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Glioma cells grow in two phenotypic forms, as adherent monolayers and as free floating “neurospheres/tumorspheres”, using specific media supplements. Whether each phenotype is irreversible remains unknown. Herein we show that both states are reversible using patient derived glioblastoma cell cultures (i.e., HF2303, IN859, MGG8, IN2045). Both phenotypic states differ in proliferation rate, invasion, migration, chemotaxis and chemosensitivity. We used microarrays to characterize gene expression across the patient derived glioblastoma cell cultures, to find specific inhibitors of the sphere population. Traditional chemotherapeutics (i.e., doxorubicin or paclitaxel) inhibit rapidly dividing adherent cells; it has been more challenging to inhibit the growth of the sphere phenotype. PRKG1, known to induce apoptosis when activated, is increased in all patient derived glioblastoma spheres. Stimulation of PRKG1 activity preferentially reduced cell viability in the sphere phenotype. Computational network and gene ontology analysis identified novel potential target genes linked to the PRKG1 expression node.
Lack of a precise experimental definition of glioma stem cells has led to variance in the way these cells are cultured in vitro, characterized in in vitro assays, and to confusing nomenclature. A conceptual and practical definition of glioma stem cells exists and characterizes these cells by their ability to form tumors in vivo following implantation, extensive self-renewal, asymmetric division generating tumorigenic and non-tumorigenic cells, multilineage differentiation potential, and formation of neuro/tumorspheres in vitro. An exact experimental definition has been in flux. In 2003, Singh et al.,  published the first identification and purification of human brain tumor stem cells. They defined human brain tumor stem cells as being capable of forming neurospheres in serum-free medium, expressing markers of differentiation when grown adherently on L-poly-ornithine coated coverslips in fetal bovine serum supplemented medium, and being CD133+ . Subsequent to this report, the definition was expanded to include tumor growth when transplanted into rodent models [4,5]. This experimental definition was challenged when CD133- cells were shown to form tumor spheres in-vitro and tumors in-vivo [6–8]. So far no markers or phenotypic functions have been shown to be unequivocally pathognomonic of glioma stem cells, although a number have been tried and reported, including immunophenotyping for markers other than CD133, side population analysis, aptamer selection, and intrinsic autofluorescence [9–14]. Despite conflicting evidence, methods’ sections of many, yet not all papers, refer to CD133+ as the defining marker of glioma stem cells . Equally CD133 expression continues to be the cornerstone definition of glioma stem cells by some groups . Nevertheless, given contradictory evidence for CD133+ cells being the pathognomonic marker of glioma stem cells, there does not appear to exist one single universally accepted definition of glioma stem cells. In our experiments, as CD133 was expressed by cells of both the sphere and adherent phenotype, it could not be used as a differential marker.
Inconsistencies continue to abound in the literature. In many studies reporting on glioma stem cells, brain tumor propagating cells, brain tumor initiating cells, etc., it remains unclear which definition is being used. This makes replication, interpretation and generalization of these studies difficult. Some studies describe glioma stem cells in in vitro cultures as adherent monolayers [16–18], while others only accept neurosphere-like tumorspheres [3, 18, 19]. The significance of varied methods of culture remains to be clarified. For example, whether cells behave differently under these two culture conditions and whether the method of culture modifies the outcome of assays such as the testing of chemotherapeutics deserves attention. Equally, the identification of the sub-population of cells that function as stem cells in vivo remains unsolved. It is possible that clarification of the in vitro work will help address the in vivo challenges as well.
In response to these challenges, we propose a simple, reproducible experimental definition of glioma propagating/initiating cells (GPCs). To create an experimental definition of GPCs and a new method for the identification of potential therapeutic molecules, we focused on understanding the ramifications of the variance of the culture conditions. We hypothesized that GPCs would grow both as an adherent monolayer and as neurospheres but that their behavior would depend on phenotype and culture conditions, i.e., that the glioma stem cell state is dynamic and allows reversible switching between both states. We further hypothesized that comparing genetic expression profiles would inform us about the signaling mechanisms responsible any observed differences in proliferation, invasion, and chemoresistance. We tested these hypotheses, and found that all patient derived glioblastoma cell cultures tested can grow reversibly as an adherent monolayer and as tumorspheres. Each culture condition/phenotype, however, has different characteristics. For example, the adherent phenotype was characterized by higher proliferation, higher invasion, and lower resistance to chemotherapy in vitro. We wanted to utilize comparative genetic expression profiles in order to reveal potential therapeutic molecules in the chemoresistant sphere phenotype thought to cause recurrence of gliomas WHO IV. By identifying such molecules in the sphere population we hope to inhibit both the adherent proliferative population as well as the quiescent chemo-resistant sphere phenotype. cGMP-protein dependent kinase I (PRKG1) increases in spheres. This effect occurred in a cell-cycle independent fashion and was observed most notably in the subset of cells that were highly resistant to traditional chemotherapy. Thus, PRKG1 represents a novel therapeutic target to inhibit growth of glioma stem cells.
