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J Natl Cancer Inst. 2016 August; 108(8): djw111.
Published online 2016 July 4. doi:  10.1093/jnci/djw111
PMCID: PMC5017949

RE: RNA Disruption Assay as a Biomarker of Pathological Complete Response in Neoadjuvant Trastuzumab-Treated Human Epidermal Growth Factor Receptor 2–Positive Breast Cancer

In Pritzker et al. (1), RNA disruption assay (RDA) was found to be a novel, early, on-treatment assay that has potential for clinical utility in response-guided primary systemic breast cancer (BC) therapy. This assay quantifies early chemotherapy-induced RNA disruption in breast tumors and has shown clinical utility by predicting pathological complete response (pCR) rates in the neoadjuvant treatment of BC patients (1–4). High RDA scores above 7 are associated with a higher chance of pCR than lower RDA scores. RDA has not yet been validated specifically in an independent cohort of human epidermal growth factor receptor 2 (HER2)–positive BC.

TCHL (NCT01485926) was a phase II neo-adjuvant study assessing TCH (docetaxel, carboplatin, and trastuzumab) and TCHL (TCH and lapatinib) in early-stage HER2-positive BC (5). The study’s primary endpoint was to compare rates of pCR between the TCH and TCHL arms. Of the 78 patients enrolled, 23 had a core biopsy taken by an interventional radiologist, 20 days post-cycle 1 of either TCH/TCHL therapy.

These samples potentially offer a unique insight into the molecular and pathological changes that tumors undergo during the patients’ initial treatment and how they relate to the final pCR status of patients. Pathological review of these samples (Figure 1, A and B) indicates that in 10 patients who later achieved a pCR at surgery, five had no tumor present in their on-treatment tumor biopsy sample and the average tumor content in those patients who achieved pCR was 10% ± 15%. In patients who either had a partial response or no response (n = 13 evaluable patient samples), the average tumor content in the on-treatment biopsy sample was 60% ± 23%, which was higher than that observed in the pCR samples (P = .000001). This interesting finding highlights the immediate impact that trastuzumab has on tumor content in HER2-positive BC patients.

Figure 1.
Biopsy cores taken 20 days after the initiation of neoadjuvant therapy by a radiologist were embedded in optical coherence tomography and the samples were cryo-sectioned. A single 3 µM section was taken for haematoxylin and eosin (H&E) ...

In this cohort, RDA scores of greater than 7 in the on-treatment biopsy sample indicated a higher chance of pCR in response to TCH or TCHL chemotherapy (6 of 7 patients with an RDA >7 subsequently achieved a pCR vs 1 of 10 patients with a score <7). Figure 1C indicates that those patients who had a pCR (n = 10) had an average RDA score of 10.2 ± 5.1, which was higher than in those patients who had a partial or no response at subsequent surgery (n = 10; RDA score of 5.4 ± 2.2, P = .025).

Our results support the work of Pritzker et al. (1) and demonstrate the benefit of obtaining core tumor biopsies after cycle 1 of neoadjuvant treatment in HER2-positive BC patients for further study of the clinical utility of RDA. Pathological analysis of these samples will demonstrate the effect of treatment, and the use of RDA score may allow for easy and robust stratification of patients into two groups with and without a high likelihood of pCR at subsequent surgery. The RDA score may be a useful early prognostic and predictive biomarker of the likelihood of later pCR, with the potential to guide subsequent neoadjuvant treatment in an attempt to optimize pCR rates.

Funding

The trial sponsor was Ireland Clinical Oncology Research Group, which received funding from GlaxoSmithKline. This work was also supported by the Irish Cancer Society Collaborative Cancer Research Centre, BREAST-PREDICT Grant, CCRC13GAL (http://www.breastpredict.com), the Health Research Board (HRA/POR2012/054), and the North Eastern Cancer Research and Education Trust, and the Science Foundation Ireland funded Molecular Therapeutics for Cancer Ireland (08-SRC-B1410).

Notes

We would like to acknowledge the study participants, the All Ireland Clinical Oncology Research Group (ICORG) site research staff for coordinating sample collection and Ausra Teiserskiene at ICORG Group Central Office for central coordination of study samples.

References

1. Pritzker K, Pritzker L, Generali D., et al. RNA Disruption and Drug Response in Breast Cancer Primary Systemic Therapy. J Natl Cancer Inst Monogr. 2015;2015(51):76–80. [PubMed]
2. Parissenti AM, Guo B, Pritzker LB., et al. Tumor RNA disruption predicts survival benefit from breast cancer chemotherapy. Breast Cancer Res Treat. 2015;153(1):135–144. [PubMed]
3. Foroni C, Milan M, Strina C., et al. Pure anti-tumor effect of zoledronic acid in naive bone-only metastatic and locally advanced breast cancer: proof from the "biological window therapy." Breast Cancer Res Treat. 2014;144(1):113–121. [PubMed]
4. Parissenti AM, Chapman JA, Kahn HJ., et al. Association of low tumor RNA integrity with response to chemotherapy in breast cancer patients. Breast Cancer Res Treat. 2010;119(2):347–356. [PubMed]
5. Crown J, Coate L, Keane M., et al. Randomized phase II study of pre-operative docetaxel, carboplatin with trastuzumab (TCH) and/or/lapatinib (L) in HER-2 positive (H+) breast cancer patients (BC pts). ICORG 10-05. Cancer Res. 2013;73(24 Suppl): abstract nr P4-12-25.

Articles from JNCI Journal of the National Cancer Institute are provided here courtesy of Oxford University Press