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Calcium is the most abundant mineral in human body and essential for the formation and maintenance of bones and teeth as well as diverse cellular functions. Calcium carbonate (CaCO3) is widely used as a dietary supplement; however, oral absorption efficiency of CaCO3 is extremely low, which may be overcome by applying nano-sized materials. In this study, we evaluated the efficacy of food grade nano CaCO3 in comparison with that of bulk- or reagent grade nano CaCO3 in terms of cytotoxicity, cellular uptake, intestinal transport, and oral absorption. Cytotoxicity results demonstrated that nano-sized CaCO3 particles were slightly more toxic than bulk materials in terms of oxidative stress and membrane damage. Cellular uptake behaviors of CaCO3 nanoparticles were different from bulk CaCO3 or Ca2+ ions in human intestinal epithelial cells, showing efficient cellular internalization and elevated intracellular Ca2+ levels. Meanwhile, CaCO3 nanoparticles were efficiently transported by microfold (M) cells in vitro model of human intestinal follicle-associated epithelium, in a similar manner as Ca2+ ions did. Biokinetic study revealed that the biological fate of CaCO3 particles was different from Ca2+ ions; however, in vivo, its oral absorption was not significantly affected by particle size. These findings provide crucial information to understand and predict potential toxicity and oral absorption efficiency of food grade nanoparticles.
Calcium is essential for the formation and maintenance of bones and teeth, cellular physiology, immune response, hormone secretion, activation of enzymes, and blood-clotting system [1,2]. Calcium carbonate (CaCO3) is the most prevalent form of a calcium supplement due to its abundance from nature, such as oyster and sea shells, as well as the most cost-effective . However, oral bioavailability of CaCO3 is extremely low, since calcium is well absorbed into the body under acidic conditions and an alkaline CaCO3 requires stomach acid for better absorption [3,4,5]. Moreover, solubility of CaCO3 is known to be generally low compared to other inorganic nanoparticles . The low efficacy of CaCO3 may be overcome by applying nano-sized materials, which can induce cellular uptake by endocytosis [7,8], different uptake pathways from that for Ca2+ ions . Much research has demonstrated that nanoparticles can be internalized into cells by energy-dependent endocytosis, consequently contributing to enhanced uptake efficacy [10,11,12].
In order to obtain food grade CaCO3 particles, a mechanical grinding process from oyster or sea shells, namely the top-down approach, is generally applied. However, along with extensive application of nanotechnology to diverse fields, increasing concern about potential adverse effects of nanomaterials on human body has been raised, needing verification on their toxicity [13,14]. According to The United States Toxic Substances Control Act inventory, a lethal dose of 50% (LD50) of CaCO3 is 6450 mg/kg), being classified in the group with the least toxicity. Indeed, Jeong et al. demonstrated that nano CaCO3 did not cause toxicity up to 2000 mg/kg after a 14-day oral repeated dose administration to rats, supporting its low toxicity . Moreover, biological fates of nanoparticles are necessary to be determined, especially for nanomaterials that can be partially dissolved into ions under physiological condition, in order to understand and predict their potential toxicity and toxic mechanisms [16,17,18]. On the other hand, conflicting results have been reported on increased bioavailability of CaCO3 nanoparticles compared to that of bulk materials [19,20,21], and, therefore, further elucidation on enhanced efficacy of nanoparticles through the gastrointestinal tract is necessary [22,23].
In this study, we investigated the effects of particle size (bulk versus nano) of food grade CaCO3 particles (food bulk CaCO3 and food nano CaCO3), both produced by grinding sea shells, on cytotoxicity, cellular uptake behaviors, and intestinal transport. Furthermore, oral bioavailability of CaCO3 particles with respect to particle size was evaluated after a single-dose oral administration to rats. A comparative study with reagent grade CaCO3 particles (SS CaCO3), which was produced by the bottom-up approach of high gravity reactive precipitation, or Ca2+ ions was also performed to compare biological responses of CaCO3 particles prepared by different methods and to answer the question as to whether the effects of CaCO3 particles result from particulate forms or ionic forms in the biological system.