To test our hypotheses we sought to determine if the distinct growth patterns confer different behavioral patterns. We compared the proliferation rate of cells under the two conditions by MTS assay. Cells grown adherently demonstrated a higher proliferation rate than the same cells grown under sphere conditions for all four patient derived glioblastoma cell cultures tested (Figure (Figure1).1). CD133 was expressed by cells of both the sphere and adherent phenotype (Figure S1), which made it unsuitable to use as a differential marker for glioma stem cells.
We then tested cellular behavior using a Matrigel invasion assay to compare both the migration and invasion of cells of both adherent and sphere phenotypes. Within each patient derived glioblastoma cell culture, cells in the adherent phenotype displayed both higher migration towards FBS and higher invasion through a Matrigel matrix (Figure (Figure22).
Next, we studied the in vitro sensitivity to chemotherapeutic agents temozolomide, cisplatin, paclitaxel, and doxorubicin to compare the chemosensitivity of the adherent and sphere phenotypes. For all four patient derived glioblastoma cell cultures, temozolomide showed no effect in either the adherent or sphere phenotype, with the exception of a small effect in the IN2045 adherent phenotype (Figure (Figure3A).3A). Temozolomide was inactive in all other groups. Doxorubicin was cytotoxic for all patient derived glioblastoma cell cultures tested (Figure (Figure3B),3B), and the sphere phenotype was more resistant to doxorubicin. This difference can be explained by the cell cycle dependency of doxorubicin's cytotoxicity (Figure (Figure1).1). An effective response was seen for cisplatin and paclitaxel, two DNA damaging agents, for all four patient derived glioblastoma cell cultures and both phenotypes (Figure 3C-3D). HF2303 spheres were highly sensitive to paclitaxel. For all patient derived glioblastoma cell cultures and treatments the sphere phenotype was more resistant to chemotherapy than the adherent phenotype.
We hypothesized that, given the different in vitro behavior of these phenotypes, when implanted into the brain, they might behave distinctly. We tested this hypothesis by implanting glioma cells grown adherently or as spheres into the striatum and studying their in vivo growth. For IN859 cells, the survival was similar for both groups (Figure (Figure4A),4A), with mean survival being 65.8 days for the adherent cells and 65.5 days for the sphere cells. For the HF2303 cells, the survival was comparable (Figure (Figure4B)4B) for both groups with mean survival being 135 days for the adherent phenotype and 150 days for the sphere phenotype. In addition to having similar survival times, both IN859 tumor types showed similar morphology, as did the HF2303 tumors. IN859 tumors showed more homogeneous growth with well demarcated, non-invasive borders (Figure (Figure4C).4C). In contrast, HF2303 tumors showed more irregular growth with poorly demarcated, highly invasive borders (Figure (Figure4D4D).
Both phenotypes of glioma cells displayed similar in vivo behavior after being placed in the brain microenvironment and grown as tumors. As in vitro they display different behaviors, and in vivo they behave similarly, we hypothesized that the phenotypes (in vitro v. in vivo) are reversible. We tested this hypothesis by comparing the proliferation rate of sphere cells when placed into adherent medium with baseline growth of adherent and sphere cells in their corresponding medium (Figure 5A-5B). In this experiment sphere cells plated into adherent medium increased their proliferation, illustrating the capacity of sphere cells to become adherent cells (Figure (Figure5).5). Comparable phenotypic changes were seen for all cells in vitro. All patient derived glioblastoma cell cultures were shown to revert to a neurosphere morphology when placed into sphere medium, and into an adherent morphology when placed into the adherent medium (Figure (Figure66).
