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J Clin Pathol. 1990 August; 43(8): 654–656.
PMCID: PMC502648

Localisation of CD10 to biliary canaliculi by immunoelectron microscopical examination.


Common acute lymphoblastic leukaemia antigen (CALLA) was first characterised in lymphoid leukaemic cells. The antigen is present in different stages of lymphoid cell differentiation as well as in subsets of myeloid cells, and further studies have also shown its presence in non-lymphoid tissues. The recent cloning and sequencing of the gene permitted deduction of its amino acid sequence which is identical with the human membrane-associated enzyme, neutral endopeptidase. Strong immunostaining for CALLA was detected in the human liver with a canalicular pattern. Immunoelectron microscopy also confirmed that the antigen was localised only in the area of the bile canaliculi. Although the function of neutral endopeptidase in the canaliculi is unknown, this antigen may prove useful in the study of biliary function and diseases.

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Selected References

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  • Greaves MF, Hariri G, Newman RA, Sutherland DR, Ritter MA, Ritz J. Selective expression of the common acute lymphoblastic leukemia (gp 100) antigen on immature lymphoid cells and their malignant counterparts. Blood. 1983 Apr;61(4):628–639. [PubMed]
  • Cossman J, Neckers LM, Leonard WJ, Greene WC. Polymorphonuclear neutrophils express the common acute lymphoblastic leukemia antigen. J Exp Med. 1983 Mar 1;157(3):1064–1069. [PMC free article] [PubMed]
  • Braun MP, Martin PJ, Ledbetter JA, Hansen JA. Granulocytes and cultured human fibroblasts express common acute lymphoblastic leukemia-associated antigens. Blood. 1983 Apr;61(4):718–725. [PubMed]
  • Metzgar RS, Borowitz MJ, Jones NH, Dowell BL. Distribution of common acute lymphoblastic leukemia antigen in nonhematopoietic tissues. J Exp Med. 1981 Oct 1;154(4):1249–1254. [PMC free article] [PubMed]
  • Quackenbush EJ, Gougos A, Baumal R, Letarte M. Differential localization within human kidney of five membrane proteins expressed on acute lymphoblastic leukemia cells. J Immunol. 1986 Jan;136(1):118–124. [PubMed]
  • Gusterson BA, Monaghan P, Mahendran R, Ellis J, O'Hare MJ. Identification of myoepithelial cells in human and rat breasts by anti-common acute lymphoblastic leukemia antigen antibody A12. J Natl Cancer Inst. 1986 Aug;77(2):343–349. [PubMed]
  • Carrel S, Schmidt-Kessen A, Mach JP, Heumann D, Girardet C. Expression of common acute lymphoblastic leukemia antigen (CALLA) on human malignant melanoma cell lines. J Immunol. 1983 May;130(5):2456–2460. [PubMed]
  • Carrel S, De Tribolet N, Gross N. Expression of HLA-DR and common acute lymphoblastic leukemia antigens on glioma cells. Eur J Immunol. 1982 Apr;12(4):354–357. [PubMed]
  • Seshadri R, Matthews C, Henderson DW, Zola H, Callen D. A human retinoblastoma cell line expressing the common acute lymphoblastic leukemia antigen and displaying an unusual chromosome abnormality. Cancer Genet Cytogenet. 1986 Feb 15;20(3-4):345–354. [PubMed]
  • Pesando JM, Tomaselli KJ, Lazarus H, Schlossman SF. Distribution and modulation of a human leukemia-associated antigen (CALLA). J Immunol. 1983 Oct;131(4):2038–2045. [PubMed]
  • Brox AG, Lavallee MC, Arseneau J, Langleben A, Major PP. Expression of common acute lymphoblastic leukemia-associated antigen on germ cell tumor. Am J Med. 1986 Jun;80(6):1249–1252. [PubMed]