Figure 1 demonstrates scanning electron microscopy (SEM) images of three different CaCO3 particles. Particle size of food bulk CaCO3 particles was heterogenous, showing an irregular shape, while food nano CaCO3 and reagent grade SS CaCO3 had relatively homogenous size distributions. An average primary particle size of food bulk CaCO3, food nano CaCO3, and SS CaCO3 were determined to be ~2 μm, ~100 nm, and ~110 nm, respectively. Specific surface areas measured by nitrogen adsorption-desorption isotherm were 1, 16 and 21 m2/g for food bulk CaCO3, food nano CaCO3, and SS CaCO3, respectively, indicating that smaller particles tend to have larger specific surface area. Slightly larger surface area of SS CaCO3 than food nano CaCO3 in spite of larger primary particle size of the former rather than the latter, was thought to be originated from the uniform particle size distribution of SS CaCO3. On the other hand, SEM images showed that food bulk CaCO3 and food nano CaCO3 had different surface smootheness compared to SS CaCO3, probably a result of different synthetic methods.
Indeed, atomic force microsope (AFM) demonstrated surface roughness of each CaCO3 (Figure 1) and showed remarkably smooth surfaces of SS CaCO3 compared with others. Calculated surface roughness parameters, Ra were 36.4, 7.9 and 1.8 nm for food bulk CaCO3, food nano CaCO3, and SS CaCO3, respectively. It has been reported that surface roughness of nanoparticles affected their biological behaviors including cytotoxicity , as their surface roughness minimize repulsive force between nanoparticles and plasma membrane, possibly influencing membrane damage or cellular uptake . Thus, different surface roughness as well as particle size could result in different biological responses. It is worth noting that food grade CaCO3 particles were produced by the top-down of grinding sea shells and SS CaCO3 was obtained by the bottom-up of high gravity reactive precipitation. Zeta potentials of food bulk CaCO3, food nano CaCO3, and SS CaCO3 were −3.7 ± 1.9, 15.7 ± 0.5, and 11.8 ± 0.8 mV, respectively, indicating that surface charge of CaCO3 nanoparticles was different from bulk CaCO3. On the other hand, solubility of all CaCO3 particles was less than 0.01 and 0.1% (w/v) in physiological fluid at pH 7.0 and simulated gastric fluid at pH 1.5, respectively, suggesting that extremely low amount of CaCO3 particles can be partially dissolved into ions even under gastric conditions, regardless of particle size.
In order to investigate hydrodynamic radii of CaCO3 particles after in vivo administration, we measured dynamic light scattering (DLS) pattern of CaCO3 particles in albumin solution (Figure 2). Although larger hydrodynamic diameters of all particles compared with their primary particle size (Figure 1) were observed, it was clearly noted that average value and distribution was different with respect to particle size and manufacturing method. Food bulk CaCO3 showed large diameter up to 6000 nm with wide full-width at half-maximum (FWHM), whereas those of food nano and SS CaCO3 were smaller and more narrow than bulk particles. SS CaCO3 particles showed slightly narrow FWHM value compared to food nano CaCO3, possibly due to the homogeneous particle size distribution as observed in SEM (Figure 1).
To evaluate the effect of CaCO3 particles on cytotoxicity with respect to particle size, cell proliferation was measured with water-soluble tetrazolium salt (WST-1) assay in human intestinal INT-407 cells. In all experiments, an equivalent amount of CaCl2 as Ca2+ ions was used to allow cytotoxicity and uptake behaviors of CaCO3 particles and Ca2+ ions to be compared. As shown in Figure 3A, cell proliferation was not affected by all three different CaCO3 particles when the cells were exposed to 250 μg/mL particles for 1–24 h. Furthermore, no effect of particle size of CaCO3 on cell proliferation was found up to the highest concentration tested, 1000 μg/mL (Figure 3B), after 24 h of incubation, indicating their low cytotoxicity. Further cell exposure to nanoparticles for 72 h did not cause inhibition of cell proliferation (data not shown). Ca2+ ions did not exhibit cytotoxicity as well under the same experimental condition.