Our data so far suggested that the overall phenotypes (i.e., proliferation, invasion, chemotaxi, morphology, etc.) were reversible (i.e, cells could transition from spheres to adherent, and vice-versa). These data suggested that the expression of individual mRNAs should also differ, and be reversible between phenotypes. To test this we performed qPCR on a selected panel of mRNAs of importance in glioma stem cell function: CD133, nestin, vimentin, TUJ1, GFAP and MAP2. The level of expression of mRNAs for nestin, vimentin, CD133, and TUJ changed between both states. The levels of expression of mRNAs for GFAP and MAP2 did not vary between phenotypic states, but showed a peak in expression during the transition from one state to the other (Figure (Figure77).
We next sought to compare the genetic profiles of each phenotype using cDNA microarray analysis to understand differential gene expression between both phenotypes. Figure Figure88 shows the top 100 genes that were differentially expressed between the adherent and sphere phenotypes across HF2303, MGG8, IN859, and IN2045 patient derived glioblastoma cell cultures. Note that the location of cGMP-dependent protein kinase 1 (PRKG1) is highlighted. This gene was overexpressed in the four cells studied, and was further examined as shown below.
We explored the genomic landscape of HF2303 and IN2045 glioma stem cells to discover further potential therapeutic targets. From our microarray expression database we extracted the 100 most highly expressed genes and the 100 most highly inhibited genes when gene expression from the sphere condition was compared to the adherent condition. This set of genes, which thus contains the genes most upregulated and those most downregulated in cells grown as tumorspheres, did contain PRKG1 as expected. This study was conducted for the human glioma cell lines HF2303 and IN2045. The resulting networks are illustrated in Figures Figures9,9, ,1010 and Table Table1.1. The HF2303 network displayed a protein-protein interaction (PPI) enrichment p-value of 0.00162. The IN2045 network displayed a PPI enrichment p-value of 1.62×10−11. In both networks PRKG1 was strongly associated with CFTR. We identified the gene ontologies which contained both PRKG1 and CFTR, and used the genes enriched within these three ontologies to construct a combined network (Mix in Figure Figure9).9). Data on the effetcs of PRKG1 are shown in Figures Figures1111 and and12.12. The combined network's PPI enrichment p-value was 0. This network includes mRNAs from genes already known to be of importance in glioma biology, e.g., PDGFRA, met, and TGF-β2. The combined network also indicates novel genes that ought to be explored as future potential targets for the treatment of glioblastoma multiforme. We highlight the cystic fibrosis transmembrane regulator (CFTR), the phospholipase PLCB1, and the transcription factor TBX2, amongst others to be found in Figure Figure9.9. Very little information is currently known about the activities of these gene products in glioma biology. Figures 10a, 10b and Table Table11 show the gene ontology networks that correspond to the gene Figure Figure9.9. The gene ontologies and the gene ontology networks illustrate the close correlation of genes involved in brain and neuronal development and genes activating transcription factor function, all of which are enriched within the sphere phenotype of two human glioma stem cell lines.
PRKG1 is differentially overexpressed in the sphere phenotype in four human glioma stem cell lines, i.e., HF2303, MGG8, IN859 and IN2045. In all cell lines, PRKG1 was within the 100 genes with the highest differential expression between the sphere and the adherent phenotype. If we assume that PRKG1 overexpression is a result of reduced activation, we reasoned that activation of PRKG1 could induce its pro-apoptotic effects. PRKG1 can be permanently activated using the cGMP analogue 8-Br-PET-cGMP. We tested whether activation of PRKG1 would result in decreased cell viability in our model. For the adherent phenotype, activation of PRKG1 reduced cell viability compared to control in both patient derived glioblastoma cell cultures (Figure 11A-11B). The effect was greater for the sphere phenotype, and activation of PRKG1 resulted in reduced cell viability in both patient derived glioblastoma cell cultures (Figure 11C-11D). Maximal effect was observed in HF2303 spheres at 72 hours with a dose of 100 μM 8-Br-PET-cGMP which resulted in an over 50% reduction in cell viability compared to control.
Mitomycin was used to arrest the cell cycle and cell proliferation to test whether the effect of PRKG1 was cell cycle dependent or independent. For both HF2303 and IN2045, the effect of doxorubicin (an inhibitor of topoisomerase II, and cell-cycle dependent cytotoxin) was abrogated by mitomycin. Conversely, mitomycin had no effect on treatment with 8-Br-PET-cGMP suggesting that downstream reduction in cell viability seen with PRKG1 activation occurs in a cell cycle independent manner (Figure 12A, 12C).
