 96% of cells expressing Cox8‐pHred (n = 29) and in 70% of cells expressing MPP‐spHluorin (n = 57) or CV[Latin small letter gamma]‐spHluorin (n = 47), indicating that the three probes report dynamic changes in matrix pH. We then tested whether mitoflashes could be recorded with these matrix pH sensors. Antiparallel fluorescence transients occurring spontaneously and randomly in space were readily detected with Cox8‐pHred, reporting matrix pH flashes phenocopying the mitoflashes (Fig (Fig1G1G and Movie EV1). Reversible fluorescence increases corresponding to transient alkalinization transients were detected in single mitochondria of cells expressing spHluorin fused to CV[Latin small letter gamma] and MPP. Simultaneous recording of pH and Δψm using these probes and tetramethyl rhodamine methyl ester (TMRM) confirmed that the matrix pH flashes always occurred concomitantly with drops in Δψm without any lag observed between the initiation phase of these two signals (Fig (Fig1H1H and I, and Movie EV2). The decay phases of pH and Δψm transients did not always mirror each other as mitochondrial potential sometimes exhibited a delayed recovery. The kinetics pH flashes reported by the new probes and by the previously validated matrix pH probe mito‐sypHer were comparable, the probes reporting events of similar duration (Cox8‐pHred: 7.68 ± 1.79 s, CV[Latin small letter gamma]‐spHluorin: 8.2 ± 1.98 s, MPP‐spHluorin: 7.35 ± 1.26 s, and mito‐sypHer: 8.6 ± 0.6 s) and time to peak (Cox8‐pHred: 3.06 ± 1.12 s, CV[Latin small letter gamma]‐spHluorin: 1.91 ± 0.83 s, MPP‐spHluorin: 1.70 ± 0.44 s, and mito‐sypHer: 1.63 ± 0.6 s). The flashing area measured with all the sensors was comparable and drastically increased upon enforced mitochondrial fusion with a dominant negative mutant of dynamin‐related protein 1 (DRP1K38A, data not shown), consistent with our previous findings 28. Thus, pH flash events sharing similar spatial and temporal properties are detected in the matrix of mitochondria with structurally distinct pH sensors. This establishes pH as the main contributor of mitochondrial flashes, which we therefore renamed “mitopHlashes”.

Figure EV1
Mitochondrial localization of the different pH sensors used in this study
Figure 1
Matrix alkalinization “flashes” detected with structurally different pH sensors
Figure EV2
The IMS and cristae probes report chronic, but not acute pH changes

MitopHlashes are not detectable in the IMS and in cristae

We 28 and others 29, 31 previously postulated that mitopHlashes reflect a burst in proton pumping by the respiratory chain. To validate this hypothesis, we used pH‐sensing proteins addressed to the cristae space and the IMS to detect acidifying bursts in these compartments. Two cristae pH probes based on spHluorin fused to the C‐terminal of the subunit 8a of complex IV (CIV8) and to the subunit e of complex V (CVe) were kindly provided by Karin Busch 56, and we fused ratiometric pHluorin (rpHluorin 51) to the mitochondrial localization sequence of Smac (Smac‐rpHluorin) to measure IMS pH (Fig EV1). The probes exhibited a mitochondrial pattern when transfected cells were imaged by confocal microscopy (Fig (Fig2A–C)2A–C) and co‐localized with the endogenous marker Hsp60 (Fig EV1B–D). pH calibrations revealed that spHluorin fluorescence increased upon alkalinization with a pKa of 7.2, while the Smac‐rpHluorin F 488/F 405 ratio decreased with a pKa of 7.1 (Fig (Fig2D).2D). The resting pH values reported by these sensors were more acidic than those obtained with the probes targeted to the matrix, consistent with their predicted localization in the cristae and IMS (Fig (Fig2E).2E). However, the intensiometric cristae probes reported values more alkaline than the ratiometric IMS probe (Smac‐rpHluorin: 6.98 ± 0.34, CVe‐spHluorin: 7.49 ± 0.22 and CIV8‐spHluorin: 7.51 ± 0.21, average ± SD) or than an earlier study reporting a resting cristae pH of 7.2 56. We then checked the effects of acute inhibition of respiratory complexes. Inhibition of complex III with antimycin, expected to alkalinize the IMS and cristae, did not alter the smac‐rpHluorin ratio (Fig (Fig2F)2F) or the fluorescence of the two cristae spHluorins (Fig EV2C and D). Inhibition of complex V with oligomycin was also without effect (Fig EV2E). However, when cells were switched to a galactose‐rich or to a low glucose media, the resting pHmito values reported with CVe‐spHluorin decreased to 7.25 ± 0.06 and 6.97 ± 0.06, respectively (Fig EV2F), indicating that the probe reports chronic changes in the pH of cristae. Given the large amplitude of matrix mitopHlashes, we expected to detect variations in IMS and cristae pH in mitochondria exhibiting stochastic depolarization transients. Contrary to this prediction, time‐resolved measurements did not reveal any transient pH change during Δψm drops (Fig (Fig2G–I2G–I and Movie EV3), and mitopHlashes remained undetectable in cells grown in galactose to boost aerobic metabolism (Fig EV2G). To enhance the detection of potential mitoflash activity in the cristae or the IMS, we next expressed the DRP1 mutant DRP1K38A, which extends the connectivity of mitochondria and increases the flashing areas (Fig EV3) 28, 57, 58. Although DRP1K38A expression led to mitochondrial depolarization transients that could spread over the entire mitochondrial network, pH changes remained undetectable in the cristae or the IMS during Δψm drops (Fig EV3). To verify that the expression of proteins fused to respiratory chain complexes does not inhibit flashing activity, we co‐expressed the matrix‐targeted pH sensor mito‐sypHer together with the cristae and IMS probes. The same frequency of mitopHlashes was recorded in cells expressing cristae or IMS fusion proteins, indicating that flashing activity is maintained in these conditions (Fig EV2H). We then considered that the failure to detect acute pH changes in the IMS and cristae could reflect improper localization of the genetically encoded pH sensors, and performed immunoelectron microscopy with anti‐GFP antibodies to precisely assess their sub‐mitochondrial localization. MPP‐spHluorin immunoreactivity was preferentially detected in the matrix, while CIV8‐spHluorin gold particles were mainly detected in cristae, confirming their correct targeting (Fig (Fig3).3). MitopHlashes are therefore not associated with detectable flashing activity using pH probes verifiably located in IMS and cristae, suggesting that protons are not transferred across the mitochondrial inner membrane during flashes.

