Search tips
Search criteria 


Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. 1978 March; 25(3): 878–887.
PMCID: PMC525982

Physical map of the origin of defective DNA in herpes simplex virus type 1 DNA.


The origin of defective DNA (dDNA) of the Patton strain of herpes simplex virus type 1 (HSV-1) was physically mapped with BamHI in the parental DNA. The dDNA obtained from virus passaged at high multiplicities of infection was resistant to cleavage with HindIII, whereas digestion with EcoRI yielded a cluster of fragments 5.4 to 5.7 megadaltons (Mdal) in size. Cleavage with BamHI gave a cluster of fragments 2.6 to 3.2 Mdal in size, plus two homogeneous, comigrating 1-Mdal fragments. One of the latter fragments contained the single EcoRI site approximately 65 base pairs from one end. Hybridization of in vitro labeled dDNA probe to EcoRI, HindIII, BamHI, and Hpa I digests of nondefective HSV-1 DNA demonstrated that, in addition to the S-region terminal repeat, only one end of the S region was involved in the generation of this class of dDNA. Thus, the dDNA probe did not hybridize to either the S region 3.0-Mdal HindIIIN fragment or a 3.0-Mdal BamHI fragment of the adjacent 8.7-Mdal HindIIIG fragment, but did hybridize to four BamHI fragments of HindIII G (approximately 5.7 Mdal). The cluster of 2.6- to 3.2-Mdal fragments obtained with BamHI digestion of dDNA appears to represent a novel junction between the termination of dDNA adjacent to the 3.0-Mdal BamHI fragment in HindIII G and the 2.0- to 2.3-Mdal BamHI fragment terminal in HSV-1 DNA.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Becker Y, Dym H, Sarov I. Herpes simplex virus DNA. Virology. 1968 Oct;36(2):184–192. [PubMed]
  • Bronson DL, Dreesman GR, Biswal N, Benyesh-Melnick M. Defective virions of herpes simplex viruses. Intervirology. 1973;1(3):141–153. [PubMed]
  • Clements JB, Cortini R, Wilkie NM. Analysis of herpesvirus DNA substructure by means of restriction endonucleases. J Gen Virol. 1976 Feb;30(2):243–256. [PubMed]
  • Frenkel N, Jacob RJ, Honess RW, Hayward GS, Locker H, Roizman B. Anatomy of herpes simplex virus DNA. III. Characterization of defective DNA molecules and biological properties of virus populations containing them. J Virol. 1975 Jul;16(1):153–167. [PMC free article] [PubMed]
  • Frenkeĺ N, Locker H, Batterson W, Hayward GS, Roizman B. Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component. J Virol. 1976 Nov;20(2):527–531. [PMC free article] [PubMed]
  • Graham BJ, Ludwig H, Bronson DL, Benyesh-Melnick M, Biswal N. Physicochemical properties of the DNA of herpes viruses. Biochim Biophys Acta. 1972 Jan 18;259(1):13–23. [PubMed]
  • Hayward GS, Frenkel N, Roizman B. Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc Natl Acad Sci U S A. 1975 May;72(5):1768–1772. [PubMed]
  • Hayward GS, Jacob RJ, Wadsworth SC, Roizman B. Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4243–4247. [PubMed]
  • Kieff ED, Bachenheimer SL, Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. [PMC free article] [PubMed]
  • Maniatis T, Kee SG, Efstratiadis A, Kafatos FC. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. [PubMed]
  • Mulder C, Sharp PA, Delius H, Pettersson U. Specific fragmentation of DNA of adenovirus serotypes 3, 5, 7, and 12, and adeno-simian virus 40 hybrid virus Ad2+ND1 by restriction endonuclease R.EcoRI. J Virol. 1974 Jul;14(1):68–77. [PMC free article] [PubMed]
  • Robey WG, Graham BJ, Harris CL, Madden MJ, Pearson GR, Vande Woude GF. Persistent herpes simplex virus infections established in two Burkitt lymphoma derived cell lines. J Gen Virol. 1976 Jul;32(1):51–62. [PubMed]
  • Schröder CH, Stegmann B, Lauppe HF, Kaerner HC. An unusual defective genotype derived from herpes simplex virus strain ANG. Intervirology. 1975;6(4-5):270–284. [PubMed]
  • Sheldrick P, Berthelot N. Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):667–678. [PubMed]
  • Simonds JA, Robey WG, Graham BJ, Oie H, Vande Woude GF. Purification of herpesvirus saimiri and properties of the viral DNA. Arch Virol. 1975;49(2-3):249–259. [PubMed]
  • Skare J, Summers WC. Structure and function of herpesvirus genomes. II. EcoRl, Sbal, and HindIII endonuclease cleavage sites on herpes simplex virus. Virology. 1977 Feb;76(2):581–595. [PubMed]
  • Skare J, Summers WP, Summers WC. Structure and function of herpesvirus genomes. I. comparison of five HSV-1 and two HSV-2 strains by cleavage their DNA with eco R I restriction endonuclease. J Virol. 1975 Apr;15(4):726–732. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Steinhart WL, Grafstrom RH, Hill CW. Terminal fragments of herpes simplex virus DNA produced by restriction endonuclease. Biochem Biophys Res Commun. 1975 Nov 17;67(2):556–561. [PubMed]
  • Thuring RW, Sanders JP, Borst P. A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem. 1975 May 26;66(1):213–220. [PubMed]
  • Wadsworth S, Hayward GS, Roizman B. Anatomy of herpes simplex virus DNA. V. Terminally repetitive sequences. J Virol. 1976 Feb;17(2):503–512. [PMC free article] [PubMed]
  • Wadsworth S, Jacob RJ, Roizman B. Anatomy of herpes simplex virus DNA. II. Size, composition, and arrangement of inverted terminal repetitions. J Virol. 1975 Jun;15(6):1487–1497. [PMC free article] [PubMed]
  • Wagner M, Skare J, Summers WC. Analysis of DNA of defective herpes simplex virus type 1 by restriction endonuclease cleavage and nucleic acid hybridization. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):683–686. [PubMed]
  • Wilkie NM. Physical maps for Herpes simplex virus type 1 DNA for restriction endonucleases Hind III, Hpa-1, and X. bad. J Virol. 1976 Oct;20(1):222–233. [PMC free article] [PubMed]
  • Wilkie NM, Cortini R. Sequence arrangement in herpes simplex virus type 1 DNA: identification of terminal fragments in restriction endonuclease digests and evidence for inversions in redundant and unique sequences. J Virol. 1976 Oct;20(1):211–221. [PMC free article] [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)