As we have shown, the sphere phenotype is normally more resistant to traditional agents such as doxorubicin (Figure 12D), whereas the adherent phenotype shows a much higher sensitivity to doxorubicin (Figure 12B). The converse is true for PRKG1 activation, where the sphere phenotype is more sensitive (Figure 12B, 12D). When PRKG1 activation is added to doxorubicin, in both the adherent and sphere phenotype there is a reduction in cell viability compared to either PRKG1 activation or doxorubicin alone. This suggests that doxorubicin and 8-Br-PET-cGMP may be inhibiting growth of different cell populations. (Figure 12B, 12D)
The growth of human gliomas is thought to follow the cancer stem cell model. Variable experimental definitions of glioma stem cells in vitro has led to discrepancies in the literature concerning the behavior and characterization of glioma stem cells. This is particularly evident in the varying methods used to study and culture glioma stem cells. The most commonly employed methods for culture utilize serum-containing media to generate adherent monolayers, serum-free media containing EGF and bFGF to generate neurosphere-like tumorspheres, and putative increased expression of CD133 in stem cells. Formation of neuro/tumorspheres in vitro coupled to in vivo tumor growth are the most reliable methods to identify glioma stem cells. Cells growing as spheres are thought to be able to grow as adherent cells if the medium is changed; adherent cells however, are not supposed to be able to grow as spheres.
Unexpectedly our experiments showed that glioma stem cells can grow reversibly (i.e., interchange from one phenotype to the other and vice-versa), but that each phenotype differs in its behavioral characteristics vis-à-vis proliferation, invasion, and chemoresistance.
Our results show that all patient derived glioblastoma cell cultures utilized in this study are capable of growth as an adherent monolayer and as tumorspheres. These growth patterns were reversible by transferring cells from adherent conditions to sphere conditions or vice versa. For all glioma stem cells, cells cultured under adherent conditions displayed a higher rate of proliferation, higher rate of invasion, and lower chemoresistance compared to the same cells cultured under sphere conditions. The exception was the chemosensitivity assays performed with temozolomide which showed little cytotoxicity in either the adherent or sphere phenotypes. These observations imply that these assays are dependent on the culture method employed. Thus, these results serve to point out that interpretation of in vitro studies involving glioma stem cells is highly dependent on the method of study being employed.
Reversibility of sphere and adherent phenotypes led us to predict that if cells were placed in similar microenvironments in vitro or in vivo their behavior may equally become alike. In vivo we were able to test this hypothesis by implanting equal numbers of cells into the striatum of mice. Tumor growth rate was similar for both groups. This suggests that the sphere-state and adherent-state, at least for the cells tested, are reversible in vivo. It is thus likely that tumors are composed of cells in various phenotypic states, two of which are the in vitro sphere and adherent phenotypes which are reversible.
Based on these results, we propose a simple experimental definition of glioma propagating/initiating cells (GPCs). We propose that all cells referred to as GPCs should grow reversibly as an adherent monolayer and as tumor spheres in vitro and should form tumors following in vivo implantation. Utilizing this simple experimental definition may facilitate inter-laboratory experimental replication. Also, work from other investigators can easily prove, disprove, expand, or amend this definition. We also suggest that assays be interpreted in the light of the phenotype used. Utilizing this definition we were able to identify a new therapeutic target to be used with standard treatment of care such as chemotherapy and radiation.
Genetic expression profiles were used to evaluate the genes/signaling pathways underlying the state-specific behaviors, and to compute comparative gene expression maps to identify actionable targets for specific killing of cells in the chemoresistant sphere-state. cDNA microarrays were used to determine the genetic expression profile of the sphere and adherent phenotypes and to generate a list of genes over- and underexpressed in each phenotype. Expression of various mRNAs (i.e., CD133 and others) was also reversibly expressed between the adherent and sphere phenotype.
One gene that was selectively overexpressed in the sphere phenotype was cGMP dependent protein kinase 1 (PRKG1). Review of the literature revealed previous reports of involvement of PRKG1 in apoptosis of cancer cells via hyperactivation of death-associated protein kinase 2 [20–22]. We hypothesized that overexpression of PRKG1 in the sphere phenotype could be a compensatory mechanism for low endogenous activation. We thus assumed that if increased amounts of PRKG1 could be exogenously activated this might induce apoptosis in the normally resistant PRKG1 overexpressing spheres. Our experimental results support our assumptions.