Figure 2
pH events are not detectable in the IMS or in the cristae
Figure EV3
Simultaneous Δψm and pH recordings in HeLa cells expressing DRP1K38A
Figure 3
Localization of CIV8‐ and MPP‐spHluorins in mitochondrial compartments

OPA1 modulates mitopHlash/Δψm coupling independently of mitochondrial fusion

We previously showed that OPA1 is necessary for flashing activity 28. To determine the contribution of OPA1 in this process, we performed concomitant measurements of mitopHlashes and Δψm in wild‐type (WT) and Opa1 −/− mouse embryonic fibroblasts (MEF) expressing mito‐sypHer. In WT cells, transient drops in TMRM fluorescence strictly coincided with mitopHlashes and 97% of depolarization transients were concomitant with mitopHlashes (Fig (Fig4A4A and E). Opa1 ablation caused a drastic loss in mitopHlashes while Δψm drops persisted unabatedly (Fig (Fig4B),4B), at a frequency similar to WT cells (0.9 ± 0.04 vs. 1.2 ± 0.43 depolarization/cell/min for WT and Opa1 −/−, Fig Fig4D).4D). As a result, the coupling between depolarization and pH transients dropped from 97% in WT cells to 4.4% in Opa1 −/− cells (Fig (Fig4E).4E). Re‐expression of WT OPA1 (variant 1) partially restored the coupling between mitopHlashes and Δψm drops to 34% (Fig (Fig4C4C and E).

Figure 4
OPA1 modulates mitopHlash/Δψm coupling

As the best characterized function of OPA1 is to mediate IMM fusion, we next investigated whether mitochondrial fusion is necessary to couple Δψm drops and mitopHlashes 28. The role of outer mitochondrial membrane (OMM) fusion was explored using cells lacking the two mitofusins (Mfn1/2 −/−) that coordinate OMM fusion and the role of IMM fusion by re‐expressing either WT OPA1 or the fusion‐deficient mutant OPA1K301A in Opa1 −/− cells 59. Monitoring the fusion of individual mitochondria by photoactivation of mito‐PA‐GFP confirmed that OPA1, but not OPA1K301A, restored mitochondrial fusion in Opa1 −/− cells (Fig (Fig5A5A and B). The frequencies of Δψm fluctuations were comparable in all conditions except in Opa1 −/− cells expressing OPA1K301A which exhibited a 3.7‐fold increase in TMRM spike frequency (Fig (Fig5C),5C), hinting to a stress condition. TMRM fluorescence images showing depolarizations of individual mitochondria in WT, Opa1 −/−cells transfected with a control plasmid, OPA1, or OPA1K301A are shown in Fig EV4. MitopHlashes were preserved in Mfn1/2 −/− cells and were equally restored by re‐expression of WT and OPA1K301A in Opa1 −/− cells (53% ± 7.8 vs. 63% ± 27.5 of TMRM spikes associated with mitopHlashes, respectively, Figs Figs5D5D and EV4). These results demonstrate that mitofusins, OMM fusion, and the fusogenic activity of OPA1 are dispensable for mitopHlash/Δψm coupling.

Figure 5
Mitochondrial fusion is not required for mitopHlashes
Figure EV4
Δψm recordings in MEF cells

Each long OPA1 variant can mediate mitopHlash/Δψm coupling without proteolytic processing

Fibroblasts express two splice variants of OPA1: variant 7 (V7), cleaved by the OMA1 and YME1L proteases at sites S1 and S2, respectively, and variant 1 (V1), cleaved only at S1 by OMA1 (Fig (Fig6A)6A) 21, 22, 23. Processing of these two variants leads to the production of three short soluble forms of OPA1 (S‐OPA1) that interact with the long, uncleaved forms of OPA1 (L‐OPA1) to form oligomeric complexes at cristae junctions (Fig (Fig1)1) 25, 26. To gain insight into the role of OPA1, we studied the individual contribution of the two splice variants in Δψm drops and mitopHlashes. We measured Δψm and matrix pH in Opa1 −/− cells expressing mito‐SypHer and either flag‐tagged V1 or V7. Each variant restored pH/Δψm coupling when expressed in Opa1 −/− cells, without altering the frequency of Δψm fluctuations (Fig (Fig6B6B and C), the variant 7 being slightly, but not significantly, more potent in restoring mitopHlashes (Fig (Fig6B).6B). To investigate the contribution of S‐OPA1, we used cells derived from Oma1 −/−, Yme1L −/−, or double Oma1 −/− Yme1L−/− knockout mice 27. Simultaneous recordings of membrane potential and pH flashes showed that the ablation of Oma1, Yme1L, or both genes had no effect on Δψm fluctuations and their coupling to mitopHlashes (Figs (Figs6D6D and E, and EV4). This indicates that OPA1 processing and thereby S‐OPA1 are dispensable for mitopHlash generation.