Generation of intracellular reactive oxygen species (ROS) was monitored using a cell permeant fluorescent probe. Figure 4A demonstrates that ROS significantly increased in INT-407 cells exposed to nano-sized materials, both food nano CaCO3 and SS CaCO3, at above 125 μg/mL. In particular, slightly high ROS generation was induced by food nano CaCO3 compared to SS CaCO3 at high concentration of 500–1000 μg/mL. Interestingly, food bulk CaCO3 nor Ca2+ ions did not generate ROS, suggesting that nanoparticles were more cytotoxic than bulk materials or Ca2+ ions.
When released levels of intracellular lactate dehydrogenase (LDH) into the extracellular medium was evaluated (Figure 4B), the highest LDH leakage was induced by food nano CaCO3, followed by food bulk CaCO3 ≈ SS CaCO3 > CaCl2. Taken together, food nano CaCO3 exhibited the highest cytotoxicity in terms of ROS generation and membrane damage, although it did not inhibit cell proliferation (Figure 3). It seems that CaCO3 nanoparticles can damage the cell membrane and consequently induce ROS, but these cytotoxic effects are not severe to affect cell prolliferation. It was reported that layered double hydroxide nanoparticles did not block cell proliferation up to 500 μg/mL, but caused ROS generation and LDH release [26,27]. Slightly high cytotoxicity of food nano CaCO3 is likely to be associated with surface roughenss resulting from the grinding process of sea shells as shown in AFM images and height profile in Figure 1B. Rough surface of food nano CaCO3 could maximize attractive interaction between particles and cellular membranes , subsequently inducing more oxidative stress as well as membrane damage than smooth surfaced SS CaCO3. Meanwhile, it should be noted that an equivalent amount of Ca2+ ions caused little cytotoxicity, and all CaCO3 particles had extremely low solubility. Therefore, cytotoxicity of CaCO3 particles seems to be related to their particulate fate under cell culture conditions.
Cellular uptake of CaCO3 particles was evaluated by measuring total calcium levels in particle-treated INT-407 cells using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), in order to investigate the effects of particle size on cellular internalization. Figure 5A shows that cellular internalization of CaCO3 particles remarkably increased as particle size decreased under nomal condition at 37 °C after 2 h of incubation, as evidenced by significantly high uptake of both food nano CaCO3 and SS CaCO3 compared to that of food bulk CaCO3. The cellular uptake behaviors showed correlation with specific surface area of CaCO3 particles, in other words, particles with larger surface area had higher cellular uptake. Furthermore, CaCO3 particles were more massively internalized into cells than Ca2+ ions, indicating different uptake pathways between particles and Ca2+ ions.
When the role of energy-dependent internalization in paricle uptake was examined by incubating the cells at 4 °C (Figure 5A), cellular uptake of all CaCO3 particles significantly decreased in comparison with that obtained at 37 °C, regardless of particle size, showing 41.65%, 45.96%, and 37.93% inhibitions for food bulk CaCO3, food nano CaCO3, and SS CaCO3, respectively. This result suggests that all CaCO3 particles can partially enter the cells by energy-dependent endocytosis. Uptake of Ca2+ ions was not affected by low temperature, probably attributed to their different internalization pathway from that for CaCO3 particles, which does not need energy for uptake.
On the other hand, when intracellular uptake of CaCO3 particles was monitored with the Ca2+ probe (Figure 5B), significantly elevated Ca2+ levels were found inside the cells treated with SS CaCO3 and food nano CaCO3. Since the Ca2+ probe detects only ionized Ca2+ ions from CaCO3 particles, thus elevated intracellular Ca2+ levels in the presence of nanoparticles suggest that SS CaCO3 and food nano CaCO3 can be more effectively taken up by cells and easily dissolved into Ca2+ ions inside the cells than food bulk CaCO3. Meanwhile, a significant difference in Ca2+ levels between CaCO3 nanoparticles and Ca2+ ions is in good agreement with cellular uptake measured by ICP-AES (Figure 4A), which can be explained by efficient cellular uptake behaviors of CaCO3 nanoparticles as compared with Ca2+ ions.