Analysis of the networks formed by HF2303 or IN2045 demonstrated a close association of PRKG1 with CFTR, two proteins not yet linked to glioblastoma biology. Ontologies containing both genes were used to create a combined network. This combined network linked both PRKG1 and CFTR to genes already known to be of importance in glioma biology such as PDGFRA, met, and TGFβ2, amongst others. This provides support for the soundness and biological significance of the protein-protein interaction networks (PPI) and gene ontology networks uncovered. Further, this implies that mining of these networks will identify novel potential therapeutic molecules. Examination of the PPI interaction networks suggests that the ion channel CFTR, the phospholipase PLCB1, and the transcription factor TBX2 ought to be considered novel potential proteins to be inhibited for the treatment of glioblastoma. The ontology networks providing the functional integration of network genes affords further support for the validity of this analysis by highlighting ontologies related to neuronal development, and activating transcription factor activity.
8-Br-PET-cGMP is a highly specific activator of both the A and B isoforms of cGMP-dependent protein kinase I (with very poor activation of type II). Treatment of cells with 8-Br-PET-cGMP, i.e., activation of PRKG1, resulted in a significant and dose-dependent reduction of cell viability in the sphere phenotype that overexpresses PRKG1. This suggests that the observed reduction in cell viability is due to activation of apoptosis.
Most traditional chemotherapeutic agents are cell cycle dependent as they damage DNA or disrupt components of the cell division machinery resulting in cell death. Doxorubicin, for example, stabilizes the topoisomerase II complex preventing cell replication. When pretreated with mitomycin to shut down the cell cycle, the killing effect of doxorubicin was abrogated. However, when cells were pretreated with mitomycin the cytocidal effect of activation of PRKG1 was not abrogated, especially within spheres. This suggests that, in comparison to most traditional chemotherapeutic agents, the effect of activation of PRKG1 ocurs in a cell cycle independent fashion. In addition, the combination of PRKG1 activation and doxorubicin led to a larger reduction in cell viability than either alone. Together with the observation that doxorubicin works in a cell cycle dependent fashion, while activation of PRKG1 is likely to work in a cell cycle independent fashion, suggests that activation of PRKG1 may be acting on cells in a different phenotype and state, i.e., slowly dividing sphere glioma cells, than doxorubicin.
PRKG1 appears to be a novel therapeutic phenotype-specific drug for the treatment of glioblastoma. It warrants future in vivo testing to determine whether activation of PRKG1 reduce tumor burden and extends survival either alone or in combination with standard therapy. Its ability to kill the normally chemoresistant sphere phenotype while traditional chemotherapy agents are more efficacious for the adherent phenotype combined with observation that activation of PRKG1 may be inhibiting a different subpopulation of cells than traditional chemotherapeutic agents make it a particularly attractive addition for testing in combination with traditional therapies. Moreover, we have potentially identified a new method for generating candidate therapeutic agents and have generated a list of up- and downregulated genes in each phenotype that can be further tested as therapeutic candidates.
In summary, we illustrate the reversibility of human glioma stem cells in vitro between a sphere phenotype and an adherent phenotype, and that this morphological phenotype correlates with cellular proliferation rate, invasion capability, and response to chemotherapy. However, in a comparable microenvironment (e.g., normal brain) both phenotypes grew tumors at comparable rates; this result is predicted by the phenotype reversibility detected. Finally, the differential genomic landscape between phenotypes was utilized to identify a novel potential drug that inhibits selectively the sphere phenotype for which few pharmacological agents exist. PPI and gene ontology networks examined suggest the existence of further targets to be explored for the treatment of glioblastoma, such as PRKG1, CFTR, PLCB1, and TBX2.
The HF2303 human gliosarcoma patient derived glioblastoma cell culture was obtained from Dr. Tom Mikkelsen at Henry Ford Hospital (Detroit, MI). The MGG8 human glioma patient derived glioblastoma cell culture was obtained from Samuel Rabkin, Harvard University. The IN859 and IN2045 patient derived glioblastoma cell cultures were obtained from Dr. John Darling, University College London. Experiments described in detail below were all performed in triplicate.
Six to eight week-old male/female Rag1tm1Mom/J were purchased from Jackson Laboratory (Bar Harbor, ME) and subsequently bred in-house to maintain a colony. All animal procedures were approved by the University Committee on Use and Care of Animals (UCUCA) at the University of Michigan.
CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay reagents were purchased from Promega (Madison, WI). For each patient derived glioblastoma cell culture, 7500 cells were plated in 100 μL of media in each well of a 96-well plate. Cells were plated in four wells per experimental group. Additionally, blank wells were plated consisting of medium alone. At 24, 48, and 72 hours MTS solution was added to each well and incubated for 3 hours at 37°C in a 5% CO2 humidified atmosphere. After colorimetric development, the plate was read at 490 nm on a PerkinElmer EnSpire 2300 Multimode Plate Reader (Waltham, MA). The average blank value was then subtracted from each experimental well.