Figure 6
Each long OPA1 variant can mediate mitopHlash/Δψm coupling without proteolytic processing

mitopHlash/Δψm coupling is lost early during STS‐induced apoptosis

During apoptosis, destabilization of OPA1 high‐molecular‐weight complexes leads to a conformational change of cristae 10, 25, 26, a process associated with cytosolic release of OPA1 and cytochrome c 25. As the coupling between Δψm drops and mitopHlashes is mediated by OPA1, we hypothesized that pH/Δψm coupling would be lost in apoptotic cells. To test this possibility, we recorded matrix pH and Δψm fluctuations in cells exposed to staurosporine (STS, 1 μM). We observed a fivefold increase in depolarization frequency 2 h after STS addition compared to the DMSO control condition (Fig (Fig7A).7A). The increased Δψm flickering was followed by a complete collapse of Δψm in most cells after 3 h of STS exposure (data not shown). STS also triggered a significant uncoupling between Δψm and pH and only 37% of Δψm drops were associated with mitopHlashes 2 h after drug exposure compared to 92% in control conditions (Fig (Fig7B).7B). The STS‐induced pH/Δψm uncoupling was observed in cells depolarizing at both low and high frequency and was not associated with a change in matrix pH reported with mito‐SypHer (average pH of 7.54 ± 0.09 and 7.44 ± 0.02 after 2 h of DMSO and STS treatment, respectively). To investigate whether pH/Δψm uncoupling is a general feature of apoptosis, we also recorded pH and Δψm fluctuations over time in MEF cells exposed to 250 mM H2O2 or 100 μM etoposide (Fig EV5). These two death triggers had the same effect as STS, suggesting that the loss of coupling between Δψm drops and mitopHlashes is a conserved event during apoptosis. Western blot and immunofluorescence analyses showed that pH/Δψm uncoupling occurs before cytosolic release of OPA1 and cytochrome c (Fig (Fig7C7C and D), implying that an increased depolarization frequency and a loss of pH/Δψm coupling are early indicators of STS‐induced apoptosis. As OPA1 overexpression was shown to delay the apoptotic process and cristae remodeling 25, 60, we tested whether pH/Δψm uncoupling could be mitigated by enforced OPA1 expression. Simultaneous recordings of Δψm and pH in MEF cells exposed to STS for 2 h revealed that OPA1 overexpression significantly reduces pH/Δψm uncoupling (Fig (Fig7E).7E). The fraction of cells displaying a low pH/Δψm coupling (0 to 25%) dropped from 33.69 to 9.3% when OPA1 was overexpressed while the fraction of highly coupled cells (75 to 100%) increased from 25 to 48.84%. These data indicate that the uncoupling of mitopHlashes from Δψm fluctuations is an early marker of apoptosis that is dependent on the levels of OPA1, suggesting that the cristae‐shaping function of OPA1 contributes to mitopHlash/Δψm coupling.

Figure 7
mitopHlash/Δψm uncoupling occurs early during apoptosis and is prevented by OPA1 overexpression
Figure EV5
mitopHlash/Δψm uncoupling in cells exposed to H2O2 and etoposide


In this study, we confirm that mitoflashes are matrix alkalinization events and provide new mechanistic insight on this bioenergetic phenomenon. We recorded prototypical mitoflashes with three structurally distinct matrix‐targeted pH‐sensitive proteins (pHred, spHluorin, and SypHer), thereby establishing the pH nature of these events, which we renamed “mitopHlashes”. We then attempted to track the flow of protons across the mitochondrial respiratory membrane but failed to detect any pH flashing activity in cristae or the IMS. The resting pH reported by cristae probes was not modified by respiratory chain inhibitors and was more alkaline than previously reported 56, despite cristae probe localization verified by electron microscopy. This raises the possibility that mitopHlashes are confined within the matrix and not associated with proton pumping by respiratory complexes as previously postulated. Alternatively, the association of respiratory complexes into supercomplexes to optimize ATP synthesis could direct the flux of protons into a specific circuit that bypasses the pH sensors fused to the CIV and the CV. MitopHlashes correlate with cellular stress and organism life span, and their underlying molecular mechanism is the object of intense research. We previously reported that Opa1 ablation abrogates mitopHlash activity recorded with mito‐SypHer and proposed that the OPA1‐mediated formation of a fusion pore between adjacent mitochondria could equilibrate their potential, leading to compensatory mitopHlash responses 28. We show here with combined Δψm and pH measurements that the frequency of Δψm fluctuations is unaffected in Opa1 −/− cells, indicating that OPA1 is not necessary for the depolarization that triggers the mitopHlash response. Using TMRM spikes as indicators of activity, we could then detect residual mitopHlashes events in Opa1 −/− cells, indicating that Opa1 ablation severely impairs, but does not abrogate mitopHlash activity. OPA1 is therefore neither required for the generation of the electrical events nor for their conversion into a chemical response, but modulates the efficiency of pH/Δψm coupling. We also extend our previous findings linking the spatial spread of mitopHlashes to mitochondrial connectivity 28 by showing that mitopHlashes persist in Mfn1/2 −/− cells and are restored in Opa1 −/− cells by the re‐expression of a fusion‐deficient OPA1K301A mutant. These observations show that MFN‐mediated OMM fusion and OPA1‐mediated IMM fusion are dispensable for pH/Δψm coupling.

Picard et al 61 recently reported that adjacent mitochondria can coordinate their metabolic state by forming intermitochondrial junctions characterized by parallel alignments of their cristae. This trans‐mitochondrial communication could enhance bioenergetic efficiency across functional clusters of mitochondria during high energy demand and could provide the structural basis for rapid electrochemical events along connected mitochondria. In support for this, we previously reported that a high proportion (66%) of mitopHlashes bears adjacent mitochondria that coincidentally hyperpolarize 28, suggesting that mitopHlashes are associated with specific intermitochondrial contact sites. Correlative light‐electron microscopy will be required to unravel the ultrastructure of flashing mitochondria and determine whether specific mitochondrial or cristae arrangements are associated with mitopHlash activity.