Further mechanistic study on the transport of three different CaCO3 particles across the intestinal epithelium was carried out using 3D cell culture system, in vitro model of human intestinal follicle-associated epithelium (FAE), based on co-culture of human intestinal epithelial Caco-2 cells and human Raji B lymphocytes [28,29]. The FAE is different from normal intestinal epithelium and contains specialized microfold (M) cells that are capable of transporting a wide range of materials, such as bacteria, viruses, macromolecules, and particles [30,31]. Thus, the role of M cells in in vivo particle absorption across the intestinal epithelium can be evaluated using the in vitro FAE model. Figure 6 shows that the transport of food nano CaCO3, SS CaCO3, and CaCl2 by M cells significantly increased, while elevated transport of food bulk CaCO3 was not found, suggesting that M cells are the transport mechanism for both CaCO3 nanoparticles and Ca2+ ions. In particular, the transport of SS CaCO3 was similar to that of CaCl2. Hence, it seems that reagent grade SS CaCO3 with a smooth surface and narrow size distribution compared to food nano CaCO3 (Figure 1) is more favorable to be transported by M cells.
On the other hand, this result also implies that bulk particles cannot be transcytosed by M cells, possibly leading to low in vivo oral absorption efficiency. It is worth noting that the same tendency was obtained for food bulk CaCO3 in Figure 5B and Figure 6, showing neither elevated intracellular Ca2+ levels nor increased intestinal transport, whereas, significant cellular uptake was measured by ICP-AES analysis (Figure 5A). It is probable that bulk materials are somewhat adsorbed on the cell plasma membrane, which may result in totally elevated false cellular uptake by ICP-AES, although 5 mM EDTA was treated to remove particles not taken up by the cells. Here, Figure 5B only measured intracellular Ca2+ levels, while Figure 6 represented total transported calcium amount into basolateral solution in FAE model, reflecting intestinal absorption by M cells. Little cellular uptake, but high intestinal transport of Ca2+ ions, as shown in Figure 5 and Figure 6, implies that extremely low levels Ca2+ ions are taken up by cells in ionic state, probably due to calcium homeostasis, but they can be efficiently transported through the intestinal epithelium.
In vivo oral absorption of CaCO3 particles was also evaluated following a single-dose oral administration to rats. Figure 7 demonstrates different plasma concentration-time curves of three CaCO3 particles; SS CaCO3 and food nano CaCO3 particles showed more rapid absorption, showing peak concentration at 1 h versus 2 h for food bulk CaCO3. Interestingly, slightly high peak concentration at 1 h was found for SS CaCO3 than food nano CaCO3 and retarded decrease in peak concentration was observed for food nano CaCO3 compared to SS CaCO3. This might be explained by the different hydrodynamic size distribution in spite of similar specific surface area values between two nanoparticles, as shown in Figure 2; SS CaCO3 with narrow size distribution might be absorbed faster than food nano CaCO3, while food nano CaCO3 having larger hydrodynamic size is absorbed more slowly. The delayed absorption profile was also found for food bulk CaCO3, showing Tmax value at 2 h (Figure 7). On the other hand, Ca2+ ions were determined to behave differently from CaCO3 particles, with the highest maximum concentration at 15 min.
When biokinetic parameters of CaCO3 particles were compared (Table 1), significantly increased Cmax and shortened T1/2 and MRT values were examined for SS CaCO3 compared to food nano CaCO3 and food bulk CaCO3. Nevertheless, total oral absorption was not affected by particle size or surface roughness, as shown in similar AUC values and about 5% oral absorption for all CaCO3 particles. It is strongly likely that nanoparticles can more rapidly enter the systemic circulation than bulk-sized materials; however, particle size of CaCO3 does not influence total oral absorption efficiency. On the other hand, remarkably high oral absorption of Ca2+ ions as compared with CaCO3 particles was found, indicating different biological fates between CaCO3 particles and Ca2+ ions.