BD BioCoatTM BD MatrigelTM Invasion Chamber inserts with 8 μm pores and BD BioCoatTM Control inserts with 8 μm pores were purchased from BD (Franklin Lakes, NJ). Inserts were rehydrated in medium for 2 hours at 37°C in a 5% CO2 humidified atmosphere. The medium was then removed. In the bottom of a 24 well plate 750 μL of DMEM supplemented with 10% FBS was placed as the chemoattractant. The inserts were then placed into each well of the 24 well plate. On the interior of the inserts, 25,000 cells in 500 μL of serum-free DMEM were plated. Plates were then incubated for 24 hours at 37°C in a 5% CO2 humidified atmosphere. After incubation, the non-invading cells were removed from the upper surface of the membrane by scrubbing the membrane with a cotton-tipped swab. The membranes were then cut out of the inserts and fixed and stained with Differential Quik Stain (Polysciences, Inc.; Warrington, PA). The number of invading/migrating cells was then quantified by counting the number of cells per 20X field. Five random fields were counted per well and three wells were used for each experimental group. The experiment was repeated twice. Migrating cells were those cells present on the under surface of the control inserts. Invading cells were those cells present on the under surface of the Matrigel inserts. An invasion index was calculated by taking the mean number of invading cells, dividing by the mean number of migrating cells, and multiplying by 100.
Temozolomide, paclitaxel, cisplatin, and doxorubicin were purchased from Sigma-Aldrich (St. Louis, MO). A total of 5000 cells in 100 μL were plated into each well of a 96-well plate. Cells were incubated for 24 hours at 37°C in a 5% CO2 humidified atmosphere. After 24 hours, the chemotherapeutic agent was added to each well at the specified concentration. The chemotherapeutic agent was dissolved in media at 11X final concentration and then 10 μL was added to each well. The plates were then incubated for an additional 72 hours. At that time, MTS assays were performed as previously described. Each experimental group was performed in triplicate and the experiment was performed three times. Percent cell viability was calculated by dividing the mean absorbance of the experimental group by the mean absorbance of the control group (no chemotherapeutic agent added) and multiplying by 100.
Six to eight week old female Rag1tm1Mom/J mice were utilized for tumor implantation. Mice were anesthetized with intraperitoneal injection of 0.5 mg/kg dexmedetomidine and 75 mg/kg ketamine. Mice were then placed into a stereotactic frame a single midline cranial incision was made. Coordinates based off of bregma were used for placement of a burr hole at +0.5 mm anterior-posterior and +2.5 mm medial-lateral (right). A burr hole was placed at this location exposing the underlying dura which was opened and reflected using a 27 gauge needle. A total of 30,000 cells in 1 μL of DMEM were then injected at a depth of 3.0 mm from the brain surface using a 5 μL Hamilton syringe equipped with a 33-gauge needle. Cells were allowed to settle for 6 minutes prior to slow withdrawal of the needle. The skin was then sutured and reversal agent consisting of atipamezole 1 mg/kg intraperitoneal was given. Post-operative pain relief was achieved via buprenorphine 0.1 mg/kg given subcutaneously. At specified time points or when moribund mice underwent transcardial perfusion oxygenated, heparinized (100 units/L) Tyrode's solution followed by 4% paraformaldehyde (PFA). Brains were harvested and then post-fixed in 4% PFA for 2-3 days. Brains were sectioned using a vibratome yielding 50 μm sections for further analysis.