OPA1 is known to maintain cristae shape via the formation of oligomers which assemble at cristae junctions to staple cristae membranes and tighten cristae necks 25, 26. In Yme1L −/− Oma1 −/− cells that do not generate S‐OPA1, cristae morphology remains unaltered 27, suggesting that L‐OPA1 is sufficient to form oligomers that sustain cristae architecture. During STS and t‐Bid‐induced apoptosis, OPA1 oligomers are disrupted as cristae are remodeled 25, 26. We observed that the coupling between Δψm and mitopHlashes was significantly decreased upon addition of different apoptotic triggers, consistent with a role of OPA1 oligomers in maintaining a permissive cristae architecture. Our observations that mitopHlashes persist in cells lacking OPA1 proteolytic processing and that each long OPA1 variant individually reconstitutes mitopHlashes in Opa1 −/− cells indicate that the formation of S‐OPA1 and L‐OPA1 hetero‐oligomers is not required for mitopHlashes. These data also show that S‐OPA1 variants known to co‐localize with the mitochondrial fission machinery 27 and to interact with subunits of the cristae‐organizing MICOS complex 24 are dispensable for flashing.

During apoptosis, cristae remodeling is associated with the disassembly of RCS, whose formation and stability also depends on OPA1 10. RCS disassembly decreases respiratory efficiency and might impair the ability of mitochondria to respond to Δψm fluctuations. RCS disassembly could thus account for the loss of pH/Δψm coupling upon Opa1 ablation and STS‐induced apoptosis. Conversely, OPA1 oligomerization during starvation leads to a tightening of cristae junctions and an increased ATP production by oxidative phosphorylation 60. As mitopHlashes occur in immobile mitochondria 44 that are found at sites of high ATP demand 62, 63, we speculate that OPA1 oligomers maintain flashing mitochondria in a high energetic state by enhancing RCS assembly, which in turn couple mitopHlashes to Δψm fluctuations.

In summary, we confirm that mitopHlashes are matrix‐limited pH responses to Δψm drops and show that this metabolic pH/Δψm coupling disappears early during apoptosis and requires long forms of OPA1 but not mitochondrial fusion. One possible role of OPA1 during mitopHlash biogenesis could be the stabilization of RCS to allow explosive proton pumping by the respiratory chain to compensate drops in membrane potential.

Materials and Methods

Cell lines, transfection, and media

HeLa cells were cultured in modified Eagle's medium (MEM) + glutamax (Gibco, 41090‐028). WT, Opa1 −/−, Mfn1/2 −/−, Oma1 −/−, Yme1L −/−, and Oma1 −/− Yme1L −/− MEFs were grown in Dulbecco's modified Eagle's medium (DMEM) (Gibco, 22320‐022). All media contained 10% fetal bovine serum (FBS), 1% penicillin and 1% streptomycin. For fluorescence imaging, cells were seeded and grown on 25‐mm glass cover slips and transfected with 5 μl Lipofectamine 2000 (Invitrogen) and 2 or 6 μg DNA for expression of fluorescent or non‐fluorescent proteins, respectively. During imaging, cells were incubated in a solution containing 20 mM N‐2‐hydroxyethylpiperazine‐N′‐2‐ethanesulfonic acid (HEPES), 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 2 mM CaCl2, 10 mM glucose, pH set to 7.4 at 37°C. To assess the mitochondrial localization of pH sensors, 5 μM antimycin A (Sigma) or 5 μg/ml oligomycin (Calbiochem) was added to the imaging media. High glucose medium was replaced with galactose or low glucose medium when indicated using DMEM without glucose (Gibco, 11966‐025) supplemented with 10 mM galactose (Sigma) or 5.5 mM glucose (sigma), 10% FBS, sodium pyruvate (Sigma, S‐8636), 3.7 g/l sodium bicarbonate, 1 mM sodium pyruvate, 2 mM glutamine, 1% penicillin, 1% streptomycin, and 25 mM HEPES for 2 days.

pH sensors used in the study

Mito‐sypHer, Cox8‐pHred, CVe‐, and CIV8‐spHluorins were already characterized 50, 55, 56, spHluorin being a derivate of GFP (F64L/S65T/S147D/N149Q/V163A/S175G/S202F/Q204T/A206T 51). MPP‐ and CV[Latin small letter gamma]‐spHluorins were a kind gift from K. Busch (Rieger B et al, in preparation). The subunit e of the CV and MPP was fused with their C‐termini to the N‐terminus of spHluorin. Smac‐rpHluorin was constructed by PCR amplification of the mitochondrial localization sequence of Smac (60 amino acids) using the primers 5′‐GAGAGCTAGCATGGCGGCTCTGAAGAG‐3′ (forward) and 5′‐GGGGGGATCCTACTCCAAAGCCAATC‐3′ (reverse) and the template vector pCi‐SmacMTS‐GFP‐AviTag‐Myc. It was then inserted into a pHluorin‐N1 vector encoding the GFP‐derived ratiometric pHluorin (S202H, E132D, S147E, N149L, N164I, K166Q, I167V, R168H, and L220F 51). Smac was fused to the N‐terminal extremity of rpHluorin.