The bulk- and nano-sized food grade CaCO3 materials (food bulk CaCO3, food nano CaCO3) produced by grinding sea shells were purchased from Apexel Co., Ltd. (Pohang, Korea). Reagent grade CaCO3 (SS CaCO3), which was produced by high gravity reactive precipitation , was puarchased from Skyspring Nanomaterials Inc. (Houston, TX, USA). Particle size and morphology were examined by SEM (Hitachi S-4300, Tokyo, Japan). Surface roughness of each sample (0.1 g/mL), dispersed in ethanol, was measured with AFM (NX10, Park Systems, Suwon, Korea) and a drop of suspension was located on a flat silicon wafer. The surface charge (zeta potential) and size distribution of the particles were determined using a zeta potentiometer (Zetasizer Nano ZS system, Malvern Instruments, Worcestershire, UK). Specific surface areas were determined using N2 adsorption-desorption isotherms using a micromeritics ASAP 2020 (Accelerated Surface Area and Porosimetry System, Micromeritics Instrument Corporation, USA). Surface area values of CaCO3 were calculated with Brunauer-Emmett-Teller (BET) method. Adsorption isotherms below relative pressure 0.14 (for bulk CaCO3) and 0.25 (for food nano and SS CaCO3) were utilized for BET plot. In order to evaluate hydrodynamic size distribution of CaCO3 in physiological condition, each sample (1000 μg/mL) was dispersed in bovine serum albumin solution (200 μg/mL). Hydrodynamic size measurement was performed with DLS apparatus ELS-Z100 (Otsuka Electronics Co., Ltd., Osaka, Japan) three times with a refractive index of water 1.330.
Solubility was measured by dispersing 5 mg/mL particles in phosphate buffered saline (PBS, pH 7.0) or simulated gastric fluid (0.034 M sodium chloride, 3.2 g pepsin, pH 1.5). After incubation for 6 h at 37 °C, supernatants were collected by ultracentrifugation (15,000× g at 15 min). Then, calcium concentrations in the supernatants were determined by ICP-AES (JY2000 Ultrace; HORIBA Jobin Yvon, Stow, MA, USA).
Human intestinal epithelial (INT-407) cells were provided by Dr. Tae-Sung Kim at Korea University (South Korea) and cultured in MEM (Welgene Inc., Daegu, Korea) under a humidified atmosphere (5% CO2/95% air) at 37 °C. The medium was supplemented with 10% heat inactivated fetal bovine serum (Welgene Inc., Daegu, Korea), 100 units/mL penicillin, and 100 μg/mL streptomycin.
Effect of particles on cell proliferation was measured using the WST-1 assay (Roche, Basel, Switzerland). Briefly, cells (5 × 103/100 μL) were exposed to 1–1000 μg/mL particles for 24 h or to 250 μg/mL for times ranging from 1 to 24 h. An equivalent amount of CaCl2 solution (based on calcium content) was also prepared for comparison. Next, 10 μL of WST-1 solution (Roche) was added to each well, and cells were further incubated for 4 h. Absorbance was then measured using a plate reader at 440 nm (Dynex Technologies, Chantilly, VA, USA). Cells incubated in the absence of particles were used as the control. The experiment was repeated three times on three separate days.
Intracellular ROS levels were monitored using a peroxide-sensitive fluorescent probe, carboxy-2ʹ,7ʹ-dichlorofluorescein diacetate (H2DCFDA, Molecular Probes, Eugene, OR, USA), according to the manufacturer’s guidelines. Briefly, cells (5 × 103/100 μL) were incubated with the particles or an equivalent amount of CaCl2 (based on calcium content) for 24 h, washed with PBS, collected by centrifugation, and incubated with 40 μM carboxy-H2DCFDA for 60 min at 37 °C. After washing with PBS, dichlorofluorescein fluorescence was immediately measured using a fluorescence microplate reader (SpectraMax® M3, Molecular Devices, Silicon Valley, CA, USA), and excitation and emission wavelengths were 490 and 530 nm, respectively. Cells not treated with particles were used as the control. The experiment was repeated three times on three separate days.
