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Immune thrombocytopenia (ITP) is a common autoimmune bleeding disorder. Despite considerable investigation, the pathogenesis of ITP remains incompletely understood, and for many patients, effective therapy is still unavailable. Using murine models and in vitro studies of human blood samples, we recently identified a novel Fc-independent platelet clearance pathway, whereby antibody-mediated desialylated platelets can be cleared in the liver via asialoglycoprotein receptors, leading to decreased response to standard first-line therapies targeting Fc-dependent platelet clearance. Here, we evaluated the significance of this finding in 61 ITP patients through correlation of levels of platelet desialylation with the efficacy of first-line therapies. We found that desialylation levels between different responses to treatment groups were statistically significant (p<0.01). Importantly, correlation analysis indicated response to treatment and platelet desialylation were related (p<0.01), whereby non-responders had significantly higher levels of platelet desialylation. Interestingly, we also found secondary ITP and certain non-ITP thrombocytopenias also exhibited significant platelet desialylation compared to healthy controls. These findings designate platelet desialylation as an important biomarker in determining response to standard treatment for ITP. Furthermore, we show for the first time platelet desialylation in other non-ITP thrombocytopenias, which may have important clinical implications and deserve further investigation.
The online version of this article (doi:10.1186/s13045-017-0413-3) contains supplementary material, which is available to authorized users.
Immune thrombocytopenia (ITP) is a common clinical bleeding disorder characterized by an immune-mediated clearance of autologous platelets, predominantly through autoantibodies targeting platelet surface receptors GPIIbIIIa and/or GPIb-IX and clearance by phagocytic cells in the reticuloendothelial system via Fcγ-receptors [1–4]. Low platelet counts place ITP patients at risk for severe bleeding including fatal intracranial hemorrhage. Most therapies for ITP including first-line corticosteroids and immunoglobulin G (IVIG), and last resort splenectomy, mainly target the Fc-dependent clearance pathway via blocking/attenuating Fc-Fcγ-R interaction or removal of putative site of platelet clearance . However, the pathogenesis and mechanisms of therapies remain poorly understood and around 15–20% of ITP patients are inexplicably refractory to first-line therapies, and around 10% are refractory to splenectomy [5, 6]. In recent years, murine models and large cohort human studies report antibody specificity (i.e., anti-GPIIbIIIa versus anti-GPIb-IX) may play a significant role in dictating response to therapy in ITP [7–9]; whereby presence of anti-GPIb-IX antibodies results in decreased response to corticosteroids and IVIG [7–9]. Most recently, we reported that anti-GPIbα and some anti-GPIIbIIIa antibodies in humans induced platelet desialylation leading to Fc-independent platelet clearance in the liver via hepatic asialoglycoprotein Ashwell-Morell receptors , suggesting antibody-mediated desialylation may be one of the underlying mechanisms behind resistance to standard ITP therapies [8, 9, 11].
In the present study, we sought to address whether increased platelet desialylation was correlated with decreased response to treatment in ITP patients (Additional file 1: Supplementary Material). The platelets of randomly and consecutively enrolled 61 patients diagnosed with primary ITP were tested for desialylation prior to the indicated treatments (Table 1). Fluorescein-conjugated lectins Ricinus communis agglutinin I (RCA-1) and Erythrina cristagalli lectin (ECL) were used to detect desialylated galactose and β-GlcNAc residues via flow cytometry. We found the platelets of ITP patients had significantly higher desialylation as measured by both RCA-1 and ECL binding compared to those of healthy blood donors (p<0.05) (Fig. 1). The 61 ITP patients subsequently underwent standard first-line therapy independent of platelet desialylation and MAIPA assays. After 1 month of treatment, there were 26 complete responders (CR), 21 responders (R), and 14 non-responders (NR) (Table 1). Retrospective data analysis using Kruskal-Wallis rank sum test revealed NR patients had significantly higher platelet desialylation, as compared to the CR and R groups (p<0.01). Correlation analysis indicated that efficacy and the desialylation level are related (RCA-1 r=0.395, p<0.01; ECL r=0.391, p<0.01). The higher desialylation, the poorer the efficacy of therapy observed.