Co‐localization between Hsp60 and pH sensors

HeLa cells expressing spHluorin, rpHluorin, or Cox8‐pHred were fixed to coverslips with PBS containing 3% paraformaldehyde for 15 min and permeabilized with 0.5% Triton X‐100 for 10 min. After blocking the coverslips in PBS‐2% bovine serum albumin (BSA) Hsp60 was stained using a mouse monoclonal antibody (Abcam, ab46798 diluted 1:200, overnight at 4°C) and a secondary goat anti‐mouse antibody conjugated to Alexa 594 (Molecular Probes, diluted 1:1,000, 1 h). Finally, coverslips were mounted in fluorescence mounting medium (Prolong, ThermoFisher) and analyzed with a Nikon A1R confocal microscope. The Pearson and Mander's coefficients were obtained using the Imaris software.

pH calibration and determination of resting pHmito

For pH titration, Cox8‐pHred was alternately excited for 800 ms at 430 and 560 nm through ET430/24× and 560AF55 filters and imaged with a 645AF75 band pass filter (Omega Optical). spHluorin and rpHluorin were excited for 700 ms at 488 nm or at 405 and 488 nm, respectively, through 380AF15, and 480BP10 filters (Omega Optical) and imaged with a 535AF26 band pass filter. This was performed at 37°C on an Axio Observer microscope (Zeiss, Germany) equipped with a Lambda DG4 illumination system (Sutter Instrument Company, Novato, CA, USA). Images were typically acquired every 5 s. Image acquisition and analysis were performed with MetaFluor 6.3 software (Universal Imaging, West Chester, PA). Mitochondrial pH was calibrated using nigericin (5 μg/ml) and monensin (5 μm) in 125 mm KCl, 20 mm NaCl, 0.5 mm MgCl2, 0.2 mm EGTA, and 20 mm N‐methyl‐D‐glutamine (pH 9.5–10.0), Tris (pH 8.0, 9.0), HEPES (pH 7.0–7.5), or MES (pH 5.5–6.5). For each cell, a six‐point calibration curve was fitted to a variable slope sigmoid equation using GraphPad Prism, and the resting pHmito was determined by reporting the initial fluorescence values to the calibration curves.

Ratiometric changes of Cox8‐pHred fluorescence and concomitant pH and potential measurements

Images were acquired on a Nikon a1r inverted confocal microscope with a ×60 objective (oil; CFI Plan APO 1.4 NA) and typically one image was acquired every 1 s. Rapid ratiometric changes of fluorescence measured with Cox8‐pHred were recorded using two excitation wavelengths (405 and 561 nm) and one 624/40 emission filter. Time‐resolved pH and potential imaging was performed at 37°C on cells transiently transfected with mito‐sypHer or pHluorins and loaded with 4 or 20 nM TMRM for HeLa and MEF cells, respectively. Images were acquired using 488‐ and 561‐nm excitations and 520/35 and 624/40 emission filters. The frequency of depolarizations and pH flashes was analyzed using ImageJ.

Mitochondrial fusion activity

WT MEFs and Opa1 −/− cells transfected with 6 μg pCXN2‐OPA1 or OPA1K301A (gift from Prof. Yoshihiro Kubo) and 1 μg mito‐PA‐GFP and 0.5 μg mito‐DsRed were imaged in DMEM media. Cells with low GFP fluorescence intensity were selected to avoid saturation of the GFP emission upon photoactivation 62. GFP and mito‐DsRed were imaged concurrently on a Nikon A1R confocal microscope with a × 60 objective (oil; CFI Plan APO 1.4 NA) using 488 and 561 nm excitation and 520/35 and 624/40 emission filters. Three images were acquired (one image/second) before applying three stimulation pulses (500 ms, 405 nm laser 50 mW, 7% power) followed by live imaging. Loss of focus was minimized by using the Perfect Focus system (Nikon). ImageJ was used for data analysis of the area of PA‐GFP. Fusion was quantified as the % of PA‐GFP area increase after 60 min compared to the initial PA‐GFP area measured 1 min post‐photoactivation in the same cell.

Electron microscopy

For immunoelectron microscopy, cells were fixed with 2% paraformaldehyde and 0.2% glutaraldehyde and processed for cryo‐ultramicrotomy as described in 64. Ultrathin frozen sections were prepared and incubated for immunolabeling as described in 65. Rabbit polyclonal anti‐GFP (Abcam, 1:200) were used as primary antibodies and goat anti‐rabbit IgG gold as secondary antibodies (gold size, 15 nm). The analysis of gold particle localization was performed using the image analysis software Leica QWin Standard (Leica Imaging Systems Ltd.) and a Wacom graphic‐pen tablet on electron micrographs at the final magnification ×93,500.

Cytosolic release of OPA1 and cytochrome c

Mouse embryonic fibroblasts cells were treated with 1 μM STS for 2, 6, or 8 h or with DMSO for 6 or 8 h. Release of OPA1 in the cytoplasm was determined by Western blot after cytosolic fractionation. Cells were permeabilized with 8 mg/ml digitonin for 10 min and centrifuged at 10,000 g for 10 min. The supernatants were boiled at 99°C for 5 min prior to separation of proteins by SDS–PAGE. Proteins were transferred electrophoretically onto nitrocellulose membranes which were blocked for 1 h with TBS‐0.05% Tween containing 5% non‐fat dried milk at room temperature. Membranes were then incubated with mouse monoclonal anti‐OPA1 (BD Transduction Laboratories, clone 18/OPA1 diluted 1:1,000) and mouse monoclonal anti‐tubulin (Sigma, clone DM1A diluted 1:2,000). Peroxidase‐conjugated anti‐mouse sera were used as secondary antibodies (Bio‐Rad, diluted 1:5,000). Detection was carried out using a chemiluminescence detection kit (Immobilon western, Millipore), and the bands were detected using Syngene PXi (Syngene) and the software Genesys V1.5.00. The amounts of cytosolic OPA1 and tubulin were quantified using ImageJ, and the level of OPA1 release was expressed as the ratio of OPA1/tubulin.

Cytochrome c release was assessed by immunofluorescence. MEF cells expressing mito‐DsRed were fixed and permeabilized. After blocking the coverslips, cytochrome c was stained using a mouse monoclonal antibody (BD Pharmingen, clone 6H2.B4 diluted 1:200, overnight at 4°C) and a secondary goat anti‐mouse antibody conjugated to Alexa 488 (Molecular Probes, diluted 1:1,000, 1 h). The nucleus was stained with 4′,6‐diamidino‐2‐phenylindol (DAPI) for 30 min (Sigma). Finally, coverslips were mounted in fluorescence mounting medium (Prolong, ThermoFisher) and analyzed with a Nikon A1R confocal microscope. The number of cells releasing cytochrome c was determined using ImageJ.