The release of LDH was monitored with the CytoTox 96 Non-Radioactive Cytotoxicity assay (Promega, Madison, WI, USA). Cells (5 × 104 cells/1 mL) were incubated with 250 μg/mL CaCO3 materials or an equivalent amount of CaCl2 (based on calcium content) for times ranging from 1 h to 24 h. Then, the plates were centrifuged, and aliquots (50 μL) of cell culture medium were collected from each well and placed in new microtiter plates. Then, 50 μL of substrate solution was added to each well and the plates were further incubated for 30 min at room temperature. Finally, after adding the 50 μL of stop solution, the absorbance at 490 nm was measured with a microplate reader (SpectraMax® M3, Molecular Devices, Silicon Valley, CA, USA). Cytotoxicity is expressed relative to the basal LDH release from untreated control cells. The experiment was repeated three times on three separate days.
Cells (1 × 106/mL) were incubated overnight under the standard condition as described above, then replaced with fresh medium containing 250 μg/mL CaCO3 materials or an equivalent amount of CaCl2 (based on calcium content) for 2 h. Cells were then washed three times with PBS and treated with 5 mM EDTA for 40 s to remove particles not taken up by the cells. Higher EDTA concentration for more prolonged time was found to cause membrane damage. After washing three times with PBS, cells were harvested by scraping and centrifuged. The cell pellets thus obtained were digested in 3 mL of ultrapure nitric acid, treated with 0.5 mL of H2O2, and heated at about 160 °C. Each mixture was heated until the samples were completely digested. The remaining solution was then removed by heating until the solutions were colorless and clear. The solution were finally diluted to 5 mL with D.D.W. and filtered with 0.45 μm. Calcium concentrations were determined by ICP-AES (JY2000 Ultrace; HORIBA Jobin Yvon, Stow, MA, USA). Cells incubated in the absence of particles were used as controls.
In order to determine the role of energy-dependent endocytosis in CaCO3 uptake, the uptake experiment was also performed at 4 °C and CaCO3 uptake was analyzed by ICP-AES in the same manner. On the other hand, intracellular Ca2+ levels resulted from uptake of CaCO3 materials or an equivalent amount of CaCl2 (based on calcium content) were monitored with Calcium Green™−1 probe (Life Technologies, Carsbad, CA, USA). Cells (5 × 104 cells/1 mL) were incubated with 250 μg/mL CaCO3 materials or an equivalent amount of CaCl2 (based on calcium content) for 60 min in the presence of 10 μM probe. The fluorescence was immediately measured using a fluorescence microplate reader (SpectraMax® M3, Molecular Devices, Silicon Valley, CA, USA), and excitation and emission wavelengths were 506 nm and 531 nm, respectively. Cells not treated with particles were used as the control. All experiments were repeated three times on three separate days.
For mechanistic study on intestinal transport, an in vitro model of human intestinal FAE was prepared according to the protocol developed by des Rieux et al. [28,29]. Human intestinal epithelial Caco-2 cells were purchased from the Korean Cell Line Bank (Seoul, Korea) and grown in DMED supplemented with 10% fetal bovine serum, 1% non-essential amino acids, 1% L-glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin under the standard condition as described above. Briefly, Transwell® polycarbonate inserts (Corning Costar, New York, NY, USA) were coated with Matrigel™ basement membrane matrix (Becton Dicknson, Bedford, MA, USA), prepared in pure DMEM, and then placed at room temperature for 1 h. Supernatants were removed and inserts were washed with DMEM. Caco-2 cells (5 × 105 cells) were grown on the upper insert side and incubated for 14 days. Then, non-adherent human Burkitt’s lymphoma Raji B cells (5 × 105 cells, Korean Cell Line Bank, Seoul, Korea) in the same medium were added to the basolateral insert compartment, and the co-cultures were maintained for 5 days. CaCO3 materials (250 μg/mL) or an equivalent amount of CaCl2 (based on calcium content) were prepared in Hank’s balanced salt solution buffer, and apical medium of the cell monolayers were replaced by a particle suspension and incubated for 6 h. Basolateral solutions were then sampled and the concentration of transported particles were estimated by measuring total calcium levels with ICP-AES as described above. The experiment was repeated three times on three separate days.