For both DAB peroxidase immunolabeling and immunofluorescence, brain sections were transferred into 12 well plates containing TBS-0.5% Triton X (Tx) for 1 hour while shaking. Antigen retrieval was performed using 10 mM sodium citrate solution 60-90°C for 20 minutes followed by room temperature sodium citrate solution for 20 minutes. For DAB peroxidase staining, sections were then incubated with 5% hydrogen peroxide for 30 minutes to quench endogenous peroxidase activity. After washing with TBS-Tx, non-specific antibody binding was blocked using TBS-Tx with 10% horse serum for 2 hours at room temperature while shaking. Next sections were incubated with primary antibody diluted in TBS-Tx/1% horse serum/0.1% sodium azide at 4°C for 48 hours while continuously shaking. After washing with TBS-Tx, sections were incubated in appropriate biotinylated secondary antibody diluted in TBS-Tx/1% horse serum for 24 hours at 4°C while continuously shaking. Sections were then washed in TBS-Tx and incubated in Vectastain Elite ABC system (Vector Laboratories; Burlingame, CA) AB solution for 6 hours at room temperature. After washing with PBS followed by 0.1 M sodium acetate (pH 6.0), sections were stained in DAB staining solution. Reactions were stopped using 10% azide and then washed sequentially in sodium acetate followed by PBS. Sections were then dehydrated and mounted using Histomount. Anti-human nucleus (1:500, Millipore MAB1281) was used and then developed using secondary biotinylated rabbit anti-mouse (1:1000, Dako E0464).
All patient derived glioblastoma cell cultures were maintained in vitro in 95% air/5% CO2 at 37°C under humidified conditions. Culture medium for adherent growth of HF2303, MGG8, IN859, IN2045 consisted of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 0.3 mg/mL L-glutamine, 50 units/mL penicillin, 50 μg/mL streptomycin, and non-essential amino acids. This medium is referred to as “adherent medium” throughout the manuscript. Cells were passaged every 3-4 days. Culture medium for HF2303, IN859, and IN2045 tumorspheres consisted of DMEM/F12 supplemented with N2 supplement, 50 units/mL penicillin, 50 μg/mL streptomycin, antibiotic/antimycotic (0.5X final concentration), 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF). Culture medium for MGG8 tumorspheres consisted of Neurobasal Medium supplemented with B27 supplement, 0.45 mg/mL L-glutamine, N2 supplement (0.5X final concentration), 50 units/mL penicillin, 50 μg/mL streptomycin, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF). Medium for tumorsphere growth is referred to as “sphere medium” throughout the manuscript. Half culture medium was replaced every 3 days with new EGF and bFGF supplementation. Cells were dissociated using HyClone HyQtase every 6-7 days and split 1:4.
For real-time quantitative reverse transcription-PCR (RT-PCR) analysis, total RNA was isolated from cells using a NucleoSpin RNA II kit (Macherey-Nagel; Bethlehem, PA). Five micrograms of total RNA were used for reverse transcription. First-strand cDNA was generated using oligo(dT)18 (Fermentas) and Superscript II RT (Invitrogen). Two microliters of the resulting cDNA (1:10 dilution) were used in the real-time reactions with gene-specific primers. The following gene-specific primers were used: Nestin 5′ - 3′ and 5′ - 3′, Vimentin, CD133, TUJ1, GFAP, MAP2, and GAPDH. Quantitative RT-PCR reactions were carried out with FastStart SYBR Green Master mix (Roche) and MACHINE. Fluorescence intensity was measured at the end of each elongation step as a means to evaluate the amount of formed PCR product. GAPDH was used as a reference to normalize the samples.
Total RNA was isolated from HF2303, MGG8, IN859, and IN2045 grown under adherent and sphere conditions using a NucleoSpin® RNA II kit (Macherey-Nagel; Bethlehem, PA). Genetic expression of the cells grown under adherent conditions was compared to expression of cells grown under sphere conditions using an Affymetrix GeneChip® Human Gene 2.0 ST Array. The quantity of a given transcript was compared using a signal log algorithm in two arrays, a baseline array versus a control array. The baseline array in this case was the cells grown under adherent conditions and the experimental array was the cells grown under sphere conditions. The change in the transcript level was then expressed as a log2 ratio where a value of 1.0 indicates a 2-fold increase expression of a given gene under adherent conditions and a value of -1.0 represents a 2-fold increase expression of a given gene under sphere conditions. A composite analysis was then performed treating the adherent conditions for all four patient derived glioblastoma cell cultures as the baseline group and the sphere conditions for all four patient derived glioblastoma cell cultures as the experimental group.
Adherent cells were plated in 96 well plates at a density of 1000 cells in 100 μL of medium. Dissociated spheres were plated in 96 well plates at a density of 10,000 cells in 100 μL of medium. Cells were then incubated for 24 hours at 37°C in a 5% CO2 humidified atmosphere. At that time, cells were treated daily with 8-Br-PET-cGMP (Axxora P007; Farmingdale, NY) at the specified concentrations in order to activate cGMP-dependent protein kinase 1. After 48 hours from the initial treatment, MTS assays were performed as previously described. Percent cell viability was calculated by dividing the mean absorbance of the experimental group by the mean absorbance of the control group (no treatment) and multiplying by 100. Cells were plated in four wells per experimental group and the experiment was repeated three times.