Statistical analysis

All statistical analyses were performed using GraphPad Prism version 6.05. Significance between three or more group sets was analyzed using one‐way ANOVA. To analyze the statistical differences among flash frequencies and pH/Δψm coupling using STS and DMSO at different time points and among the distribution of MPP‐ and CIV8‐spHluorins in the different cellular sublocalizations, a two‐way ANOVA was performed. Statistical differences of pH/Δψm coupling in WT MEFs transfected with a control plasmid or OPA1 and of resting pH values in HeLa cells cultured in galactose or low glucose media were analyzed using an unpaired t‐test with Welch's correction. The statistical test used to analyze OPA1 cytosolic release by Western blot is a ratio paired t‐test. ns: non‐significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Author contributions

MR and ND designed experiments. MR and FB performed experiments and MR analyzed data. JS‐D generated the pH sensor Smac‐rpHluorin. MR, JS‐D, MG, and ND drafted the article or revised it critically for important intellectual content.

Conflict of interest

The authors declare that they have no conflict of interest.

Supporting information

Expanded View Figures PDF

Review Process File


We thank Mr. Cyril Castelbou for expert technical assistance, Ms. Monica Bulla, Dr. Paula Nunes, Dr. Denis Martinvalet, and Prof. Claes Wolheim for their helpful comments. We also thank Prof. Jean‐Claude Martinou for providing constructs encoding OPA1‐flagged variants, Prof. Yoshihiro Kubo for pCXN2‐OPA1 constructs, and Prof. Karin Busch for MPP‐, CV[Latin small letter gamma]‐, CVe‐ and CIV8‐spHluorins. Oma1 −/−, Yme1L −/−, and Oma1 −/− Yme1L −/− cells were a kind gift from Prof. Thomas Langer and Mfn1/2 −/− MEF cells from Prof. David Chan. We also thank Pilar Ruga Fahy for her assistance in preparing electron microscopy samples. This work was supported by the Swiss National Science Foundation grant 31003A‐149566.