To test whether the presence of anti-GPIbα antibodies is associated with the platelet desialylation, we detected antibody using MAIPA in the available 33 patient samples collected prior to treatment. We observed a two- to sixfold increased platelet desialylation in patients with anti-GPIbα antibodies (n=9) compared to that in patients with anti-GPIIbIIIa (n=14) or without detectable antibodies (n=10) (Table 1 and Fig. 1B). However, statistical significance was not reached, which is likely due to small sample size. Future larger studies should be useful in determining direct correlation between anti-GPIbα antibody positivity with platelet desialylation.
Interestingly, we also observed significant platelet desialylation in patients with non-ITP thrombocytopenias including connective tissue diseases (CTD), myelodysplastic syndrome (MDS), and aplastic anemia (AA) (p<0.001) but not acute myeloid leukemia (AML) compared to healthy controls (Table 1 and Fig. 1B). Notably, although RCA-1 and ECL measures different types of deglycosylation, we did not observe significant difference between these two assays, suggesting either of them can be used for the potential diagnosis and prognosis.
In summary, our data demonstrates for the first time that the higher level of platelet desialylation is correlated with non-response to the first-line ITP therapies (likely also splenectomies; Additional file 1: Supplementary Material). These findings not only suggest that platelet desialylation is a useful biomarker in predicting response to treatment in clinical ITP but positions sialidase inhibitors, such as Tamiflu , as a potential novel therapeutic in the treatment of ITP as well as other thrombocytopenias.
The authors would like to thank Mr. Thomas McKeown, Miss Xun Fu, and Miss Jade Sullivan for editing the manuscript.
This work was supported by the Canadian Institutes of Health Research (MOP 97918, MOP 119540, MOP 119551); Canadian Blood Services-Canadian Institutes of Health Research partnership fund (CIHR-BUC201403-HN-326400); by the Special Research Funding for the Doctoral Program (NO. 20103420110001), Ministry of Education, People’s Republic of China; and by the Foundation of the Second Affiliated Hospital and the Hematological Research Center, Anhui Medical University, People’s Republic of China. June Li is a recipient of Ph.D. Graduate Fellowship from Canadian Blood Services. Miao Xu is a recipient of the State Scholarship Fund from the China Scholarship Council and Ontario Trillium Scholarship, Canada.
Data sharing is not applicable to this article as no datasets were generated or analysed during the current study.
LT planned and carried out the experiments, analyzed the data, and prepared the manuscript. QZ supervised the clinical studies and analyzed the data. LJ and MX analyzed the data and prepared the manuscript. JW, YP, HW, QT, and YC carried out the experiments and analyzed the data. AJGJ, JP, and MH joined the international collaboration group meetings, analyzed the data and contributed to the preparation of manuscript. HN and ZZ (PIs) supervised the research, analyzed the data, and prepared the manuscript. LT and QZ contributed equally to this work and should be acknowledged as co-first authors. Both Dr. HN and Dr. ZZ should be acknowledged as co-corresponding authors. All authors read and approved the final manuscript.
The contact information for the corresponding authors:
Heyu Ni, M.D.; Ph.D, Professor, Department of Laboratory Medicine and Pathobiology, Department of Medicine, and Department of Physiology, University of Toronto; Scientist of Canadian Blood Services Centre for Innovation; Platform Director for Hematology, Cancer and Immunological Diseases, St. Michael’s Hospital, Room 420, LKSKI - Keenan Research Centre, 209 Victoria Street, Toronto, Ontario, M5B 1W8, CANADA. Tel: 1-416-847-1738; Email: email@example.com
Zhimin Zhai, M.D.; PhD. Professor, Director of Department of Hematology, The Second Affiliated Hospital and the Hematological Research Center, Anhui Medical University, Hefei 230601, China. Tel: 86-138-5514-7434; Email: firstname.lastname@example.org
The authors declare that they have no competing interests.
The study has been approved by the Ethic Board of Anhui Medical University (No. 20131038). Patients in this study all signed informed consents to clinical data use at enrolment.
Heyu Ni, Phone: 1-416-847-1738, Email: ac.hms@hin.
Zhimin Zhai, Phone: 86-138-5514-7434, Email: moc.361@988mzzz.