Six female rats per group were administered a single dose of 250 mg/kg of the three CaCO3 or an equivalent amount CaCl2 by oral gavage; controls (n = 6) received an equivalent volume of 0.9% saline. All animal experiments were performed after obtaining approval from the Animal and Ethics Review Committee of Seoul Women’s University. Body weight changes, behaviors, and symptoms were carefully recorded daily after treatment. To determine plasma calcium concentrations, blood samples were collected via a tail vein at 0, 0.25, 0.5, 1, 2, 4, and 6 h of post-oral administration. Blood samples were centrifuged at 3000× g for 15 min at 4 °C to obtain plasma. The following pharmacokinetic parameters were estimated using Kinetica version 4.4 (Thermo Fisher Scientific, Waltham, MA, USA): maximum concentration (Cmax), time to maximum concentration (Tmax), area under the plasma concentration-time curve (AUC), half-life (T1/2), and mean residence time (MRT). The plasma samples were quantitatively analyzed by ICP-AES as described above.
The statistical analysis was performed using the Student’s t-test for unpaired data, and one-way analysis of variance (Tukey’s test, version 11.0) was conducted using SAS software (SAS Institute, Cary, NC, USA) to determine the significance of differences between experimental groups. All results are presented as the mean ± standard deviation and p < 0.05 were considered to be statistically significant.
We investigated the effect of particle size (bulk versus nano) of food grade CaCO3, produced by grinding sea shells, on cytotoxicity, cellular uptake, intestinal transport, and in vivo oral absorption. A comparative study with bottom-up synthesized reagent grade SS CaCO3 nanoparticles or Ca2+ ions was also performed. The physicochemical characterization revealed that food nano CaCO3 was well produced with an average size of ~100 nm, but it had morphological rough surface compared to SS CaCO3. Both food nano CaCO3 and SS CaCO3 nanoparticles exhibited slightly high toxicity compared to food bulk CaCO3 in terms of ROS generation and LDH release. Cellular uptake behaviors of CaCO3 particles were different from Ca2+ ions, showing significantly increased uptake and energy-dependent endocytic pathways, especially for nano-sized particles. The M cells were determined to be an important intestinal transport mechanism of CaCO3 nanoparticles, in a similar manner as Ca2+ ions did, implying that CaCO3 nanoparticles can be efficiently transcytosed across the intestinal epithelium. In vivo biokinetic study demonstrated more rapid absorption of CaCO3 nanoparticles than food bulk CaCO3, but total absorption efficiency was not affected by particle size. Biokinetics of CaCO3 particles were different from Ca2+ ions, suggesting that the biological fate of CaCO3 particles was primarily a particulate form, regardless of particle size, showing a slower absorption rate and lower total oral absorption efficacy than Ca2+ ions. These results will be useful to understand and predict potential toxicity and oral efficacy of food grade nanoparticles.
This work was financially supported the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (NO. 2013R1A1A3009283), and by Nano Material Technology Development Program (NO. 2014M3A7B6020163) of MSIP/NRF.
Soo-Jin Choi designed the experiments and drafted the manuscript. Mi-Kyung Kim carried out all physicochemical characterizations, cell culture, cytotoxicity, cellular uptake, and animal experiments. Jeong-A Lee performed animal and intestinal transport experiments. Mi-Rae Jo conducted the uptake mechanism study and statistical analysis. Min-Kyu Kim carried out microscopic measurements to evaluate surface roughness. Hyoung-Mi Kim contributed to physicochemical characterization and interpretation. Jae-Min Oh designed and wrote the manuscript on material characterization. Nam Woong Song helped to design and draft the manuscript.
The authors declare no conflict of interest.