EMBO Reports (2017) 18: 451–463


1. Hatefi Y (1985) The mitochondrial electron transport and oxidative phosphorylation system. Annu Rev Biochem 54: 1015–1069 [PubMed]
2. Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi‐osmotic type of mechanism. Nature 191: 144–148 [PubMed]
3. Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc 41: 445–502 [PubMed]
4. Nicholls DG (1974) The influence of respiration and ATP hydrolysis on the proton‐electrochemical gradient across the inner membrane of rat‐liver mitochondria as determined by ion distribution. Eur J Biochem 50: 305–315 [PubMed]
5. Rottenberg H (1975) The measurement of transmembrane electrochemical proton gradients. J Bioenerg 7: 61–74 [PubMed]
6. Mitchell P (1975) Proton translocation mechanisms and energy transduction by adenosine triphosphatases: an answer to criticisms. FEBS Lett 50: 95–97 [PubMed]
7. Eubel H, Heinemeyer J, Sunderhaus S, Braun HP (2004) Respiratory chain supercomplexes in plant mitochondria. Plant Physiol Biochem 42: 937–942 [PubMed]
8. Hatefi Y, Rieske JS (1967) [41] The preparation and properties of DPNH‐cytochrome c reductase (complex I‐III of the respiratory chain) In Methods in enzymology, Vol 10, 6th edn, pp 225–231. New York, NY: Academic Press;
9. Schagger H, Pfeiffer K (2000) Supercomplexes in the respiratory chains of yeast and mammalian mitochondria. EMBO J 19: 1777–1783 [PubMed]
10. Cogliati S, Frezza C, Soriano ME, Varanita T, Quintana‐Cabrera R, Corrado M, Cipolat S, Costa V, Casarin A, Gomes LC et al (2013) Mitochondrial cristae shape determines respiratory chain supercomplexes assembly and respiratory efficiency. Cell 155: 160–171 [PubMed]
11. Olichon A, Baricault L, Gas N, Guillou E, Valette A, Belenguer P, Lenaers G (2003) Loss of OPA1 perturbates the mitochondrial inner membrane structure and integrity, leading to cytochrome c release and apoptosis. J Biol Chem 278: 7743–7746 [PubMed]
12. Chen H, Chomyn A, Chan DC (2005) Disruption of fusion results in mitochondrial heterogeneity and dysfunction. J Biol Chem 280: 26185–26192 [PubMed]
13. Cipolat S, de Brito OM, Dal Zilio B, Scorrano L (2004) OPA1 requires mitofusin 1 to promote mitochondrial fusion. Proc Natl Acad Sci USA 101: 15927–15932 [PubMed]
14. Song Z, Chen H, Fiket M, Alexander C, Chan DC (2007) OPA1 processing controls mitochondrial fusion and is regulated by mRNA splicing, membrane potential, and Yme1L. J Cell Biol 178: 749–755 [PubMed]
15. Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC (2003) Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J Cell Biol 160: 189–200 [PubMed]
16. Ishihara N, Eura Y, Mihara K (2004) Mitofusin 1 and 2 play distinct roles in mitochondrial fusion reactions via GTPase activity. J Cell Sci 117: 6535–6546 [PubMed]
17. Koshiba T, Detmer SA, Kaiser JT, Chen H, McCaffery JM, Chan DC (2004) Structural basis of mitochondrial tethering by mitofusin complexes. Science 305: 858–862 [PubMed]
18. Delettre C, Griffoin J‐M, Kaplan J, Dollfus H, Lorenz B, Faivre L, Lenaers G, Belenguer P, Hamel CP (2001) Mutation spectrum and splicing variants in the OPA1 gene. Hum Genet 109: 584–591 [PubMed]
19. Delettre C, Lenaers G, Belenguer P, Hamel CP (2003) Gene structure and chromosomal localization of mouse Opa1: its exclusion from the Bst locus. BMC Genet 4: 8 [PubMed]
20. Olichon A, Elachouri G, Baricault L, Delettre C, Belenguer P, Lenaers G (2007) OPA1 alternate splicing uncouples an evolutionary conserved function in mitochondrial fusion from a vertebrate restricted function in apoptosis. Cell Death Differ 14: 682–692 [PubMed]
21. Ehses S, Raschke I, Mancuso G, Bernacchia A, Geimer S, Tondera D, Martinou J‐C, Westermann B, Rugarli EI, Langer T (2009) Regulation of OPA1 processing and mitochondrial fusion by m‐AAA protease isoenzymes and OMA1. J Cell Biol 187: 1023–1036 [PubMed]
22. Head B, Griparic L, Amiri M, Gandre‐Babbe S, van der Bliek AM (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells. J Cell Biol 187: 959–966 [PubMed]
23. Ishihara N, Fujita Y, Oka T, Mihara K (2006) Regulation of mitochondrial morphology through proteolytic cleavage of OPA1. EMBO J 25: 2966–2977 [PubMed]
24. Ding C, Wu Z, Huang L, Wang Y, Xue J, Chen S, Deng Z, Wang L, Song Z, Chen S (2015) Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Sci Rep 5: 16064 [PubMed]
25. Frezza C, Cipolat S, Martins de Brito O, Micaroni M, Beznoussenko GV, Rudka T, Bartoli D, Polishuck RS, Danial NN, De Strooper B et al (2006) OPA1 controls apoptotic cristae remodeling independently from mitochondrial fusion. Cell 126: 177–189 [PubMed]
26. Yamaguchi R, Lartigue L, Perkins G, Scott RT, Dixit A, Kushnareva Y, Kuwana T, Ellisman MH, Newmeyer DD (2008) Opa1‐mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization. Mol Cell 31: 557–569 [PubMed]
27. Anand R, Wai T, Baker MJ, Kladt N, Schauss AC, Rugarli E, Langer T (2014) The i‐AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. J Cell Biol 204: 919–929 [PubMed]
28. Santo‐Domingo J, Giacomello M, Poburko D, Scorrano L, Demaurex N (2013) OPA1 promotes pH flashes that spread between contiguous mitochondria without matrix protein exchange. EMBO J 32: 1927–1940 [PubMed]
29. Wang W, Fang H, Groom L, Cheng A, Zhang W, Liu J, Wang X, Li K, Han P, Zheng M et al (2008) Superoxide flashes in single mitochondria. Cell 134: 279–290 [PubMed]
30. Azarias G, Chatton JY (2011) Selective ion changes during spontaneous mitochondrial transients in intact astrocytes. PLoS One 6: e28505 [PubMed]
31. Schwarzlander M, Logan DC, Fricker MD, Sweetlove LJ (2011) The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide ‘flashes’. Biochem J 437: 381–387 [PubMed]
32. Schwarzländer M, Logan DC, Johnston IG, Jones NS, Meyer AJ, Fricker MD, Sweetlove LJ (2012) Pulsing of membrane potential in individual mitochondria: a stress‐induced mechanism to regulate respiratory bioenergetics in Arabidopsis [W]. Plant Cell 24: 1188–1201 [PubMed]
33. Schwarzlander M, Wagner S, Ermakova YG, Belousov VV, Radi R, Beckman JS, Buettner GR, Demaurex N, Duchen MR, Forman HJ et al (2014) The ‘mitoflash’ probe cpYFP does not respond to superoxide. Nature 514: E12–E14 [PubMed]
34. Pouvreau S (2010) Superoxide flashes in mouse skeletal muscle are produced by discrete arrays of active mitochondria operating coherently. PLoS One 5: e13035 [PubMed]
