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Exogenous insulin use may interfere with the T helper cells’ cytokine production. This dataset presents the relationship between pre-existing use of injectable insulin in women diagnosed with breast cancer and type 2 diabetes mellitus, the T-helper 1 and 2 produced cytokine profiles at the time of breast cancer diagnosis, and subsequent cancer outcomes. A Pearson correlation analysis evaluating the relationship between T-helper cytokines stratified by of insulin use and controls is also provided.
Value of the data
Reported data represents the observed association between pre-existing use of injectable insulin before breast cancer diagnosis and the T-helper 1 and 2 produced cytokine profiles upon cancer diagnosis in women with both breast cancer and diabetes mellitus (Table 1). Data in Table 2 includes the observed correlations between T-helper 1 and 2 cytokines stratified by diabetes mellitus pharmacotherapy and controls.
Evaluation of the association between profiles of T-helper 1 and 2 produced cytokines, injectable insulin use and BC outcomes was carried out under two protocols approved by both Roswell Park Cancer Institute (EDR154409 and NHR009010) and the State University of New York at Buffalo (PHP0840409E). Demographic and clinical patient information was linked with cancer outcomes and profiles of T-helper 1 and 2 produced cytokines of corresponding plasma specimen harvested at BC diagnosis and banked in the Roswell Park Cancer Institute Data Bank and Bio-Repository.
All incident breast cancer cases diagnosed at Roswell Park Cancer Institute (01/01/2003-12/31/2009) were considered for inclusion (n=2194). Medical and pharmacotherapy history were used to determine the baseline presence of diabetes.
All adult women with pre-existing diabetes at breast cancer diagnosis having available banked treatment-naïve plasma specimens (blood collected prior to initiation of any cancer-related therapy - surgery, radiation or pharmacotherapy) in the Institute’s Data Bank and Bio-Repository were included.
Subjects were excluded if they had prior cancer history or unclear date of diagnosis, incomplete clinical records, type 1 or unclear diabetes status. For a specific breakdown of excluded subjects, please see the original research article by Wintrob et al. .
A total of 97 female subjects with breast cancer and baseline diabetes mellitus were eligible for inclusion in this analysis.
Each of the 97 adult female subjects with breast cancer and diabetes mellitus (defined as “cases”) was matched with two other female subjects diagnosed with breast cancer, but without baseline diabetes mellitus (defined as “controls”). The following matching criteria were used: age at diagnosis, body mass index category, ethnicity, menopausal status and tumor stage (as per the American Joint Committee on Cancer). Some matching limitations applied .
Clinical and treatment history was documented as previously described . Briefly, users of any insulin were defined as patients receiving a form of injectable insulin – alone or in combination – at the time of breast cancer diagnosis. Vital status was obtained from the Institute’s Tumor Registry, a database updated biannually with data obtained from the National Comprehensive Cancer Networks’ Oncology Outcomes Database. Outcomes of interest were breast cancer recurrence and/or death. Details regarding patient demographics and clinical characteristics have been previously published .
All the plasma specimens retrieved from long-term storage were individually aliquoted in color coded vials labeled with unique, subject specific barcodes. Overall duration of freezing time was accounted for all matched controls ensuring that the case and matched control specimens had similar overall storage conditions. Only two instances of freeze-thaw were allowed between biobank retrieval and biomarker analyses: aliquoting procedure step and actual assay.
A total of 12 biomarkers - interleukine-2, soluble interleukine-2 receptor α, interleukine-12 subunit p40, interleukine-12 subunit p70, interferon α 2, interferon γ, chemokine ligand 10 (interferon gamma-induced protein 10), chemokine ligand 9 (monokine-induced by interferon γ), chemokine ligand 8 (interleukine-8), interleukine-5, interleukine-10, and interleukine-13 - were quantified according to the manufacturer protocol. The Luminex® HCYTOMAG-60K panel (Millipore Corporation, Billerica, MA) was used in this study.
Biomarker cut-point optimization was performed for each analyzed biomarker. Biomarker levels constituted the continuous independent variable that was subdivided into two groups that optimized the log rank test among all possible cut-point selections yielding a minimum of 10 patients in any resulting group. Quartiles were also constructed. The resultant biomarker categories were then tested for association with type 2 diabetes mellitus therapy and controls by Fisher’s exact test. The continuous biomarker levels were also tested for association with diabetes therapy and controls across groups by the Kruskal-Wallis test and pairwise by the Wilcoxon rank sum. Multivariate adjustments were performed accounting for age, tumor stage, body mass index, estrogen receptor status, and cumulative comorbidity. The biomarker analysis was performed using R Version 2.15.3. Please see the original article for an illustration of the analysis workflow .
Correlations between biomarkers stratified by type 2 diabetes mellitus pharmacotherapy and controls were assessed by the Pearson method. Correlation models were constructed both with and without adjustment for age, body mass index, and the combined comorbidity index. Correlation analyses were performed using SAS Version 9.4.
This research was funded by the following grant awards: Wadsworth Foundation Peter Rowley Breast Cancer Grant awarded to A.C.C. (UB Grant number 55705, Contract CO26588).
Authors acknowledge the valuable help of Dr. Chi-Chen Hong with case-control matching.
Transparency documentSupplementary data associated with this article can be found in the online version at http://dx.doi.org/10.1016/j.dib.2017.02.028.