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J Clin Pathol. 1990 December; 43(12): 1026–1028.
PMCID: PMC502979

Monoclonal antibody B72.3 in benign breast lesions.


It has been suggested that the monoclonal antibody B72.3 may be useful as a diagnostic tool in fine needle aspirates of breast masses because it recognises "tumour associated glycoprotein (TAG)-72". The antigen was sought in paraffin wax sections of 43 normal and benign breast biopsy specimens, using the avidin-biotin complex technique, to assess the extent of its presence in non-malignant tissue. Strong focal staining was seen in 21 (49%) cases. In 29 cases of fibrocystic change staining was present in 17 (59%). All areas of apocrine metaplasia were positive, as well as a few normal ducts and acini and occasional areas of adenosis. Focal positivity was present in five out of 12 foci of ductal epithelial hyperplasia and in three out of seven radial scars. Staining was absent in two areas of lobular hyperplasia, three areas of sclerosing adenosis, and in a focus of lactational change. Focal positivity was also seen in two out of five fibroadenomas and in two out of three intraduct papillomas. Five normal subareolar sections and a section of normal lactating breast were negative. It is concluded that B72.3 monoclonal antibody can show focal reactivity with a variety of normal and benign epithelial mammary structures, and it is doubtful that its use would be of any help in differentiating benign from malignant cells in fine needle aspirates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Colcher D, Hand PH, Nuti M, Schlom J. A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc Natl Acad Sci U S A. 1981 May;78(5):3199–3203. [PubMed]
  • Johnson VG, Schlom J, Paterson AJ, Bennett J, Magnani JL, Colcher D. Analysis of a human tumor-associated glycoprotein (TAG-72) identified by monoclonal antibody B72.3. Cancer Res. 1986 Feb;46(2):850–857. [PubMed]
  • Muraro R, Kuroki M, Wunderlich D, Poole DJ, Colcher D, Thor A, Greiner JW, Simpson JF, Molinolo A, Noguchi P, et al. Generation and characterization of B72.3 second generation monoclonal antibodies reactive with the tumor-associated glycoprotein 72 antigen. Cancer Res. 1988 Aug 15;48(16):4588–4596. [PubMed]
  • Sheer DG, Schlom J, Cooper HL. Purification and composition of the human tumor-associated glycoprotein (TAG-72) defined by monoclonal antibodies CC49 and B72.3. Cancer Res. 1988 Dec 1;48(23):6811–6818. [PubMed]
  • Johnston WW, Szpak CA, Lottich SC, Thor A, Schlom J. Use of a monoclonal antibody (B72.3) as a novel immunohistochemical adjunct for the diagnosis of carcinomas in fine needle aspiration biopsy specimens. Hum Pathol. 1986 May;17(5):501–513. [PubMed]
  • Lundy J, Lozowski M, Mishriki Y. Monoclonal antibody B72.3 as a diagnostic adjunct in fine needle aspirates of breast masses. Ann Surg. 1986 Apr;203(4):399–402. [PubMed]
  • Kline TS, Lundy J, Lozowski M. Monoclonal antibody B72.3. An adjunct for evaluation of suspicious aspiration biopsy cytology from the breast. Cancer. 1989 Jun 1;63(11):2253–2256. [PubMed]
  • Castagna M, Nuti M, Squartini F. Mammary cancer antigen recognized by monoclonal antibody B72.3 in apocrine metaplasia of the human breast. Cancer Res. 1987 Feb 1;47(3):902–906. [PubMed]
  • Tavassoli FA, Jones MW, Majeste RM, Bratthauer GL, O'Leary TJ. Immunohistochemical staining with monoclonal Ab B72.3 in benign and malignant breast disease. Am J Surg Pathol. 1990 Feb;14(2):128–133. [PubMed]
  • Page DL. Cancer risk assessment in benign breast biopsies. Hum Pathol. 1986 Sep;17(9):871–874. [PubMed]
  • Wellings SR, Alpers CE. Apocrine cystic metaplasia: subgross pathology and prevalence in cancer-associated versus random autopsy breasts. Hum Pathol. 1987 Apr;18(4):381–386. [PubMed]

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