35. Wei L, Salahura G, Boncompagni S, Kasischke KA, Protasi F, Sheu SS, Dirksen RT (2011) Mitochondrial superoxide flashes: metabolic biomarkers of skeletal muscle activity and disease. FASEB J 25: 3068–3078 [PubMed]
36. De Giorgi F, Lartigue L, Ichas F (2000) Electrical coupling and plasticity of the mitochondrial network. Cell Calcium 28: 365–370 [PubMed]
37. Duchen MR, Leyssens A, Crompton M (1998) Transient mitochondrial depolarizations reflect focal sarcoplasmic reticular calcium release in single rat cardiomyocytes. J Cell Biol 142: 975–988 [PubMed]
38. Huser J, Blatter LA (1999) Fluctuations in mitochondrial membrane potential caused by repetitive gating of the permeability transition pore. Biochem J 343: 311–317 [PubMed]
39. Huser J, Rechenmacher CE, Blatter LA (1998) Imaging the permeability pore transition in single mitochondria. Biophys J 74: 2129–2137 [PubMed]
40. Jacobson J, Duchen MR (2002) Mitochondrial oxidative stress and cell death in astrocytes–requirement for stored Ca2+ and sustained opening of the permeability transition pore. J Cell Sci 115: 1175–1188 [PubMed]
41. Zorov DB, Filburn CR, Klotz LO, Zweier JL, Sollott SJ (2000) Reactive oxygen species (ROS)‐induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes. J Exp Med 192: 1001–1014 [PubMed]
42. Thiffault C, Bennett JP Jr (2005) Cyclical mitochondrial deltapsiM fluctuations linked to electron transport, F0F1 ATP‐synthase and mitochondrial Na+/Ca+2 exchange are reduced in Alzheimer's disease cybrids. Mitochondrion 5: 109–119 [PubMed]
43. Buckman JF, Reynolds IJ (2001) Spontaneous changes in mitochondrial membrane potential in cultured neurons. J Neurosci 21: 5054–5065 [PubMed]
44. Fang H, Chen M, Ding Y, Shang W, Xu J, Zhang X, Zhang W, Li K, Xiao Y, Gao F et al (2011) Imaging superoxide flash and metabolism‐coupled mitochondrial permeability transition in living animals. Cell Res 21: 1295–1304 [PubMed]
45. Hou T, Zhang X, Xu J, Jian C, Huang Z, Ye T, Hu K, Zheng M, Gao F, Wang X et al (2013) Synergistic triggering of superoxide flashes by mitochondrial Ca2+ uniport and basal reactive oxygen species elevation. J Biol Chem 288: 4602–4612 [PubMed]
46. Ma Q, Fang H, Shang W, Liu L, Xu Z, Ye T, Wang X, Zheng M, Chen Q, Cheng H (2011) Superoxide flashes: early mitochondrial signals for oxidative stress‐induced apoptosis. J Biol Chem 286: 27573–27581 [PubMed]
47. Shen EZ, Song CQ, Lin Y, Zhang WH, Su PF, Liu WY, Zhang P, Xu J, Lin N, Zhan C et al (2014) Mitoflash frequency in early adulthood predicts lifespan in Caenorhabditis elegans . Nature 508: 128–132 [PubMed]
48. Quatresous E, Legrand C, Pouvreau S (2012) Mitochondria‐targeted cpYFP: pH or superoxide sensor? J Gen Physiol 140: 567–570 [PubMed]
49. Wei‐LaPierre L, Gong G, Gerstner BJ, Ducreux S, Yule DI, Pouvreau S, Wang X, Sheu SS, Cheng H, Dirksen RT et al (2013) Respective contribution of mitochondrial superoxide and pH to mitochondria‐targeted circularly permuted yellow fluorescent protein (mt‐cpYFP) flash activity. J Biol Chem 288: 10567–10577 [PubMed]
50. Tantama M, Hung YP, Yellen G (2011) Imaging intracellular pH in live cells with a genetically encoded red fluorescent protein sensor. J Am Chem Soc 133: 10034–10037 [PubMed]
51. Miesenbock G, De Angelis DA, Rothman JE (1998) Visualizing secretion and synaptic transmission with pH‐sensitive green fluorescent proteins. Nature 394: 192–195 [PubMed]
52. Abad MFC, Di Benedetto G, Magalhães PJ, Filippin L, Pozzan T (2004) Mitochondrial pH monitored by a new engineered green fluorescent protein mutant. J Biol Chem 279: 11521–11529 [PubMed]
53. Bolshakov AP, Mikhailova MM, Szabadkai G, Pinelis VG, Brustovetsky N, Rizzuto R, Khodorov BI (2008) Measurements of mitochondrial pH in cultured cortical neurons clarify contribution of mitochondrial pore to the mechanism of glutamate‐induced delayed Ca2+ deregulation. Cell Calcium 43: 602–614 [PubMed]
54. Matsuyama S, Llopis J, Deveraux QL, Tsien RY, Reed JC (2000) Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis. Nat Cell Biol 2: 318–325 [PubMed]
55. Poburko D, Santo‐Domingo J, Demaurex N (2011) Dynamic regulation of the mitochondrial proton gradient during cytosolic calcium elevations. J Biol Chem 286: 11672–11684 [PubMed]
56. Rieger B, Junge W, Busch KB (2014) Lateral pH gradient between OXPHOS complex IV and F(0)F(1) ATP‐synthase in folded mitochondrial membranes. Nat Commun 5: 3103 [PubMed]
57. Pitts KR, Yoon Y, Krueger EW, McNiven MA (1999) The dynamin‐like protein DLP1 is essential for normal distribution and morphology of the endoplasmic reticulum and mitochondria in mammalian cells. Mol Biol Cell 10: 4403–4417 [PubMed]
58. Santel A, Fuller MT (2001) Control of mitochondrial morphology by a human mitofusin. J Cell Sci 114: 867–874 [PubMed]
59. Misaka T, Murate M, Fujimoto K, Kubo Y (2006) The dynamin‐related mouse mitochondrial GTPase OPA1 alters the structure of the mitochondrial inner membrane when exogenously introduced into COS‐7 cells. Neurosci Res 55: 123–133 [PubMed]
60. Patten DA, Wong J, Khacho M, Soubannier V, Mailloux RJ, Pilon‐Larose K, MacLaurin JG, Park DS, McBride HM, Trinkle‐Mulcahy L et al (2014) OPA1‐dependent cristae modulation is essential for cellular adaptation to metabolic demand. EMBO J 33: 2676–2691 [PubMed]
61. Picard M, McManus MJ, Csordas G, Varnai P, Dorn GW 2nd, Williams D, Hajnoczky G, Wallace DC (2015) Trans‐mitochondrial coordination of cristae at regulated membrane junctions. Nat Commun 6: 6259 [PubMed]
62. Cai Q, Davis ML, Sheng ZH (2011) Regulation of axonal mitochondrial transport and its impact on synaptic transmission. Neurosci Res 70: 9–15 [PubMed]
63. Hollenbeck PJ (1996) The pattern and mechanism of mitochondrial transport in axons. Front Biosci 1: d91–d102 [PubMed]
64. Nunes P, Cornut D, Bochet V, Hasler U, Oh‐Hora M, Waldburger J‐M, Demaurex N (2012) STIM1 juxtaposes ER to phagosomes, generating Ca2+ hotspots that boost phagocytosis. Curr Biol 22: 1990–1997 [PubMed]
65. Liou W, Geuze HJ, Slot JW (1996) Improving structural integrity of cryosections for immunogold labeling. Histochem Cell Biol 106: 41–58 [PubMed]

Articles from EMBO Reports are provided here courtesy of The European Molecular Biology Organization