Non-toxic doses of mitomycin that led to cessation of cell proliferation were found for both HF2303 and IN2045 cells. Cells were plated at a density of 100,000 cells/mL in 96 well plates. Cells were allowed 24 hours to equilibrate. At that point, an MTS assay was performed to get a baseline absorbance. To the experimental plate, varying concentrations of mitomycin were added. Cells were allowed to incubate for 48 hours and then an MTS assay was performed. For both HF2303 and IN2045 the non-toxic dose that resulted in no change in absorbance over 48 hours was 10ug/mL (data not shown)
Next, for both HF2303 and IN2045 adherent and sphere a total of 5000 cells in 100ul were plated into each well of a 96 well plate. Cells were incubated for 24 hours at 37*C in a 5% CO2 humidified atmosphere. After 24 hours, mitomycin was added to each well. For those wells receiving doxorubicin or 8-Br-PET-cGMP, cells were preincubated for 6 hours with mitomycin. The doxorubicin concentration used was 0.1ug/mL while the 8-Br-PET-cGMP concentration was 100uM. Cells were then allowed to incubate for 48 hours and an MTS assay was performed.
The set of 200 genes most upregulated (100) or downregulated (100) in the sphere phenotype v. adherent phenotype in human glioma stem cell lines HF2303 or IN2045 was uploaded into STRING (http://string-db.org/) and networks were evaluated. The minimum required interaction score was set to high confidence (0.700), and the active interaction sources used were: textmining, experiments, databases, neighbourhood, gene fusion, co-occurrence and co-expression. For the view used to prepare the data for Figure Figure99 disconnected nodes in the network were hidden from view. Figures of the networks, and quantitative network characteristics and gene ontologies were downloaded. The networks are shown in Figure Figure9,9, the gene ontologies hierarchy of the combined network are shown in Figures 10a and 10b, and the gene ontologies for the HF2303 and IN2045 networks are illustrated in Table Table1.1. Gene ontologies (GO) were analyzed and visualized using http://geneontology.org and its analysis and visualization tool Amigo2 (http://amigo.geneontology.org/visualize?mode=client_amigo).
Statistical analysis was performed using commercially available software (SPSS version 21, IBM Corp.). For univariate analysis of continuous variables with a normal distribution we planned to utilize 2-sample t-tests, and for continuous variables not meeting the normality assumption we planned to utilize the Mann-Whitney U-test. Data were screened for normality using normality plots and the Shapiro-Wilks test. In all instances, the data were non-parametric, so only the Mann-Whitney U-test was utilized. A p-value < 0.05 was considered statistically significant.
We gratefully acknowledge Mr. Philip Jenkins and the Dept. of Neurosurgery at the University of Michigan Medical School for their support of our work. We are also grateful to Dr. Karin Muraszko for her academic leadership, S Napolitan, and M. Dahlgren for superb administrative support and M Dzaman for outstanding technical support. Dr. Irina Kalatskaya comments on bioinformatics and the manuscript are greatfully acknowledge. We acknowledge the use of the open software STRING, produced by the Swiss Institute of Bioinformatics, EMBL Heidelberg, University of Zurich, NNF Center For Protein Research, Technical University Dresden, and former contributors as listed in the STRING website http://string-db.org/).
CONFLICTS OF INTEREST
We have no conflicts of interest to declare
This work was supported by National Institutes of Health/National Institute of Neurological Disorders & Stroke (NIH/NINDS) Grants R01-NS094804, R01-NS074387, R21-NS091555, and R37-NS094804 to M.G.C.; NIH/NINDS Grants R01-NS076991, R01-NS082311, R21-NS084275, and R01-096756 to P.R.L.; Leah's Happy Hearts, University of Michigan Comprehensive Cancer Center, Chad Tough Foundation, and The Phase One Foundation to both M.G.C. and P.R.L.; the Department of Neurosurgery, University of Michigan School of Medicine; the Michigan Institute for Clinical and Health Research, NIH 2UL1-TR000433; University of Michigan Cancer Biology Training Grant, NIH/NCI (National Cancer Institute) T32-CA009676; University of Michigan Training in Clinical and Basic Neuroscience, NIH/NINDS T32-NS007222; and the University of Michigan Medical Scientist Training Program, NIH/NIGMS (National Institute of General Medicine Sciences) T32-GM007863.