  • Ritz J, Pesando JM, Notis-McConarty J, Lazarus H, Schlossman SF. A monoclonal antibody to human acute lymphoblastic leukaemia antigen. Nature. 1980 Feb 7;283(5747):583–585. [PubMed]
  • Newman RA, Sutherland R, Greaves MF. The biochemical characterization of a cell surface antigen associated with acute lymphoblastic leukemia and lymphocyte precursors. J Immunol. 1981 May;126(5):2024–2030. [PubMed]
  • Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. [PubMed]
  • Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford KA, Stein H, Mason DY. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. [PubMed]
  • Wahab ZA, Wright GL., Jr Monoclonal antibody (anti-Leu 7) directed against natural killer cells reacts with normal, benign and malignant prostate tissues. Int J Cancer. 1985 Dec 15;36(6):677–683. [PubMed]
  • Voigt JJ, Vinel JP, Caverivière P, Pradère B, Chittal S, al Saati T, Calès P, Delsol G. Diagnostic immunohistochimique des localisations hépatiques des hémopathies lymphoïdes malignes. Etude de 80 cas. Gastroenterol Clin Biol. 1989 Apr;13(4):343–352. [PubMed]
  • Shipp MA, Richardson NE, Sayre PH, Brown NR, Masteller EL, Clayton LK, Ritz J, Reinherz EL. Molecular cloning of the common acute lymphoblastic leukemia antigen (CALLA) identifies a type II integral membrane protein. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4819–4823. [PubMed]
  • Letarte M, Vera S, Tran R, Addis JB, Onizuka RJ, Quackenbush EJ, Jongeneel CV, McInnes RR. Common acute lymphocytic leukemia antigen is identical to neutral endopeptidase. J Exp Med. 1988 Oct 1;168(4):1247–1253. [PMC free article] [PubMed]
  • Ishida M, Ogawa M, Kosaki G, Mega T, Ikenaka T. Purification and characterization of the neutral endopeptidase from human kidney. J Biochem. 1983 Jul;94(1):17–24. [PubMed]
  • Lorkowski G, Zijderhand-Bleekemolen JE, Erdös EG, von Figura K, Hasilik A. Neutral endopeptidase-24.11 (enkephalinase). Biosynthesis and localization in human fibroblasts. Biochem J. 1987 Dec 1;248(2):345–350. [PubMed]
  • Johnson AR, Ashton J, Schulz WW, Erdös EG. Neutral metalloendopeptidase in human lung tissue and cultured cells. Am Rev Respir Dis. 1985 Sep;132(3):564–568. [PubMed]
  • Johnson AR, Skidgel RA, Gafford JT, Erdös EG. Enzymes in placental microvilli: angiotensin I converting enzyme, angiotensinase A, carboxypeptidase, and neutral endopeptidase ("enkephalinase"). Peptides. 1984 Jul-Aug;5(4):789–796. [PubMed]
  • Erdös EG, Schulz WW, Gafford JT, Defendini R. Neutral metalloendopeptidase in human male genital tract. Comparison to angiotensin I-converting enzyme. Lab Invest. 1985 Apr;52(4):437–447. [PubMed]
  • Connelly JC, Skidgel RA, Schulz WW, Johnson AR, Erdös EG. Neutral endopeptidase 24.11 in human neutrophils: cleavage of chemotactic peptide. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8737–8741. [PubMed]
  • Schwartz JC, Malfroy B, De La Baume S. Biological inactivation of enkephalins and the role of enkephalin-dipeptidyl-carboxypeptidase ("enkephalinase") as neuropeptidase. Life Sci. 1981 Oct 26;29(17):1715–1740. [PubMed]
  • LeBien TW, McCormack RT. The common acute lymphoblastic leukemia antigen (CD10)--emancipation from a functional enigma. Blood. 1989 Feb 15;73(3):625–635. [PubMed]
  • Painter RG, Dukes R, Sullivan J, Carter R, Erdös EG, Johnson AR. Function of neutral endopeptidase on the cell membrane of human neutrophils. J Biol Chem. 1988 Jul 5;263(19):9456–9461. [PubMed]

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